1. Extraction-Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2.
- Author
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Carney, Patrick R., Duellman, Tyler, Chan, Jia-Yi, Wells, Lauren, Tessmer, Michael, Frater-Rubsam, Leah, Zeller, Molly, Field, Mark, Speers, James, Tyrrell, Kelly, Thompson, Luke, Bondurant, Michael, Morin, Tami, Dagnon, Tamra, Goff, Brian, Runde, Corissa, Splinter-Bondurant, Sandra, Konsitzke, Charles, Kelly, Patrick, and Bradfield, Christopher A.
- Subjects
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COVID-19 pandemic , *PUBLIC health , *SARS-CoV-2 , *REVERSE transcriptase polymerase chain reaction , *COVID-19 vaccines - Abstract
The COVID-19 pandemic necessitated sensitive, fast, and inexpensive testing for the virus in 2020 prior to the widespread availability of vaccines. Early testing efforts were limited by bottlenecks on reagents, low-throughput testing options, and the slow return of test results. In this paper, we detail the testing pipeline we established at the University of Wisconsin-Madison for rapid, inexpensive, and sensitive surveillance testing for SARS-CoV-2, and we highlight the strengths of the platform that would allow it to be applied to other disease surveillance projects, SARS-CoV-2 variant testing, or future pandemics. This pipeline can be quickly established for further accreditation and clinical application. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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