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The genus Bolboschoenus has been intensively studied from the morphological, ecological and physiological aspects, however, anatomical features of stems, leaves and epidermal structures of leaves have not been investigated sufficiently yet. The main objective of this study was to compare the four Bolboschoenus species growing in Serbia at the anatomical level and to define the most important anatomical characteristics for distinguishing these species. Descriptive, nonmetric multidimensional scaling and canonical discriminant analysis of the anatomical characters of leaves, stems and epidermal structures have been conducted for these purposes. The number and position of sclerenchyma strands in the leaves, the presence of bulliform cells in the epidermis, the number of translucent cell layers under the adaxial epidermis in the midrib area of the lamina, the number of aerial cavities in leaves and stems were the most significant anatomical characters for differentiation of species. The most differentiated species was B. glaucus, while B. laticarpus occupied an intermediate position between B. maritimus and B. planiculmis, on the one hand, and B. glaucus on the other. The combination of the anatomical features of leaves and stems and the epidermal structures had a more excellent taxonomic value than these three individual groups of features individually. [ABSTRACT FROM AUTHOR]

Encyclia is the second-largest genus in the neotropical subtribe Laeliinae (Orchidaceae) and has more than 150 species, which are characterized by fairly consistent flower morphology. Its taxonomy and species boundaries, however, seem to be still under debate. In the present study, we first examined the lip micromorphology of 61 species of Encyclia sensu stricto. We correlated our results with external flower morphology and phylogenetic analyses performed on a combined dataset that included both nuclear (ITS, Xdh, PhyC) and plastid markers (ycf1, rpl32, and trnL-trnF). Phylogenetic reconstruction showed that Encyclia sensu stricto species form a coherent, monophyletic group. However, it is difficult to determine the relationships between the different groups within one larger clade. The groups all form distinct lineages that evolved from a common ancestor. The UPGMA cluster analysis for the seven qualitative micromorphological features clearly divides the genus into two main groups, the larger of which is further subdivided into two subgroups. None of these, however, overlap with any of the phylogeographic units distinguished in previously published papers or in presented article. It is worth noting that the groups resulting from the UPGMA analysis cannot be defined by macromorphological features. The pattern of similarities between species, taking into account both macro- and micromorphological features, is eminently mosaic in nature, and only a multifaceted approach can explain this enigmatic group. [ABSTRACT FROM AUTHOR]

The present study aimed to conduct anatomical and micromorphological analysis of inflorescences and flowers of six Verbena species native to South America (Verbenaceae): V. gracilescens from series Verbena, and V. bonariensis, V. intermedia, V. litoralis, V. montevidensis and V. rigida, all five from series Pachystachyae. The results showed that the rachis transverse section exhibits unistratified epidermis and hypodermis, eustele and parenchymatic pith. The chlorenchymatous cortex can be continuous or discontinuous. The floral bract shows unistratified epidermis, homogeneous mesophyll and parenchymatous sheath in the central vascular bundle. The perianth exhibits scarce homogeneous mesophyll. In relation to trichomes, non-glandular and glandular are occur in the inflorescence structures of all the studied species. Verbena bonariensis, V. rigida and V. intermedia have the same non-glandular (types I and II) and glandular (types IV-VII) trichomes. Non-glandular appressed trichomes (type III) were observed only in V. gracilescens, V. montevidensis and V. litoralis. Verbena gracilescens (Verbena series) and V. montevidensis (Pachystachyae series) show lax inflorescence with spaced flowers and only short stalked glandular and appressed non-glandular trichomes, with scarce density. The other studied species of series Pachystachyae exhibit dense inflorescence and all glandular and non-glandular trichomes types. The six studied species can be characterized by different trichome types in the inflorescences and anatomy of the rachis. The results have taxonomical importance, furthermore since several species of Verbena have medicinal properties these studies are a potential tool for the correct identification of the raw drug, preventing adulteration. [ABSTRACT FROM AUTHOR]

In this study, endemic Onosma mollis DC. it is aimed to examine the anatomical and ecological features of (Boraginaceae) plant in detail and to be compared with the other studies. According to the cross-sections, the root is secondary. Primary pith rays that 2-3 layered are observed. Epidermis is single-layered in stem transverse section. Eglandular hairs in the epidermis are simple, with one and two cells. Glandular hairs are capitate, digitate and with two cells. Just under the epidermis, 2-3 rows of collenchyma cells are formed. Both the upper and the lower surface of leaf is covered with numerous eglandular hairs. Eglandular hairs are 2-3 branched. Glandular hairs are of capitate, digitate and peltate type. The habitat of the O. mollis plant is mountain slopes, meadows, and prairies. The soil in which the species grows is clay and loamy. It is also classified as slightly alkaline, salty, calcareous, low phosphorus and high in potassium. According to its organic matter content, it is in the group of good humus soils. [ABSTRACT FROM AUTHOR]

Anatomical characteristics regenerant plantlet of Arabica coffee (Coffea arabica L.) were observed to determine the difference of plantlet performance between Sigararutang and Maragogige grown in shooting and rooting medium. Transverse sections of the fresh roots, stems and leaves of three-month-old plantlets from somatic embryos were collected and used for the study. Sigararutang and Maragogipe as the plantlet materials were chosen based on the bean size and the origin. Stomata were microscopically observed on the abaxial leaf paradermal section. A conformity test to compare between plantlet and the parent plant was observed to perform genetic stability. Assessment of genetic stability was measured by using the sequence of trnL (UAA) region. The result showed that all the anatomical roots, stems and leaves of the Maragogipe plantlet have a greater number than Sigararutang (root diameter, cortex thickness, distance of long stele, distance of short stele, endodermis thickness, stem diameter, cortex thickness, maximum stele diameter, minimum stele diameter, epidermis thickness, diameter of stomatal closing, length of stomatal closing, total stomatal density, adaxial epidermis density, midrib thickness, adaxial epidermis thickness, abaxial epidermis thickness, diameter of the vascular bundles, lamina thickness), except of epidermis thickness, diameter of the vascular bundles, diameter of stomatal aperture, diameter of stomatal opening, length of stomatal opening and abaxial epidermis density. Taxonomists may be able to use these anatomic traits as supplementary proof in the determination of Arabica coffee. Molecular analysis showed that there were genetically identical organisms between the plantlet and the parent plant. It was indicated there was no somaclonal variation during somatic embryogenesis in the micropropagation of Arabica coffee. [ABSTRACT FROM AUTHOR]

Premise of research. Chusquea Kunth is the most diverse bamboo genus worldwide and comprises 198 species classified into five subgenera. Chusquea subg. Magnifoliae and Chusquea subg. Platonia together include 26 taxa that have been studied on the basis of incomplete or fragmentary material, and complete keys to their taxonomic identification based on vegetative and reproductive characters are still pending. Since the identification of woody bamboos often relies mainly on vegetative characters, the addition of foliage leaf blade anatomy and micromorphology characters provides valuable information to be considered. Methodology. Anatomical and micromorphological studies were performed on the basis of herbarium material. Foliage leaf blade abaxial epidermal and transverse section characters are described and compared for 11 taxa of Chusquea subg. Magnifoliae and 14 of Chusquea subg. Platonia to enhance the currently available descriptions. Pivotal results. Regarding foliage leaf blade anatomy in transverse section, no single character turned out to be exclusive to a species or subgenus. All taxa studied here are recognizable from a combination of anatomical characters. Of note is that intercostal sclerenchyma is recorded for the first time in the subtribe Chusqueinae. When micromorphological characters of the foliage leaf blade abaxial epidermis are considered, a few potential autapomorphies may be recognized for a few species. Conclusions. On the basis of anatomical and micromorphological studies, characters of taxonomic value are presented in comparative tables. A key to the identification of the species of Chusquea subg. Magnifoliae and Chusquea subg. Platonia was elaborated and is presented here for the first time as an additional tool to corroborate the taxonomic identity of sterile or poorly collected specimens. [ABSTRACT FROM AUTHOR]

