40 results on '"Fang Xu"'
Search Results
2. Activation of autophagy inhibits the activation of NLRP3 inflammasome and alleviates sevoflurane-induced cognitive dysfunction in elderly rats
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Zhou, Junjie, Zhang, Chao, Fang, Xu, Zhang, Naixin, Zhang, Xiaoxi, and Zhu, Zhaoqiong
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- 2023
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3. A novel gene, TARDBP, and the protein it encodes can predict glioma patient prognosis and establish a prediction model
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Fang, Xu, Wu, Fan, and Jiang, Chen
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- 2023
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4. Genome-wide TCP transcription factors analysis provides insight into their new functions in seasonal and diurnal growth rhythm in Pinus tabuliformis
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Nie, Yu-meng, Han, Fang-xu, Ma, Jing-jing, Chen, Xi, Song, Yi-tong, Niu, Shi-Hui, and Wu, Harry X.
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- 2022
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5. A three-gene cluster in Trichoderma reesei reveals a potential role of dmm2 in DNA repair and cellulase production
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Cai, Wanchuan, Chen, Yumeng, Zhang, Lei, Fang, Xu, and Wang, Wei
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- 2022
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6. Gastrodin improves neuroinflammation-induced cognitive dysfunction in rats by regulating NLRP3 inflammasome
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Zheng, Xue, Gong, Taowu, Tang, Chunchun, Zhong, Yuanping, Shi, Lu, Fang, Xu, Chen, Dongqin, and Zhu, Zhaoqiong
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- 2022
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7. The difference of lipid profiles between psoriasis with arthritis and psoriasis without arthritis and sex-specific downregulation of methotrexate on the apolipoprotein B/apolipoprotein A-1 ratio
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Wang, Bing, Deng, Hui, Hu, Yao, Han, Ling, Huang, Qiong, Fang, Xu, Yang, Ke, Wu, Siyuan, Zheng, Zhizhong, Yawalkar, Nikhil, Zhang, Zhenghua, and Yan, Kexiang
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- 2022
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8. Radiomics nomogram for the preoperative prediction of lymph node metastasis in pancreatic ductal adenocarcinoma
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Bian, Yun, Guo, Shiwei, Jiang, Hui, Gao, Suizhi, Shao, Chengwei, Cao, Kai, Fang, Xu, Li, Jing, Wang, Li, Ma, Chao, Zheng, Jianming, Jin, Gang, and Lu, Jianping
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- 2022
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9. Umbilical cord blood cell characteristics in very preterm neonates for autologous cell therapy of preterm-associated complications.
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Zhuxiao, Ren, Jiangxue, Han, Yongsheng, Li, Jingjun, Pei, Shuo, Yang, Fang, Xu, Qi, Zhang, Shandan, Zhang, Chuan, Nie, and Jie, Yang
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CORD blood ,BLOOD cells ,CELLULAR therapy ,UMBILICAL cord clamping ,LEUCOCYTES ,NEWBORN infants ,LYMPHOCYTE count - Abstract
Background: There are emerging clinical evidence for umbilical cord blood mononuclear cells (UCBMNCs) intervention to improve preterm complications. The first critical step in cell therapy is to obtain high-quality cells. This retrospective study aimed to investigate the quantity and quality of UCBMNCs from very preterm infants (VPIs) for the purpose of autologous cell therapy in prevention and treatment of preterm complications. Methods: Very preterm infants (VPIs) born in Guangdong Women and Children Hospital from January 1, 2017, to December 8, 2022, from whom cord blood was successfully collected and separated for public or private banking, were enrolled. The UCBMNCs characters from route cord blood tests performed in cord blood bank, impact of perinatal factors on UCBMNCs, the relationship between UCBMNCs characteristics and preterm outcomes, and the correlation of UCBMNCs characteristics and peripheral blood cells in VPIs were analyzed. Results: Totally, 89 VPIs underwent UCB collection and processing successfully. The median cell number post processing was 2.6 × 10
8 . To infuse a dose of 5 × 107 cells/kg, only 3.4% of infants required a volume of more than 20 mL/kg, which exceeded the maximum safe volume limit for VPIs. However, when infusing 10 × 107 cells/kg, 25.8% of infants required a volume of more than 20 ml/kg volume. Antenatal glucocorticoids use and preeclampsia was associated with lower original UCBMNCs concentration. Both CD34+ hematopoietic stem cells (HSC) frequency and colony forming unit - granulocyte and macrophage (CFU-GM) number correlated negatively with gestational age (GA). UCBMNCs characters had no significant effect on preterm outcomes, whereas a significant positive correlation was observed between UCBMNCs concentration and total white blood cell, neutrophil, lymphocyte and PLT counts in peripheral blood. Conclusion: UCBMNCs collected from VPIs was feasible for autologous cell therapy in improving preterm complications. Setting the infusion dose of 5 × 107 cells/kg guaranteed a safe infusion volume in more than 95% of the targeted infants. UCBMNCs characters did not affect preterm complications; however, the effect of UCBMNCs concentration on peripheral blood classification count should be considered when evaluating the immunomodulation of UCBMNCs transfusion. [ABSTRACT FROM AUTHOR]- Published
- 2024
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10. AAA237, an SKP2 inhibitor, suppresses glioblastoma by inducing BNIP3-dependent autophagy through the mTOR pathway.
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Zhang, Yizhi, Li, Wan, Yang, Yihui, Zhang, Sen, Yang, Hong, Hao, Yue, Fang, Xu, Du, Guanhua, Shi, Jianyou, Wu, Lianqiu, and Wang, Jinhua
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GLIOBLASTOMA multiforme ,BRAIN tumors ,TUMOR growth ,AUTOPHAGY ,ANTINEOPLASTIC agents ,VASCULOGENIC mimicry ,GLIOMAS ,METHYLGUANINE - Abstract
Background: Glioblastoma (GBM) is the most common brain tumor with the worst prognosis. Temozolomide is the only first-line drug for GBM. Unfortunately, the resistance issue is a classic problem. Therefore, it is essential to develop new drugs to treat GBM. As an oncogene, Skp2 is involved in the pathogenesis of various cancers including GBM. In this study, we investigated the anticancer effect of AAA237 on human glioblastoma cells and its underlying mechanism. Methods: CCK-8 assay was conducted to evaluate IC
50 values of AAA237 at 48, and 72 h, respectively. The Cellular Thermal Shift Assay (CETSA) was employed to ascertain the status of Skp2 as an intrinsic target of AAA237 inside the cellular milieu. The EdU-DNA synthesis test, Soft-Agar assay and Matrigel assay were performed to check the suppressive effects of AAA237 on cell growth. To identify the migration and invasion ability of GBM cells, transwell assay was conducted. RT-qPCR and Western Blot were employed to verify the level of BNIP3. The mRFP-GFP-LC3 indicator system was utilized to assess alterations in autophagy flux and investigate the impact of AAA237 on the dynamic fusion process between autophagosomes and lysosomes. To investigate the effect of compound AAA237 on tumor growth in vivo, LN229 cells were injected into the brains of mice in an orthotopic model. Results: AAA237 could inhibit the growth of GBM cells in vitro. AAA237 could bind to Skp2 and inhibit Skp2 expression and the degradation of p21 and p27. In a dose-dependent manner, AAA237 demonstrated the ability to inhibit colony formation, migration, and invasion of GBM cells. AAA237 treatment could upregulate BNIP3 as the hub gene and therefore induce BNIP3-dependent autophagy through the mTOR pathway whereas 3-MA can somewhat reverse this process. In vivo, the administration of AAA237 effectively suppressed the development of glioma tumors with no side effects. Conclusion: Compound AAA237, a novel Skp2 inhibitor, inhibited colony formation, migration and invasion of GBM cells in a dose-dependent manner and time-dependent manner through upregulating BNIP3 as the hub gene and induced BNIP3-dependent autophagy through the mTOR pathway therefore it might be a viable therapeutic drug for the management of GBM. [ABSTRACT FROM AUTHOR]- Published
- 2024
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11. Spectrum of Germline RET variants identified by targeted sequencing and associated Multiple Endocrine Neoplasia type 2 susceptibility in China
