1. Lignolytic and hemicellulolytic enzyme cocktail production from Bacillus tequilensis LXM 55 and its application in pulp biobleaching.
- Author
-
Angural S, Kumar A, Kumar D, Warmoota R, Sondhi S, and Gupta N
- Subjects
- Biotechnology, Endo-1,4-beta Xylanases, Eucalyptus, Galactans metabolism, Hydrogen-Ion Concentration, Laccase biosynthesis, Mannans metabolism, Microscopy, Electron, Scanning, Plant Gums metabolism, RNA, Ribosomal, 16S metabolism, Temperature, Alkalies chemistry, Bacillus enzymology, Industrial Microbiology methods, Laccase chemistry, Paper
- Abstract
Bioprocessing of pulp requires lignolytic as well as hemicellulolytic enzymes. The present study is the first report of a cocktail of laccase (L), xylanase (X), and mannanase (M), from a single bacterium for pulp biobleaching. A novel strain Bacillus tequilensis LXM 55 produced thermo-alkali stable L + X + M. On optimization higher enzyme yield (IUml
-1 /fold increase) of laccase (396.35/24.16), xylanase (212.95/81.90) and mannanase (153.33/102.90) were achieved in the cocktail. Treatment of pulp with cocktail of enzymes led to 49.35% reduction in kappa number and considerable enhancement in the brightness (11.59%), whiteness (4.11%), and other pulp properties. Most importantly, no mediator system was required for the application of laccase. 40% less chlorine consumption was required to obtain the paper of the same quality as that of pulp treated without enzyme but with 100% chlorine. Therefore, this cocktail of enzymes is highly suitable for pulp biobleaching in the paper mill.- Published
- 2020
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