16 results on '"Ribeiro, Ana R."'
Search Results
2. Size-at-Age, Growth and Condition of a Trout (Salmo trutta L.) Population from Central Portugal
- Author
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Ribeiro, Ana R., primary, Silva, Sara, additional, Mateus, Catarina S., additional, Quintella, Bernardo R., additional, Almeida, Pedro R., additional, and Alexandre, Carlos M., additional
- Published
- 2022
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3. Per- and Poly-Fluoroalkyl Substances in Portuguese Rivers: Spatial-Temporal Monitoring.
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Barbosa, Marta O., Ratola, Nuno, Homem, Vera, Pereira, M. Fernando R., Silva, Adrián M. T., Ribeiro, Ana R. L., Llorca, Marta, and Farré, Marinella
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FLUOROALKYL compounds ,FOOD chains ,WATERSHEDS ,GREEN algae ,ENVIRONMENTAL risk - Abstract
Eighteen per-and polyfluoroalkyl substances (PFASs) were investigated in surface waters of four river basins in Portugal (Ave, Leça, Antuã, and Cértima) during the dry and wet seasons. All sampling sites showed contamination in at least one of the seasons. In the dry season, perfluorooctanoate acid (PFOA) and perfluoro-octane sulfonate (PFOS), were the most frequent PFASs, while during the wet season these were PFOA and perfluobutane-sulfonic acid (PFBS). Compounds detected at higher concentrations were PFOS (22.6 ng L
−1 ) and perfluoro-butanoic acid (PFBA) (22.6 ng L−1 ) in the dry and wet seasons, respectively. Moreover, the prospective environmental risks of PFASs, detected at higher concentrations, were evaluated based on the Risk Quotient (RQ) classification, which comprises acute and chronic toxicity. The results show that the RQ values of eight out of the nine PFASs were below 0.01, indicating low risk to organisms at different trophic levels in the four rivers in both seasons, wet and dry. Nevertheless, in the specific case of perfluoro-tetradecanoic acid (PFTeA), the RQ values calculated exceeded 1 for fish (96 h) and daphnids (48 h), indicating a high risk for these organisms. Furthermore, the RQ values were higher than 0.1, indicating a medium risk for fish, daphnids and green algae (96 h). [ABSTRACT FROM AUTHOR]- Published
- 2023
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4. Nanosafety: An Evolving Concept to Bring the Safest Possible Nanomaterials to Society and Environment.
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Lebre, Filipa, Chatterjee, Nivedita, Costa, Samantha, Fernández-de-Gortari, Eli, Lopes, Carla, Meneses, João, Ortiz, Luís, Ribeiro, Ana R., Vilas-Boas, Vânia, and Alfaro-Moreno, Ernesto
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NANOSTRUCTURED materials ,PRODUCT life cycle assessment ,WATER purification ,CARCINOGENS ,IMMUNOTOXICOLOGY ,NANOPARTICLE toxicity - Abstract
The use of nanomaterials has been increasing in recent times, and they are widely used in industries such as cosmetics, drugs, food, water treatment, and agriculture. The rapid development of new nanomaterials demands a set of approaches to evaluate the potential toxicity and risks related to them. In this regard, nanosafety has been using and adapting already existing methods (toxicological approach), but the unique characteristics of nanomaterials demand new approaches (nanotoxicology) to fully understand the potential toxicity, immunotoxicity, and (epi)genotoxicity. In addition, new technologies, such as organs-on-chips and sophisticated sensors, are under development and/or adaptation. All the information generated is used to develop new in silico approaches trying to predict the potential effects of newly developed materials. The overall evaluation of nanomaterials from their production to their final disposal chain is completed using the life cycle assessment (LCA), which is becoming an important element of nanosafety considering sustainability and environmental impact. In this review, we give an overview of all these elements of nanosafety. [ABSTRACT FROM AUTHOR]
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- 2022
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5. Nanomaterial Exposure, Extracellular Vesicle Biogenesis and Adverse Cellular Outcomes: A Scoping Review.
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Lima, Thais S. M., Souza, Wanderson, Geaquinto, Luths R. O., Sanches, Priscila L., Stepień, Ewa. L., Meneses, João, Fernández-de Gortari, Eli, Meisner-Kober, Nicole, Himly, Martin, Granjeiro, José M., and Ribeiro, Ana R.