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Qi, Xiao-Ping, Zhao, Jian-Qiang, Fang, Xu-Dong, Lian, Bi-Jun, Li, Feng, Wang, Hui-Hong, Cao, Zhi-Lie, Zheng, Wei-Hui, Cao, Juan, and Chen, Yu
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- 2021
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12. Recipient C7 rs9292795 genotype and the risk of hepatocellular carcinoma recurrence after orthotopic liver transplantation in a Han Chinese population
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Jiang, Zhongyi, Jiang, Qianwei, Fang, Xu, Wang, Pusen, Que, Weitao, Li, Hao, Yu, Yang, Liu, Xueni, Wang, Chunguang, and Zhong, Lin
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- 2021
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13. Identification of proteins associated with development of psoriatic arthritis in peripheral blood mononuclear cells: a quantitative iTRAQ-based proteomics study
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Zhu, Jie, Han, Ling, Liu, Ruilai, Zhang, Zhenghua, Huang, Qiong, Fang, Xu, Yang, Ke, Huang, Guiqin, Zheng, Zhizhong, Yawalkar, Nikhil, Deng, Hui, and Yan, Kexiang
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- 2021
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14. Comparative transcriptome analyses reveal two distinct transcriptional modules associated with pollen shedding time in pine
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Ma, Jing-Jing, Liu, Shuang-Wei, Han, Fang-Xu, Li, Wei, Li, Yue, and Niu, Shi-Hui
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- 2020
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15. miR-874 inhibits gastric cancer cell proliferation by targeting SPAG9
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Sun, Qin Hui, Yin, Zong Xiu, Li, Zhi, Tian, Shu Bo, Wang, Hong Chang, Zhang, Fang Xu, Li, Le Ping, Zheng, Chun Ning, and Kong, Shuai
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- 2020
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16. Diagnostic performance in T staging for patients with esophagogastric junction cancer using high-resolution MRI: a comparison with conventional MRI at 3 tesla
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Yuan, Yuan, Chen, Luguang, Ren, Shengnan, Wang, Zhen, Chen, Yukun, Jin, Aiguo, Li, Shuai, Fang, Xu, Wang, Tiegong, Bian, Yun, Yang, Qingsong, Bai, Chenguang, Hao, Qiang, and Lu, Jianping
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- 2019
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17. MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment
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Fang, Douglas D., Tang, Qiuqiong, Kong, Yanhui, Wang, Qixin, Gu, Jiaxing, Fang, Xu, Zou, Peng, Rong, Tao, Wang, Jingwen, Yang, Dajun, and Zhai, Yifan
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- 2019
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18. Use of fusion transcription factors to reprogram cellulase transcription and enable efficient cellulase production in Trichoderma reesei
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Wang, Fangzhong, Zhang, Ruiqin, Han, Lijuan, Guo, Wei, Du, Zhiqiang, Niu, Kangle, Liu, Yucui, Jia, Chunjiang, and Fang, Xu
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- 2019
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19. Mechanical ventilation-associated pneumonia caused by Acinetobacter baumannii in Northeast China region: analysis of genotype and drug resistance of bacteria and patients’ clinical features over 7 years
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Wei Xu, Xiao Xu, Tao Zhang, Salisu Rabiu Bilya, and Cai-Fang Xu
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Microbiology (medical) ,Acinetobacter baumannii ,Male ,Carbapenem ,medicine.medical_specialty ,China ,Genotype ,medicine.drug_class ,Antibiotics ,Tigecycline ,Drug resistance ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,Medical microbiology ,Drug Resistance, Bacterial ,medicine ,polycyclic compounds ,Humans ,Pharmacology (medical) ,Children ,biology ,business.industry ,Sulfamethoxazole ,Research ,Public Health, Environmental and Occupational Health ,Pneumonia, Ventilator-Associated ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,bacterial infections and mycoses ,Respiration, Artificial ,Infectious Diseases ,Amikacin ,Drug resistance gene ,Child, Preschool ,bacteria ,Female ,business ,medicine.drug ,Acinetobacter Infections - Abstract
Objective To investigate the clinical features and outcomes of patients with mechanical ventilation-associated pneumonia (VAP) caused by Acinetobacter baumannii (Ab), and to characterize the drug resistance of pathogenic strains and carbapenem resistance-associated genes. Methods Clinical data were collected from the PICU of Shengjing Hospital. Patients who met the diagnostic criteria of VAP and for whom Ab was a pathogen were selected as study participants. The patients were divided into carbapenem-resistant A. baumannii (CRAB) and carbapenem-sensitive A. baumannii (CSAB) groups. The genes closely associated with Ab resistance to carbapenems and the efflux pump-related genes were detected by real-time polymerase chain reaction, and results compared between the two groups. Results The total mechanical ventilation time and the administration time of antibiotics after a diagnosis of Ab infection were significantly higher in the CRAB group. And the CRAB group strains were only sensitive to amikacin, cephazolin, compound sulfamethoxazole, and tigecycline. Genetic test results indicated that IPM expression was not significantly different between two groups. The OXA-51 and OXA-23 in the CRAB group was markedly higher than that in the CSAB group, while OXA-24 expression was markedly lower. The expression of AdeABC and AdeFGH was significantly greater in the CRAB compared to CSAB group. Conclusion In pediatric patients with VAP caused by Ab infection, the detection rate of CRAB strains is far higher than that of CSAB strains; The abnormal expression of β-lactamase-producing genes (OXA-23, OXA-24, and OXA-51) and efflux pump-related genes (AdeABC and AdeFGH) is closely related to the production of CRAB.
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- 2021
20. The potential role of chemotaxis and the complement system in the formation and progression of thoracic aortic aneurysms inferred from the weighted gene coexpression network analysis
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Chuxiang Lei, Wei Wang, Dan Yang, Hui Zhang, Wenlin Chen, Lei Ji, Haoxuan Kan, Fang Xu, and Yuehong Zheng
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0301 basic medicine ,Mononuclear cell proliferation ,T cell ,lcsh:Medicine ,030204 cardiovascular system & hematology ,Biology ,Thoracic aortic aneurysm ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,CX3CR1 ,medicine ,Humans ,Gene Regulatory Networks ,Gene ,Weighted gene coexpression network analysis ,Aortic Aneurysm, Thoracic ,Research ,Chemotaxis ,Gene Expression Profiling ,lcsh:R ,General Medicine ,medicine.disease ,Complement system ,Immune infiltration ,030104 developmental biology ,medicine.anatomical_structure ,Gene Ontology ,Cancer research - Abstract
Background Thoracic aortic aneurysm (TAA) can be life-threatening due to the progressive weakening and dilatation of the aortic wall. Once the aortic wall has ruptured, no effective pharmaceutical therapies are available. However, studies on TAA at the gene expression level are limited. Our study aimed to identify the driver genes and critical pathways of TAA through gene coexpression networks. Methods We analyzed the genetic data of TAA patients from a public database by weighted gene coexpression network analysis (WGCNA). Modules with clinical significance were identified, and the differentially expressed genes (DEGs) were intersected with the genes in these modules. Gene Ontology and pathway enrichment analyses were performed. Finally, hub genes that might be driving factors of TAA were identified. Furthermore, we evaluated the diagnostic accuracy of these genes and analyzed the composition of immune cells using the CIBERSORT algorithm. Results We identified 256 DEGs and two modules with clinical significance. The immune response, including leukocyte adhesion, mononuclear cell proliferation and T cell activation, was identified by functional enrichment analysis. CX3CR1, C3, and C3AR1 were the top 3 hub genes in the module correlated with TAA, and the areas under the curve (AUCs) by receiver operating characteristic (ROC) analysis of all the hub genes exceeded 0.7. Finally, we found that the proportions of infiltrating immune cells in TAA and normal tissues were different, especially in terms of macrophages and natural killer (NK) cells. Conclusion Chemotaxis and the complement system were identified as crucial pathways in TAA, and macrophages with interactive immune cells may regulate this pathological process.
