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The scroll paintings for ancestor trees have been used to inherit the spirit of ancestor worship as a historical record of family development since the late Ming Dynasty in China. A severely degraded scroll painting of an ancestor tree (made of cotton textiles) needs intervention and conservation treatment to mitigate further deterioration. On the basis of the previously reported characterization results for the painting, in this paper, a suspension that is composed of 0.6% cellulose nanofibril (CNF) and nanosized 0.15% MgO in aqueous solvent (denoted as the CNF-MgO susairpension) was prepared. Conventional characterization methods were used to assess the properties of model samples before and after treatment with the CNF-MgO suspension, as well as before and after degradation under two sets of conditions. The results show that the treated model samples are slightly alkaline, given the deposit of alkaline particles, and demonstrate good mechanical properties before and after degradation due to the increase in fiber-to-fiber bond and mitigation of acid-catalyzed hydrolysis. In spite of the non-transparency of CNF and MgO nanoparticles, they have little impact on the optical properties of textiles, as verified by transmittance data and the determination of color changes. This suspension was then used to reinforce and restore the scroll painting in a practical conservation process. The application of CNF and MgO nanoparticles on textile objects investigated in this study would expand our understanding of the conservation of such objects, especially for those that have already become acidic and degraded. [ABSTRACT FROM AUTHOR]

CoCrCuFeMnNix (x = 0, 0.5, 1.0, 1.5, 2.0 mol, named as Ni0, Ni0.5, Ni1.0, Ni1.5, and Ni2.0, respectively) high-entropy alloy powders (HEAPs) were prepared via mechanical alloying (MA), and XRD, SEM, EDS, and vacuum annealing were used to study the alloying behavior, phase transition, and thermal stability. The results indicated that the Ni0, Ni0.5, and Ni1.0 HEAPs were alloyed at the initial stage (5–15 h), the metastable BCC + FCC two-phase solid solution structure was formed, and the BCC phase disappeared gradually with the prolonging of ball milling time. Finally, a single FCC structure was formed. Both Ni1.5 and Ni2.0 alloys with high nickel content formed a single FCC structure during the whole mechanical alloying process. The five kinds of HEAPs showed equiaxed particles in dry milling, and the particle size increased with an increase in milling time. After wet milling, they changed into lamellar morphology with thickness less than 1 μm and maximum size less than 20 μm. The composition of each component was close to its nominal composition, and the alloying sequence during ball milling was Cu→Mn→Co→Ni→Fe→Cr. After vacuum annealing at 700~900 °C, the FCC phase in the HEAPs with low Ni content transformed into FCC2 secondary phase, FCC1 primary phase, and a minor σ phase. The thermal stability of HEAPs can be improved by increasing Ni content. [ABSTRACT FROM AUTHOR]

The worldwide spread of COVID-19 continues to impact our lives and has led to unprecedented damage to global health and the economy. This highlights the need for an efficient approach to rapidly develop therapeutics and prophylactics against SARS-CoV-2. We modified a single-domain antibody, SARS-CoV-2 VHH, to the surface of the liposomes. These immunoliposomes demonstrated a good neutralizing ability, but could also carry therapeutic compounds. Furthermore, we used the 2019-nCoV RBD-SD1 protein as an antigen with Lip/cGAMP as the adjuvant to immunize mice. Lip/cGAMP enhanced the immunity well. It was demonstrated that the combination of RBD-SD1 and Lip/cGAMP was an effective preventive vaccine. This work presented potent therapeutic anti-SARS-CoV-2 drugs and an effective vaccine to prevent the spread of COVID-19. [ABSTRACT FROM AUTHOR]

The direct wave between the transceiver antenna negatively affects the dynamic range and imaging quality of ground penetrating radar (GPR). Suppressing this direct wave is a vital problem in enhancing the performance of the whole GPR system. In this paper, a Minkowski-fractal metamaterial absorber (MMA) with the resistive film is proposed in the GPR transceiver antenna to reduce the mutual coupling. The simulated and measured results indicate that this MMA has an effective wideband absorption in 1.0-8.0 GHz. And the thickness of MMA is only 0.007 λ 0 (with respect to 2.0 GHz). This wideband MMA can reduce the mutual coupling of the proposed GPR transceiver antenna by an average of 10 dB. And it also mitigates the time-domain ringing problem of the transmit antenna. Real-world experiments demonstrate that the direct wave from the transmitting antenna can be reduced and the target echo arriving at the receiving antenna can be increased if this MMA is placed in the proposed transceiver antenna. This resistive film-based MMA offers great promise in realizing low-cost, compact, and lightweight GPR antennas, which can also be extended to high-frequency microwave imaging. [ABSTRACT FROM AUTHOR]

