6 results on '"Beck-Schimmer, Beatrice"'
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2. Targeted Large-Volume Lymphocyte Removal Using Magnetic Nanoparticles in Blood Samples of Patients with Chronic Lymphocytic Leukemia: A Proof-of-Concept Study.
- Author
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Janker, Stefanie, Doswald, Simon, Schimmer, Roman R., Schanz, Urs, Stark, Wendelin J., Schläpfer, Martin, and Beck-Schimmer, Beatrice
- Subjects
ALEMTUZUMAB ,CHRONIC lymphocytic leukemia ,MAGNETIC nanoparticles ,BLOOD sampling ,B cells ,LYMPHOCYTE count - Abstract
In the past, our research group was able to successfully remove circulating tumor cells with magnetic nanoparticles. While these cancer cells are typically present in low numbers, we hypothesized that magnetic nanoparticles, besides catching single cells, are also capable of eliminating a large number of tumor cells from the blood ex vivo. This approach was tested in a small pilot study in blood samples of patients suffering from chronic lymphocytic leukemia (CLL), a mature B-cell neoplasm. Cluster of differentiation (CD) 52 is a ubiquitously expressed surface antigen on mature lymphocytes. Alemtuzumab (MabCampath
® ) is a humanized, IgG1κ, monoclonal antibody directed against CD52, which was formerly clinically approved for treating chronic lymphocytic leukemia (CLL) and therefore regarded as an ideal candidate for further tests to develop new treatment options. Alemtuzumab was bound onto carbon-coated cobalt nanoparticles. The particles were added to blood samples of CLL patients and finally removed, ideally with bound B lymphocytes, using a magnetic column. Flow cytometry quantified lymphocyte counts before, after the first, and after the second flow across the column. A mixed effects analysis was performed to evaluate removal efficiency. p < 0.05 was defined as significant. In the first patient cohort (n = 10), using a fixed nanoparticle concentration, CD19-positive B lymphocytes were reduced by 38% and by 53% after the first and the second purification steps (p = 0.002 and p = 0.005), respectively. In a second patient cohort (n = 11), the nanoparticle concentration was increased, and CD19-positive B lymphocytes were reduced by 44% (p < 0.001) with no further removal after the second purification step. In patients with a high lymphocyte count (>20 G/L), an improved efficiency of approximately 20% was observed using higher nanoparticle concentrations. A 40 to 50% reduction of B lymphocyte count using alemtuzumab-coupled carbon-coated cobalt nanoparticles is feasible, also in patients with a high lymphocyte count. A second purification step did not further increase removal. This proof-of-concept study demonstrates that such particles allow for the targeted extraction of larger amounts of cellular blood components and might offer new treatment options in the far future. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
3. Removal of Circulating Tumor Cells from Blood Samples of Cancer Patients Using Highly Magnetic Nanoparticles: A Translational Research Project
- Author
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Doswald, Simon, Herzog, Antoine F, Zeltner, Martin, Zabel, Anja, Pregernig, Andreas, Schläpfer, Martin, Siebenhüner, Alexander, Stark, Wendelin J, Beck-Schimmer, Beatrice, University of Zurich, and Beck-Schimmer, Beatrice
- Subjects
circulating tumor cells ,nanoparticles ,blood purification ,10216 Institute of Anesthesiology ,3003 Pharmaceutical Science ,Pharmaceutical Science ,610 Medicine & health - Abstract
The count of circulating tumor cells (CTCs) has been associated with a worse prognosis in different types of cancer. Perioperatively, CTCs detach due to mechanical forces. Diagnostic tools exist to detect and isolate CTCs, but no therapeutic technique is currently available to remove CTCs in vivo from unprocessed blood. The aim of this study was to design and test new magnetic nanoparticles to purify whole blood from CTCs. Novel magnetic carbon-coated cobalt (C/Co) nanoparticles conjugated with anti-epithelial cell adhesion molecule (EpCAM) antibodies were synthesized, and their antifouling and separation properties were determined. The newly developed C/Co nanoparticles showed excellent separation and antifouling properties. They efficiently removed tumor cells that were added to healthy subjects’ blood samples, through an anti-EpCAM antibody interaction. The nanoparticles did not interact with other blood components, such as lymphocytes or the coagulation system. In blood samples of carcinoma patients suffering from metastatic disease, on average, ≥68% of CTCs were removed. These nanoparticles could prompt the development of a blood purification technology, such as a dialysis-like device, to perioperatively remove CTCs from the blood of cancer patients in vivo and potentially improve their prognosis., Pharmaceutics, 14 (7)
- Published
- 2022
4. Magnetic separation-based blood purification: a promising new approach for the removal of disease-causing compounds?
- Author
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Herrmann, K., Schlegel, A. A., Graf, R., Stark, W. J., and Beck-Schimmer, Beatrice
- Subjects
MAGNETIC separation ,MOLECULES ,BACTERIA ,CYTOLOGICAL research ,MOLECULAR weights - Abstract
Recent studies report promising results regarding extracorporeal magnetic separation-based blood purification for the rapid and selective removal of disease-causing compounds from whole blood. High molecular weight compounds, bacteria and cells can be eliminated from blood within minutes, hence offering novel treatment strategies for the management of intoxications and blood stream infections. However, risks associated with incomplete particle separation and the biological consequences of particles entering circulation remain largely unclear. This article discusses the promising future of magnetic separation-based purification while keeping important safety considerations in mind. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
5. Biochemical functionality of magnetic particles as nanosensors: how far away are we to implement them into clinical practice?
