1. 1α,25-Dihydroxyvitamin D3 up-regulates IL-34 expression in SH-SY5Y neural cells.
- Author
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Dong Zhang, Miaomiao Li, Yang Dong, Xinhui Zhang, Xingyun Liu, Zhangming Chen, Yongji Zhu, Huiming Wang, Xuwen Liu, Jialiang Zhu, Yujun Shen, Korner, Heinrich, Songcheng Ying, Shengyun Fang, and Yuxian Shen
- Subjects
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GENE expression , *IMMUNE response , *ALZHEIMER'S disease , *CELL lines , *SENILE dementia - Abstract
Vitamin D supplementation is regarded as a novel approach to treat Alzheimer's disease, but the underlying mechanism remains elusive. The cytokine IL-34 provides strong neuroprotective and survival signals in brain injury and neurode-generation and could be an immunological mediator for the vitamin D-induced protection. The aim of this study was to investigate whether human IL-34 is up-regulated in neuronal cells by the hormonally active form of vitamin D, 1α,25-dihydroxyvitamin D3 [1α,25(OH)[sub 2]D[sub 3]]. We found that IL-34 was detectable in a variety of cell lines and its expression was strongly induced in SH-SY5Y neural cells in a dose- and time-dependent manner by 1α,25(OH)[sub 2]D[sub 3] through the vitamin D receptor (VDR). Furthermore, we identified the core promoter of IL-34 gene and a VDR binding site (CGCCCT) that was required for 1α,25(OH)[sub 2]D[sub 3]-induced IL-34 expression. These findings suggest that the induction of IL-34 expression by 1α,25(OH)[sub 2]D[sub 3] may constitute a mechanism that explains the protective function of vitamin D in Alzheimer's disease. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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