1. A simple and effective coumarin-based fluorescent probe for cysteine.
- Author
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Dai, Xi, Wu, Qing-Hua, Wang, Peng-Chong, Tian, Jie, Xu, Yu, Wang, Sheng-Qing, Miao, Jun-Ying, and Zhao, Bao-Xiang
- Subjects
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COUMARINS , *FLUORESCENT probes , *CYSTEINE , *ACRYLIC acid , *CHEMICAL synthesis , *RING formation (Chemistry) - Abstract
Abstract: Acrylic acid 3-acetyl-2-oxo-2 H-chromen-7-yl ester (ACA) was rationally designed and synthesized as a simple and effective fluorescent probe for sensing cysteine with high selectivity and naked-eye detection. The probe can detect cysteine by fluorescence spectrometry with a detection limit of 0.657μM and can be used with calf serum and in live cell imaging. The conjugate addition/cyclization sequence mechanism of the reaction between ACA and cysteine was confirmed by ESI-MS and fluorescence spectra. [Copyright &y& Elsevier]
- Published
- 2014
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