- Abstract
The progressively increasing use of nanomaterials (NMs) has awakened issues related to nanosafety and its potential toxic effects on human health. Emerging studies suggest that NMs alter cell communication by reshaping and altering the secretion of extracellular vesicles (EVs), leading to dysfunction in recipient cells. However, there is limited understanding of how the physicochemical characteristics of NMs alter the EV content and their consequent physiological functions. Therefore, this review explored the relevance of EVs in the nanotoxicology field. The current state of the art on how EVs are modulated by NM exposure and the possible regulation and modulation of signaling pathways and physiological responses were assessed in detail. This review followed the manual for reviewers produced by The Joanna Brigs Institute for Scoping Reviews and the PRISMA extension for Scoping Reviews (PRISMA-ScR): checklist and explanation. The research question, "Do NMs modulate cellular responses mediated by EVs?" was analyzed following the PECO model (P (Population) = EVs, E (Exposure) = NMs, C (Comparator) = EVs without exposure to NMs, O (Outcome) = Cellular responses/change in EVs) to help methodologically assess the association between exposure and outcome. For each theme in the PECO acronym, keywords were defined, organized, and researched in PubMed, Science Direct, Scopus, Web of Science, EMBASE, and Cochrane databases, up to 30 September 2021. In vitro, in vivo, ex vivo, and clinical studies that analyzed the effect of NMs on EV biogenesis, cargo, and cellular responses were included in the analysis. The methodological quality assessment was conducted using the ToxRTool, ARRIVE guideline, Newcastle Ottawa and the EV-TRACK platform. The search in the referred databases identified 2944 articles. After applying the eligibility criteria and two-step screening, 18 articles were included in the final review. We observed that depending on the concentration and physicochemical characteristics, specific NMs promote a significant increase in EV secretion as well as changes in their cargo, especially regarding the expression of proteins and miRNAs, which, in turn, were involved in biological processes that included cell communication, angiogenesis, and activation of the immune response, etc. Although further studies are necessary, this work suggests that molecular investigations on EVs induced by NM exposure may become a potential tool for toxicological studies since they are widely accessible biomarkers that may form a bridge between NM exposure and the cellular response and pathological outcome. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Biodegradable wet-spun fibers as delivery platforms for the bactericidal effect of the natural-origin biomolecules, cinnamon, clove and cajeput essential oils
- Author
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Felgueiras, Helena P., primary, Homem, Natália C., additional, Teixeira, Marta A., additional, Teixeira, Marta O., additional, Ribeiro, Ana R. M., additional, Antunes, Joana C., additional, and Amorim, M. Teresa P., additional
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- 2020
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7. Activity of Wet-Spun Fibers Chemically Modified with Active Biomolecules against Gram-Positive and Gram-Negative Bacteria
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Felgueiras, Helena P., primary, Homem, Natália C., additional, Ribeiro, Ana R. M., additional, Teixeira, Marta O., additional, Teixeira, Marta A., additional, Antunes, Joana C., additional, and Amorim, Maria Teresa P., additional
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- 2020
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8. Bactericidal action of cinnamon, clove and cajeput oils loaded onto CA/PCL wet-spun fibers for a localized, controlled biomolecule delivery
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Felgueiras, Helena P., primary, Homem, Natália C., additional, Ribeiro, Ana R. M., additional, Teixeira, Marta O., additional, Teixeira, Marta A., additional, Antunes, Joana C., additional, and Amorim, M. Teresa P., additional
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- 2020
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9. Development of biodegradable electrospun dressing based on PVA/CA with antimicrobial potential for chronic wounds
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Teixeira, Marta A., primary, Tavares, Tânia D., additional, Ribeiro, Ana R. M., additional, Amorim, M. Teresa P., additional, and Felgueiras, Helena P., additional
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- 2020
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10. Chiral Analysis of Pesticides and Drugs of Environmental Concern: Biodegradation and Enantiomeric Fraction.
