6 results on '"Popa, Mircea Ioan"'
Search Results
2. Evaluation of the rapid carbapenem inactivation method (rCIM): a phenotypic screening test for carbapenemase-producing Enterobacteriaceae.
- Author
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Muntean MM, Muntean AA, Gauthier L, Creton E, Cotellon G, Popa MI, Bonnin RA, and Naas T
- Subjects
- Carbapenem-Resistant Enterobacteriaceae enzymology, Enterobacter cloacae growth & development, Enterobacteriaceae Infections microbiology, Nephelometry and Turbidimetry, Prospective Studies, Retrospective Studies, Sensitivity and Specificity, Time Factors, Anti-Bacterial Agents metabolism, Bacterial Proteins analysis, Bacteriological Techniques methods, Carbapenem-Resistant Enterobacteriaceae isolation & purification, Carbapenems metabolism, Mass Screening methods, beta-Lactamases analysis
- Abstract
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection., Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain. Growth of the indicator strain is monitored using a nephelometer. The performances of the rCIM were compared with the CIM and Carba NP tests using a collection of 113 well-characterized carbapenem-resistant enterobacterial isolates, including 85 carbapenemase producers and 28 non-carbapenemase producers. In addition, rCIM was compared with the Carba NP test and PCR sequencing in a prospective analysis of 101 carbapenem-resistant enterobacterial isolates addressed to the French National Reference Center for Antimicrobial Resistance in July 2017., Results and Discussion: The rCIM correctly identified 84/85 carbapenemase producers and 28/28 non-carbapenemase producers, yielding a sensitivity of 99% and a specificity of 100%, slightly higher than the CIM and Carba NP test. In the prospective validation study, the rCIM showed a sensitivity and specificity of 97% and 95%, respectively. Two cephalosporinase-hyperproducing Enterobacter cloacae gave false-positive results, whereas an IMI-17-producing Enterobacter asburiae gave a false-negative result. The result was, however, positive when the isolate was grown on selective antibiotic-containing media., Conclusions: The rCIM is a rapid (less than 3 h), cheap and accurate test for the detection of CPEs, which can be implemented in low-resource settings, making it a useful tool for microbiology laboratories.
- Published
- 2018
- Full Text
- View/download PDF
3. Optimization of the rapid carbapenem inactivation method for use with AmpC hyperproducers.
- Author
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Muntean, Mădălina Maria, Muntean, Andrei-Alexandru, Guerin, François, Cattoir, Vincent, Creton, Elodie, Cotellon, Garance, Oueslati, Saoussen, Popa, Mircea Ioan, Girlich, Delphine, Iorga, Bogdan I., Bonnin, Rémy A., and Naas, Thierry
- Subjects
SENSITIVITY & specificity (Statistics) ,CARBAPENEMS ,ANTIBIOTICS - Abstract
Objectives: Detection of carbapenemase-producing Enterobacterales (CPEs) is sometimes difficult with AmpC-hyperproducing Enterobacterales (AHEs), as they may falsely be classified as CPEs. Here, we present a rapid Carbapenem Inactivation Method (rCIM) optimized for AmpC producers (rCIM-A) that allows rapid and easy discrimination between AHEs and CPEs.Methods: Enterobacterales (n = 249), including natural AmpC producers, AHEs, CPEs and non-carbapenemase-producing carbapenem-resistant control strains were evaluated, using Carba NP, rCIM and rCIM-A. The rCIM-A differs from the rCIM by the addition of cloxacillin (400 μg/mL) to the initial antibiotic incubation step.Results: The rCIM-A yielded a sensitivity and specificity of 84.26% (95% CI: 76.00%-90.55%) and 99.29% (95% CI: 96.11%-99.98%), respectively, while those of the rCIM were 86.11% (95% CI: 78.13%-92.01%) and 80.85% (95% CI: 73.38%-86.99%), respectively; those of Carba NP were lower at 84.04% (95% CI: 75.05%-90.78%) and 91.37% (95% CI: 85.41%-95.46%), respectively, due to indeterminate results. The rCIM-A was capable of discriminating between AHEs and true CPEs, but still failed to identify OXA-23-producing Proteus mirabilis isolates and remained only partially reliable for identifying IMI-like producers and a few MBL (2 NDM-1, 1 LMB-1, 1 TMB-1 and 1 IMP-13) producers. One chromosomally encoded AmpC variant, MIR-10, gave repeatedly positive results using all three tests and was thus considered a false positive.Conclusions: Specificity for AHEs greatly improved with the rCIM-A without altering the test performance for the other resistance mechanisms. It may replace the rCIM as a cheap, easy, rapid and accurate CPE detection test. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
4. Evaluation of the rapid carbapenem inactivation method (rCIM): a phenotypic screening test for carbapenemase-producing Enterobacteriaceae.
