1. Determination and Validation of a Single Laboratory Method for Coenzyme Q10 (Ubiquinone) by UHPLC.
- Author
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Manasyan, Louisa, Fang Xia, Koroma, Mohamed, Hines, Thomas, and Dennison, Martin
- Subjects
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COMPLEX matrices , *UBIQUINONES , *LIQUID chromatography-mass spectrometry , *SUSTAINABLE chemistry , *LIQUID chromatography , *RAW materials , *DETECTION limit - Abstract
A single laboratory validation study (SLV) was performed using an ultrahigh-pressure liquid chromatography (UHPLC) for the determination of coenzyme Q10 in a variety of formulations (raw materials, soft gel capsules, and in-process blend). The authors first developed an efficient extraction procedure to obtain coenzyme Q10 from finished products, and then used UHPLC to analyze the ingredient. Compared to the traditional highperformance liquid chromatography (HPLC) method, the UHPLC method reduced the total run time from 15 min to less than 2 min. The total amount of solvent usage was also reduced by 90%, which is a big contribution to green chemistry. The established UHPLC method exhibits good linearity, reproducibility, accuracy, recovery, and specificity. The limit of detection (LOD) and limit of quantification (LOQ) were also captured in the study, and have been proven suitable for monitoring the coenzyme Q10 in complex matrices. [ABSTRACT FROM AUTHOR]
- Published
- 2020