1. Aberrant Peripheral Immune Function in a Good Syndrome Patient.
- Author
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Chen, Xian, Zhang, Jie-xin, Shang, Wen-wen, Xie, Wei-ping, Jin, Shu-xian, and Wang, Fang
- Subjects
RESPIRATORY infections ,CYTOKINES ,THYMOMA ,THYMECTOMY ,INTERLEUKIN-17 ,ANTIGENS ,B cells ,CELL culture ,CELL physiology ,CELL receptors ,CELL separation ,DIARRHEA ,FLOW cytometry ,IMMUNOLOGICAL deficiency syndromes ,INTERFERONS ,INTERLEUKINS ,T cells ,THYMUS tumors - Abstract
Good's syndrome (GS) is often accompanied by recurrent respiratory infections and chronic diarrhea. The main purpose was to evaluate the peripheral immune status of a GS patient after thymoma resection. Twenty healthy volunteers were recruited as healthy controls (HCs). Flow cytometry was applied to determine the proportions of circuiting CD4+ T cells, CD8+ T cells, γδT cells, and regulatory T (Treg) cells in our GS patient. We also examined the proliferation capability of ex vivo CD4+ T cells and detected the levels of cytokines interferon- (IFN-) γ and interleukin-17A secreted by ex vivo immune cells from this GS patient. Compared with healthy control subjects, this GS patient had fewer B cells, an inverted ratio of CD4+/CD8+ cells, and more Treg cells in his peripheral blood. Additionally, the patient's Vδ2 T cell levels were significantly decreased despite having a normal percentage of γδT cells. Ex vivo peripheral CD4+ T cells from the patient showed insufficient proliferation and division potential as well as excessive expression of PD-1. Moreover, IFN-γ was predominantly derived from CD8+ T cells in this GS patient, rather than from CD4+ T cells and γδT cells. This GS patient had impaired T and B cell immunological alternations and cytokine disruptions after thymectomy. Detailed research should focus on therapies that can adjust the immune status in such patients for a better outcome. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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