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- 2021
21. LncRNA SNHG14 regulates the DDP-resistance of non-small cell lung cancer cell through miR-133a/HOXB13 pathway
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Yan Xu, Li Xu, Min Yang, Bolin Chen, Jia Li, and Fang Xu
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Pulmonary and Respiratory Medicine ,Lung Neoplasms ,endocrine system diseases ,Cell ,MiR-133a ,Apoptosis ,NSCLC ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,Medicine ,Humans ,LncRNA SNHG14 ,030304 developmental biology ,Cell Proliferation ,lcsh:RC705-779 ,A549 cell ,Homeodomain Proteins ,0303 health sciences ,Gene knockdown ,medicine.diagnostic_test ,business.industry ,Cell growth ,lcsh:Diseases of the respiratory system ,Cell cycle ,respiratory system ,respiratory tract diseases ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,medicine.anatomical_structure ,Cell culture ,A549 Cells ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer research ,RNA, Long Noncoding ,Cisplatin ,business ,HOXB13 ,DDP-resistance ,Research Article ,Signal Transduction - Abstract
Background Recently, long non-coding RNAs (lncRNAs) have been reported to be involved in regulating chemo-resistance of NSCLC, however, the role of lncRNA SNHG14 in the DDP-resistance of NSCLC remains unexplored. Methods Relative expression of SNHG14, HOXB13 and miR-133a in DDP-resistant A549 (A549/DDP) cell and its parental cell A549 were measured using qRT-PCR. Cell proliferation viability of indicated A549/DDP cell was estimated via CCK-8 and colony formation experiments. Cell cycle and apoptosis were analyzed through flow cytometry. Expression of apoptosis-related protein and HOXB13 were detected via western blot. The interaction among SNHG14, HOXB13 and miR-133a was predicted by bioinformatics and validated by dual-luciferase reporter assay. Results LncRNA SNHG14 and HOXB13 were upregulated while miR-133a was downregulated in A549/DDP cell line compared to A549 cell line. SNHG14 knockdown or miR-133a overexpression was demonstrated to increase the DDP-sensitivity of A549/DDP cells. SNHG14 was revealed to compete with HOXB13 for miR-133a binding in A549/DDP cells. Inhibition of miR-133a in A549 cells could reverse the promotive effects of SNHG14 knockdown on DDP-sensitivity, as well as the inhibitory effects on HOXB13 expression. HOXB13 overexpression was revealed to abolish the enhanced effects of miR-133a on the sensitivity of A549/DDP cell to DDP. Conclusion Our findings demonstrated that SNHG14 was involved in the development of DDP-resistance of A549/DDP cells through miR-133a/HOXB13 axis, which may present a path to novel therapeutic stratagems for DDP resistance of NSCLC.
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- 2020
22. Mdivi-1, a mitochondrial fission inhibitor, modulates T helper cells and suppresses the development of experimental autoimmune encephalomyelitis
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Yan-Hua Li, Bogoljub Ciric, Abdolmohamad Rostami, Zhi Chai, Guo-Bin Song, Rui-Lan Li, Man-Luan Sun, Mark T. Curtis, Fang Xu, Cun-Gen Ma, Guang-Xian Zhang, Min-Fang Guo, and Rodolfo Thomé
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0301 basic medicine ,Encephalomyelitis, Autoimmune, Experimental ,T cell ,Immunology ,T cells ,Inflammation ,Lymphocyte Activation ,Mitochondrial Dynamics ,lcsh:RC346-429 ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,DNM1L ,Myelin ,Mice ,0302 clinical medicine ,Immune system ,medicine ,Animals ,Enzyme Inhibitors ,lcsh:Neurology. Diseases of the nervous system ,Quinazolinones ,Experimental autoimmune encephalomyelitis ,Chemistry ,General Neuroscience ,Research ,FOXP3 ,RNA-Binding Proteins ,T-Lymphocytes, Helper-Inducer ,medicine.disease ,3. Good health ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Neurology ,Mdivi-1 ,Mitochondrial fission ,Female ,medicine.symptom ,030217 neurology & neurosurgery ,Dynamin-related protein 1 - Abstract
Background Unrestrained activation of Th1 and Th17 cells is associated with the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). While inactivation of dynamin-related protein 1 (Drp1), a GTPase that regulates mitochondrial fission, can reduce EAE severity by protecting myelin from demyelination, its effect on immune responses in EAE has not yet been studied. Methods We investigated the effect of Mdivi-1, a small molecule inhibitor of Drp1, on EAE. Clinical scores, inflammation, demyelination and Drp1 activation in the central nervous system (CNS), and T cell responses in both CNS and periphery were determined. Results Mdivi-1 effectively suppressed EAE severity by reducing demyelination and cellular infiltration in the CNS. Mdivi-1 treatment decreased the phosphorylation of Drp1 (ser616) on CD4+ T cells, reduced the numbers of Th1 and Th17 cells, and increased Foxp3+ regulatory T cells in the CNS. Moreover, Mdivi-1 treatment effectively inhibited IFN-γ+, IL-17+, and GM-CSF+ CD4+ T cells, while it induced CD4+ Foxp3+ regulatory T cells in splenocytes by flow cytometry. Conclusions Together, our results demonstrate that Mdivi-1 has therapeutic potential in EAE by modulating the balance between Th1/Th17 and regulatory T cells.
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- 2019
23. Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
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Longlong Fan, Weiwei Hu, Jun Fang, Hong Shen, Feng Zhou, Jin-fang Xu, and Zhonghao Cheng
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0301 basic medicine ,Male ,Cancer Research ,KNG1 ,Angiogenesis ,Mice, Nude ,Apoptosis ,Transfection ,lcsh:RC254-282 ,03 medical and health sciences ,Mice ,Glioma ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Viability assay ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,TUNEL assay ,Chemistry ,Brain Neoplasms ,Kininogens ,Research ,Cell cycle ,Middle Aged ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,XIAP ,030104 developmental biology ,Oncology ,Cancer research ,Heterografts ,Female - Abstract
Background Glioma is the most common primary central nervous system tumor derived from glial cells. Kininogen-1 (KNG1) can exert antiangiogenic properties and inhibit proliferation of endothelial cells. The effect of KNG1 on the glioma is rarely reported, so our purpose in to explore its mechanism in glioma cells. Methods The differentially expressed genes (DEGs) were identified based on The Cancer Genome Atlas (TCGA) database. The KNG1-vector was transfected into the two glioma cells. The viability, apoptosis and cell cycle of glioma cells and microvessel density (MVD) were detected by cell counting kit-8 assay, flow cytometry and immunohistochemistry, respectively. The expression were measured by quantitative real-time PCR and Western blot, respectively. A tumor mouse model was established to determine apoptosis rate of brain tissue by terminal deoxynucleotidyl transfer-mediated dUTP nick end labeling (TUNEL) analysis. Results KNG1 was identified as the core gene and lowly expressed in the glioma cells. Overexpression of KNG1 inhibited cell viability and angiogenesis of glioma cells. Overexpression of KNG1 promoted the apoptosis and G1 phase cell cycle arrest of glioma cells. Moreover, the expressions of VEGF, cyclinD1, ki67, caspase-3/9 and XIAP were regulated by overexpression of KNG1. In addition, overexpression of KNG1 inhibited the activity of PI3K/Akt. Furthermore, overexpression of KNG1 decreased the tumor growth and promoted the apoptosis of decreased by overexpression of KNG1 in vivo. . Conclusions Overexpression of KNG1 suppresses glioma progression by inhibiting the proliferation and promoting apoptosis of glioma cells, providing a therapeutic strategy for the malignant glioma.