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Maia, Alexandra S., Ribeiro, Ana R., Castro, Paula M. L., and Tiritan, Maria Elizabeth
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SEPARATION of enantiomers , *PESTICIDE analysis , *CHIRALITY , *STEREOCHEMISTRY , *PHARMACEUTICAL chemistry - Abstract
The importance of stereochemistry for medicinal chemistry and pharmacology is well recognized and the dissimilar behavior of enantiomers is fully documented. Regarding the environment, the significance is equivalent since enantiomers of chiral organic pollutants can also differ in biodegradation processes and fate, as well as in ecotoxicity. This review comprises designed biodegradation studies of several chiral drugs and pesticides followed by enantioselective analytical methodologies to accurately measure the enantiomeric fraction (EF). The enantioselective monitoring of microcosms and laboratory-scale experiments with different environmental matrices is herein reported. Thus, this review focuses on the importance of evaluating the EF variation during biodegradation studies of chiral pharmaceuticals, drugs of abuse, and agrochemicals and has implications for the understanding of the environmental fate of chiral pollutants. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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11. A Pilot Study Combining Ultrafiltration with Ozonation for the Treatment of Secondary Urban Wastewater: Organic Micropollutants, Microbial Load and Biological Effects.
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Graça, Cátia A. L., Ribeirinho-Soares, Sara, Abreu-Silva, Joana, Ramos, Inês I., Ribeiro, Ana R., Castro-Silva, Sérgio M., Segundo, Marcela A., Manaia, Célia M., Nunes, Olga C., and Silva, Adrián M. T.
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MICROPOLLUTANTS ,IRRIGATION water quality ,SEWAGE ,OZONIZATION ,ULTRAFILTRATION ,WATER storage ,WATER currents ,WATER purification - Abstract
Ozonation followed by ultrafiltration (O
3 + UF) was employed at pilot scale for the treatment of secondary urban wastewater, envisaging its safe reuse for crop irrigation. Chemical contaminants of emerging concern (CECs) and priority substances (PSs), microbial load, estrogenic activity, cell viability and cellular metabolic activity were measured before and immediately after O3 + UF treatment. The microbial load was also evaluated after one-week storage of the treated water to assess potential bacteria regrowth. Among the organic micropollutants detected, only citalopram and isoproturon were not removed below the limit of quantification. The treatment was also effective in the reduction in the bacterial loads considering current legislation in water quality for irrigation (i.e., in terms of enterobacteria and nematode eggs). However, after seven days of storage, total heterotrophs regrew to levels close to the initial, with the concomitant increase in the genes 16S rRNA and intI1. The assessment of biological effects revealed similar water quality before and after treatment, meaning that O3 + UF did not produce detectable toxic by-products. Thus, the findings of this study indicate that the wastewater treated with this technology comply with the water quality standards for irrigation, even when stored up to one week, although improvements must be made to minimise microbial overgrowth. [ABSTRACT FROM AUTHOR]- Published
- 2020
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12. Red Propolis and Its Dyslipidemic Regulator Formononetin: Evaluation of Antioxidant Activity and Gastroprotective Effects in Rat Model of Gastric Ulcer.
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Mendonça, Marcio A. A. de, Ribeiro, Ana R. S., Lima, Adriana K. de, Bezerra, Gislaine B., Pinheiro, Malone S., Albuquerque-Júnior, Ricardo L. C. de, Gomes, Margarete Z., Padilha, Francine F., Thomazzi, Sara M., Novellino, Ettore, Santini, Antonello, Severino, Patricia, B. Souto, Eliana, and Cardoso, Juliana C.
- Abstract
Propolis has various pharmacological properties of clinical interest, and is also considered a functional food. In particular, hydroalcoholic extracts of red propolis (HERP), together with its isoflavonoid formononetin, have recognized antioxidant and anti-inflammatory properties, with known added value against dyslipidemia. In this study, we report the gastroprotective effects of HERP (50–500 mg/kg, p.o.) and formononetin (10 mg/kg, p.o.) in ethanol and non-steroidal anti-inflammatory drug-induced models of rat ulcer. The volume, pH, and total acidity were the evaluated gastric secretion parameters using the pylorus ligature model, together with the assessment of gastric mucus contents. The anti-Helicobacter pylori activities of HERP were evaluated using the agar-well diffusion method. In our experiments, HERP (250 and 500 mg/kg) and formononetin (10 mg/kg) reduced (p < 0.001) total lesion areas in the ethanol-induced rat ulcer model, and reduced (p < 0.05) ulcer indices in the indomethacin-induced rat ulcer model. Administration of HERP and formononetin to pylorus ligature models significantly decreased (p < 0.01) gastric secretion volumes and increased (p < 0.05) mucus production. We have also shown the antioxidant and anti-Helicobacter pylori activities of HERP. The obtained results indicate that HERP and formononetin are gastroprotective in acute ulcer models, suggesting a prominent role of formononetin in the effects of HERP. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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13. Physical, Thermal, and Antibacterial Effects of Active Essential Oils with Potential for Biomedical Applications Loaded onto Cellulose Acetate/Polycaprolactone Wet-Spun Microfibers.
