1. A mass spectrometric method for in-depth profiling of phosphoinositide regioisomers and their disease-associated regulation.
- Author
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Morioka, Shin, Nakanishi, Hiroki, Yamamoto, Toshiyoshi, Hasegawa, Junya, Tokuda, Emi, Hikita, Tomoya, Sakihara, Tomoko, Kugii, Yuuki, Oneyama, Chitose, Yamazaki, Masakazu, Suzuki, Akira, Sasaki, Junko, and Sasaki, Takehiko
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MEMBRANE lipids ,MASS spectrometry ,ISOMERS ,PROSTATE cancer ,EXOSOMES ,PHOSPHOINOSITIDES ,COLUMN chromatography - Abstract
Phosphoinositides are a family of membrane lipids essential for many biological and pathological processes. Due to the existence of multiple phosphoinositide regioisomers and their low intracellular concentrations, profiling these lipids and linking a specific acyl variant to a change in biological state have been difficult. To enable the comprehensive analysis of phosphoinositide phosphorylation status and acyl chain identity, we develop PRMC-MS (Phosphoinositide Regioisomer Measurement by Chiral column chromatography and Mass Spectrometry). Using this method, we reveal a severe skewing in acyl chains in phosphoinositides in Pten-deficient prostate cancer tissues, extracellular mobilization of phosphoinositides upon expression of oncogenic PIK3CA, and a unique profile for exosomal phosphoinositides. Thus, our approach allows characterizing the dynamics of phosphoinositide acyl variants in intracellular and extracellular milieus. Different phosphoinositide isomers are involved in a variety of physiological and pathological processes. Here, the authors combine chiral column chromatography and mass spectrometry to measure phosphoinositide regioisomers, revealing their dynamic changes in intra- and extracellular cancer cell milieus. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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