The fragile paper is treated to improve the stability and appearance of the paper artifact, such as washing, lining, deacidification, and reinforcement. During the above treatments, paper documents inevitably make contact with water directly, leading to the appearance change, stability decrease, and migration or fading of anionic water-sensitive dyes, which are seriously harmful to information security. Herein, Hydroxypropyltrimethyl ammonium chloride chitosan (HACC) nanoparticles were employed for the reinforcement and concomitant inhibition of anionic water-sensitive dye migration on fragile paper. HACC nanoparticles were prepared through physical ball grinding method and characterized via LPSA, SEM, TEM, XRD and FTIR. To evaluate the protective potential of HACC nanoparticles coating, the chemical and mechanical properties of coated and uncoated papers were evaluated after dry heat and hygrothermal accelerated aging. Additionally, good color stability of anionic water-sensitive dyes was observed on the paper coated with HACC nanoparticles after lining technology. Finally, the interaction mechanism between the anionic water-sensitive dyes and HACC nanoparticles was analyzed using an ultraviolet spectrophotometer and FTIR. The as-proposed technique can provide technical support to improve the mechanical properties of fragile paper and enhance the anionic water-sensitive dyes stability in the aqueous phase. [ABSTRACT FROM AUTHOR]

The cyclic guanosine monophosphate–adenosine monophosphate synthase–stimulator of interferon genes–TANK-binding kinase 1–interferon regulating factor 3 (cGAS-STING-TBK1-IRF3) axis is now acknowledged as the major signaling pathway in innate immune responses. However, 2′,3′-cGAMP as a STING stimulator is easily recognized and degraded by ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which reduces the effect of tumor immunotherapy and promotes metastatic progression. In this investigation, the structure-based virtual screening strategy was adopted to discover eight candidate compounds containing zinc-binding quinazolin-4(3H)-one scaffold as ENPP1 inhibitors. Subsequently, these novel inhibitors targeting ENPP1 were synthesized and characterized by NMR and high-resolution mass spectra (HRMS). In bioassays, 7-fluoro-2-(((5-methoxy-1H-imidazo[4,5-b]pyridin-2-yl)thio)methyl)quina-zolin-4(3H)-one(compound 4e) showed excellent activity against the ENPP1 at the molecular and cellular levels, with IC50 values of 0.188 μM and 0.732 μM, respectively. Additionally, compound 4e had superior selectivity towards metastatic breast cancer cells (4T1) than towards normal cells (LO2 and 293T) in comparison with cisplatin, indicating that compound 4e can potentially be used in metastatic breast cancer therapy. On the other hand, compound 4e upgraded the expression levels of IFN-β in vivo by preventing the ENPP1 from hydrolyzing the cGAMP to stimulate a more potent innate immune response. Therefore, this compound might be applied to boost antitumor immunity for cancer immunotherapy. Overall, our work provides a strategy for the development of a promising drug candidate targeting ENPP1 for tumor immunotherapy. [ABSTRACT FROM AUTHOR]

The synchronous genetic regulatory networks model includes the drive system and response system, and the drive-response system is symmetric. From a biological point of view, this model illustrates the dynamic behaviors in gene-to-protein processes, in terms of transcription and translation. This paper introduces a model of genetic regulatory networks with time delay. The fixed-time synchronization control problem of the proposed model is studied based on fixed-time stability theory and the Lyapunov method. Concretely, the authors first propose a way to switch from the given model to matrix form. Then, two types of novel controllers are designed and the corresponding sufficient conditions are investigated respectively to ensure the fixed-time synchronization goal. Moreover, the settling times of fixed-time synchronization are estimated for the addressed discontinuous controllers, which are not dependent on the initial or delayed states of the model. Finally, numerical simulations are presented and compared to illustrate the benefits of the theoretical results. [ABSTRACT FROM AUTHOR]

The effects of ultrasonic-aided quenching (USQ) on the microstructure and corrosion properties of 35CrMoV steel in a simulated seawater environment (wt % (NaCl) = 3.5) were studied. The corrosion properties of the steel were studied by electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization curves. The scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) were used to characterize the microstructure and corrosion products. Combined with the EIS equivalent circuit, the corrosion mechanism was investigated. The results show that the microstructure of the USQ specimen is small martensite and that there was no residual ferrite compared with an un-ultrasonic-aided quenching (UUSQ) sample. It was also found that the corrosion products of USQ sample are less than the UUSQ sample, and the corrosion cracks are lighter and thinner than that of the UUSQ sample, which indicates that the corrosion resistance of 35CrMoV in a seawater environment has been obviously improved. [ABSTRACT FROM AUTHOR]