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- 2018
24. Recipient C7 rs9292795 genotype and the risk of hepatocellular carcinoma recurrence after orthotopic liver transplantation in a Han Chinese population.
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Zhongyi Jiang, Qianwei Jiang, Xu Fang, Pusen Wang, Weitao Que, Hao Li, Yang Yu, Xueni Liu, Chunguang Wang, Lin Zhong, Jiang, Zhongyi, Jiang, Qianwei, Fang, Xu, Wang, Pusen, Que, Weitao, Li, Hao, Yu, Yang, Liu, Xueni, Wang, Chunguang, and Zhong, Lin
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HEPATOCELLULAR carcinoma ,LIVER transplantation ,OVERALL survival ,SINGLE nucleotide polymorphisms ,PROPORTIONAL hazards models ,GENOTYPES ,COMPLEMENT (Immunology) ,LIVER tumors ,GENETIC polymorphisms ,CANCER relapse ,LOGISTIC regression analysis - Abstract
Background: Complement component(C7) gene has been shown to influence the prognosis in Hepatocellular carcinoma (HCC) patients. The association between C7 and HCC recurrence after orthotopic liver transplantation (OLT), however, is still unknown. The purpose of this study was to evaluate whether the donor and recipient C7 gene polymorphisms are related to HCC recurrence after OLT in the Han Chinese population.Methods: A total of 73 consecutive patients with HCC who had undergone OLT, both donors and recipients, were involved in this research. A single nucleotide polymorphism of C7, rs9292795, was genotyped using Sequenom MassARRAY in the cohort. The expression of C7 and the association between C7 gene polymorphisms and HCC recurrence following OLT were analyzed by bioinformatics and statistical analysis, respectively.Results: As shown in database, the expression of C7 was higher in HCC tissues than that in normal tissues, and represented a worse prognosis. We also found that recipient C7 rs9292795 polymorphism, rather than the donor, was significantly associated with HCC recurrence after OLT. Multivariate logistic regression analysis confirmed that TNM stage (P = 0.001), Milan criteria (P = 0.000) and recipient rs9292795 genotype (TT vs AA/AT, P = 0.008) were independent risk factors for HCC recurrence. Furthermore, the recipient carrying AA/AT showed higher recurrence-free survival (RFS) and overall survival (OS) than that carrying TT (P < 0.05). In Cox proportional hazards model, TNM stage, recipient rs9292795 genotype, and Milan criteria were identified as independent factors for RFS and OS (P < 0.05) as well as pre-OLT serum alpha fetoprotein (AFP) level was associated with OS (P < 0.05).Conclusions: Recipient C7 rs9292795 gene polymorphism is related to the recurrence of HCC after OLT, which may be a helpful prognostic marker for HCC patients who receive OLT. [ABSTRACT FROM AUTHOR]- Published
- 2021
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25. Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Beijing, China: 2006 versus 2012
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Ying-jia Li, Qing-qin Yin, Qinjing Li, Hairong Huang, Adong Shen, Weiwei Jiao, Lin Sun, Jieqiong Li, and Fang Xu
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0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,China ,Tuberculosis ,Extensively Drug-Resistant Tuberculosis ,030106 microbiology ,Antitubercular Agents ,Drug resistance ,Microbial Sensitivity Tests ,Microbiology ,Mycobacterium tuberculosis ,03 medical and health sciences ,0302 clinical medicine ,Bacterial Proteins ,Mutation Rate ,Internal medicine ,Drug Resistance, Multiple, Bacterial ,Tuberculosis, Multidrug-Resistant ,medicine ,Prevalence ,Humans ,Molecular characteristics ,030212 general & internal medicine ,Ethambutol ,Drug-resistant ,biology ,Molecular epidemiology ,Isoniazid ,Extensively drug-resistant tuberculosis ,biology.organism_classification ,medicine.disease ,Immunology ,Rifampicin ,medicine.drug ,Research Article - Abstract
Background As the epidemic of MDR-TB and XDR-TB becomes increasingly severe, it is important to determine the clinical characteristics and molecular epidemiology of MDR-TB and XDR-TB. Recently, many studies have shown that clinical features and molecular characteristics of drug-resistant strains vary in different geographical areas, however, further information is needed to assess the dynamic evolution of drug-resistant TB. Comparative studies between different time periods are necessary to elucidate the development of drug-resistant TB. Results A total of 255 and 537 strains were collected from Beijing Chest Hospital in 2006 and in 2012, respectively. Drug-resistance rates and mutations associated with resistance to first-line anti-tuberculosis (TB) drugs were compared. The overall rate of drug resistance among strains of TB in 2012 was 54.4 %, significantly higher than that in 2006 (34.9 %, P
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- 2016
26. NADPH oxidase p47phox siRNA attenuates adventitial fibroblasts proliferation and migration in apoE(-/-) mouse
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Yong Cui, Li Zhang, Yejia Hu, Jie Qi, Lei Shi, Wei Liu, Hongjing Cai, Weichen Wang, Ying Liu, and Fang Xu
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Apolipoprotein E ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,Mice ,Apolipoproteins E ,Cell Movement ,Adventitia ,medicine ,Animals ,RNA, Small Interfering ,Cell Proliferation ,Medicine(all) ,Neointimal hyperplasia ,Mice, Knockout ,Messenger RNA ,Gene knockdown ,Adventitia fibroblasts ,NADPH oxidase ,biology ,Base Sequence ,Biochemistry, Genetics and Molecular Biology(all) ,Superoxide ,Cell growth ,Reverse Transcriptase Polymerase Chain Reaction ,Research ,p47phox ,NADPH Oxidases ,General Medicine ,ApoE(-/-) ,Fibroblasts ,medicine.disease ,Atherosclerosis ,Molecular biology ,Mice, Inbred C57BL ,medicine.anatomical_structure ,chemistry ,biology.protein - Abstract
Reactive oxide species (ROS) derived from NADPH oxidases is involved in atherosclerosis. However, as a key component of NADPH oxidase, how p47phox regulates NADPH oxidases activity, ROS production and adventitial fibroblasts (AFs) function remains unclear. p47phox in aortic arteries of apoE(-/-) mice fed with hyperlipid diet was detected by immunohistochemistry. NADPH oxidase activity, superoxide anion (O2 −) generation and p47phox expression were analyzed in primary AFs treated by diphenyleneiodonium (DPI). The proliferation and migration of AFs were also analyzed. p47phox expression was low in the aortic adventitia but high in the site of intimal injury with continuous hyperlipidic diet. Compared to AFs from wild-type mice, AFs derived from apoE(-/-) mice exhibited elevated NADPH oxidase activity, O2 − production and higher mRNA and protein levels of p47phox, correlated with increased capability of proliferation and migration. DPI inhibited NADPH oxidase activity and AFs proliferation and migration in a dose-dependent manner. In addition, siRNA mediated knockdown of p47phox attenuated the proliferation and migration of AFs derived from apoE(-/-) mice. p47phox plays a critical role in the regulation of adventitial fibroblast proliferation and migration and may be a new therapeutic target for neointimal hyperplasia.