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Felgueiras, Helena P., Homem, Natália C., Teixeira, Marta A., Ribeiro, Ana R. M., Antunes, Joana C., and Amorim, Maria Teresa P.
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MICROFIBERS ,CELLULOSE acetate ,ESSENTIAL oils ,POLYCAPROLACTONE ,STAPHYLOCOCCUS aureus infections ,THERMOGRAVIMETRY ,CELL membranes - Abstract
New approaches to deal with the growing concern associated with antibiotic-resistant bacteria are emerging daily. Essential oils (EOs) are natural antimicrobial substances with great potential to mitigate this situation. However, their volatile nature, in their liquid-free form, has restricted their generalized application in biomedicine. Here, we propose the use of cellulose acetate (CA)/polycaprolactone (PCL) wet-spun fibers as potential delivery platforms of selected EOs to fight infections caused by Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Twenty EOs were selected and screened for their minimal inhibitory concentration (MIC), using the antibiotic ampicillin as positive control. The cinnamon leaf oil (CLO), cajeput oil (CJO), and the clove oil (CO) were the most effective EOs, against the Gram-positive (MIC < 22.38 mg/mL) and the Gram-negative (MIC < 11.19 mg/mL) bacteria. Uniform microfibers were successfully wet-spun from CA/PCL with an averaged diameter of 53.9 ± 4.5 µm, and then modified by immersion with CLO, CJO and CO at 2 × MIC value. EOs incorporation was confirmed by UV-visible spectroscopy, Fourier-transformed infrared spectroscopy, and thermal gravimetric analysis. However, while microfibers contained ampicillin at MIC (control) after the 72 h modification, the CLO, CO and CJO-loaded fibers registered ≈ 14%, 66%, and 76% of their MIC value, respectively. Data showed that even at small amounts the EO-modified microfibers were effective against the tested bacteria, both by killing bacteria more quickly or by disrupting more easily their cytoplasmic membrane than ampicillin. Considering the amount immobilized, CLO-modified fibers were deemed the most effective from the EOs group. These results indicate that CA/PCL microfibers loaded with EOs can be easily produced with increased antibacterial action, envisioning their use as scaffolding materials for the treatment of infections. [ABSTRACT FROM AUTHOR]
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- 2020
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14. Spun Biotextiles in Tissue Engineering and Biomolecules Delivery Systems.
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Miranda, Catarina S., Ribeiro, Ana R. M., Homem, Natália C., and Felgueiras, Helena P.
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TISSUE engineering ,BIOENGINEERING ,BIOMOLECULES ,ARTIFICIAL implants ,BIOABSORBABLE implants - Abstract
Nowadays, tissue engineering is described as an interdisciplinary field that combines engineering principles and life sciences to generate implantable devices to repair, restore and/or improve functions of injured tissues. Such devices are designed to induce the interaction and integration of tissue and cells within the implantable matrices and are manufactured to meet the appropriate physical, mechanical and physiological local demands. Biodegradable constructs based on polymeric fibers are desirable for tissue engineering due to their large surface area, interconnectivity, open pore structure, and controlled mechanical strength. Additionally, biodegradable constructs are also very sought-out for biomolecule delivery systems with a target-directed action. In the present review, we explore the properties of some of the most common biodegradable polymers used in tissue engineering applications and biomolecule delivery systems and highlight their most important uses. [ABSTRACT FROM AUTHOR]
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- 2020
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15. Removal of Organic Micropollutants from a Municipal Wastewater Secondary Effluent by UVA-LED Photocatalytic Ozonation.