Annotation: The morphological and anatomical structure of the leaves of some plant species of the Genus Asanthophyllum C.A. Mey living in different habitats was studied and analyzed. We have studied the morphology of leaf hairs of 25 plant species, many of which were taken from 2-4 geographical points of their range, in order to determine the influence of environmental conditions on the type, morphology, and degree of drooping. As a result of the research, the following conclusions were made, in species A korshinskyi, A. borsezowii, A. elatius, A. cyrtostegium common in deserts and sec. Turbinaria - bare leaves are formed in Kopetdag, that the leaves of A. albidum, A. subglabrum, A. tenuifolium, A. stenostegium, A. adenophorum, A. krascheninikovii, A. brewibracteatum, A. aculeatum, A. borsezowii, A. serawchanicum, A.coloratum, A. jarmolenkii are pubescent only with simple hairs, and in A. pungens, A.lilacinum, A.pulchrum, A.korolkovii the leaves are pubescent with both simple and glandular hairs and trichomes. The shown differences of plants are associated with the ecological conditions of the place of growth. [ABSTRACT FROM AUTHOR]

Premise of research. Understanding the relationships of early euphyllophyte lineages of Devonian age is hampered by a lack of comparable characters among taxa, most providing either morphology or anatomy or, if both, being incompletely preserved. Wilhowia phocarum Gensel gen. et sp. nov., of Early Devonian, basal Emsian age and from the north shore of Gaspé Bay, Quebec, Canada, is a comparatively large plant that is reconstructed from partially permineralized adpressions of plant remains representing a unique combination of morphological and anatomical features. Methodology. Macrofossils were prepared by dégagement, and some compressed remains were removed by maceration in HCl and HF and studied by LM and SEM. Pyritized or limonitized portions of axes were removed from specimens and embedded, and serial thin sections were prepared following established techniques. Some were etched in HNO3 and examined with SEM. Pivotal results. Specimens consist of up to 30-cm-long and 1-cm-wide axes that produce distantly spaced, helically arranged, long first-order axes on which helically borne dichotomous ultimate axes occur. The ultimate axes divide cruciately several times, terminating in acute tips or clusters of fusiform sporangia. Sporangia are paired, dehisce longitudinally, and produce trilete spores with fine ornament outside contact areas. Permineralized main axes show (usually) an epidermis, a mostly uniform outer cortex, and a three-ribbed actinostele in which all tracheids show either P-type or occasional circular pits. Lateral traces depart from the tips of the ribs singly or in pairs and are oval in shape. Single and paired first-order axes result from different patterns of trace formation. Vascular traces to much-divided ultimate axes are circular to oval. Secondary tissues are absent. Conclusions. Wilhowia phocarum is distinguished from all previously described Early Devonian euphyllophyte taxa in its combination of anatomy and branching architecture, thus not fitting any currently recognized euphyllophyte lineage. Despite its lengthy centimeter-wide axes, it shows no evidence of secondary tissues, suggesting that its actinostelic vasculature may have provided most of its support. In comparison with several coeval taxa, the external morphology and distinctive anatomy of W. phocarum demonstrate the importance of having characters of both preservation types, and it appears to be a unique lineage within the euphyllophytes. These types of studies provide a better understanding of the complex mosaic of features manifested among early euphyllophytes. Anatomical changes from the main axis to first-order lateral axes suggest differentiation in branching systems leading to the evolution of leaves. [ABSTRACT FROM AUTHOR]

In this paper leaf structure of 4 Taxus species and varieties (Taxus baccata, T. baccata var. fastigiata, T. brevifolia, Taxus cuspidata) was investigated. Histological and micromorphological studies were carried out by light and scanning electron microscopy. From the histo-anatomical point of view, the leaf shows a general structural pattern with palisade and spongy parenchyma. This is common to all investigated taxa with quantitative variations. The stomata are located only in the lower epidermis (hypostomatic leaves), being disposed on 2 bands, with different widths, on one side and the other of the midrib. The papillae of the cells from the lower epidermis are also visible next to the median rib, with the exception of the species T. brevifolia. The palisade parenchyma is bilayered, with short cells. The structural characters of the leaves have taxonomical significance. The importance of the structural features of the leaf in the adaptation of the species to the living environment were emphasized. [ABSTRACT FROM AUTHOR]

Plant's stomatal physiology and anatomical features are highly plastic and are influenced by diverse environmental signals including the concentration of atmospheric CO2 and nutrient availability. Recent reports suggest that the form of nitrogen (N) is a determinant of plant growth and nutrient nitrogen use efficiency (NUE) under elevated CO2 (EC). Previously, we found that high nitrate availability resulted in early senescence, enhanced reactive oxygen species (ROS), and reactive nitrogen species (RNS) production and also that mixed nutrition of nitrate and ammonium ions were beneficial than sole nitrate nutrition in wheat. In this study, the interactive effects of different N forms (nitrate, ammonium, mixed nutrition of nitrate, and ammonium) and EC on epidermal and stomatal morphology were analyzed. Wheat seedlings were grown at two different CO2 levels and supplied with media devoid of N (N0) or with nitrate–N (NN), mixed nutrition of ammonium and nitrate (MN), or only ammonium-N (AN). The stoma length increased significantly in nitrate nutrition with a consistent reduction in stoma width. Guard cell length was higher in EC treatment as compared to AC. The guard cell width was maximum in AN-grown plants at EC. Epidermal cell density and stomatal density were lower at EC. Nitrate nutrition increased the stomatal area at EC while the reverse was true for MN and AN. Wheat plants fertilized with AN showed a higher accumulation of superoxide radical (SOR) at EC, while in NN treatment, the accumulation of hydrogen peroxide (H2O2) was higher at EC. Reactive oxygen species, particularly H2O2, can trigger mitogen-activated protein kinase (MAPK) mediated signaling and its crosstalk with abscisic acid (ABA) signaling to regulate stomatal anatomy in nitrate-fed plants. The SOR accumulation in ammonium- and ammonium nitrate–fed plants and H2O2 in NN-fed plants might finely regulate the sensitivity of stomata to alter water/nutrient use efficiency and productivity under EC. The data reveals that the variation in anatomical attributes viz. cell length, number of cells, etc. affected the leaf growth responses to EC and forms of N nutrition. These attributes are fine targets for effective manipulation of growth responses to EC. [ABSTRACT FROM AUTHOR]

Premise: Gall‐inducing organisms change the development of their host plant organs, resulting in ontogenetic patterns not observed in the non‐galled plants. Distinct taxa induce galls on Schinus spp., manipulating meristematic patterns in the host plant in distinct ways. Here we report ontogenetic novelties induced in the lateral buds of S. engleri by Eucecidoses minutanus, a Cecidosidae, whose galls have been poorly understood. Methods: The anatomy, histochemistry, and histometry of galls in distinct phases of development, non‐galled buds, and stems of Schinus engleri were analyzed in parallel with the instars of E. minutanus to detail the morphogenetic changes in the host with each larval stage. Results: Ontogenetic phases of the galls were intricately associated with larval development. First and second‐instar larvae induced pericycle and pith cells to dedifferentiate into the gall inner meristem, where hyperplasia and cell hypertrophy characterized the growth and development phase of the gall. The innermost layers were lipid‐rich nutritive cells that lined the larval chamber. Additional vascular bundle rows were produced in young galls. Third and fourth instar‐larvae were associated with the gall maturation phase: centripetal lignification of the outer parenchyma cell layers, epidermal stratification, and activation of a cambium‐like meristem (CLM). The CLM activity resulted in new layers of nutritive cells that differentiated inward as the first layers of nutritive cells were consumed by E. minutanus larvae, and, also, in more parenchyma cell layers that formed outward. All tissues between the innermost layer of nutritive tissue that surround the gall chamber and the outermost layer of the dermal system that externally covers the gall form the gall wall, and increased in thickness until the end of gall maturation. Conclusions: E. minutanus induces a structurally complex globoid stem gall, modifying all host plant tissues and stimulating a novel meristematic pattern in S. engleri. The gall developmental stages are each related to specific gall‐inducing instars, as gall development progresses according to the development of E. minutanus. [ABSTRACT FROM AUTHOR]

