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A high-gradient magnetic separator that utilizes two kinds of plate including flat and lattice plates to provide a high-gradient magnetic field and separate magnetic particles from suspension was investigated in this paper. The separation of magnetic particles by in-situ observation has been carried out, and the results indicated that the magnetic particles formed shapes like a spike at the entrance of the separation system where magnetic particles were influenced by the magnetic field. Although long spike shapes were also observed at lower flow rates and lower magnetic field, the spikes appeared to be denser under the conditions of high magnetic field and high flow rates. The results achieved from in-situ observations can provide valuable information for the simulation of such a system. The two most important experimental variables, flow rate and distance between magnets, have been optimized for the separation of magnetic particles from suspension. The most efficient backwash system was also offered to reuse magnetic particles. The suggested system can be used to separate magnetic particles larger than 50 μm where using filter matrix is limited due to fast clogging. [ABSTRACT FROM AUTHOR]