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- 2015
27. Family support, discrimination, and quality of life among ART-treated HIV-infected patients: a two-year study in China.
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Jun-Fang Xu, Zhong-Qiang Ming, Yu-Qian Zhang, Pei-Cheng Wang, Jun Jing, and Feng Cheng
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HIGHLY active antiretroviral therapy , *QUALITY of life , *HIV - Abstract
Background: By September 2016, approximately 653,865 people in China were living with HIV/AIDS (PLWHA) and 492,725 people were receiving antiretroviral therapy (ART). PLWHA frequently experience discrimination in all domains of their personal and social lives. The World Health Organization includes discrimination in its list of social determinants of health factors that have been linked to poor physical and psychological health. This paper identifies the family support enjoyed and discrimination faced by people infected with HIV and examines the effect they have on patients' quality of life (QOL) as they undergo ART in China. Methods: We conducted this observational cohort study of ART-treated patients with HIV in Guangxi Province using a questionnaire survey at baseline, 6, 12, and 24 months, starting in 2010. Descriptive analysis was used to describe the demographic characteristics (e.g., age, sex, educational level, marital status, and employment status) of participants. Generalized estimating equations (GEE) were employed to examine the relationships between family support, discrimination, and QOL. Results: In the study, 90.4% (n = 281) of patients received family support at baseline, here defined as the initiation of ART, 91.8% (n = 244) received family support 6 months into ART, 95.5% (n = 220) at 12 months, and 94.3% (n = 230) at 24 months. The proportion of patients who did not feel discriminated against by their families was 87.2% (n = 274) at baseline, 90.4% (n = 229) 6 months into ART, 90.0% (n = 210) at 12 months, and 94.5% (n = 219) at 24 months. Patients' overall QOL scores were positively associated with having received family support (OR = 2.74, P = 0.040, 95% CI: 1.68-4.47), not feeling discriminated against by their families (OR = 1.3, P = 0.041, 95% CI: 1.07-1.59) or discrimination from patients themselves, including never experiencing fear of abandonment by family (OR = 2.05, P = 0.025, 95% CI: 1.49-2.82). Conclusions: Family support along with no or minimal discrimination was found to contribute to QOL among people infected with HIV. Their overall QOL tended to improve significantly as ART continued. This suggests that strategies meant to improve and strengthen family support, care for PLWHA, and promote HIV screening among high-risk populations should be explored by both policy makers and researchers. [ABSTRACT FROM AUTHOR]
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- 2017
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28. The relationship between changes of cervical sagittal alignment after anterior cervical discectomy and fusion and spino-pelvic sagittal alignment under roussouly classification: a four-year follow-up study.
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Dong-Ning Huang, Miao Yu, Nan-Fang Xu, Mai Li, Shao-Bo Wang, Yu Sun, Liang Jiang, Feng Wei, Xiao-Guang Liu, Zhong-Jun Liu, Huang, Dong-Ning, Yu, Miao, Xu, Nan-Fang, Li, Mai, Wang, Shao-Bo, Sun, Yu, Jiang, Liang, Wei, Feng, Liu, Xiao-Guang, and Liu, Zhong-Jun
- Subjects
DISCECTOMY ,DEGENERATION (Pathology) ,SPONDYLOSIS ,LORDOSIS ,PATIENTS ,THERAPEUTICS ,CERVICAL vertebrae ,LONGITUDINAL method ,PELVIC bones ,SPINAL fusion ,TIME ,RETROSPECTIVE studies ,SURGERY - Abstract
Background: Anterior cervical discectomy and fusion (ACDF) is widely used in the treatment of cervical degenerative disease; however, the variation of cervical sagittal alignment changes after ACDF has been rarely explored. The purpose of this study is to determine the relationship between changes of cervical sagittal alignment after ACDF and spino-pelvic sagittal alignment under Roussouly classification.Methods: A cohort of 133 Chinese cervical spondylotic patients who received ACDF from 2011 to 2012 was recruited. All patients were categorized with Roussouly Classification. Lateral X-ray images of global spine were obtained, and preoperative and postoperative parameters were measured and analyzed, including C2-C7 angles (C2-C7), C0-C7 angles (C0-C7), external auditory meatus (EAM) tilt, sacral slope (SS), thoracic kyphosis (TK), lumbar lordosis (LL), spinal sacral angles (SSA), Superior adjacent inter-vertebral angle (SAIV), inferior adjacent inter-vertebral angle (IAIV) and et al. The Wilcoxon signed-rank test was used for intragroup comparisons preoperatively and at postoperative 48 months.Results: Among the parameters, C2-C7 and C0-C7 showed significant increase, while EAM TK, and IAIV decreased significantly. In type I, EAM and TK decreased significantly, however SS showed a significant increase; in type II, TK showed a significant decrease, but SSA showed a significant increase; in type III, a significant increase of C0-C7 was observed with a significant decrease in EAM, nevertheless, LL, SS and SSA showed significant decreases; and in type IV, C2-C7 showed a significant increase and EAM decreased significantly. The percentage of lordotic alignment in cervical spine increased, which was presenting in type I, III and IV. Nevertheless, the amount of patients with straight cervical alignment increased in type II.Conclusion: The backward movement of head occurs is the compensatory mechanism in cervical sagittal alignment modifications after ACDF. The compensatory alteration of spino-pelvic sagittal alignment varied in different Roussouly type. [ABSTRACT FROM AUTHOR]- Published
- 2017
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29. Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Beijing, China: 2006 versus 2012.
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Qing-Qin Yin, Wei-Wei Jiao, Qin-Jing Li, Fang Xu, Jie-Qiong Li, Lin Sun, Ying-Jia Li, Hai-Rong Huang, and A-Dong Shen
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MOLECULAR epidemiology ,DISEASE prevalence ,MYCOBACTERIUM tuberculosis ,DRUG resistance in bacteria ,GENETIC mutation - Abstract
Background: As the epidemic of MDR-TB and XDR-TB becomes increasingly severe, it is important to determine the clinical characteristics and molecular epidemiology of MDR-TB and XDR-TB. Recently, many studies have shown that clinical features and molecular characteristics of drug-resistant strains vary in different geographical areas, however, further information is needed to assess the dynamic evolution of drug-resistant TB. Comparative studies between different time periods are necessary to elucidate the development of drug-resistant TB. Results: A total of 255 and 537 strains were collected from Beijing Chest Hospital in 2006 and in 2012, respectively. Drug-resistance rates and mutations associated with resistance to first-line anti-tuberculosis (TB) drugs were compared. The overall rate of drug resistance among strains of TB in 2012 was 54.4 %, significantly higher than that in 2006 (34.9 %, P < 0.001). Rates of resistance to each first-line drug (isoniazid, rifampicin, streptomycin and ethambutol) and to second-line drug ofloxacin increased significantly from 2006 to 2012. The overall MDR rate also increased significantly from 2006 (14.9 %) to 2012 (27.0 %). The rate of MDR increased significantly between these two time periods in previously treated cases (P = 0.023) but not in new cases (P = 0.073), and the rate of XDR was similar in new cases at the two time periods, but was marginally higher in 2012 in previously treated cases (P = 0.056). Previous treatment was found to be a risk factor for drug-resistant TB, especially for MDR-TB. In addition, the proportion of drug resistant isolates in which katG, the mabA-inhA promoter, oxyR-ahpC intergenic region, rpoB, rpsL, and embB were mutated was similar in 2006 and 2012, however patterns of mutation in these loci were more diverse in 2012 compared to 2006. Conclusions: Our data suggests that the prevalence of drug resistant TB remains high in Beijing, China, and that increasing rates of resistance in M. tuberculosis to all anti-TB drugs should be considered when choosing an optimal anti-TB regimen. Moreover, acquired multi-drug resistance may play a primary role in the MDR-TB epidemic in Beijing, China. Consequently, this highlights the importance of an earlier start to effective and supervised treatment in order to reduce the burden of retreatment. [ABSTRACT FROM AUTHOR]
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- 2016
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30. Clinical value of endoluminal ultrasonography in the diagnosis of rectovaginal fistula.
