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[Display omitted] • Methane yield of thermophilic digester is greater than that of mesophilic digester. • Bacterial and archaeal diversity of mesophilic digesters is more abundant. • Plant growth promotion of mesophilic biogas slurry is more prominent. • Potassium in biogas slurry is the key factor to promote plant growth. The objective of this study was to investigate the effects of mixing ratios of Chinese cabbage waste (CCW) and cow manure (CM) on methane yields, microbial community, and biogas slurry fertility during anaerobic co-digestion. Batch experiments were conducted at mesophilic and thermophilic temperatures with five different CCW/CM mixing ratios. Methane yields at mesophilic and thermophilic temperatures were 4.2–184.4 mL g−1 Volatile solids added (mL g−1 VS added) and 11.8–321.7 mL g−1 VS added , respectively. The richness and diversity of bacteria and archaea at mesophilic temperatures were higher than those at thermophilic temperatures. Compared with the unfertilized control, the dry weight of corn seedlings with the follow-up application of mesophilic or thermophilic biogas slurry increased by 12.3 %-73.4 % or 16.8 %-43.3 %, respectively. This study demonstrates that thermophilic temperatures are conducive to increasing methane yields, but mesophilic temperatures are conducive to improving the biogas slurry fertility. [ABSTRACT FROM AUTHOR]

To enhance the dewaterability of anaerobic digested sludge and to make full use of the biogas slurry. This study set up five sludge conditioning methods: polymeric ferric sulfate, polymeric aluminum chloride, cationic polyacrylamide, chitosan, and chitosan combined with rice husk powder. Their effects on the dewaterability of thermal hydrolysis-anaerobic digested sludge, bacterial community, and biogas slurry fertility were studied to find a non-toxic and non-risk dewatering technology for the environment and biogas slurry. Compared with that of the control group, moisture content, normalization capillary suction time, and specific resistance to filtration were reduced by 12.8%, 97.7%, and 82.9%, respectively. Chitosan enlarges the sludge flocs and forms complexes with proteins, disrupting the structure of the extracellular polymeric substances, thereby exposing more hydrophobic groups and reducing the hydrophilicity of the sludge. The subsequent addition of rice husk powder enhances the adsorption of hydrophilic substances and provides a stronger drainage channel for the sludge. In addition, the biogas slurry obtained by this conditioning method used as a fertilizer increased the dry weight and fresh weight of corn seedlings by 59.3% and 91.0%, respectively. And the total chlorophyll content increased by 84.6%. Pearson's correlation analysis showed that chitosan and rice husk meal had no toxic effect on the biogas slurry compared to the other three flocculants. The results showed that the combined treatment of chitosan and rice husk powder resulted in the best dewaterability. Overall, chitosan combined with rice husk powder is a green dewatering technology with great potential for anaerobic digested sludge dewatering and biogas slurry recycling. [Display omitted] • Compared with control, CS+RHP reduced SRF and NCST of sludge by 82.9% and 97.7%. • Compared with PFS, PAC, and CPAM, CS is better for plant growth promotion. • Compared with control, dry weight of corn seedling in CS+RHP increased by 59.3%. • Compared with control, total chlorophyll of seedling in CS+RHP increased by 84.6%. [ABSTRACT FROM AUTHOR]

Abstract Digestate from biogas plants managing municipal solid waste needs to be stabilized prior to final utilization or disposal. Based on the concept of urban mining, aged refuse from a closed landfill was used to treat landfill leachate, but nitrogen removal by biological denitrification was limited. The aim of this study was to use a digestate layer in bioreactors containing aged refuse to enhance the biological denitrification capacity of the aged refuse, stabilize digestate, and mitigate the ammonia emissions from digestate leaching with leachate recirculation. Six identical landfill columns filled with 0% (R0), 5% (R5), and 15% (R15) of solid digestate above aged refuse (ratios based on Total Solids) were setup and nitrified leachate was periodically fed and recirculated to the columns. The nitrate removal rate in R5 and R15 was 3.4 and 10 times higher relative to the control (no digestate added). A 31.5–35.9% increase of solid digestate biostability was confirmed by tests performed under both aerobic and anaerobic conditions. The results showed that instead of land use, the solid fraction of digestate could be utilized as an inexpensive functional layer embedded in an old landfill site to enhance the denitrification capacity and achieve digestate stabilization with minimal ammonia leaching from digestate. Graphical abstract A combined approach to manage digestate and landfill leachate treatment. Image 1 Highlights • Bioreactors packed with aged refuse and digestate were used to treat landfill leachate. • Digestate addition enhanced the denitrification capacity of aged landfill waste tenfold. • The aged refuse mitigated the amount of ammonia leaching from the solid digestate by 67.2%–84.8%. • N removal of old landfill leachate was jointly gained with digestate stabilization. [ABSTRACT FROM AUTHOR]