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Chávez, Ana M., Ribeiro, Ana R., Moreira, Nuno F. F., Silva, Adrián M. T., Rey, Ana, Álvarez, Pedro M., and Beltrán, Fernando J.
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MICROPOLLUTANTS , *OZONIZATION , *PACKED bed reactors , *SOLID phase extraction , *TANDEM mass spectrometry , *SEWAGE - Abstract
Numerous contaminants of emerging concern (CECs) have been found in different water bodies. Directive 2013/39/EU and Decision 2018/840/EU are consequently being implemented in the field of water policies. Twelve CECs (e.g., isoproturon, ciprofloxacin, and clarithromycin are among those listed) were detected in a municipal wastewater secondary effluent by means of solid phase extraction and ultra-high-performance liquid chromatography with tandem mass spectrometry (SPE-UHPLC-MS/MS). Different advanced oxidation processes (AOPs), based on the combination of ozone, UVA-LED and powdered TiO2, were investigated for their removal in a semi-batch operation. In addition, TiO2-coated glass rings (P25R) were characterized with different techniques (SEM, WDXRF) and used for continuous mode operation in a packed bed reactor (PBR). Among the AOPs studied, ozone-based processes were found to be more efficient than heterogeneous photocatalysis. A kinetic study was performed showing that direct ozonation is the main oxidation pathway for CEC removal. Ozone was successfully decomposed in combination with UVA-LED and P25R, resulting in an apparent rate constant of 3.2 × 10−2 s−1 higher than in the O3/LED system (1.0 × 10−3 s−1) or with ozone alone (8.6 × 10−5 s−1). Hydroxyl radical reaction could prevail over direct ozone reaction for the most refractory compounds (e.g., isoproturon). [ABSTRACT FROM AUTHOR]
- Published
- 2019
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16. Immobilised Cerium-Doped Zinc Oxide as a Photocatalyst for the Degradation of Antibiotics and the Inactivation of Antibiotic-Resistant Bacteria.
- Author
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Zammit, Ian, Vaiano, Vincenzo, Ribeiro, Ana R., Silva, Adrián M. T., Manaia, Célia M., and Rizzo, Luigi
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BACTERIAL inactivation ,ANTIBIOTICS ,ZINC oxide ,SEWAGE disposal plants ,ESCHERICHIA coli ,SALT - Abstract
The threat of antibiotic resistance to the wellbeing of societies is well established. Urban wastewater treatment plants (UWTPs) are recognised sources for antibiotic resistance dissemination in the environment. Herein a novel cerium-doped zinc oxide (Ce-ZnO) photocatalyst is compared to ZnO and the benchmark TiO
2 -P25 in the immobilised form on a metallic support, to evaluate a photocatalytic process as a possible tertiary treatment in UWTPs. The catalysts were compared for the removal of two antibiotics, trimethoprim (TMP) and sulfamethoxazole (SMX), and for the inactivation of Escherichia coli (E. coli) strain DH5-Alpha in isotonic sodium chloride solution and of autochthonous bacteria in real secondary wastewater. In real wastewater, E. coli and other coliforms were monitored, as well as the respective fractions resistant to ofloxacin and azithromycin. In parallel, Pseudomonas aeruginosa and the respective sub-population resistant to ofloxacin or ciprofloxacin were also monitored. Photocatalysis with both ZnO and Ce-ZnO was faster than using TiO2 -P25 at degrading the antibiotics, with Ce-ZnO the fastest against SMX but slower than undoped ZnO in the removal of TMP. Ce-ZnO catalyst reuse in the immobilised form produced somewhat slower kinetics maintained >50% of the initial activity, even after five cycles of use. Approximately 3 log10 inactivation of E. coli in isotonic sodium chloride water was recorded with reproducible results. In the removal of autochthonous bacteria in real wastewater, Ce-ZnO performed better (more than 2 log values higher) than TiO2 -P25. In all cases, E. coli and other coliforms, including their resistant subpopulations, were inactivated at a higher rate than P. aeruginosa. With short reaction times no evidence for enrichment of resistance was observed, yet with extended reaction times low levels of bacterial loads were not further inactivated. Overall, Ce-ZnO is an easy and cheap photocatalyst to produce and immobilise and the one that showed higher activity than the industry standard TiO2 -P25 against the tested antibiotics and bacteria, including antibiotic-resistant bacteria. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
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