Background and aims: In Argentina, the genus Bambusa comprises nine introduced taxa, of which the most cultivated are B. multiplex, B. tuldoides and B. vulgaris cv. vittata. Bambusa plants are characterized by non-seasonal blooms, after long and variable vegetative periods. The study of the foliage leaf anatomy of woody bamboos is thus usefulness in the taxonomy of Bambusoideae. In order to characterize each species, the micromorphology, anatomy, and ultrastructure of foliage leaf blades are studied. M&M: With the aid of optical, scanning electron and transmission electron microscopy, the anatomy of foliage leaf blades of the three species are analysed. Qualitative and quantitative parameters of transverse and longitudinal sections and dissociated material are documented. Results: The dimensions of the leaf blade showed significant differences between the three species of Bambusa studied. The micromorphology of the foliage leaf blade surface exhibits differential characters between the abaxial and adaxial epidermis between species, such as the presence or absence of microhairs, macrohairs and prickles; silica cell type; degree of cohesion and number of papillae surrounding the stomatal apparatus. The dimensions and characteristics of the fusoid cells and fibers show differences between the species, as well as the micrometric parameters as seen in cross sections. Conclusions: The morphoanatomical features allows the characterization of the Bambusa species studied. [ABSTRACT FROM AUTHOR]

In this study, detailed morphological and anatomical features of Isatis quadrialata were examined. With anatomical analysis, transverse sections of root, stem and leaf were examined. Epidermis is a protective tissue in root transverse sections. Transverse sections of stem contain one layered epidermis, cortex parenchyma, vascular bundles and pith cells. The cross sections of leaves have two layered palisade parenchyma and spongy parenchyma with bifacial mesophyll. Transverse sections of fruit have one layered exocarp, multi layered mesocarp and endocarp. In addition, fruit and seed micromorphologies were studied. The fruit surface ornamentation is reticulate while the seed surface ornamentation is blister. The anatomical characteristics of Isatis quadrialata were determined of taxonomic importance. [ABSTRACT FROM AUTHOR]

The equine hoof capsule, composed of modified epidermis and dermis, is vital for protecting the third phalanx from forces of locomotion. There are descriptions of laminitis, defined as inflammation of sensitive hoof tissues but recognized as pathologic changes with or without inflammatory mediators, in the earliest records of domesticated horses. Laminitis can range from mild to serious, and signs can be acute, chronic, or transition from acute, severe inflammation to permanently abnormal tissue. Damage within the intricate dermal and epidermal connections of the primary and secondary lamellae is often associated with lifelong changes in hoof growth, repair, and conformation. Decades of research contribute to contemporary standards of care that include systemic and local therapies as well as mechanical hoof support. Despite this, consistent mechanisms to restore healthy tissue formation following a laminitic insult are lacking. Endogenous and exogenous progenitor cell contributions to healthy tissue formation is established for most tissues. There is comparably little information about equine hoof progenitor cells. Equine hoof anatomy, laminitis, and progenitor cells are covered in this review. The potential of progenitor cells to advance in vitro equine hoof tissue models and translate to clinical therapies may significantly improve prevention and treatment of a devastating condition that has afflicted equine companions throughout history. [ABSTRACT FROM AUTHOR]

The goal of this inquiry activity is to evaluate a variety of skin-care products on simulated skin cells using Orbeez, a brand of superabsorbent polymers. These small beads can quickly absorb water but will dry out in the air, thus perfectly representing our own skin cells. This property makes Orbeez an acceptable model for human skin, and test material for evaluating various skin-care products. Students will observe the quantitative changes that happened to the mass of the samples to determine the percent change. They will also observe the qualitative changes that happened throughout the lab investigation and record their observations in a lab notebook. After completing the experiment, students will create a presentation of the quantitative and qualitative results. [ABSTRACT FROM AUTHOR]

Premise of research. Periderm is a protective secondary tissue that replaces the epidermis in stems and roots having secondary growth. The first periderm most commonly originates immediately below the epidermis, but in some species, it arises deeper in the stem, usually in the primary phloem. Periderm is a common feature of extant plants and has been reported in numerous fossil taxa. Here we investigate the presence of this tissue in a Carboniferous representative of the cladoxylopsids, an extinct group related to the ferns s.l. Methodology. We describe anatomically preserved stems of Cladoxylon taeniatum with a well-preserved periderm from Tournaisian deposits in France and Germany. We also provide a short review of the current state of our knowledge on periderm production in Paleozoic plants, especially in relation to secondary vascular growth. Pivotal results. The new specimens are 7–15 mm in diameter. They have a well-developed secondary xylem and a thin periderm that has a deep-seated origin in the innermost part of the cortex. This shows that Cladoxylon had the ability to accommodate secondary vascular growth with the production of a periderm that replaced the cortex and epidermis. Cladoxylon taeniatum is, to date, the only cladoxylopsid in which this feature is described, and it increases the diversity of anatomical strategies for secondary growth documented in the group. Changes in the nature and geometrical distribution of tissue between typical stems with a cortex and stems with a periderm suggest that C. taeniatum was non-self-supporting, a growth habit that also differs from that of most other older cladoxylopsids. Conclusions. The new specimens increase the diversity of Paleozoic plants that produced both secondary vascular tissues and a periderm. Our review highlights the fact that anatomical homologies between the structures called "periderm" in these various taxa remain unclear; numerous questions about the origins of the periderm and the possible relation between secondary vascular growth and periderm production remain. [ABSTRACT FROM AUTHOR]

Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) is endemic to Turkey and described by laciniae linear leaves, pale lilac flowers, sessile follicles and called as "boz mahmuz". Plant material was collected from Ayaş (Ankara-Turkey). The transverse sections of leaf, stem and root were examined anatomically in this study. The leaf is monofacial and has a 1-layer epidermis. Ranunculaceous stomata and unicellular non-glandular hairs were observed in the epidermis. The stem is characterized by a 1-layer of epidermis, glandular and non-glandular hairs. Cortex parenchyma contains dense starch. Pericyclic sclerenchymatous ring and concave xylem are observed. The root has periderm, pericyclic sclerenchymatous ring and vessels embedded in sclerenchymatous pith cells. [ABSTRACT FROM AUTHOR]

Camoensia scandens is a papilionoid legume inserted in the core genistoid clade. It has large, crepuscular, scented flowers but the corolla is non-papilionaceous, which deviates from the pattern found in the subfamily. The vexillum has a folded claw, forming a tube, which is opposed to the androecium opening; all petals have yellow-gold crinkled margins. In addition, there is a long hypanthium, which stores a translucid liquid. The goal of this study is to elucidate the ontogenetic pathways that result in such a peculiar flower and the glands responsible for the sweet fragrance of the petals. Floral buds and flowers were processed for SEM, TEM and light microscopy analyses. Five sepals arise unidirectionally followed by five petals that initiate simultaneously. After the petals, 11 stamens emerge unidirectionally; a pair of adaxial stamens is opposite to the vexillum. In the intermediate developmental stages the sepals unite basally; the two adaxial sepals unite with each other to a greater extent than with the other sepals. The filaments are basally connate, forming a tube with an adaxial opening at the base. The carpel emerges concomitantly with the two abaxial antepetalous stamens. The long hypanthium forms from the outer floral organs (base of the sepals, petals, filaments) and is attached to the base of the stipe. The corolla is noticeable in the intermediate stages of development. The crinkled golden margins house scent glands formed of a secretory epidermis with secretory trichomes and secretory subepidermal cells. The odor is composed of neutral polysaccharides, nitrogenous substances and essential oils. An extensive nectariferous region is found on the inner surface of the hypanthial tube. The nectar is translucent, viscous and released through large pores. The comparison of our data with that of other genistoid flowers enabled discussions about the pollination and systematics of the group. [ABSTRACT FROM AUTHOR]