Self-assembled submicron nickel particles were successfully synthesized via the one-step surfactant-assisted solvothermal method. The impact of surfactant and reducing agent stoichiometry is investigated in this manuscript. Different morphologies and structures of Ni particles, including flower-like nanoflakes, hydrangea-like structures, chain structures, sphere-like structures, and hollow structures were prepared through different processing conditions with two parameters such as temperature and time. Based on scanning electron microscopy (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA) and vibrating sample magnetometry (VSM), the submicron nickel particles show good saturation magnetization and excellent thermal stabilities with a possible growth mechanism for the variety of the structure-tuned formation. Importantly, the microwave absorption properties of the submicron nickel particles were studied. The lowest reflection loss of Ni-P9/T200/H15 with a thin layer thickness of 1.7 mm can reach -42.6 dB at 17.3 GHz. [ABSTRACT FROM AUTHOR]

The Nid site coordination microenvironment of a truncated acetyl-coenzyme A synthase has been designed systematically for functional conversion to a Ni-SOD-like enzyme. To this end, the first strategy is to introduce an axial histidine ligand, using mutations F598H, S594H and S594H-GP individually. The resulting three mutants obtained Ni-SOD-like activity successfully, although the catalytic activity was about 10-fold lower than in native Ni-SOD. The second strategy is to mimic the H-bond network in the second sphere coordination microenvironment of the native Ni-SOD. Two mutations based on F598H (EFG-F598H and YGP-F598H) were designed. The successful EFG-F598H exhibited ~3-fold Ni-SOD-like activity of F598H. These designed Ni-SOD-like metalloproteins were characterized by UV/Vis, EPR and Cyclic voltammetry while F598H was also characterized by X-ray protein crystallography. The pH titrations were performed to reveal the source of the two protons required for forming H2O2 in the SOD catalytic reaction. Based on all of the results, a proposed catalytic mechanism for the Ni-SOD-like metalloproteins is presented. [ABSTRACT FROM AUTHOR]

Hot compression tests were performed with strain rates (0.01–10 s−1) and temperatures (850–1150 °C). The power law relationship between the critical stress and critical strain and Zener–Hollomon parameters was determined by θ-σ curves. Microstructure was investigated by electron backscattered diffraction. The results showed that the flow behavior and microstructure of 35CrMo steel was affected by ultrasonic-assisted casting. The activation energy of non-ultrasonic and ultrasonic-assisted 35CrMo steel were 410 ± 9.9 and 386 ± 9.4 kJ/mol, respectively, and the activation energy of ultrasonic-assisted specimens was reduced by 6%. In addition, the ultrasonic-assisted treatment refines the grains to some extent and makes the softening process of ultrasonic-assisted samples progress faster, which promoted the development of dynamic recrystallization and the production of Σ3 boundaries. The discontinuous dynamic recrystallization was the main DRX nucleation mechanism of the 35CrMo steel. [ABSTRACT FROM AUTHOR]

Paper acidification causes paper relics to undergo embrittlement and decay, to form dregs, and even to break upon a single touch; therefore, reinforcement and deacidification treatments are essential steps for paper conservation and to retard the deterioration and prolong the life of objects. Polymeric adhesives play an essential role in reinforcement and deacidification treatments, although it is not well studied. In this work, the effect of polymeric adhesives on the conservation process and their protective effects on acidified paper relics were studied. Firstly, three polymeric adhesives, including wheat starch paste, polyvinyl butyral (PVB), and polyvinyl alcohol (PVA), were selected as research objects. Subsequently, their effects on four popular conservation methods were further discussed, including traditional mounting, hot-melt with silk net, alcohol-soluble cotton mesh, and water-soluble cotton mesh. Additionally, as an example, the reversibility and long-term durability of water-soluble adhesive PVA-217 were assessed. Using a computer measured and controlled folding endurance tester, pendulum tensile strength tester, tear tester, burst tester, FT-IR, video optical contact angle tester, and other instruments, the conservation application of water-soluble adhesives in paper relics was evaluated. This study provides a scientific basis and experimental data for the application of polymeric adhesives in the conservation of paper relics. [ABSTRACT FROM AUTHOR]

