1. Chitin-Assisted Synthesis of CuS Composite Sponge for Bacterial Capture and Near-Infrared-Promoted Healing of Infected Diabetic Wounds.
- Author
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Luo B, Xiong Y, Cai J, Jiang R, Li Y, Xu C, and Wang X
- Subjects
- Animals, Mice, Wound Infection drug therapy, Wound Infection microbiology, Wound Infection pathology, Wound Infection therapy, Reactive Oxygen Species metabolism, Bandages, Staphylococcal Infections drug therapy, Staphylococcal Infections pathology, Wound Healing drug effects, Staphylococcus aureus drug effects, Copper chemistry, Copper pharmacology, Escherichia coli drug effects, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Chitin chemistry, Chitin pharmacology, Diabetes Mellitus, Experimental pathology, Infrared Rays
- Abstract
Diabetic wounds are prone to recurrent infections, often leading to delayed healing. To address this challenge, we developed a chitin-copper sulfide (CuS@CH) composite sponge, which combines bacterial trapping with near-infrared (NIR) activated phototherapy for treating infected diabetic wounds. CuS nanoparticles were synthesized and incorporated in situ within the sponge using a chitin assisted biomineralization strategy. The positively charged chitin surface effectively adhered bacteria, while NIR irradiation of CuS generated reactive oxygen species (ROS) heat and Cu
2+ to rapidly damage the trapped bacteria. This synergistic effect resulted in an exceptional antibacterial performance against E. coli (∼99.9%) and S. aureus (∼99.3%). The bactericidal mechanism involved NIR-induced glutathione oxidation, membrane lipid peroxidation, and increased membrane permeability. In diabetic mouse models, the CuS@CH sponge accelerated the wound healing of S. aureus infected wounds by facilitating collagen deposition and reducing inflammation. Furthermore, the sponge demonstrated good biocompatibility. This dual-functional platform integrating bacterial capture and NIR-triggered phototherapy shows promise as an antibacterial wound dressing to promote healing of infected diabetic wound.- Published
- 2024
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