This is the first contribution about the histochemistry of vegetative and reproductive aerial organs in the genus Piper L. Piper malgassicum accumulates alkaloids and terpenes in the epidermis and the underlying layers of parenchyma, both in the leaves, in the stems and in anthers. Some idioblasts appear to contain a large amount of secondary metabolites. The micro-anatomical analysis showed peculiar secretory structures both in the leaves, in the anthers and in the ovary. Several lipid aggregates, alkaloid droplets and calcium oxalate crystals were observed in leaves and stems, indicating their role in defence strategies, mechanical support, and pollinators attraction. In the anthers, we observed elaioplasts whose content suggest an alternative and indirect function in pollination and defence against micro-organisms. Besides, some lipid aggregates surrounded by microtubules, detected in the anthers, were recognized as lipotubuloids. The tapetum was of secretory type. Alkaloids and terpenes were widely distributed in the plant confirming the important biological role of this type of biomolecules and its functional range. In the anthers, terpene and polyphenol inclusions appeared particularly abundant in the epidermal layer, whereas calcium oxalate crystals were observed close to the ovule in the ovary at maturity. [ABSTRACT FROM AUTHOR]

The epidermal characters of leaves and anatomical characters of petioles and young stems of some members of the genus Chrysophyllum, viz. C. albidum, C. perpulchrum, C. cainito, and C. delevoyi were analyzed to determine their diagnostic features. Paracytic stomata, crystal sand, prismatic crystals, secretory canals occurred in all the species. All the species are hypostomatic except C. perpulchrum. The midribs of all species have an open semi-circular vascular system except in C. cainito with a closed system. Midrib and petiole of C. albidum and C. cainito have non-glandular T-shaped trichomes. Accessory bundles are only seen in the petioles of C. delevoyi, and in the midribs of C. albidum and C. cainito. The midrib and petiole of C. delevoyi and C. albidum have a central bundle. Laticifers, prismatic and sand crystals occur mainly in the cortical cells, pith cells, xylem, phloem, and mesophyll. Lamina of all species studied showed uniseriate epidermis except C. cainito which has two layers of the adaxial epidermis. The stomatal index, the ratio of the spongy to palisade mesophyll thickness, the ratio of cortex thickness, outline, number, and arrangement of the vascular bundles in the midrib, petiole, and young stem differ and are valuable diagnostic features in Chrysophyllum. [ABSTRACT FROM AUTHOR]

Qualitative and quantitative studies were done on leaf epidermal characteristics with special reference to stomatal features to find out the impact of environmental condition on twenty four accessions of Cynodon dactylon (L.) Pers. collected from different ecological habitats of Bangladesh. The foliar epidermal peels from both surfaces of mature leaves were observed under microscope. The leaves were found to be amphistomatic and stomata were paracytic type. The epidermal cells in this study were found to be sinuous. Silica bodies were found to be saddle and cross shaped. Prickles angular were pointed at the tip. Macro-hairs were present in all the accessions, but no micro-hair was found both adaxially or abaxially. Stomatal frequency and stomatal index were found to vary from accession to accession on both adaxial and abaxial surface of leaves, and the differences were statistically significant in most of the cases. [ABSTRACT FROM AUTHOR]

The use and search for new methods and ways to reduce negative herbicidal effect on crops is a key factor in increasing the level of yield and quality in modern agricultural conditions, including cultivation of crops such as winter peas. One of the factors that reflects the depth of the effect of herbicides on the plant organism may be the anatomical structure of the leaf, thus, the aim of the research was to study the characteristics of epidermis and size of stipules of winter peas with the complex use of stimulator of plant growth and microbial preparation in herbicide cultivation technology. To determine the optimal combination of preparations and rates of their introduction, a field experiment was established in the Department of Biology of Uman National University of Horticulture (2018-2019), which included options: without herbicide, stimulator of plant growth and pre-sowing seed treatment with microbial preparation (control); treatment of plants with MaxiMox herbicide during the growing season in the rates of 0.8, 0.9, 1.0 and 1.1 L ha-1 separately and in mixtures with stimulator of plant growth Agriflex Amino in the rate of 1.0 kg ha-1 without and against the background of pre-sowing treatment of seeds with microbial preparation Optimize Pulse in the rate of 3.28 L t-1 (background). The experiment was repeated 3 times. Treatment of winter pea plants with preparations was carried out in the phase of 3-4 developed tendrils (BBCH 13-14). During the experiment it was found that treatment of winter pea plants with MaxiMox herbicide, especially with increasing level of the preparation to 1.1 L ha-1 led to anatomical and morphological changes in plant stipules and affected the stipule size of winter pea crops. The number of epidermal cells on average decreased by 14-53 pcs (6-22%) at LSD05 9.8 pcs, but their size increased by 28.42-394.52 μm² (2-35%) at LSD05 71.7 μm², while the size of the stipulate apparatus of crops increased on average by 2.7-4.6 thousand m² ha-1 (13-22%) at LSD05 1.3 thousand m² ha-1. The complex application of the herbicide with stimulator of plant growth, especially against the background of pre-sowing treatment of seeds with the microbial preparation Optimize Pulse in the rate of 3.28 L t-1, caused a decrease in the number of epidermal cells per unit of stipules surface on average by 50-84 pcs (21-35%) at LSD05 9.8 pcs and with an increase in the stipule size by 9-12 thousand m² ha-1 (44-59%) at LSD05 1.3 thousand m² ha-1, this may indicate the optimal effect of these mixtures of preparations on metabolic processes in plants against the background of reducing negative impact of the herbicide. [ABSTRACT FROM AUTHOR]

Common opossums (Didelphis marsupialis) are found throughout the Caribbean island of Trinidad and Tobago. The present work was conducted on the fresh normal skin of 10 common opossums and aimed to provide morphometric data and a histological description of the skin in different regions of the body. In the examined regions, the skin presented a typical thin skin morphology, with numerous folds on the surface. The dermis–epidermis junction is smooth, without the occurrence of dermal papillae. The average thickness of the opossum epidermis was 19.5 µm. The cranial region of the back showed the greatest epidermal thickness, and together with the other areas of the back presented an epidermis 2–3 times thicker compared with the other areas examined. To further confirm that the skin changes in the opossum are age‐ or diet‐related, additional studies are required. [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate anatomical comparison between six species of Euphorbia were investigated. Anatomically it has been shown that the shape of stem, midrib, lamina and margin were different. There were druses and tannins in lamina of Euphorbia microsphaera and absent in another species. The trichomes in stems were unicellular non-glandular and glandular in Euphorbia petiolate, Euphorbia phymutospering and Euphorbia peplus, while unicellular glandular in Euphorbia microsphaera and Euphorbia helioscopia, and unicellular non-glandular as in Euphorbia macroclada. In the leaf the trichomes were non-glandular and glandular unicellular in Euphorbia petiolate, while glandular unicellular in Euphorbia phymutosperin, Euphorbia microsphaeraand Euphorbia helioscopia. Secretory canal was presence in all species. The anticlinal surfaces of epidermis straight or wavy, the stomata were anisocytic, anomocytic, paracytic and hemi-paracytic with presence contiguous stomata. The pollen grains were different in size and shape such as spherical, oblate-prolate, sub-oblate and prolate spheroidal; the sculpture was foveolate or reticulate. [ABSTRACT FROM AUTHOR]

The ability to directly harvest thin and superthin perforator flaps without jeopardizing their vascularity depends on knowledge of the microsurgical vascular anatomy of each perforator within the subcutaneous tissue up to the dermis. In this paper, we report our experience with ultrahigh-frequency ultrasound (UHF-US) in the preoperative planning of thin and superthin flaps. Between May 2017 and September 2018, perforators of seven patients were preoperatively evaluated by both ultrasound (using an 18-MHz linear probe) and UHF-US (using 48- and 70-MHz linear probes). Thin flaps (two cases) and superthin flaps (five cases) were elevated for the reconstruction of head and neck oncologic defects and lower limb traumatic defects. The mean flap size was 6.5×15 cm (range, 5×8 to 7.5×23 cm). No complications occurred, and all flaps survived completely. In all cases, we found 100% agreement between the preoperative UHF-US results and the intraoperative findings. The final reconstructive outcomes were considered satisfactory by both the surgeon and the patients. In conclusion, UHF-US was found to be very useful in the preoperative planning of thin and superthin free flaps, as it allows precise anticipation of very superficial microvascular anatomy. UHF-US may represent the next frontier in thin, superthin, and pure skin perforator flap design. [ABSTRACT FROM AUTHOR]

