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Abstract Hydrogen polysulfides (H 2 S n , n > 1) influence a variety of important biological functions and activities associated with hydrogen sulfide (H 2 S). The development of probes for rapid, selective, and sensitive detection of H 2 S n still remains a great challenge. Lysosome plays crucial roles in various physiological processes among living cells, which arouse high interest in detecting endogenous lysosome-targetable H 2 S n. To the best of our knowledge, there is no lysosome-targetable probe for H 2 S n has been reported. In this work, a new lysosome-targetable probe NIPY NF , based on the scaffold of imidazo [1,5-]pyridine, with a large Stokes shift (215 nm), low detection limit (84 nM), fast response time (6 min) and low cytotoxicity was designed and synthesized. Furthermore, NIPY NF was successfully applied into the cell imaging for detection of endogenous lysosome-targetable H 2 S n. Graphical abstract Image 1 Highlights • A novel lysosome-targetable probe NIPY NF was reported. • NIPY NF could discriminate H 2 S n over other analytes with high sensitivity, a low detection limit and a large Stokes shift. • NIPY NF could detect the endogenous H 2 S n in lysosome of living cells. [ABSTRACT FROM AUTHOR]

Hydrogen sulfide (H2S) is one of the endogenous regulators of many physiological processes. NIPY-OH based on an imidazo[1,5-α]pyridine has a large Stokes shift, high photostability with green-emission, relatively high fluorescence quantum yield. NIPY-OH could serve as an excellent candidate for the design of fluorescent probes. However, the reported probes cannot sufficiently take advantage of the optical properties of NIPY-OH due to the water-soluble problem. Herein, a new fluorescent probe NIPY-PBA, based on NIPY-OH, was designed for the detection of hydrogen sulfide. By rational design, compared with other probes, we successfully obtained the probe with higher sensitivity (limit of detection = 43 nM) and shorter response time (1.5 minutes) due to better water solubility. NIPY-PBA maintained the excellent optical properties of a 230-fold fluorescence enhancement with a large Stokes shift (208 nm) at the same time. Furthermore, NIPY-PBA was applied for the fluorescence imaging of H2S in living cells with low cytotoxicity, indicating NIPY-PBA has great potential to monitor H2S in biological systems. [ABSTRACT FROM AUTHOR]

Hydrogen sulfide (H2S), an endogenous gaseous signalling molecule, has attracted attention in biochemical research. The detection of H2S in living systems is essential for studying its functions. Although a variety of probes have been reported for the detection of cellular H2S, the development of rapid, highly selective and sensitive near-infrared (NIR) probes for in vivo detection of H2S still remains a challenging target. Herein we synthesized a near-infrared fluorescent probe Cy-PBA, with good selectivity, high sensitivity (limit of detection = 21 nM) and rapid response time (＜1.5 minutes). Importantly, Cy-PBA was successfully applied for fluorescence imaging of H2S in living cells, mice and tissues with low cytotoxicity, indicating that it owns broad application prospects in the detection of H2S in biological samples. [ABSTRACT FROM AUTHOR]