Search

Metastasising cells express the intermediate filament protein vimentin, which is used to diagnose invasive tumours in the clinic. We aimed to clarify how vimentin regulates the motility of metastasising fibroblasts. STED super-resolution microscopy, live-cell imaging and quantitative proteomics revealed that oncogene-expressing and metastasising fibroblasts show a less-elongated cell shape, reduced cell spreading, increased cell migration speed, reduced directionality, and stronger coupling between these migration parameters compared to normal control cells. In total, we identified and compared 555 proteins in the vimentin interactome. In metastasising cells, the levels of keratin 18 and Rab5C were increased, while those of actin and collagen were decreased. Inhibition of HDAC6 reversed the shape, spreading and migration phenotypes of metastasising cells back to normal. Inhibition of HDAC6 also decreased the levels of talin 1, tropomyosin, Rab GDI beta, collagen and emilin 1 in the vimentin interactome, and partially reversed the nanoscale vimentin organisation in oncogene-expressing cells. These findings describe the changes in the vimentin interactome and nanoscale distribution that accompany the defective cell shape, spreading and migration of metastasising cells. These results support the hypothesis that oncogenes can act through HDAC6 to regulate the vimentin binding of the cytoskeletal and cell-extracellular matrix adhesion components that contribute to the defective motility of metastasising cells., QC 20220413

Zebrafish, a popular organism for studying embryonic development and for modeling human diseases, has so far lacked a systematic functional annotation program akin to those in other animal models. To address this, we formed the international DANIO-CODE consortium and created a central repository to store and process zebrafish developmental functional genomic data. Our data coordination center (https://danio-code.zfin.org) combines a total of 1,802 sets of unpublished and re-analyzed published genomic data, which we used to improve existing annotations and show its utility in experimental design. We identified over 140,000 cis-regulatory elements throughout development, including classes with distinct features dependent on their activity in time and space. We delineated the distinct distance topology and chromatin features between regulatory elements active during zygotic genome activation and those active during organogenesis. Finally, we matched regulatory elements and epigenomic landscapes between zebrafish and mouse and predicted functional relationships between them beyond sequence similarity, thus extending the utility of zebrafish developmental genomics to mammals.

Zebrafish, a popular organism for studying embryonic development and for modeling human diseases, has so far lacked a systematic functional annotation program akin to those in other animal models. To address this, we formed the international DANIO-CODE consortium and created a central repository to store and process zebrafish developmental functional genomic data. Our data coordination center (https://danio-code.zfin.org) combines a total of 1,802 sets of unpublished and re-analyzed published genomic data, which we used to improve existing annotations and show its utility in experimental design. We identified over 140,000 cis-regulatory elements throughout development, including classes with distinct features dependent on their activity in time and space. We delineated the distinct distance topology and chromatin features between regulatory elements active during zygotic genome activation and those active during organogenesis. Finally, we matched regulatory elements and epigenomic landscapes between zebrafish and mouse and predicted functional relationships between them beyond sequence similarity, thus extending the utility of zebrafish developmental genomics to mammals

About this book: In a fast-moving global industry how does anyone know what the next trend will be? To answer this question, fashion professionals engage in a systematic, analytical process to predict and understand changes in society and consumer behavior. Forecasting companies and developers collect information related to culture, the economy, politics, and technology that may influence future trends. Gleaning information wherever they can, online or from books, arts, music, movies, fashion and architecture, fashion forecasters research cultural indicators that signpost new ways of living, shopping, and designing. Fashion Trends offers a clear pathway into the theory and practice of forecasting fashion, using professional case studies to demonstrate each technique and concept. This revised edition includes expanded coverage of social media, crowd sourcing, digital influencers and the use of technology such as augmented reality, radio-frequency identification (RFID) and big data. With the rise of individualism, the authors also walk you through the 'end of fashion' and what comes next, including clothing subscription and rental services, the circular economy, transparency and traceability and the role of forecasting in provoking the desire for a sustainable lifestyle.

Models of communicative competence in a second language invoked in defining the construct of widely used tests of communicative language ability have drawn largely on the work of language specialists. The risk of exclusive reliance on language expertise to conceptualize, design and administer language tests is that test scores may carry meanings that are misaligned with the values of non-language specialists, that is, those without language expertise but perhaps with expert knowledge in the domain of concern. Neglect of the perspective of lay (i.e., non-linguistic) judges on language and communication is a serious validity concern, since they are the ultimate arbiters of what matters for effective communication in the relevant context of language use. The paper reports on three research studies exploring the validity of rating scales used to assess speaking performance on a number of high-stakes English-language tests developed for professional or general proficiency assessment purposes in Korea, Australia, China, and the UK. Drawing on Jacoby and McNamara's (1999) notion of “indigenous assessment”, each project attempted to identify the values underlying non-language specialists' judgements of spoken communication as they rated test performance or participated in focus-group workshops where they viewed and commented on video- or audio-recorded samples of performance in the relevant real-world domain. The findings of these studies raise the question of whether language can or should be assessed as object independently of the content which it conveys or without regard for the goal and context of the communication. The studies' findings also cast doubt on the notion that the native speaker should always serve as benchmark for judging communicative effectiveness, especially with tests of language for specific purposes, where native speakers and second-language learners alike may lack the requisite skills for the kind of effective interaction demanded by the context.

Models of communicative competence in a second language invoked in defining the construct of widely used tests of communicative language ability have drawn largely on the work of language specialists. The risk of exclusive reliance on language expertise to conceptualize, design and administer language tests is that test scores may carry meanings that are misaligned with the values of non-language specialists, that is, those without language expertise but perhaps with expert knowledge in the domain of concern. Neglect of the perspective of lay (i.e., non-linguistic) judges on language and communication is a serious validity concern, since they are the ultimate arbiters of what matters for effective communication in the relevant context of language use. The paper reports on three research studies exploring the validity of rating scales used to assess speaking performance on a number of high-stakes English-language tests developed for professional or general proficiency assessment purposes in Korea, Australia, China, and the UK. Drawing on Jacoby and McNamara's (1999) notion of “indigenous assessment”, each project attempted to identify the values underlying non-language specialists' judgements of spoken communication as they rated test performance or participated in focus-group workshops where they viewed and commented on video- or audio-recorded samples of performance in the relevant real-world domain. The findings of these studies raise the question of whether language can or should be assessed as object independently of the content which it conveys or without regard for the goal and context of the communication. The studies' findings also cast doubt on the notion that the native speaker should always serve as benchmark for judging communicative effectiveness, especially with tests of language for specific purposes, where native speakers and second-language learners alike may lack the requisite skills for the kind of effective interaction demanded by the context.