In the study, Puschkinia scilloides Adams, P. bilgineri Yıldırım, and P. peshmenii Rix et B. Mathew were compared anatomically. Although the stem vascular bundles were arranged in two rows in P. peshmenii, they can observed in three rows in P. scilloides and P. bilgineri. In addition, some differences were found, such as the metaxylem number in the root, number of vascular bundles in the scape, presence of raphide crystals, and number of protruding epidermis cells at the tip of the leaf. These differences have been found to be taxonomically useful for distinguishing these species. Moreover, similarities and differences between Puschkinia Adams and the taxonomically closely related Scilla L. section Chionodoxa Boiss. and Scilla bifolia L. taxa were also discussed. [ABSTRACT FROM AUTHOR]

The current study aims to study the anatomical characteristic of epidermal cells, indumentum and cross sections of stems, leaves, petioles, fruitsand seedsof Corchorusolitorius from Malvaceae family, which collected from the herbal garden of AL-Rashidia city north of Baghdad. The anatomical study showed important characteristics can assessing to recognition the morphological characteristics which can be used to cultivated and increase the Corchorusolitoriuscrop in Iraq because of its high medical and nutritional importance of this species. The most important characteristics identified during the process of studying cross sections of plant parts are the stem is circular shape consists from uniseriate epidermis covered by cuticle and the cortex in addition the vascular bundles consist of xylem and phloem, As for the leaf epidermis appear stomata anisocyticshape and many glandular trichomes diffuse in the surface of epidermis, the head of this trichomes consist from multicellular cells, this is an important taxonomic characteristic for distinguishing between species within one genus and the upper epidermisconsist from large vacuole alsothe mesophyll showed numerous prismatic crystals diffuse in the cells, the midrib of leaf showed that the shape of the whole section like flask shape and from the upper side the upper epidermis and part of cortex constitute special shape like as the camel hump and under it located big cavity or known specialized schizogenous intercellular spacesalso this characters considered special taxonomic feature to distinguished between the species, the vascular bundle central located under the cavitycrescent shape include xylem and phloem. The cross sections of petiole horseshoeand many unicellular and uniseriatetrichomes spreader on the epidermis of it. [ABSTRACT FROM AUTHOR]

The stereotyped floral morphology of Neotropical Malpighiaceae species—zygomorphic and calyx with oil glands—is correlated with oil-bee pollination. In contrast, the floral trends of the Paleotropical lineages are actinomorphy and lack of calyx glands, probably due to the absence of oil-collecting bees. The Paleotropical genus Acridocarpus is an exception because of its zygomorphic, gland-bearing flowers. Glands throughout Acridocarpus inflorescences were morphoanatomically evaluated to verify the occurrence of patterns related to species and geographic distribution. Herbarium (25 species) and fresh samples of A. longifolius were processed according to standard anatomical techniques. To verify the presence of glucose and protein, strip tests were applied to the exudate. Fresh samples were fixed and submitted to histochemical tests. Based on the occurrence, number and placement of glands, three distribution patterns were recognized on the bracteole and ten on the calyx. Bract, bracteole and sepal glands have a typical nectary structure with a palisade-like epidermis and vascularized parenchyma. Glands were classified as short-stalked, sessile or immersed. Histochemical tests performed on bract and sepal glands of A. longifolius were positive for proteins, polysaccharides and phenolic compounds, and negative for oil compounds. Glucose and protein were detected in the exudate. These results allow us to recognize the glands in Acridocarpus inflorescences as nectaries. This comprehensive morphoanatomical study helps to clarify the correlation between patterns of floral morphology and secretion consumers, as well as to better understand floral evolution in Malpighiaceae across their dispersal events. [ABSTRACT FROM AUTHOR]

Migration of cells from both local and systemic sources is essential for the inflammatory and regenerative processes that occur during normal wound healing. CXCL12 is considered a critical regulator of CXCR4-positive cell migration during tissue regeneration. In this study, we investigated the expression of Cxcl12 and Cxcr4 during healing of a murine full thickness ear wound. We also investigated the expression of angiopoietin-like 4, which has been shown to participate in wound angiogenesis and reepithelialization. At time points up to 48hrs, complete blood counts were performed using automated hematology analysis, and the numbers of circulating stem and progenitor cells quantified using flow cytometry. Expression of both Cxcr4 and Angptl4 was significantly elevated within 3 days of wounding, and both were strongly expressed in cells of the epidermis. ANGPTL4 protein expression remained elevated in the epithelium through day 14. Cxcl12 expression was increased significantly at day 3, and remained elevated through day 21. Faint Cxcl12 staining was detectable in the epithelium at day 1, and thereafter staining was faint and more generalized. There were significantly fewer circulating total white blood cells and lymphocytes 1hr following ear punching. Similarly, there was a significant early (1hr) reduction in the number of circulating endothelial progenitor cells. Further studies are warranted to investigate whether ANGPTL4 and CXCL12/CXCR4 interact or synergize to facilitate cell recruitment and migration, and to potentiate reepithelialization and wound healing. [ABSTRACT FROM AUTHOR]

Telomere shortening is associated with aging and age-associated diseases. Additionally, telomere dysfunction resulting from telomerase gene mutation can lead to premature aging, such as apparent skin atrophy and hair loss. However, the molecular signaling linking telomere dysfunction to skin atrophy remains elusive. Here we show that dysfunctional telomere disrupts BMP/pSmad/P63 signaling, impairing epidermal stem cell specification and differentiation of skin and hair follicles. We find that telomere shortening mediated by Terc loss up-regulates Follistatin (Fst), inhibiting pSmad signaling and down-regulating P63 and epidermal keratins in an ESC differentiation model as well as in adult development of telomere-shortened mice. Mechanistically, short telomeres disrupt PRC2/H3K27me3-mediated repression of Fst. Our findings reveal that skin atrophy due to telomere dysfunction is caused by a previously unappreciated link with Fst and BMP signaling that could be explored in the development of therapies. [ABSTRACT FROM AUTHOR]

The stem anatomy of Turbinicarpus s.l. was studied with the aims of finding characters to support the three clades (Rapicactus, Kadenicarpus, and Turbinicarpus) in which Turbinicarpus s.l. was recovered in the most recent phylogeny of the Cacteae tribe. Thirty-five taxa were prepared, and their tissues were compared. Substantial variation was found in the epidermal surface. The hypodermis has concentric druses (Rapicactus clade) or prismatic crystals (Kadenicarpus and Turbinicarpus clades) in the cell lumina. There are abundant collateral cortical bundles, but they are amphicribal in a few taxa, and xylary fibers occur in the Kadenicarpus clade. All members of Turbinicarpus s.l. have phloem without sclerenchyma and nonfibrous wood, except for T. subterraneus, which has wood with few fibers. The periderm has an epidermal origin, and the phellem may have thin-walled cells or alternating thin- to thick-walled layers. Our results support the three clades. The Kadenicarpus clade comprises the species with xylary fibers in cortical bundles, but it shares prismatic crystals in the hypodermis, thin-walled phellem cells and partially dilated rays with the Turbinicarpus clade. The members of the Rapicactus clade have concentric druses in the hypodermis. The anatomical features proved to be valuable to support the recognition of monophyletic clades. [ABSTRACT FROM AUTHOR]

The light-promoted recovery of epidermal barrier of skin was evaluated by the associated recovery of transepidermal potential (TEP), the potential difference between the surface and dermis of skin, by using porcine skin samples. An accelerated recovery of TEP was observed by irradiation of red light with the irradiance of 40 mW/cm2 and a duration of > 10 min. The influence of the light stimulation to the surroundings (~ 20 mm) was also observed. The irradiations of blue and purple lights were ineffective in accelerating the barrier recovery. These characteristics of the light stimulation would be useful for the design of effective and safe phototherapy devices for skin. The present study proves that the TEP can serve as a spatiotemporal indicator of the epidermal barrier function. [ABSTRACT FROM AUTHOR]

