1. Colloidal gold-based immunochromatographic strip assay for the rapid detection of bacitracin zinc in milk.
- Author
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Na G, Hu X, Yang J, Sun Y, Kwee S, Tang L, Xing G, Xing Y, and Zhang G
- Subjects
- Animals, Antibodies, Monoclonal immunology, Female, Immunoassay methods, Limit of Detection, Mice, Mice, Inbred BALB C, Time Factors, Bacitracin analysis, Gold Colloid chemistry, Milk chemistry
- Abstract
In this study, a murine monoclonal antibody (mAb) of 6D2-G10 against bacitracin zinc (BAC) was produced and applied to an immunochromatographic strip (ICS) for the initial detection of BAC in milk. The ICS with a cut-off value of 25 ng/mL could be perceived by the naked eye within 10 min. With the assist of the strip reader, the limit of detection (LOD) was measured as 0.82 ng/mL, the half-maximal inhibitory concentration (IC
50 ) was recorded as 3.16 ng/mL, and the linear detection range was from 0.97 to 10.30 ng/mL. The recoveries ranged from 87.7% to 96.0% with the highest coefficient of variation (CV) of 9.1% in the intra-assay and from 84.3% to 90.2% with the highest CV of 10.7% in the inter-assay. In short, the established ICS provided a serviceable analytical tool for qualitatively and quantitatively monitoring BAC in milk., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
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