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As a common food processing technology, microbial fermentation is becoming increasingly popular to promote the bioactivity of materials. This study aims to enhance rape bee pollen bioactivity through fermentation and trace the potential components associated with its bioactivity. The antioxidant and anti-inflammatory activities of unfermented bee pollen and fermented bee pollen were evaluated, and their correlation with differential metabolites was analyzed. The results indicated that fermentation significantly (p < 0.05) improved the antioxidant (>2.3-fold) and anti-inflammatory (>1.36-fold) activities of bee pollen, and increased the contents of total phenolics and flavonoids by 1.99 and 1.53 folds. Moreover, the correlation analysis results indicated that 15 components, including three phenolamides, one flavonoid aglycone, seven fatty acids, three amino acids and one ketone compound, were positively correlated with bee pollen antioxidant and anti-inflammatory activities. These results suggest that fermentation is a promising approach to increase the bioactivity of bee pollen., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The phytochemical contents, peroxyl radical scavenging capacities (PSCs) and cellular antioxidant activities (CAAs) of free and bound fractions of rice were reported. Black rice had the highest total phenolic content and total flavonoid content in free and bound fractions, followed by red rice, brown rice, and polished rice. Black rice contained much more free phenolic compounds than other rice samples, such as cyanidin-3-O-glucoside, protocatechuic acid, and vanillic acid. Tocopherols and tocotrienols contents were highest in red rice, then in black rice, brown rice, and polished rice. PSCs and CAAs of free and bound fractions were in the order: black rice > red rice > brown rice > polished rice, except that bound CAA of red rice was higher than that of black rice. The cellular uptake rate of free phenolics was highest in red rice, while cellular uptake rates of bound phenolics were highest in brown rice and polished rice., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Phytochemical profiles, antioxidant and antiproliferative activities of berry extracts were evaluated and compared in four subspecies of Sea buckthorn (Hippophaë rhamnoides L.). Among the subspecies, Hippophaë rhamnoides L. subsp. sinensis exhibited highest total phenolics content (38.7±1.3mgGA equiv./g DW) and corresponding total antioxidant activity. Whereas maximum cellular antioxidant and antiproliferative activities were determined in Hippophaë rhamnoides L. subsp. yunnanensis. Total antioxidant activity was significantly associated to total phenolics, isorhamnetin-3-rutinoside and isorhamnetin-3-glucoside. The cellular antioxidant activity and antiproliferative activity of phytochemicals were fairly correlated to phenolic acids and flavonoid aglycones. Lower median effective dose (EC 50 ) of individual compounds against human liver cancer HepG2 cells proliferation studies confirmed the better correlation between antiproliferative activity of Sea buckthorn extracts and flavonoid aglycones, including isorhamnetin, quercetin and kaempferol., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Mango (Mangifera indica L.), also called "the king of fruits", is one of the most popular fruits in tropical regions. Pulp and peel samples of mango cultivars were analyzed to estimate total phenolic, total flavonoid and total anthocyanin contents. Phenolic acids, hydrophilic peroxyl radical scavenging capacity (hydro-PSC) and oxygen radical scavenging capacity (ORAC) in vitro were also determined. Total phenolics and flavonoid contents were found maximum in the peel of Xiao Tainang and Da Tainang cultivars, respectively, whereas Xiao Tainang also exhibited significant antioxidant capacity. Noteworthy, concentrations of gallic acid, protocatechuic acid, ferulic acid, chlorogenic acid and caffeic acids at 79.15, 64.33, 33.75, 27.19 and 13.62 mg/100 g fresh weight (FW) were quantified for Da Tainang, Xiao Tainang and of Jidan cultivars, respectively. Comparatively, a higher level of phenolics and significant antioxidant capacity in mango peel indicated that it might be useful as a functional food and value-added ingredient to promote human health.

Cellular antioxidant activity ( CAA) and inhibition of hepato-cellular carcinoma (HepG2) proliferation were evaluated for the first time in the pulp and peel of mango cultivars. Comparatively, peel had high flavonoids and tocopherols content and showed significant antioxidant activity. Among all the studied cultivars, the Xiao Tainong peel was predominant with highest fistein, mangiferin and alpha-tocopherol content and significant cellular antioxidant activity value 2986 ± 380 μmol QE/100 g FW. The HepG2 cells antiproliferation was maximum in the peel of Da Tainong and pulp of Aozhou with lowest EC50 values, 2.35 ± 0.65 (peel) and 185.4 ± 10.9 (pulp) mg mL−1, in a dose-dependent manner. Negative associations of flavonoids and tocopherol compounds with CAA and antiproliferative activity in mango confirmed synergistic, additive or antagonistic actions of phytochemicals. The current study suggests that mango peel could be used as a value added ingredient or functional food and may contribute considerably to promote consumer health. [ABSTRACT FROM AUTHOR]

A comparative assessment of the phytochemical profiles and antioxidant activities of seven cultivars of Aloe was conducted to evaluate the potential health benefits of Aloe. Aloe arborescens contained the highest levels of phenolic content, total antioxidant capacity by the oxygen radical scavenging capacity assay and cellular antioxidant activity assay. Aloe vera showed the highest levels of flavonoid content and antioxidant capacity by the peroxyl radical scavenging capacity assay. Aloe greenii had the highest CAA value with a PBS wash before adding ABAP. There were no significant differences observed between Aloe arborescens and Aloe greenii. Aloin, aloe-emodin-8-O-beta-D-glucopyranoside, catechin, epicatechin, sinapic acid and chlorogenic acid were identified in Aloe samples by the HPLC analysis. Aloin, aloe-emodin-8-O-beta-D-glucopyranoside and catechin showed strong relationships with antioxidant activity. Significant levels of aloin, aloe-emodin-8-O-beta-D-glucopyranoside and catechin were determined in Aloe greenii, Aloe vera and Aloe saponaria, respectively. [ABSTRACT FROM AUTHOR]

