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In the three-phase traffic flow studies, the traffic flow characteristic at the bottleneck section is a hot spot in the academic field. The controversy about the characteristics of the synchronized flow at bottleneck is also the main contradiction between the three-phase traffic flow theory and the traditional traffic flow theory. Under the framework of three-phase traffic flow theory, this paper takes the on-ramp as an example to discuss the traffic flow characteristics at the bottleneck section. In particular, this paper mainly conducts the micro-analysis to the effect of lane change under the two lane conditions, as well as the effect of the on-ramp on the main line traffic flow. It is found that when the main road flow is low, the greater the on-ramp inflow rate, the higher the average speed of the whole road section. As the probability of vehicles entering from the on-ramp increases, the flow and the average speed of the main road are gradually stabilized, and then the on-ramp inflow vehicles no longer have a significant impact on the traffic flow. In addition, this paper focuses on the velocity disturbance generated at the on-ramp, and proposes the corresponding on-ramp control strategy based on it, and the simulation verified that the control strategy can reasonably control the traffic flow by the on-ramp, which can meet the control strategy requirements to some extent. [ABSTRACT FROM AUTHOR]

Dispersed fringe sensor (DFS) is an important phasing sensor of next-generation optical astronomical telescopes. The measurement errors induced by the measurement noise of three piston estimation methods for the DFS including least-squared fitting (LSF) method, frequency peak location (FPL) method and main peak position (MPP) method, are analyzed theoretically and validated experimentally in this paper. The experimental results coincide well with the theoretical analyses. The MPP, FPL, LSF are used respectively when the DFS operates with broadband light (central wavelength: 706 nm, bandwidth: 23 nm). The corresponding root mean square (RMS) value of estimated piston error can be achieved to be 1 nm, 3 nm, 26 nm, respectively. Additionally, the range of DFS with the FPL can be more than 100 μm at the same time. The FPL method can work well both in coarse and fine phasing stages with acceptable accuracy, compared with LSF method and MPP method. [ABSTRACT FROM AUTHOR]

Convenient and simultaneous detection of multiple biomarkers such as DNA and proteins with biocompatible materials and good analytical performance still remains a challenge. Herein, we report the respective and simultaneous detection of DNA and bovine α-thrombin (thrombin) entirely based on biocompatible carbon materials through a specially designed fluorescence on-off-on process. Colorful fluorescence, high emission efficiency, good photostability and excellent compatibility enables graphene quantum dots (GQDs) as the best choice for fluorophores in bioprobes, and thus two-colored GQDs as labeling fluorophores were chemically bonded with specific oligonucleotide sequence and aptamer to prepare two probes targeting the DNA and thrombin, respectively. Each probe can be assembled on the graphene oxide (GO) platform spontaneously by π–π stacking and electrostatic attraction; as a result, fast electron transfer in the assembly efficiently quenches the fluorescence of probe. The presence of DNA or thrombin can trigger the self-recognition between capturing a nucleotide sequence and its target DNA or between thrombin and its aptamer due to their specific hybridization and duplex DNA structures or the formation of apatamer–substrate complex, which is taken advantage of in order to achieve a separate quantitative analysis of DNA and thrombin. A dual-functional biosensor for simultaneous detection of DNA and thrombin was also constructed by self-assembly of two probes with distinct colors and GO platform, and was further evaluated with the presence of various concentrations of DNA and thrombin. Both biosensors serving as a general detection model for multiple species exhibit outstanding analytical performance, and are expected to be applied in vivo because of the excellent biocompatibility of their used materials. [ABSTRACT FROM AUTHOR]

The transport behavior of pressure-driven aqueous electrolyte solution through charged carbon nanotubes (CNTs) is studied by using molecular dynamics simulations. The results reveal that the presence of charges around the nanotube can remarkably reduce the flow velocity as well as the slip length of the aqueous solution, and the decreasing of magnitude depends on the number of surface charges and distribution. With 1-M KCl solution inside the carbon nanotube, the slip length decreases from 110 nm to only 14 nm when the number of surface charges increases from 0 to 12 e. This phenomenon is attributed to the increase of the solid–liquid friction force due to the electrostatic interaction between the charges and the electrolyte particles, which can impede the transports of water molecules and electrolyte ions. With the simulation results, we estimate the energy conversion efficiency of nanofluidic battery based on CNTs, and find that the highest efficiency is only around 30% but not 60% as expected in previous work. [ABSTRACT FROM AUTHOR]