- Author
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Simon Doswald, Wendelin J. Stark, Beatrice Beck-Schimmer, University of Zurich, and Beck-Schimmer, Beatrice
- Subjects
Carrier system ,Computer science ,Synthesis methods ,Magnetic nanoparticle ,3003 Pharmaceutical Science ,Contrast Media ,Medicine (miscellaneous) ,Pharmaceutical Science ,Biosensing Techniques ,Cell Separation ,Review ,02 engineering and technology ,01 natural sciences ,Applied Microbiology and Biotechnology ,Blood purification ,10052 Institute of Physiology ,Drug Delivery Systems ,Magnetite Nanoparticles ,Risk assessment ,Coating materials ,2701 Medicine (miscellaneous) ,021001 nanoscience & nanotechnology ,Clinical Practice ,lcsh:R855-855.5 ,Molecular Medicine ,0210 nano-technology ,Medical applications ,lcsh:Medical technology ,10216 Institute of Anesthesiology ,lcsh:Biotechnology ,Biomedical Engineering ,2204 Biomedical Engineering ,Nanotechnology ,610 Medicine & health ,Bioengineering ,010402 general chemistry ,Nanosensor ,lcsh:TP248.13-248.65 ,Animals ,Humans ,2402 Applied Microbiology and Biotechnology ,1502 Bioengineering ,Hyperthermia, Induced ,equipment and supplies ,0104 chemical sciences ,Nanosensors ,1313 Molecular Medicine ,Magnetic nanoparticles ,human activities - Abstract
Magnetic nanosensors have become attractive instruments for the diagnosis and treatment of different diseases. They represent an efficient carrier system in drug delivery or in transporting contrast agents. For such purposes, magnetic nanosensors are used in vivo (intracorporeal application). To remove specific compounds from blood, magnetic nanosensors act as elimination system, which represents an extracorporeal approach. This review discusses principles, advantages and risks on recent advances in the field of magnetic nanosensors. First, synthesis methods for magnetic nanosensors and possibilities for enhancement of biocompatibility with different coating materials are addressed. Then, attention is devoted to clinical applications, in which nanosensors are or may be used as carrier- and elimination systems in the near future. Finally, risk considerations and possible effects of nanomaterials are discussed when working towards clinical applications with magnetic nanosensors., Journal of Nanobiotechnology, 17, ISSN:1477-3155
- Published
- 2019
6. Magnetic separation-based blood purification: a promising new approach for the removal of disease-causing compounds?
- Author
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Andrea Schlegel, Beatrice Beck-Schimmer, Wendelin J. Stark, Inge K. Herrmann, Rolf Graf, University of Zurich, and Beck-Schimmer, Beatrice
- Subjects
Extracorporeal Circulation ,3003 Pharmaceutical Science ,Pharmaceutical Science ,Medicine (miscellaneous) ,Review ,02 engineering and technology ,Blood purification ,01 natural sciences ,Applied Microbiology and Biotechnology ,Magnetite Nanoparticles ,Whole blood ,2701 Medicine (miscellaneous) ,021001 nanoscience & nanotechnology ,3. Good health ,Pharmaceutical Preparations ,Magnetic nanoparticles ,Molecular Medicine ,Treatment strategy ,0210 nano-technology ,Blood stream ,10216 Institute of Anesthesiology ,Blood Safety ,Intoxication ,Biomedical Engineering ,Magnetic separation ,2204 Biomedical Engineering ,610 Medicine & health ,Bioengineering ,Nanotechnology ,Biology ,010402 general chemistry ,Sepsis ,Magnetics ,Particle separation ,Blood-Borne Pathogens ,medicine ,Animals ,Humans ,2402 Applied Microbiology and Biotechnology ,Toxins, Biological ,10217 Clinic for Visceral and Transplantation Surgery ,Chromatography ,1502 Bioengineering ,Interleukins ,Extracorporeal circulation ,medicine.disease ,0104 chemical sciences ,1313 Molecular Medicine - Abstract
Recent studies report promising results regarding extracorporeal magnetic separation-based blood purification for the rapid and selective removal of disease-causing compounds from whole blood. High molecular weight compounds, bacteria and cells can be eliminated from blood within minutes, hence offering novel treatment strategies for the management of intoxications and blood stream infections. However, risks associated with incomplete particle separation and the biological consequences of particles entering circulation remain largely unclear. This article discusses the promising future of magnetic separation-based purification while keeping important safety considerations in mind. ISSN:1477-3155
- Published
- 2015
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