Although Mycobacterium leprae (M.leprae) is usually found in macrophages and nerves of the dermis of patients with multibacillary leprosy, it is also present in all layers of the epidermis, basal, suprabasal, prickle cells, and keratin layers. However, the mechanism by which M.leprae invades the dermis remains unknown, whereas the underlying mechanism by which M.leprae invades peripheral nerves, especially Schwann cells, is well defined. M. leprae binds to the α-dystroglycan (DG) of Schwann cells via the interaction of α-DG and laminin (LN) -α2 in the basal lamina, thus permitting it to become attached to and invade peripheral nerves. In the current study, we investigated the issue of how M.leprae is phagocytosed by human epidermal keratinocytes, neonatal (HEKn). LN-5 is the predominant form of laminin in the epidermis and allows the epidermis to be stably attached to the dermis via its interaction with α/β-DG as well as integrins that are produced by keratinocytes. We therefore focused on the role of LN-5 when M. leprae is internalized by HEKn cells. Our results show that M.leprae preferentially binds to LN-5-coated slides and this binding to LN-5 enhances its binding to HEKn cells. The findings also show that pre-treatment with an antibody against α-DG, integrin-β1, or -β4 inhibited the binding of LN-5-coated M.leprae to HEKn cells. These results suggest that M. leprae binds to keratinocytes by taking advantage of the interaction of LN-5 in the basal lamina of the epidermis and a surface receptor of keratinocytes, such as α-DG, integrin-β1, or -β4. [ABSTRACT FROM AUTHOR]

p63 is a transcriptional regulator of ectodermal development that is required for basal cell proliferation and stem cell maintenance. p73 is a closely related p53 family member that is expressed in select p63-positive basal cells and can heterodimerize with p63. p73-/- mice lack multiciliated cells and have reduced numbers of basal epithelial cells in select tissues; however, the role of p73 in basal epithelial cells is unknown. Herein, we show that p73-deficient mice exhibit delayed wound healing despite morphologically normal-appearing skin. The delay in wound healing is accompanied by decreased proliferation and increased levels of biomarkers of the DNA damage response in basal keratinocytes at the epidermal wound edge. In wild-type mice, this same cell population exhibited increased p73 expression after wounding. Analyzing single-cell transcriptomic data, we found that p73 was expressed by epidermal and hair follicle stem cells, cell types required for wound healing. Moreover, we discovered that p73 isoforms expressed in the skin (ΔNp73) enhance p63-mediated expression of keratinocyte genes during cellular reprogramming from a mesenchymal to basal keratinocyte-like cell. We identified a set of 44 genes directly or indirectly regulated by ΔNp73 that are involved in skin development, cell junctions, cornification, proliferation, and wound healing. Our results establish a role for p73 in cutaneous wound healing through regulation of basal keratinocyte function. [ABSTRACT FROM AUTHOR]

Introduction. The aim of this research was to test, in an animal model, the nerve regeneration technique with a hypoallergenic acellular dermal matrix used to wrap the microsurgical neural suture. Materials and Methods. Two groups of rats received the cut of limb right median nerves. The regeneration technique considers for both groups an end-to-end nerve suture. In the experimental group (A) was used also a wrapping protocol by a conduit of collagen matrix currently used in oral surgery. The animals underwent functional grasping tests (at 1, 3, 5, and 7 months) and a histological and quantitative analysis of distal nerve was performed at the end of experimental time. Result. After seven months, the grasping test reveals functional recovery in each tested animal; this improvement is more evident in Group A. The fibers appear well organized with restored myelin sheaths in both groups. Group A showed a great quantity of connective tissue surrounding the nerve. The quantitative morphology analysis in both groups shows a similar fibers density, fiber diameter, and myelin thickness. The differences between the groups in axon mean diameter are significant. In Group A M/d, D/d, and g-ratio is significantly higher compared to control group. Conclusions. Histological and functional assessments show a functional recovery of the injured nerve in the test groups, stressed by the results of the grasping tests and the meaningful increasing in fiber diameter and higher g-ratio. Moreover, a connective tissue cuff distinguishes the distal portion of the injured nerve. Considering the easy availability and handling of the material used in this study we can conclude that this experimental technique can be considered as a valid alternative to protect nerves in nerve wrap surgery. [ABSTRACT FROM AUTHOR]

Background and aims: Some species of Cheilanthoid ferns are characterized by show adaptations to xeric habitats. Furthermore, presents the peculiarity of the roll their blades under drought stress and when conditions are optimal they expand again. The aims of this work are provide morpho-anatomical information of the leaves and relate with the environmental conditions in which grow. M&M: The fronds' morpho-anatomy of four species (Adiantopsis radiata, Cheilanthes glauca, Doryopteris concolor and D. pentagona) were analyzed through light microscopy. Also, the stomatal density and stomatal index were obtained. Results: Homogenous mesophyll was observed in D. pentagona. Adiantopsis radiata, C. glauca and D. concolor showed dorsiventral heterogeneous mesophyll. All species studied showed hypostomatic leaf, with anomocytic stomata. The stomatal index observed was between 9.1% (A. radiata) and 14.1% (D. concolor), and the range of stomata density varied between 39.7 (D. pentagona) and 57.8 (D. concolor). Conclusions: The mesophyll observed in A. radiata, and D. pentagona responds to a mesophytic environment. Doryopteris concolor was reported as a facultative epiphyte, and its dorsiventral heterogeneous mesophyll would be an advantage in this habitat. Cheilanthes glauca has xeromorphic and mesomorphic features, probably resulting from water availability during the different seasons. We porpose as hypothesis that the thickening of the anticlinal walls of epidermal cells could be involved in the leaf movements. [ABSTRACT FROM AUTHOR]

Growth differentiation factor 11 (GDF11) belongs to the TGF-β superfamily of proteins and is closely related to myostatin. Recent findings show that GDF11 has rejuvenating properties with pronounced effects on the cardiovascular system, brain, skeletal muscle, and skeleton in mice. Several human studies were also conducted, some implicating decreasing levels of circulating GDF11 with age. To date, however, there have not been any reports on its role in human skin. This study examined the impact of GDF11 on human skin, specifically related to skin aging. The effect of recombinant GDF11 on the function of various skin cells was examined in human epidermal keratinocytes, dermal fibroblasts, melanocytes, dermal microvascular endothelial cells and 3D skin equivalents, as well as in ex vivo human skin explants. GDF11 had significant effects on the production of dermal matrix components in multiple skin models in vitro and ex vivo. In addition, it had a pronounced effect on expression of multiple skin related genes in full thickness 3D skin equivalents. This work, for the first time, demonstrates an important role for GDF11 in skin biology and a potential impact on skin health and aging. [ABSTRACT FROM AUTHOR]

The root, trunk and leaf anatomy of four taxa of Scutellaria albida namely, S. albida subsp. albida, S. albida subsp. velenovskyi, S. albida subsp. colchica and S. albida subsp. condensata were examined. The aim is to determine whether these characters can be used for systematic purposes. The roots displays a typical stele structure in all studied taxa. But their number of pith rays are different in each taxa. The stems of S. albida taxa have square like transection, collateral vascular bundles, parenchymatous pith and show 1-2 layered epidermis coated with thick cuticle. S. albida subsp. condensata has papillate epicuticular wax on its stem cuticle layer. In all taxa angular collenchyma, and cambium forming phloem outward and xylem inward are observed. The leaves are hypostomatic, have single rowed epidermis coated with thick cuticle and show dorsiventral mesophyll in all studied taxa. S. albida subsp. velenovskyi has echinate epicuticular wax on its leaf cuticle layer, but the others have smooth cuticle layer. The midrib shows one collateral bundles in all taxa but the shape of median veins of the leaf blade show variation in each taxa. Some of the anatomical characters viz. the number of pith rays of roots, the number of palisade parenchyma layer, plant cuticles covered by wax, the shape of median veins of the leaf blade and stomata index, provide information of taxonomical significance for these taxa. [ABSTRACT FROM AUTHOR]

