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Your search keyword '"de-los-Santos-Álvarez, Noemí"' showing total 36 results
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36 results on '"de-los-Santos-Álvarez, Noemí"'

14. First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin

Author

Muñoz-San Martín, Cristina, primary, Montero-Calle, Ana, additional, Garranzo-Asensio, María, additional, Gamella, Maria, additional, Pérez-Ginés, Víctor, additional, Pedrero, María, additional, Pingarrón, José M., additional, Barderas, Rodrigo, additional, de-los-Santos-Álvarez, Noemí, additional, Lobo-Castañón, María Jesús, additional, and Campuzano, Susana, additional

Published
2022
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32. On‐Gold Recombinase Polymerase Primer Elongation for Electrochemical Detection of Bacterial Genome: Mechanism Insights and Influencing Factors.

Author

Sánchez‐Salcedo, Raquel, Miranda‐Castro, Rebeca, de los Santos‐Álvarez, Noemí, and Lobo‐Castañón, María Jesús

Subjects
PATHOGENIC microorganisms, POLYMERASE chain reaction, OLIGONUCLEOTIDES, GENOMES, SALMONELLA
Abstract

The detection of bacterial pathogens may be accomplished through their genome material. With that aim, current methods usually rely on polymerase chain reaction (PCR) amplifications, which are time consuming and require specialist equipment. Here, we optimized an on‐chip isothermal recombinase polymerase amplification (RPA) with chronoamperometric detection, which could be run with simple equipment. A short thiol‐modified oligonucleotide, complementary to a fragment of bipA gene of Salmonella spp. is chemisorbed onto the gold chip surface together with p‐aminothiophenol as a blocking agent. Remarkably, we demonstrate that, in the presence of Salmonella genome, this oligonucleotide, acting as a primer in RPA, is directly elongated on the surface, copying the genome and giving rise to an on‐surface enzyme‐tagged dsDNA. After 30 min of isothermal amplification, the chronoamperometric signal, related to one of the products of the enzyme reaction, increased with the amount of Salmonella genome, which allowed the detection of 105 copies. The results of this study may be of general utility in the design of sensors for detecting other bacteria. Under the influence: The mechanism for the selective and isothermal amplification with electrochemical detection of Salmonella genome on gold chips is investigated. The recombinase‐polymerase reaction takes place directly on the surface, elongating the anchored primer, which enables the detection of 105 genome units. [ABSTRACT FROM AUTHOR]

Published
2019
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36. Nanostructures in Hydrogen Peroxide Sensing.

Author

Trujillo, Ricardo Matias, Barraza, Daniela Estefanía, Zamora, Martin Lucas, Cattani-Scholz, Anna, Madrid, Rossana Elena, and de-los-Santos-Álvarez, Noemí

Subjects
CHEMICAL processes, NANOSTRUCTURES, ELECTROCHEMICAL sensors, MECHANICAL properties of condensed matter, METAL nanoparticles, HYDROGEN peroxide
Abstract

In recent years, several devices have been developed for the direct measurement of hydrogen peroxide (H 2 O 2 ), a key compound in biological processes and an important chemical reagent in industrial applications. Classical enzymatic biosensors for H 2 O 2 have been recently outclassed by electrochemical sensors that take advantage of material properties in the nano range. Electrodes with metal nanoparticles (NPs) such as Pt, Au, Pd and Ag have been widely used, often in combination with organic and inorganic molecules to improve the sensing capabilities. In this review, we present an overview of nanomaterials, molecules, polymers, and transduction methods used in the optimization of electrochemical sensors for H 2 O 2 sensing. The different devices are compared on the basis of the sensitivity values, the limit of detection (LOD) and the linear range of application reported in the literature. The review aims to provide an overview of the advantages associated with different nanostructures to assess which one best suits a target application. [ABSTRACT FROM AUTHOR]

Published
2021
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