1. Evidence that a polyhexameric genome length is preferred, but not strictly required, for efficient mumps virus replication.
- Author
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Sauder, Christian J., Simonyan, Vahan, Ngo, Laurie, Karagiannis, Konstantinos, Cong, Yu, Zhang, Cheryl, Wang, Rong, Wu, Wells W., Malik, Tahir, and Rubin, Steven A.
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VIRAL replication , *MUMPS , *REPORTER genes , *GENE expression , *VIRAL genomes , *RNA polymerases - Abstract
Mumps virus (MuV) is postulated to adhere to the “rule of six” for efficient replication. To examine the requirement for MuV, minigenomes of nonpolyhexameric length (6 n −1 and 6 n +1) were analyzed. Expression of the reporter gene CAT was significantly reduced with minigenomes of nonpolyhexameric length compared to the wild type 6 n genome, and reduction was more pronounced for the 6 n −1 than for the 6 n +1 minigenome. That 6 n −1 genomes are impacted by nonconformance with the rule of six to a greater degree as compared to 6 n +1 genomes was also suggested with MuV derived from cDNA coding for 6 n +1 or 6 n −1 genomes. While viruses recovered from 6 n +1 cDNAs maintained a nonpolyhexameric genome length over multiple replication cycles, viruses rescued from the 6 n −1 cDNAs acquired length correcting mutations rapidly following rescue. Our data indicate that polyhexameric genomes are the preferred template for the MuV RNA polymerase, but that this requirement is not absolute. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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