1. Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach.
- Author
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Alves, Rita, Pimentel, Filipa, Nouws, Henri, Correr, Wagner, González-García, María, Oliveira, M., and Delerue-Matos, Cristina
- Subjects
PEANUT allergy ,BIOSENSORS ,VOLTAMMETRY ,ALLERGENS ,IMMUNOASSAY - Abstract
A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
- Published
- 2015
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