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Hao-Qiang Yin, Chen Wang, Xin Peng, Fang Xu, Ya-Juan Ren, Yong-Qing Chao, Jin-Gen Lu, Song Wang, and Hu-Sheng Xiao
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RECTOVAGINAL fistula ,RECTAL diseases ,PUBLIC health ,DIAGNOSTIC ultrasonic imaging ,PREOPERATIVE care ,POSTOPERATIVE care ,DIAGNOSIS - Abstract
Background: Rectovaginal fistula (RVF) refers to a pathological passage between the rectum and vagina, which is a public health challenge. This study was aimed to explore the clinical value of endoluminal biplane ultrasonography in the diagnosis of rectovaginal fistula (RVF). Methods: Thirty inpatients and outpatients with suspected RVF from January 2006 to June 2013 were included in the study, among whom 28 underwent surgical repair. All 28 patients underwent preoperative endoluminal ultrasonography, and the obtained diagnostic results were compared with the corresponding surgical results. Results: All of the internal openings located at the anal canal and rectum of the 28 patients and confirmed during surgery were revealed by preoperative endosonography, which showed a positive predictive value of 100%. Regarding the 30 internal openings located in the vagina during surgery, the positive predictive value of preoperative endosonography was 93%. The six cases of simple fistulas confirmed during surgery were revealed by endosonography; for the 22 cases of complex fistula confirmed during surgery, the positive predictive value of endosonography was 90%. Surgery confirmed 14 cases of anal fistula and 14 cases of RVF, whereas preoperative endoluminal ultrasonography suggested 16 cases of anal fistula and 12 cases of RVF, resulting in positive predictive values of 92.3 and 93%, respectively. Conclusion: The use of endoluminal biplane ultrasonography in the diagnosis of RVF can accurately determine the internal openings in the rectum or vagina and can relatively accurately identify concomitant branches and abscesses located in the rectovaginal septum. Thus, it is a good imaging tool for examining internal and external anal sphincter injuries and provides useful information for preoperative preparation and postoperative evaluation. [ABSTRACT FROM AUTHOR]
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- 2016
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31. Comparison of first-line chemotherapy based on irinotecan or other drugs to treat non-small cell lung cancer in stage IIIB/IV: a systematic review and meta-analysis.
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Xue-Qin Yang, Chong-Yi Li, Ming-Fang Xu, Hong Zhao, Dong Wang, Yang, Xue-Qin, Li, Chong-Yi, Xu, Ming-Fang, Zhao, Hong, and Wang, Dong
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CANCER chemotherapy ,IRINOTECAN ,CANCER treatment ,NON-small-cell lung carcinoma ,SYSTEMATIC reviews ,DRUG efficacy ,DRUG side effects - Abstract
Background: To compare the efficacy and toxicity of irinotecan-based chemotherapy (IBC) and non-irinotecan-based chemotherapy (NIBC) as first-line treatment for stage IIIB/IV non-small cell lung cancer (NSCLC).Methods: PubMed, EMBASE, the Cochrane Central Register of Controlled Trials (CENTRAL), abstracts from the annual meetings of ASCO and the ESMO up to 2014 were searched for randomized controlled trials (RCTs) that compared IBC with NIBC. Data on overall survival (OS) and progression-free survival (PFS) were meta-analyzed to provide hazard ratios (HRs), while data on overall response rate (ORR) and frequencies of toxicity were meta-analyzed to provide relative risk ratios (RR).Results: Seven RCTs (6 RCTs from Asian population and 1 from non-Asian population) involving 1473 patients with previously untreated stage IIIB/IV NSCLC were included in the meta-analysis. IBC and NIBC were associated with similar ORR (RR: 1.08, 95%CI: 0.94 to 1.23, p=0.30), OS (HR: 0.97, 95%CI: 0.88 to 1.07, p=0.56), and PFS (HR: 1.02, 95%CI: 0.97 to 1.08, p=0.38). However, the subgroups between Asian and non-Asian patients differed significantly in OS (HR: 0.94 vs 1.87, p=0.007). There was no significant difference for hematological toxicity (RR: 0.79, 95%CI: 0.60 to 1.04, p=0.09) and significant worse for non-hematological toxicity (RR: 2.28, 95%CI: 1.60 to 3.24, p<0.001), when IBC compared to NIBC.Conclusions: As the available evidence suggests that IBC and NIBC are equivalent in terms of ORR, PFS, OS, at least in Asian patients, we recommend that IBC be considered as a first-line treatment in Asian patients with stage IIIB/IV NSCLC. However, the non-hematological toxicity of IBC must be considered. [ABSTRACT FROM AUTHOR]- Published
- 2015
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32. Treatment of gallbladder stone with common bile duct stones in the laparoscopic era.
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Wei-jie Zhang, Gui-fang Xu, Qin Huang, Kun-lun Luo, Zhi-tao Dong, Jie-ming Li, Guo-zhong Wu, and Wen-xian Guan
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GALLBLADDER ,BILIARY tract ,BILE ducts ,BODY fluids ,LAPAROSCOPY - Abstract
Background: Laparoscopic common bile duct exploration (LCBDE) for stone can be carried out by either laparoscopic transcystic stone extraction (LTSE) or laparoscopic choledochotomy (LC). It remains unknown as to which approach is optimal for management of gallbladder stone with common bile duct stones (CBDS) in Chinese patients. Methods: From May 2000 tofebruary 2009, we prospective treated 346 consecutive patients with gallbladder stones and CBDS with laparoscopic cholecystectomy and LCBDE. Intraoperative findings, postoperative complications, postoperative hospital stay and costs were analyzed. Results: Because of LCBDE failure,16 cases (4.6%) required open surgery. Of 330 successful LCBDE-treated patients, 237 underwent LTSE and 93 required LC. No mortality occurred in either group. The bile duct stone clearance rate was similar in both groups. Patients in the LTSE group were significantly younger and had fewer complications with smaller, fewer stones, shorter operative time and postoperative hospital stays, and lower costs, compared to those in the LC group. Compared with patients with T-tube insertion, patients in the LC group with primary closure had shorter operative time, shorter postoperative hospital stay, and lower costs. Conclusions: In cases requiring LCBDE, LTSE should be the first choice, whereas LC may be restricted to large, multiple stones. LC with primary closure without external drainage of the CBDS is as effective and safe as the T-tube insertion approach. [ABSTRACT FROM AUTHOR]
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- 2015
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33. NADPH oxidase p47phox siRNA attenuates adventitial fibroblasts proliferation and migration in apoE(-/-) mouse.
