1. Indirect and competitive enzyme-linked immunosorbent assays for monitoring the humoral response against human VEGF.
- Author
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Sánchez Ramírez J, Morera Díaz Y, Musacchio Lasa A, Bequet-Romero M, Muñoz Pozo Y, Pérez Sánchez L, Hernández-Bernal F, Mendoza Fuentes O, Selman-Housein KH, Gavilondo Cowley JV, and Ayala Avila M
- Subjects
- Animals, Binding, Competitive, CHO Cells, Cricetulus, Goats, Humans, Immunoglobulin G blood, Immunoglobulin G immunology, Protein Binding, Vascular Endothelial Growth Factors blood, Cancer Vaccines immunology, Enzyme-Linked Immunosorbent Assay methods, Immunity, Humoral, Vascular Endothelial Growth Factor Receptor-2 metabolism, Vascular Endothelial Growth Factors immunology, Vascular Endothelial Growth Factors metabolism
- Abstract
CIGB-247, a VEGF-based vaccine, was studied in a clinical trial. This advance demands the refinement of the methodologies for assessment of vaccine immune responses. This study aimed to improve the performance of ELISAs for detecting IgG antibodies against human VEGF and the blocking activity of the serum to inhibit the VEGF/VEGFR2 interaction. The best experimental conditions were established through the evaluation of several blocking buffers, immobilization surfaces, and plate suppliers using human sera as test samples. As a result, two controlled ELISAs were used in testing of elicited immune response against VEGF in patients immunized with CIGB-247.
- Published
- 2016
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