Background and aims: Some species of Cheilanthoid ferns are characterized by show adaptations to xeric habitats. Furthermore, presents the peculiarity of the roll their blades under drought stress and when conditions are optimal they expand again. The aims of this work are provide morpho-anatomical information of the leaves and relate with the environmental conditions in which grow. M&M: The fronds' morpho-anatomy of four species (Adiantopsis radiata, Cheilanthes glauca, Doryopteris concolor and D. pentagona) were analyzed through light microscopy. Also, the stomatal density and stomatal index were obtained. Results: Homogenous mesophyll was observed in D. pentagona. Adiantopsis radiata, C. glauca and D. concolor showed dorsiventral heterogeneous mesophyll. All species studied showed hypostomatic leaf, with anomocytic stomata. The stomatal index observed was between 9.1% (A. radiata) and 14.1% (D. concolor), and the range of stomata density varied between 39.7 (D. pentagona) and 57.8 (D. concolor). Conclusions: The mesophyll observed in A. radiata, and D. pentagona responds to a mesophytic environment. Doryopteris concolor was reported as a facultative epiphyte, and its dorsiventral heterogeneous mesophyll would be an advantage in this habitat. Cheilanthes glauca has xeromorphic and mesomorphic features, probably resulting from water availability during the different seasons. We porpose as hypothesis that the thickening of the anticlinal walls of epidermal cells could be involved in the leaf movements. [ABSTRACT FROM AUTHOR]

In vivo and ex vivo sensors have the potential to aid tracking and anti-poaching endeavours and provide new insights into rhinoceros physiology and environment. However, the propagation of electromagnetic signals in rhinoceros tissue is currently not known. We present simulation and agar models of the rhinoceros that allow the investigation of electromagnetic propagation by in vivo and ex vivo devices without the need for surgery. Since the dielectric properties of rhinoceros tissue have not been documented, the conductivity and permittivity of the skin, fat, muscle, blood and other organs are first approximated by means of a meta-analysis that includes animals with similar physical properties. Subsequently, we develop anatomical models that include dermal layers, internal organs and a skeleton. We also develop a flank model that serves as an approximation of the anatomical model in certain situations. These models are used to determine the viability of communication between an in vivo device and an ex vivo device attached to the hind leg of the animal. Two types of antenna (microstrip-fed planar elliptical monopole antenna and printed inverted-F antenna) and three feasible implant locations (back, neck and chest) are considered. In addition to the computer models, phantom recipes using salt, sugar and agar are developed to match the dielectric properties of each tissue type at the industrial, scientific and medical (ISM) frequencies of 403MHz, 910MHz and 2.4GHz. The average error between the measured and theoretically predicted dielectric values was 6.22% over all recipes and 4.49% for the 2.4 GHz recipe specifically. When considering the predicted efficiency of the transmitting and receiving antennas, an agreement of 67.38% was demonstrated between the computer simulations and laboratory measurements using the agar rhinoceros flank models. Computer simulations using the anatomical model of the rhinoceros indicate that the chest is the optimal implant location and that best signal propagation is achieved using the planar inverted-F antenna (PIFA). Using this configuration, the simulations indicate that communication between the implant and an ex vivo device attached to the hind leg is challenging but possible. Furthermore, we find that the inclusion of factors such as the density and temperature of the phantom materials were found to be critical to the achievement of good agreement between practice and simulation. [ABSTRACT FROM AUTHOR]

Delayed wound healing and reduced barrier function with an increased risk of cancer are characteristics of aged skin and one possible mechanism is misregulation or dysfunction of epidermal stem cells during aging. Recent studies have identified heterogeneous stem cell populations within the mouse interfollicular epidermis that are defined by territorial distribution and cell division frequency; however, it is unknown whether the individual stem cell populations undergo distinct aging processes. Here we provide comprehensive characterization of age-related changes in the mouse epidermis within the specific territories of slow-cycling and fast-dividing stem cells using old wild-type, senescence-accelerated mouse prone 1 (SAMP1) and SAMP8 mice. During aging, the epidermis exhibits structural changes such as irregular micro-undulations and overall thinning of the tissue. We also find that, in the old epidermis, proliferation is preferentially decreased in the region where fast-dividing stem cells reside whereas the lineage differentiation marker appears to be more affected in the slow-cycling stem cell region. Furthermore, SAMP8, but not SAMP1, exhibits precocious aging similar to that of aged wild-type mice, suggesting a potential use of this model for aging study of the epidermis and its stem cells. Taken together, our study reveals distinct aging processes governing the two epidermal stem cell populations and suggests a potential mechanism in differential responses of compartmentalized stem cells and their niches to aging. [ABSTRACT FROM AUTHOR]

Objectives: Soft tissue wounds with exposed bone often require extended healing times and can be associated with severe complications. We describe the ability of artificial dermis with autogenic adipose-derived stem cells (ADSCs) to promote the healing of wounds with exposed bone in a rat model. Methods: Adipose tissues harvested from the bilateral inguinal regions of Wistar rats were used as ADSCs. Rats were randomly divided into control and ADSC groups to investigate the efficacy of ADSC transplantation for wound healing (n = 20 per group). Soft tissue defects were created on the heads of the rats and were covered with artificial dermis with or without the seeded ADSCs. Specimens from these rats were evaluated using digital image analysis, histology, immunohistochemistry, cell labeling, and real-time reverse-transcription polymerase chain reaction (real-time RT-PCR). Results: The average global wound area was significantly smaller in the ADSC group than in the control group on days 3, 7, and 14 after surgery (p<0.05). After 14 days, the blood vessel density in the wound increased by 1.6-fold in the ADSC group compared with that in the control group (p<0.01). Real-time RT-PCR results showed higher Fgfb and Vegf expression levels at all time points, and higher Tgfb1 and Tgfb3 expression levels until 14 days after surgery in the ADSC group than in the control group (p<0.05). Conclusions: In wounds with exposed bone, autogenic ADSCs can promote vascularization and wound healing. Use of this cell source has multiple benefits, including convenient clinical application and lack of ethical concerns. [ABSTRACT FROM AUTHOR]

In this research, anatomical, leaf micromorphological features of the samples belonging to 25 taxa (Anacamptis Rich., Cephalanthera Rich., Dactylorhiza Necker ex Nevski, Gymnadenia R.Br., Himantoglossum Spreng., Limodorum Boehm., Ophrys L., Orchis L., Platanthera Rich., Serapias L., Spiranthes Rich. and Steveniella Schltr.) spread in the Karadeniz Region have been evaluated comparatively. In anatomical studies, the transverse section from root, stem and leaf, and surface section from leaves of plants were examined. In addition, micromorphological properties of leaf were determined by electron microscopy. Morphometrical analyses were carried out using the anatomical and leaf micromophological characters of each taxa. The data matrices were obtained by examining the results of at least three samples collected from different localities. The data were evaluated using Statistical Package for the Social Sciences (SPSS) and PAleontological STatistics (PAST) statistical programs with PCA, linear discriminant analysis (LDA), and unweighted pair group method with arithmetic mean analysis. Anatomical characteristics of plants such as root epidermis cell length, cortex diameter and pith cell diameter, leaf upper epidermis length–width and bulliform cell length–width were determined to be important characteristics. It was concluded that these characters are especially important in grouping at the genus level. [ABSTRACT FROM AUTHOR]

Comparative morphological and anatomical studies of stem, leaf, and petiole of the three common Boerhavia species found in Nigeria were carried out to determine the taxonomic significance of these characters. No clear intraspecific variation was observed among the studied species on the basis of their morphological attributes and trichome types. However, the epidermal and anatomical features were diagnostic. Differences in the stomata type made the species distinct. Also, the occurrence of raphide in their leaf lamina, the number of vascular bundles in midrib and stem, and the presence, shape, and clustering of macrosclereids in the leaf epidermis varied from one species to another. [ABSTRACT FROM AUTHOR]

There is a pressing need for improved preclinical model systems in which to study human skin wound healing. Here, we report the development and application of a serum-free full thickness human skin wound healing model. Not only can re-epithelialization (epidermal repair) and angiogenesis be studied in this simple and instructive model, but the model can also be used to identify clinically relevant wound-healing promoting agents, and to dissect underlying candidate mechanisms of action in the target tissue. We present preliminary ex vivo data to suggest that Thyroxine (T4), which reportedly promotes skin wound healing in rodents in vivo, may promote key features of human skin wound healing. Namely, T4 stimulates re-epithelialisation and angiogenesis, and modulates both wound healing-associated epidermal keratin expression and energy metabolism in experimentally wound human skin. Functionally, the wound healing-promoting effects of T4 are at least partially mediated via fibroblast growth factor/fibroblast growth factor receptor-mediated signalling, since they could be significantly antagonized by bFGF-neutralizing antibody. Thus, this pragmatic, easy-to-use full-thickness human skin wound healing model provides a useful preclinical research tool in the search for clinically relevant candidate wound healing-promoting agents. These ex vivo data encourage further pre-clinical testing of topical T4 as a cost-efficient, novel agent in the management of chronic human skin wounds. [ABSTRACT FROM AUTHOR]