- Author
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Muntean, Mădălina-Maria, Muntean, Andrei-Alexandru, Gauthier, Lauraine, Creton, Elodie, Cotellon, Garance, Popa, Mircea Ioan, Bonnin, Rémy A, Naas, Thierry, and Muntean, Madalina-Maria
- Subjects
AMIDASES ,DIAGNOSTIC errors ,DRUG resistance in microorganisms ,ENTEROBACTERIACEAE ,LONGITUDINAL method ,MICROBIAL sensitivity tests ,PHOTOMETRY ,POLYMERASE chain reaction ,PHENOTYPES ,GENETIC testing ,CARBAPENEMS ,SEQUENCE analysis - Abstract
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection.Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain. Growth of the indicator strain is monitored using a nephelometer. The performances of the rCIM were compared with the CIM and Carba NP tests using a collection of 113 well-characterized carbapenem-resistant enterobacterial isolates, including 85 carbapenemase producers and 28 non-carbapenemase producers. In addition, rCIM was compared with the Carba NP test and PCR sequencing in a prospective analysis of 101 carbapenem-resistant enterobacterial isolates addressed to the French National Reference Center for Antimicrobial Resistance in July 2017.Results and discussion: The rCIM correctly identified 84/85 carbapenemase producers and 28/28 non-carbapenemase producers, yielding a sensitivity of 99% and a specificity of 100%, slightly higher than the CIM and Carba NP test. In the prospective validation study, the rCIM showed a sensitivity and specificity of 97% and 95%, respectively. Two cephalosporinase-hyperproducing Enterobacter cloacae gave false-positive results, whereas an IMI-17-producing Enterobacter asburiae gave a false-negative result. The result was, however, positive when the isolate was grown on selective antibiotic-containing media.Conclusions: The rCIM is a rapid (less than 3 h), cheap and accurate test for the detection of CPEs, which can be implemented in low-resource settings, making it a useful tool for microbiology laboratories. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
5. Carbapenemase Inhibition Challenge Method: a novel way for evaluating the presence of carbapenemases - a pilot study.
- Author
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Muntean, Andrei-Alexandru, Poenaru, Adrian, Neagu, Andrei, Caracoti, Costin, Muntean, Mădălina-Maria, Zaharia, Dragoș Cosmin, Popa, Vlad Tudor, Miron, Alexandru Bogdan, and Popa, Mircea Ioan
- Subjects
CARBAPENEMS ,PUBLIC health ,KLEBSIELLA pneumoniae ,ESCHERICHIA coli ,MANN Whitney U Test - Abstract
Copyright of Infectio.ro is the property of MEDICHUB MEDIA, S.R.L. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
6. Carbapenemases in Enterobacteriaceae - overview and importance.
- Author
-
Muntean, Mădălina-Maria, Muntean, Andrei-Alexandru, and Popa, Mircea Ioan
- Abstract
Copyright of Infectio.ro is the property of MEDICHUB MEDIA, S.R.L. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
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