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Fang Xu, Ying Liu, Lei Shi, Wei Liu, Li Zhang, Hongjing Cai, Jie Qi, Yong Cui, Weichen Wang, and Yejia Hu
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NADPH oxidase , *SMALL interfering RNA , *FIBROBLASTS , *CELL proliferation , *ATHEROSCLEROSIS , *IMMUNOHISTOCHEMISTRY , *DIPHENYLENEIODONIUM - Abstract
Background: Reactive oxide species (ROS) derived from NADPH oxidases is involved in atherosclerosis. However, as a key component of NADPH oxidase, how p47phox regulates NADPH oxidases activity, ROS production and adventitial fibroblasts (AFs) function remains unclear. Methods: p47phox in aortic arteries of apoE(-/-) mice fed with hyperlipid diet was detected by immunohistochemistry. NADPH oxidase activity, superoxide anion (O2-) generation and p47phox expression were analyzed in primary AFs treated by diphenyleneiodonium (DPI). The proliferation and migration of AFs were also analyzed. Results: p47phox expression was low in the aortic adventitia but high in the site of intimal injury with continuous hyperlipidic diet. Compared to AFs from wild-type mice, AFs derived from apoE(-/-) mice exhibited elevated NADPH oxidase activity, O2- production and higher mRNA and protein levels of p47phox, correlated with increased capability of proliferation and migration. DPI inhibited NADPH oxidase activity and AFs proliferation and migration in a dose-dependent manner. In addition, siRNA mediated knockdown of p47phox attenuated the proliferation and migration of AFs derived from apoE(-/-) mice. Conclusion: p47phox plays a critical role in the regulation of adventitial fibroblast proliferation and migration and may be a new therapeutic target for neointimal hyperplasia. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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34. Increased of serum high-mobility group box chromosomal protein 1 correlated with intestinal mucosal barrier injury in patients with severe acute pancreatitis.
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Gui-fang Xu, Ming Guo, Zhi-qiang Tian, Guo-zhong Wu, Xiao-ping Zou, and Wei-jie Zhang
- Subjects
- *
PANCREATITIS diagnosis , *INTESTINAL mucosa injuries , *CHI-squared test , *STATISTICAL correlation , *FISHER exact test , *INFECTION , *OXIDOREDUCTASES , *PANCREATITIS , *PROTEINS , *STATISTICS , *T-test (Statistics) , *COMORBIDITY , *DATA analysis , *SEVERITY of illness index , *DATA analysis software , *DESCRIPTIVE statistics , *DISEASE complications - Abstract
Background Secondary infections are the leading cause of death in patients with severe acute pancreatitis (SAP). The gut represents the main source of pancreatic contamination and related septic complications. High-mobility group box chromosomal protein 1 (HMGB1) was recently identified to play an important role in the SAP intestinal mucosal barrier dysfunction. Objective To investigate the correlation of high-mobility group box 1 (HMGB1) with intestinal barrier injury and infections in patients with severe acute pancreatitis (SAP). Methods The serum levels of HMGB1, amylase, lipase, and biochemical indicators were measured in 80 patients with SAP at the time of admission. Furthermore, relationship between their serum HMGB1 levels and intestinal barrier injury, infection and other clinical factors were analyzed. Results The mean value of serum HMGB1 levels was significantly higher in patients with SAP (6.02 ± 2.42 ng/mL) than that in healthy volunteers (1.87 ± 0.63 ng/mL). Serum HMGB1 levels were significantly positively correlated with the Ranson score. The HMGB1 levels were higher in patients with infection during the clinical course, the HMGB1 levels in non-survivors were higher than those in survivors, and positively correlated with DAO activity, L/M ratio, the concentration of endotoxin (R = 0.484, P <0.01). Conclusions: HMGBl level of patients with severe acute pancreatitis was significantly increased, and can be used as an important indicator to determine the intestinal barrier dysfunction and infection. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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35. Biomechanical testing of a unique built-in expandable anterior spinal internal fixation system.
- Author
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Chu-Song Zhou, Yan-Fang Xu, Yu Zhang, Zhong Chen, and Hai Lv
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INTERNAL fixation in fractures , *SPINAL surgery , *BIOMECHANICS , *OSTEOTOMY , *BONE screws - Abstract
Background: Expandable screws have greater pullout strength than conventional screws. The purpose of this study was to compare the biomechanical stability provided by a new built-in expandable anterior spinal fixation system with that of 2 commonly used anterior fixation systems, the ZPlate and the Kaneda, in a porcine partial vertebral corpectomy model. Methods: Eighteen porcine thoracolumbar spine specimens were randomly divided into 3 groups of 6 each. A vertebral wedge osteotomy was performed by removing the anterior 2/3 of the L1 vertebral body and the T15/L1 disc. Vertebrae were fixed with the Z-Plate, Kaneda, or expandable fixation system. The 3-dimensional spinal range of motion (ROM) of specimens in the intact state (prior to osteotomy), injured state (after osteotomy), and after internal fixation were recorded. The pullout strength and maximum torque of common anterior screws, the expandable anterior fixation screw unexpanded, and the expandable anterior fixation screw expanded was tested. Results: After internal fixation, the expandable device and Z-plate system exhibited higher left bending motion than the Kaneda system (5.50° and 5.37° vs. 5.04, p = 0.001 and 0.008, respectively), and the Z-plate and Kaneda groups had significantly higher left axial and right axial rotation ROM as compared to the expandable device group (left axial rotation: 5.23° and 5.02° vs. 4.53°; right axial rotation: 5.23° and 5.08° vs. 4.49°). The maximum insertion torque of the expandable device was significantly greater than of a common screw (5.10 vs. 3.75 Ns). The maximum pullout force of the expandable device expanded was significantly higher than that of the common screw and the expandable device unexpanded (3,035.48 N vs. 1,827.38 N and 2,333.49 N). Conclusions: The built-in anterior fixation system provides better axial rotational stability as compared to the other 2 systems, and greater maximum torque and pullout strength than a common fixation screw. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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36. Multiplex ligation-dependent probe amplification and array comparative genomic hybridization analyses for prenatal diagnosis of cytogenomic abnormalities.
- Author
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Zhiyong Xu, Qian Geng, Fuwei Luo, Fang Xu, Peining Li, and Jiansheng Xie
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PRENATAL diagnosis ,COMPARATIVE genomic hybridization ,LIGATURE (Surgery) ,GENE amplification - Abstract
Background: The aims of this study were to evaluate the clinical utility of multiplex ligation-dependent probe amplification (MLPA) and array comparative genomic hybridization (aCGH) analyses on prenatal cases and to review prenatal ultrasound findings of cytogenomic syndromes. Results: Of the 54 prenatal cases analyzed, cytogenomic abnormalities were characterized in 14 cases. In four fetuses with abnormal ultrasound findings, a 40.701 Mb duplication of 8q22.3-q24.3 and a 23.839 Mb deletion of 7q33-q36.3 derived from a paternal balanced translocation, a de novo 13.062 Mb deletion of 11q24.1-q25 for Jacobsen syndrome, a de novo 19.971 Mb deletion of 7q11.23-q21.3 for type 1 split-hand/foot malformation (SHFM1), and a de novo 28.909 Mb duplication of 3q21.1-q25.1 were detected. A 699.8 Kb deletion at 5p15.33 for Cri du Chat syndrome was confirmed in a fetus with abnormal MLPA result. A fetus with abnormal maternal screening was detected with a de novo distal 1.747 Mb duplication at 2q37.1-q37.2 and a 6.664 Mb deletion at 2q37.2-q37.3. Of the eight cases referred by history of spontaneous abortions, derivative chromosomes 11 from paternal carriers of a balanced 8q/11q and a 10q/11q translocation were noted in two cases, simple aneuploids of trisomy 2 and trisomy 21 were seen in three cases, and compound aneuploids of two or three chromosomes were found in three cases. Post-test genetic counseling was performed with detailed genomic information and well characterized postnatal syndromic features. Conclusions: These results demonstrated that coupling MLPA screening and aCGH analysis are a costeffective approach to detect cytogenomic abnormalities in a prenatal setting. The aCGH analysis provided not only genomic maps of breakpoints and gene content of imbalanced regions but also better inference of related phenotypes for genetic counseling. Prenatal ultrasound findings reported in the literature for Jacobsen syndrome, SHFM and Cri du Chat syndrome were summarized for use as diagnostic references. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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37. Combined epithelial-mesenchymal transition with cancer stem cell-like marker as predictors of recurrence after radical resection for gastric cancer.
