1. Early treated HIV-1 positive individuals demonstrate similar restriction factor expression profile as long-term non-progressors
- Author
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Sofie Rutsaert, Ward De Spiegelaere, Karen Vervisch, Linos Vandekerckhove, Sabine Kinloch-de Loes, Magdalena Sips, Wim Trypsteen, Clarissa Van Hecke, and Eva Malatinkova
- Subjects
Male ,0301 basic medicine ,Research paper ,Human immunodeficiency virus (HIV) ,HIV Infections ,Virus Replication ,medicine.disease_cause ,0302 clinical medicine ,Antiretroviral Therapy, Highly Active ,Early treatment ,HIV Seropositivity ,INFECTION ,Medicine and Health Sciences ,Medicine ,APOBEC3G ,Infectivity ,LTNP ,General Medicine ,Middle Aged ,Viral Load ,Phenotype ,PCR ,030220 oncology & carcinogenesis ,Host-Pathogen Interactions ,Disease Progression ,Female ,Adult ,INHIBITION ,HIV-1 infection ,General Biochemistry, Genetics and Molecular Biology ,Time-to-Treatment ,03 medical and health sciences ,Humans ,CONTROLLERS ,INTERFERON RESPONSE ,Seroconversion ,INTEGRASE ,business.industry ,Gene Expression Profiling ,DNA ,QUANTIFICATION ,CD4 Lymphocyte Count ,Chronic infection ,030104 developmental biology ,Gene Expression Regulation ,REPLICATION ,Immunology ,HIV-1 ,T-CELLS ,Tetherin ,Restriction factors ,business ,Biomarkers ,Cohorts ,SAMHD1 ,Restriction factor - Abstract
Background: A wide range of host restriction factors (RF) become upregulated upon HIV-1 infection to suppress viral infectivity and may aid viremic control in vivo. This cross-sectional study evaluated HIV-1 RFs and dependency factors in HIV infected individuals with progressive or non-progressive infection, as well as in early and late treated cohorts that exhibit different viro-immunological profiles due to differences in timing of treatment-initiation. Methods: The expression profile of IFIT1, MX1, APOBEC3G, SAMHD1, BST2 (encoding TETHERIN), TRIM5, MX2, SLFN11, PAF1, PSIP1 (encoding LEDGF/p75), and NLRX1 was measured by qPCR in 104 HIV-1 positive individuals: seroconverters (SRCV; n =19), long term non-progressors (LTNP; n =17), viremic progressors (VP; n =12), patients treated during seroconversion (Early treated; n =24) or chronic infection (Late treated; n =32), and non-infected controls. Findings: Expression levels of early treated HIV-1 positive individuals were significantly upregulated in comparison to late treated patients (IFIT1: p=0.0003; MX1: p=0.008; APOBEC3G: p=0.002; SAMHD1: p=0.0008; SLFN11: p
- Published
- 2019