Deacidification plays an important role in the conservation of paper-based cultural heritage objects. Herein, a novel approach for the conservation of scale paper-based cultural heritage objects is proposed using a mixture of argon and ethylene oxide (EO-Ar) for the first time. The optimum process conditions for deacidification of ethylene oxide and argon mixture system are determined by orthogonal testing. To evaluate the stabilization effect of paper treated with EO-Ar, the degradation of the mechanical properties (tensile strength, folding endurance and tearing strength tests) of paper after artificial aging was evaluated. The results show that the treated paper had better durability with respect to tensile strength, folding endurance and tearing strength. Additionally, thermal stability, crystallinity and fiber wall thickness increased after EO-Ar treated, which was determined by scanning electron microscope (SEM), diffraction of X-rays (XRD), and thermo gravimetric (TG) analysis. Some compounds, such as polyethylene glycol, organic acids, esters, were detected by GC-MS after treatment with EO-Ar. Two hundred and forty books including acidic, weak acidic and alkaline books were successfully deacidified, resulting in pH values of paper ranges suitable for paper preservation. Finally, a possible mechanism of deacidification of EO-Ar was proposed. [ABSTRACT FROM AUTHOR]

The effects of ultrasonic introduced by L-shaped sonotrodes made of high-temperature-resistant ceramic on the microstructure and macro-segregation of solidifying 15t AA2219 aluminum alloy ingots have been examined in the present study. The macroscopic morphology of the corrosion of the sonotrode has been observed. Grain refinement has been observed, the shape and size of the precipitated phase of the ingot were counted, and the degree of segregation along the transverse direction at 500 mm from the head of the ingot has been evaluated. The results reveal that the L-shaped ceramic ultrasonic introduction device can effectively avoid the erosion of high-temperature melt on the sonotrode and the heat radiation of the high-temperature heat flow to the transducer. Furthermore, the scanning electron microscope (SEM) and chemical composition detection results also indicate that the inter-dendritic micro-segregation of the equiaxed grains can be reduced, and the macro-segregation of the chemical composition of the ingot can be suppressed, and more homogeneous microstructures can be obtained when ultrasonic has been applied during solidification. [ABSTRACT FROM AUTHOR]

The effect of deep cryogenic treatment on microstructure and wear resistance of LC3530 Fe-based powder laser cladding coating was investigated in this paper. The cladding coating was subjected to deep cryogenic treatment for the different holding times of 3, 6, 9, 12, and 24 h, followed by tempering at room temperature. Microstructure of the cladding coating was observed by optical microscope (OM) and the microhardness was measured by the Vickers-hardness tester. The wear was tested by ball and flat surface grinding testing conducted on the material surface comprehensive performance tester. The wear scars were analyzed using a non-contact optical surface profiler and scanning electron microscope (SEM). The results showed the grain size of cladding coating after 12 h of deep cryogenic treatment was significantly reduced by 36.50% compared to the non-cryogenically treated cladding coating, and the microhardness value increased by approximately 34%. According to the wear coefficient calculated by the Archard model, the wear resistance improved about five times and the wear mechanism was micro-ploughing. The deep cryogenic treatment could enhance the wear resistance of the cladding coating by forming a wear resistant alloy compound and higher surface microhardness. [ABSTRACT FROM AUTHOR]

The double-ring infiltrometer is widely used to measure soil unsaturated hydraulic conductivity in the field. The scale effect of the inner and outer ring size (especially the inner one) affects the measurement results. In the semi-arid steppe, where water is scarce and transportation is inadequate, studying the scale effect caused by the inner-ring diameter of the infiltrometer can reduce the test consumption on the premise of ensuring the test accuracy. In this paper, a total of 190 double-ring infiltration tests with different inner-ring diameters (15, 20, 25, 30, and 40 cm) and 0.33 times outer buffer index were carried out at 38 sites with five soil types in the Xilin river basin, China. Results showed that: (1) When comparing the simulated parameters of six infiltration models, parameters increased with the increase of the infiltrometer inner diameter, but the trend gradually slowed down, indicating that the increase of the infiltrometer inner diameter would weaken the influence of the infiltrometer scale effect. However, the infiltrometer with an inner diameter of 40 cm is not enough to completely overcome the scale effect. (2) Through principal component analysis, the infiltration process is mainly affected by the particle size and the initial moisture content. (3) The soil infiltration map based on infiltration tests was more practical than the soil type map, which can provide a theoretical basis for ecological and soil restoration in the future. [ABSTRACT FROM AUTHOR]

