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Your search keyword '"Zhang, Deguo"' showing total 3 results
Start Over Author "Zhang, Deguo" Topic irisin Publication Year Range Last 10 years
3 results on '"Zhang, Deguo"'

1. Irisin functions to inhibit malignant growth of human pancreatic cancer cells via downregulation of the PI3K/AKT signaling pathway

Author

Zhang,Deguo, Zhang,Ping, Li,Luan, Tang,Nan, Huang,Fei, Kong,Xianguo, Tan,Xueying, and Shi,Guangjun

Subjects
pancreatic cancer, apoptosis, cell growth, migration, irisin, OncoTargets and Therapy, Original Research
Abstract

Deguo Zhang,1,2Ping Zhang,3Luan Li,2Nan Tang,1,2Fei Huang,2,4Xianguo Kong,5Xueying Tan,2,*Guangjun Shi,2,* 1Department of Hepatobiliary Surgery, The Affiliated Qingdao Chengyang District People’s Hospital of Qingdao University, Qingdao 266109, Shandong Province, People’s Republic of China; 2Department of Hepatobiliary Surgery, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao 266000, Shandong Province, People’s Republic of China; 3Department of Gynecology, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao 266000, Shandong Province, People’s Republic of China; 4Department of Gastrointestinal Hernia Surgery, Qianxinan People’s Hospital, Xingyi 562400, Guizhou Province, People’s Republic of China; 5Cardeza Foundation for Hematologic Research, Department of Medicine, Thomas Jefferson University, Philadelphia 19107, PA, USA*These authors contributed equally to this workCorrespondence: Xueying Tan; Guangjun ShiDepartment of Hepatobiliary Surgery, The Affiliated Qingdao Municipal Hospital of Qingdao University, No 1, Jiao Zhou Road, Shibei District, Qingdao 266000, Shandong Province, People’s Republic of ChinaTel +86 185 6269 2818;Tel +86 186 6167 5603Email sgjzp@hotmail.com;gdxbsys@163.comIntroduction: Irisin is a newly identified cytokine that has gained increasing attention because of its potential therapeutic applications in metabolic diseases and human cancers. Recently, accumulating evidence indicates that irisin plays an important role in the development and metastasis of various tumors. The aim of this study was to evaluate the effects and underlying mechanisms of irisin on malignant growth of pancreatic cancer cells.Materials and methods: The anti-proliferative effect of irisin was examined using the CCK-8 assay. Irisin-induced apoptosis was determined by the annexin V-FITC/PI staining assay. The effects of irisin on cell migration and invasion were assessed using the scratch-induced wound healing assay and transwell invasion assay, respectively. The expression and phosphorylation of signaling proteins were detected by Western blot analysis.Results: Our results showed that irisin inhibited cell proliferation and induced apoptosis of pancreatic cancer cells in a dose-dependent manner. In addition, irisin decreased the migration and invasion of pancreatic cancer cells. Finally, Western blot analysis revealed that irisin downregulated the PI3K/AKT signaling pathway.Conclusion: Our findings suggest that irisin is a novel therapeutic agent for pancreatic cancer.Keywords: irisin, pancreatic cancer, cell growth, migration, apoptosis

Published
2019

2. Review of Research on the Role of Irisin in Tumors.

Author

Zhang, Deguo, Tan, Xueying, Tang, Nan, Huang, Fei, Chen, Zengyin, and Shi, Guangjun

Subjects
*LITERATURE reviews, *WHITE adipose tissue, *ADIPOSE tissue diseases, *BROWN adipose tissue, *LUNG cancer, *TUMORS, *CANCER cell growth, *IRISIN
Abstract

Irisin is a newly discovered exercise-induced cytokine, produced by the proteolytic hydrolysis of fibronectin type III domain-containing protein 5 (FNDC5). Irisin is widely distributed in the human body and is involved in the browning of white adipose tissue, improving insulin resistance, improving cognitive function, and regulating bone metabolism. Recent studies have shown that irisin concentration is elevated in a variety of tumor tissues as compared with that in normal tissues. However, irisin has different effects on the proliferation and apoptosis of tumor cells in breast cancer, lung cancer, and liver cancer through various mechanisms. Irisin plays an important role in the occurrence, development, and metastasis of different tumors, suggesting that irisin can be used as a potential target for tumor diagnosis and treatment. Therefore, studying the expression and function of irisin in tumors may be of great significance for the prevention and treatment of tumors. This article reviews the research progress on the role of irisin in tumors. [ABSTRACT FROM AUTHOR]

Published
2020
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3. Irisin stimulates cell proliferation and invasion by targeting the PI3K/AKT pathway in human hepatocellular carcinoma.

Author

Shi, Guangjun, Tang, Nan, Qiu, Jiantao, Zhang, Deguo, Huang, Fei, Cheng, Yayu, Ding, Kun, Li, Weisheng, Zhang, Ping, and Tan, Xueying

Subjects
*POLYSACCHARIDE synthesis, *LIVER cancer, *DOXORUBICIN, *PHOSPHORYLATION, *CANCER chemotherapy
Abstract

Irisin is a newly identified myokine that may be cancer-associated, and its impact on liver cancer is unclear. To understand the roles of irisin in liver cancer, we investigated its effect in HepG2 and SMCC7721 hepatocellular carcinoma cells, and the underlying mechanisms. We determined irisin levels in liver tissues and serum samples obtained from patients by using real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Irisin levels in cancerous livers were significantly upregulated compared with those in control livers, but serum irisin levels remained unchanged. Additionally, we evaluated the effects of different concentrations of human recombinant modified and active (glycosylated) irisin (IM) or human recombinant nonmodified irisin (INM) on cell migration, proliferation, viability, and invasiveness. CCK8, transwell, and scratching assays demonstrated that irisin significantly increased cell proliferation, invasion, and migration through activation of the PI3K/AKT pathway. Irisin-induced cell proliferation, migration, and invasion were blocked by a PI3K inhibitor (LY294002). Irisin also decreased the cytotoxicity of doxorubicin in HepG2 cells. These data indicate that increased irisin levels may have protective roles in liver cancer cells through partial activation of the PI3K/AKT pathway, which may facilitate liver cancer progression and decrease the sensitivity to chemotherapy. [ABSTRACT FROM AUTHOR]

Published
2017
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