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The main purpose of the study was to determine the suitable selection method for genetic improvement in chickpea. The current investigation was conducted at the research area of the Arid Zone Research Institute, Bhakkar, Punjab, Pakistan. Twelve chickpea genotypes were sown in RCBD following three replicates. Data regarding primary & secondary per plant, days for maturity, plant height as well as potential yield were recorded. The measured data were analyzed to principal component analysis (PCA), correlation, and path coefficient analysis. Analysis of variants revealed good variation among the accessions for the data recorded traits. Principal coefficient analysis differentiates all the traits into six PCs. Two components expressed more than one Eigenvalue which collectively contributed 80.12% towards the genetic variation. High positive loadings were expressed by the secondary branches per plant and pods per plant in all components. Path analysis revealed that secondary branches/plants expressed the highest direct positive effect (0.872) grain yield followed by the pods per plant (0.623). Analysis of correlation coefficient also confirmed the highest significant contribution of secondary branches per plant (91.57%) followed by pods (78.12%) and each plant's primary branches (64%). It was proved from the current experiment that Plants with secondary branches as well as pods may be focused on while assessing meaningful selection criteria for genetic improvement in chickpea. [ABSTRACT FROM AUTHOR]

This molecular epidemiological study was designed to determine the antimalarial drug resistance pattern, and the genetic diversity of malaria isolates collected from a war-altered Federally Administered Tribal Area (FATA), in Pakistan. Clinical isolates were collected from Bajaur, Mohmand, Khyber, Orakzai and Kurram agencies of FATA region between May 2017 and May 2018, and they underwent DNA extraction and amplification. The investigation of gene polymorphisms in drug resistance genes (dhfr, dhps, crt, and mdr1) of Plasmodium falciparum and Plasmodium vivax was carried out by pyrosequencing and Sanger sequencing, respectively. Out of 679 PCR-confirmed malaria samples, 523 (77%) were P. vivax, 121 (18%) P. falciparum, and 35 (5%) had mixed-species infections. All P. falciparum isolates had pfdhfr double mutants (C59R+S108N), while pfdhfr/pfdhps triple mutants (C59R+S108N+A437G) were detected in 11.5% of the samples. About 97.4% of P. falciparum isolates contained pfcrt K76T mutation, while pfmdr1 N86Y and Y184F mutations were present in 18.2% and 10.2% of the samples. P. vivax pvdhfr S58R mutation was present in 24.9% of isolates and the S117N mutation in 36.2%, while no mutation in the pvdhps gene was found. Pvmdr1 F1076L mutation was found in nearly all samples, as it was observed in 98.9% of isolates. No significant anti-folate and chloroquine resistance was observed in P. vivax; however, mutations associated with antifolate-resistance were found, and the chloroquine-resistant gene has been observed in 100% of P. falciparum isolates. Chloroquine and sulphadoxine-pyrimethamine resistance were found to be high in P. falciparum and low in P. vivax. Chloroquine could still be used for P. vivax infection but need to be tested in vivo, whereas a replacement of the artemisinin combination therapy for P. falciparum appears to be justified. [ABSTRACT FROM AUTHOR]

Climate change, water shortage, and costlier inputs are main issues in sugarcane productivity across sugarcane growing areas of the country. Sugarcane is a valuable cash crop and rewarding sector of agriculture. Varietal development process depends upon selection of successive clonal generations; among these preliminary yield trial (Nursery-III) is fourth selection stage. Thirty four (34) clones were tested in three sets using Randomized Complete Block Design (RCBD) with three replication during 2017-18 at research farm of Sugarcane Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan. The clones were evaluated using five basic biometric parameters keeping in view the farmer's and miller's demand. The genetic variability and performance of all clones was checked by using analysis of variance (ANOVA). Among all 34 clones, 16 clones were promoted into next selection stage while taking HSF-240 and SPF-245 as check varieties. The clones S2015-SL-10, S2015-SL-89, S2015-SL-101 and S2015-SL-624 produced sugar yield more than 13 tons per hectare. These clones may give future hope for current varietal development program subject to showing good performance in next selection stages because further selection stages will decide the fate of suchclones. The promising clones among these may be selected as candidate variety for approval after evaluating their performance in farmer's field trials and under national trials in different sugarcane growing areas of the country. [ABSTRACT FROM AUTHOR]