1. Effects of cryopreservation on antiviral responses of primary airway epithelial cells.
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Taka, Styliani, Nikopoulou, Chrysa, Polyzos, Alexandros, Megremis, Spyridon, Skevaki, Chrysanthi L., Roumpedaki, Eirini, Trochoutsou, Aikaterini, Thanos, Dimitris, and Papadopoulos, Nikolaos G.
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EPITHELIAL cells ,WHEEZE ,STAT proteins ,CRYOPRESERVATION of organs, tissues, etc. - Abstract
Keywords: airway epithelium; cryopreservation; human rhinovirus EN airway epithelium cryopreservation human rhinovirus 1486 1489 4 06/01/20 20200601 NES 200601 To the Editor, Human epithelial cell lines (eg, A549 and BEAS-2B cells), as well as primary nasal and bronchial epithelial cells, have been used extensively to explore the role of virus-host interactions in respiratory allergic diseases.[1] In vitro cell cultures of primary airway epithelial cells from patients are a key model for these studies. In RV-infected cells, 45 genes were differentially expressed (36 higher and 9 lower in fresh compared to cryopreserved cells) (Figure C, Table S2B). In the last decades, epithelial infection models have evolved from using cell lines[5] to submerged primary cells[6] to air-liquid interphase differentiated cells.[7] At the same time, utilization of cryopreserved cells or tissues has increased considerably. [Extracted from the article]
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- 2020
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