Differentiation of secretory cells leads to sharp increases in protein synthesis, challenging endoplasmic reticulum (ER) proteostasis. Anticipatory activation of the unfolded protein response (UPR) prepares cells for the onset of secretory function by expanding the ER size and folding capacity. How cells ensure that the repertoire of induced chaperones matches their postdifferentiation folding needs is not well understood. We find that during differentiation of stem-like seam cells, a typical UPR target, the Caenorhabditis elegans immunoglobulin heavy chain-binding protein (BiP) homologue Heat-Shock Protein 4 (HSP-4), is selectively induced in alae-secreting daughter cells but is repressed in hypodermal daughter cells. Surprisingly, this lineage-dependent induction bypasses the requirement for UPR signaling. Instead, its induction in alae-secreting cells is controlled by a specific developmental program, while its repression in the hypodermal-fated cells requires a transcriptional regulator B-Lymphocyte–Induced Maturation Protein 1 (BLMP-1/BLIMP1), involved in differentiation of mammalian secretory cells. The HSP-4 induction is anticipatory and is required for the integrity of secreted alae. Thus, differentiation programs can directly control a broad-specificity chaperone that is normally stress dependent to ensure the integrity of secreted proteins. [ABSTRACT FROM AUTHOR]

Engineered skin substitutes (ESS), prepared using primary human fibroblasts and keratinocytes with a biopolymer scaffold, were shown to provide stable closure of excised burns, but relatively little is known about innervation of ESS after grafting. This study investigated innervation of ESS and, specifically, whether Merkel cells are present in healed grafts. Merkel cells are specialized neuroendocrine cells required for fine touch sensation in skin. We discovered cells positive for keratin 20 (KRT20), a general marker for Merkel cells, in the basal epidermis of ESS after transplantation to mice, suggesting the presence of Merkel cells. Cells expressing KRT20 were not observed in ESS in vitro. However, widely separated KRT20-positive cells were observed in basal epidermis of ESS by 2 weeks after grafting. By 4 weeks, these cells increased in number and expressed keratins 18 and 19, additional Merkel cells markers. Putative Merkel cell numbers increased further between weeks 6 and 14; their densities varied widely and no specific pattern of organization was observed, similar to Merkel cell localization in human skin. KRT20-positive cells co-expressed epidermal markers E-cadherin and keratin 15, suggesting derivation from the epidermal lineage, and neuroendocrine markers synaptophysin and chromogranin A, consistent with their identification as Merkel cells. By 4 weeks after grafting, some Merkel cells in engineered skin were associated with immature afferents expressing neurofilament-medium. By 8 weeks, Merkel cells were complexed with more mature neurons expressing neurofilament-heavy. Positive staining for human leukocyte antigen demonstrated that the Merkel cells in ESS were derived from grafted human cells. The results identify, for the first time, Merkel cell-neurite complexes in engineered skin in vivo. This suggests that fine touch sensation may be restored in ESS after grafting, although this must be confirmed with future functional studies. [ABSTRACT FROM AUTHOR]

The anatomical comparative study of petiole and pinnule cross-sections of nine species from Plagiogyria was performed using light microscopy for the first time. The results showed that the epidermis, the basic tissue and middle column of middle petiole transection and epidermis, mesophyll and main vein of the vegetative leaf pinnule of nine species of Plagiogyria were similar, for example, the epidermis of the transverse section of the middle petiole was not covered with hair or squamas, the epidermis was round in shape with thick-walled tissue, and the central cylinder was a amphicribral vascular bundle; the stomatas of trophyll pinnule transection were only distributed in the lower epidermis, the shape of epidermal cells was flat, and the type of the main vein belongs to the peripheral vascular bundle, which supports for the viewpoint that Plagiogyria was a natural taxonomy. In the evolution of the system, Plagiogyria had a certain relationship with the Alsophila spinulosa and both of them had similar characteristics and also showed certain differences. However, the petiole central transverse section of these nine species of Plagiogyria were trapezoidal, oval or triangular. The shape of the vascular bundle including “U” shape, “V” shape or triangle. The number of vascular bundles in the central transverse section of the petiole was one or three. The arrangement of xylem in vascular bundles including Chinese character “Eight” shape, “U” shape or “-” shape; The underside protuberances of the pinnule main veins were curved, triangular or trapezoidal. The xylem of the petiole had two forms: typical hippocampus and atypical hippocampus. The typical hippocampus was characterized by obvious hook state at both ends of the xylem. The atypical hippocampus was characterized by no obvious hook state at the two ends of xylem or one of the xylems had no hook at one end. These morphological anatomical features are stable and cluster specific, which provides a new reference for taxonomy and systematic studies of Plagiogyria. Finally, a key to the Plagiogyria species is given based on the anatomical characteristics of the transverse section of petiole and pinnule. [ABSTRACT FROM AUTHOR]

We developed a novel skin regeneration therapy combining nevus tissue inactivated by high hydrostatic pressure (HHP) in the reconstruction of the dermis with a cultured epidermal autograft (CEA). The issue with this treatment is the unstable survival of CEA on the inactivated dermis. In this study, we applied collagen/gelatin sponge (CGS), which can sustain the release of basic fibroblast growth factor (bFGF), to the inactivated skin in order to accelerate angiogenesis. Murine skin grafts from C57BL6J/Jcl mice (8 mm in diameter) were prepared, inactivated by HHP and cryopreserved. One month later, the grafts were transplanted subcutaneously onto the back of other mice and covered by CGS impregnated with saline or bFGF. Grafts were harvested after one, two and eight weeks, at which point the engraftment was evaluated through the histology and angiogenesis-related gene expressions were determined by real-time polymerase chain reaction. Histological sections showed that the dermal cellular density and newly formed capillaries in the bFGF group were significantly higher than in the control group. The relative expression of FGF-2, PDGF-A and VEGF-A genes in the bFGF group was significantly higher than in the control group at Week 1. This study suggested that the angiogenesis into grafts was accelerated, which might improve the engraftment of inactivated dermis in combination with the sustained release of bFGF by CGSs. [ABSTRACT FROM AUTHOR]

Changes in leaf anatomy and ultrastructure are associated with physiological performance in the context of plant adaptations to climate change. In this study, we investigated the isolated and combined effects of elevated atmospheric CO2 concentration ([CO2]) up to 600 μmol mol-1 (eC) and elevated temperature (eT) to 2°C more than the ambient canopy temperature on the ultrastructure, leaf anatomy, and physiology of Panicum maximum Jacq. grown under field conditions using combined free-air carbon dioxide enrichment (FACE) and temperature free-air controlled enhancement (T-FACE) systems. Plants grown under eC showed reduced stomatal density, stomatal index, stomatal conductance (gs), and leaf transpiration rate (E), increased soil-water content (SWC) conservation and adaxial epidermis thickness were also observed. The net photosynthesis rate (A) and intrinsic water-use efficiency (iWUE) were enhanced by 25% and 71%, respectively, with a concomitant increase in the size of starch grains in bundle sheath cells. Under air warming, we observed an increase in the thickness of the adaxial cuticle and a decrease in the leaf thickness, size of vascular bundles and bulliform cells, and starch content. Under eCeT, air warming offset the eC effects on SWC and E, and no interactions between [CO2] and temperature for leaf anatomy were observed. Elevated [CO2] exerted more effects on external characteristics, such as the epidermis anatomy and leaf gas exchange, while air warming affected mainly the leaf structure. We conclude that differential anatomical and physiological adjustments contributed to the acclimation of P. maximum growing under elevated [CO2] and air warming, improving the leaf biomass production under these conditions. [ABSTRACT FROM AUTHOR]