Flaxseed (Linum usitatissimum L.) is well known for its health benefits and chemical composition. This study aimed to evaluate the dynamic changes caused by germination in vitamin C, phenolic compounds and antioxidant activities of flaxseed. It was observed that eight-day germination of flaxseed sprouts caused increases in phytochemical contents, that is 22.1-fold increase in vitamin C, 2.67-fold increase in phenolics and 5.48-fold increase in flavonoid contents as compare to initial concentration in flaxseeds on dry weight basis. The stimulation was also noted in the three identified phenolic acid ingredients including caffeic acid, p-coumaric acid and ferulic acid. Similarly, the total PSC and ORAC values of flax sprouts at day 8 increased by 3- to 4-folds compared to nongerminated flaxseeds. It is revealed that comparatively, 8-day germination of flax sprouts leads to a significant increase in vitamin C, phenolic constituents and total antioxidant activities than those of flaxseeds. [ABSTRACT FROM AUTHOR]

Understanding the influence of processing operations such as drying/dehydration, canning, extrusion, high hydrostatic pressure, pulsed electric field, and ohmic heating on the phytochemicals of fruits, vegetables, and grains is important in retaining the health benefiting properties of these antioxidative compounds in processed food products. Most of the previous investigations in the literature on the antioxidants of fruits, vegetables, and grains have shown that food-processing operations reduced the antioxidants of the processed foods, which is also the usual consumer perception. However, in the last decade some articles in the literature reported that the evaluation of nutritional quality of processed fruits and vegetables not only depend on the quantity of vitamin C but should include analyses of other antioxidant phytochemicals and antioxidant activity. Thermal processing increased the total antioxidant activity of tomato and sweet corn. Most importantly, analysis also depends on the condition, type, and mechanism of antioxidant assays used. This review aims to provide concise information on the influence of various thermal and nonthermal food-processing operations on the stability and kinetics of health beneficial phenolic antioxidants of fruits, vegetables, and grains. [ABSTRACT FROM AUTHOR]

Ramie (Boehmeria nivea L.) is usually cultivated as a fiber crop, but it is also well known for its potential use in animal feeding with viable commercial applications. In this study, the phenolics profile as well as cellular antioxidant and antiproliferative activities were investigated in free and bound fractions of six different vegetative parts from Boehmeria nivea L. The highest total phenolic content was observed in bud (4585 ± 320 mg GAE/100 g DW), whereas root and petiole had the lowest total phenolic contents, 442.8 ± 9.8 and 630.9 ± 27.0 mg GAE/100 g DW, respectively. Likewise, phloem had the most abundant total flavonoids (2755 ± 184 mg CE/100 g DW), whereas the lowest flavonoid contents was found in root and petiole, 636.9 ± 44.2 and 797.4 ± 87.6 mg CE/100 g DW, respectively. Xylem and bud depicted remarkable antioxidant and antiproliferative activities, which could be explained by their diverse phenolic composition, especially chlorogenic acid and epicatechin. The Boehmeria nivea L. plant might be a valuable resource for high value-added phenolic compounds used in food and non-food industries. [ABSTRACT FROM AUTHOR]

This study aims to evaluate phenolics, flavonoids, total and cellular antioxidant activity (CAA), antioxidant capacity in ear sections of sweet corn. Corn cob and mature corn silk showed the highest contents of total phenolics and flavonoids (p < 0.05), respectively. Gallic acid and ferulic acid were the main free and bound phenolics in each ear part of corn, respectively (p < 0.05). Corn resident (CR) had a dominant role in total antioxidant activity and cellular antioxidant activity (CAA) (p < 0.05). Considering all the antioxidant properties, CR showed the best performance. The content of ferulic acid was correlated closely with CAA (R2 = 0.898, p < 0.05) and total antioxidant capacity (R2 = 0.971, p < 0.05), and total antioxidant capacity was also connected tightly with total phenolics (R2 = 0.886, p < 0.05). The results of this investigation were significant to enhance recognition and application of ear sections in food industry. Practical applications: Sweet corn is widely cultivated in Asia, America, and other regions for fresh or industrial food because of high sugar, dietary fiber, and vitamins. However, there is no systematic assay on ear sections of sweet corn concerning antioxidant evaluation. The study investigated phytochemical profiles and antioxidant capacity in ear sections of sweet corn systematically, and made a comprehensive comparison of them combined with correlation analysis. The results indicated that ear sections of sweet corn, not only kernels, demonstrated high antioxidant capacity. Ear sections show promising potential application in food industry. [ABSTRACT FROM AUTHOR]

The phytochemical content and antioxidant activity of eight varieties of brown rice (BR) are reported. The total phenolic contents of BR ranged from 72.45 to 120.13 mg of gallic acid equiv./100 g. The phenolics from bound fraction contributed 40.6–50.2% of the total phenolic content. The total flavonoid contents of BR ranged from 75.90 to 112.03 mg catechin equiv./100 g. The flavonoids from the bound fraction contributed 26.9–48.2% of total flavonoids. Trans -ferulic acid was the predominant phenolic acid in BR. Total trans -ferulic acid content ranged from 161.42 to 374.81 μg/100 g. The percentage of trans -ferulic acid in bound fraction ranged from 96.4% to 99.2%. Only α- and γ-tocopherols and -tocotrienols were detected in BR with α-tocopherol and γ-tocotrienol being the predominant. The total peroxyl radical scavenging capacity (PSC) of BR ranged from 18.29 to 40.33 mg vitamin C equiv./100 g. The bound fraction contributed 67.2–77.2% of total PSC. [ABSTRACT FROM AUTHOR]