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Gui-fang Xu, Wei-jie Zhang, Qi Sun, Xinyun Xu, Xiaoping Zou, and Wenxian Guan
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STOMACH cancer treatment , *EPITHELIAL cells , *MESENCHYMAL stem cells , *BIOMARKERS , *SURGICAL excision - Abstract
Background The aim of the study was to identify the incidence and the predictors of recurrence after curative resection and the clinical significance of epithelial-mesenchymal transition (EMT) and stem cell-like phenotypes in gastric cancer. Methods In a total of 1,463 patients that underwent curative resection for gastric cancer between January 2001 and January 2008 at Drum Tower Hospital, 402 (27.5%) experienced recurrence. They were divided into early recurrence (within two years) and late recurrence (more than two years). The clinicopathological characteristics, including five EMT-related proteins (Snail-1, ZEB-1, E-cadherin, vimentin, and ß-catenin) and the gastric cancer stem cell markers CD44 and CD54, therapeutic modalities, survival time after recurrence, and recurrence patterns were compared between the two groups. Results Loss of E-cadherin expression and aberrant expression of vimentin and the known gastric cancer stem cell maker CD44 were significantly associated with aggressive clinicopathologic features. Multivariate analysis showed that stage III gastric cancer patients with early recurrence had larger tumors and more lymph node metastasis, coupled with aberrant expression EMT and cancer stem cell marker, than patients with late recurrence. Early recurrence was associated with more distant metastasis than late recurrence and patients tended to die within two years of recurrence. Conclusions Combined EMT with cancer stem cell-like marker is a predictor of recurrence after radical resection for gastric cancer. Advanced TNM stage was associated with early cancer death after recurrence. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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38. The first patient with a pure 1p36 microtriplication associated with severe clinical phenotypes.
- Author
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Fang Xu, Ya-Nan Zhang, De-Hua Cheng, Ke Tan, Chang-Gao Zhong, Guang-Xiu Lu, Ge Lin, and Yue-Qiu Tan
- Subjects
- *
PHENOTYPES , *DNA copy number variations , *MEDICAL genetics , *KARYOTYPES , *GENETIC polymorphisms - Abstract
Background Copy Number Variants (CNVs) is a new molecular frontier in clinical genetics. CNVs in 1p36 are usually pathogenic and have attracted the attention of cytogeneticists worldwide. None of 1p36 triplication has been reported thus far. Results We present three patients with CNVs in 1p36. Among them one is the first 1p36 tetrasomy due to a pure microtriplication and the other two are 1p36 microdeletion. Traditional chromosome G-banding technique showed a normal karyotype. Single nucleotide polymorphism (SNP) microarray analysis combined with multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH) were used to identify and confirm the chromosome microdeletion/microtriplication. The facial dysmorphisms of the patient with 1p36 tetrasomy differed from those two patients with 1p36 monosomy. The expression levels of B3GALT6, MIB2, PEX10 and PANK4 in the blood were determined, and differential expressions were observed between the patients and controls. Conclusions Our study shows the first case of 1p36 tetrasomy due to a pure microtriplication in a patient with severe intellectual disability and seizures. The study provides a new resource for studying the mechanisms of microtriplication formation, and provides an evidence that overexpression of the specific genes might be related the specific phenotype of 1p36 microtriplication. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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39. Role of Trichoderma reesei mitogen-activated protein kinases (MAPKs) in cellulase formation.
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Wang M, Zhang M, Li L, Dong Y, Jiang Y, Liu K, Zhang R, Jiang B, Niu K, and Fang X
- Abstract
Background: Despite being the most important cellulase producer, the cellulase-regulating carbon source signal transduction processes in Trichoderma reesei are largely unknown. Elucidating these processes is the key for unveiling how external carbon sources regulate cellulase formation, and ultimately for the improvement of cellulase production and biofuel production from lignocellulose., Results: In this work, the role of the mitogen-activated protein kinase (MAPK) signal transduction pathways on cellulase formation was investigated. The deletion of yeast FUS3 -like tmk1 in T. reesei leads to improved growth and significantly improved cellulase formation. However, tmk1 deletion has no effect on the transcription of cellulase-coding genes. The involvement of the cell wall integrity maintenance governing yeast Slt2-like Tmk2 in cellulase formation was investigated by overexpressing tmk3 in T. reesei Δtmk2 to restore cell wall integrity. Transcriptional analysis found little changes in cellulase-coding genes between T. reesei parent, Δtmk2 , and Δtmk2::OEtmk3 strains. Cell wall integrity decreased in T. reesei Δtmk2 over the parent strain and restored in Δtmk2::OEtmk3 . Meanwhile, cellulase formation is increased in T. reesei Δtmk2 and then decreased in T. reesei Δtmk2::OEtmk3., Conclusions: These investigations elucidate the role of Tmk1 and Tmk2 on cellulase formation: they repress cellulase formation, respectively, by repressing growth and maintaining cell wall integrity, while neither MAPK regulates the transcription of cellulase-coding genes. This work, together with the previous investigations, suggests that all MAPKs are involved in cellulase formation, while Tmk3 is the only MAPK involved in signal transduction for the regulation of cellulase expression on the transcriptional level.
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- 2017
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40. An environmentally friendly and productive process for bioethanol production from potato waste.
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Wang F, Jiang Y, Guo W, Niu K, Zhang R, Hou S, Wang M, Yi Y, Zhu C, Jia C, and Fang X
- Abstract
Background: China is the largest sweet potato producer and exporter in the world. Sweet potato residues (SPRs) separated after extracting starch account for more than 10 % of the total dry matter of sweet potatoes. In China, more than 2 million tons of SPRs cannot be utilized, and the unutilized SPRs are perishable and result in environmental pollution. Thus, an environmentally friendly and highly efficient process for bioethanol production from SPRs should be developed., Results: The swelling behaviour of cellulose causes high-gravity sweet potato residues to be recalcitrant to enzymatic hydrolysis. Cellulase plays a major role in viscosity reduction and glucose production. In contrast, pectinase has a minor role in viscosity reduction but acts as a "helper protein" to assist cellulase in liberating glucose, especially at low cellulase activity levels. In total, 153.46 and 168.13 g/L glucose were produced from high-gravity SPRs with cellulase and a mixture of cellulase and pectinase, respectively. These hydrolysates were fermented to form 73.37 and 79.00 g/L ethanol, respectively. Each kilogram of dry SPR was converted to form 209.62 and 225.71 g of ethanol, respectively., Conclusion: The processes described in this study have an enormous potential for industrial production of bioethanol because they are environmentally friendly, highly productive, economic with low cost, and can be easily manipulated.
- Published
- 2016
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