Hot deformation experiments of as-cast 35CrMoV steel, with strain rates of 0.01 s−1 and 10 s−1, deformation temperatures of 850, 950, and 1050 °C, and an extreme deformation reaching 50%, were carried out using a Gleeble-3810 thermal simulator. Electrochemical corrosion experiments were conducted on the deformed specimens. The microstructure was observed by optical microscope (OM), and the corrosion morphology and corrosion products of the specimens were investigated by scanning electron microscopy (SEM), energy disperse spectroscopy (EDS), confocal laser scanning microscopy (CLSM), and X-ray diffraction (XRD) techniques. The results show that the grain size increased gradually with an increase in the deformation temperature at the same strain rate, whereas the corrosion resistance deteriorated. At the same deformation temperature, the grain size becomes smaller as the strain rate increases, which enhances the corrosion resistance. This is mainly attributed to the fine grains, which can form more grain boundaries, increase the grain boundary area, and accelerate the formation of the inner rust layer at the beginning of corrosion. Moreover, fine grains can also refine the rust particles and enhance the bonding strength between the inner rust layer and the matrix. The denseness and stability of the inner rust layer increases as the corrosion process progresses, thereby improving corrosion resistance. [ABSTRACT FROM AUTHOR]

Wear resistance of metallic materials can be effectively improved by the deep cryogenic treatment. In this study, different deep cryogenic treatment conditions were considered, with different soaking durations between quenching and tempering. The main objective is investigating the effects of deep cryogenic treatment and exploring the relationship between the mechanical properties and the microstructure of GB 35CrMoV steel. Hardness and relative wear ratios of samples were evaluated by the Vickers-hardness test and the pin-on-disk wear test, respectively. Worn surface was characterized by a non-contact optical surface profiler. Microstructures were studied by scanning electron microscope (SEM) and X-ray diffraction (XRD). Significant improvements in hardness and wear resistance are observed for higher cryogenic soaking times; the root mean square deviation (RMS) parameter (Sq) was employed to evaluate the effect of deep cryogenic treatment on the worn surface roughness; the improvements were ascribed to the precipitated carbides. The mechanism can be interpreted not only as the promoted effect of deep cryogenic treatment in the decomposition kinetics of martensite, but also as the acceleration on the Ostwald ripening process. [ABSTRACT FROM AUTHOR]

Hot compression tests were performed with strain rates (0.01-10 s -1 ) and temperatures (850-1150 °C). The power law relationship between the critical stress and critical strain and Zener-Hollomon parameters was determined by θ - σ curves. Microstructure was investigated by electron backscattered diffraction. The results showed that the flow behavior and microstructure of 35CrMo steel was affected by ultrasonic-assisted casting. The activation energy of non-ultrasonic and ultrasonic-assisted 35CrMo steel were 410 ± 9.9 and 386 ± 9.4 kJ/mol, respectively, and the activation energy of ultrasonic-assisted specimens was reduced by 6%. In addition, the ultrasonic-assisted treatment refines the grains to some extent and makes the softening process of ultrasonic-assisted samples progress faster, which promoted the development of dynamic recrystallization and the production of Σ 3 boundaries. The discontinuous dynamic recrystallization was the main DRX nucleation mechanism of the 35CrMo steel.

Inhibition of β-lactamases presents a promising strategy to restore the β-lactams antibacterial activity to resistant bacteria. In this work, we found that aromatic carboxyl substituted 2-triazolylthioacetamides 1a - j inhibited VIM-2, exhibiting an IC 50 value in the range of 20.6-58.6 μM. The structure-activity relationship study revealed that replacing the aliphatic carboxylic acid with aromatic carboxyl improved the inhibitory activity of 2-triazolylthioacetamides against VIM-2. 1a - j (16 mg/mL) restored the antibacterial activity of cefazolin against E. coli cell expressing VIM-2, resulting in a 4-8-fold reduction in MICs. The isothermal titration calorimetry (ITC) characterization suggested that the primary binding 2-triazolylthioacetamide ( 1b , 1c , or 1h ) to VIM-2 was a combination of entropy and enthalpy contributions. Further, the crystal structure of VIM-2 in complex with 1b was obtained by co-crystallization with a hanging-drop vapour-diffusion method. The crystal structure analysis revealed that 1b bound to two Zn(II) ions of the enzyme active sites, formed H-bound with Asn233 and structure water molecule, and interacted with the hydrophobic pocket of enzyme activity center utilizing hydrophobic moieties; especially for the phenyl of aromatic carboxyl which formed π-π stacking with active residue His263. These studies confirmed that aromatic carboxyl substituted 2-triazolylthioacetamides are the potent VIM-2 inhibitors scaffold and provided help to further optimize 2-triazolylthioacetamides as VIM-2 even or broad-spectrum MβLs inhibitors.