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Simple Summary: The Przewalski's horse (Equus ferus przewalskii), the only extant species of wild horse, was extinct in the wild in 1960s. The wild horse has been successfully saved from extinction by captive breeding projects outside the historic range. Although multiple studies were conducted, the main problems such as loss of founder genes, inbreeding depression, hybridization with domestic horses, high morbidity and mortality, and a lack of reliable prevention strategies and treatment limitations of these problems are still unresolved and require further scientific effort. This review aims to increase understanding of the scientific attributes that make the survival of the species possible and how these attributes can be useful for appropriate design of conservation and management strategies oriented to improve the viability of the existing population of the species. This review summarizes studies on Przewalski's horse since its extinction in the wild in the 1960s, with a focus on the reintroduction projects in Mongolia and China, with current population status. Historical and present distribution, population trends, ecology and habitats, genetics, behaviors, conservation measures, actual and potential threats are also reviewed. Captive breeding and reintroduction projects have already been implemented, but many others are still under considerations. The review may help to understand the complexity of problem and show the directions for effective practice in the future. [ABSTRACT FROM AUTHOR]

Bluetongue virus (BTV), a member of the genus Orbivirus in the family Reoviridae, is transmitted by biting midges and causes severe disease in domestic and wild ruminants. In the present study, a BTV strain, BTV‐20/GX015/China/2013 (GX015), was isolated from sentinel cattle in Guangxi, China. Virus neutralization tests and phylogenetic analyses based on genomic segments 2 (S2) and 6 (S6) indicated that GX015 belongs to BTV serotype 20 (BTV‐20) and represents a new topotype within BTV‐20 strains, which makes GX015 the first BTV‐20 strain isolated in China. Genomic analyses suggested that the 10 genomic segments of GX015 originated from a reassortment event, in which S2 and S6 are derived from exotic BTV‐20 strains (South Africa or Australia), whereas the remaining eight genomic segments are apparently of Chinese origin and most likely share the same ancestor with a Taiwanese BTV‐12 strain. Importantly, we evaluated the infectivity and pathogenicity of the BTV‐20 strain in mice lacking the interferon receptor (IFNAR–/– mice, a good animal model for studying the pathogenesis, virulence and transmission of BTVs) and sheep for the first time, and found that GX015 causes severe disease and death in IFNAR–/– mice and clinical signs and viraemia in the natural host sheep. These results improve our understanding of the genetic characteristics, diversity and pathogenicity of BTVs, which is important for developing diagnostic methods and vaccines for the surveillance and prevention of bluetongue disease. [ABSTRACT FROM AUTHOR]

Background: This study was aimed to investigate whether patients with epilepsy (PWE) have higher depression and anxiety levels than the normal population in low-risk areas for coronavirus disease 2019 (COVID-19) in the northern part of Guizhou Province, China, during the COVID-19 epidemic, to evaluate their knowledge on COVID-19, and to analyze related factors for the psychological distress of PWE at this special time. Methods: The survey was conducted online from February 28, 2020 to March 7, 2020 via a questionnaire. PWE from the outpatient clinic of epilepsy of the Affiliated Hospital of Zunyi Medical University, and healthy people matched for age and sex, participated in this study. Mental health was assessed via a generalized anxiety self-rating scale (GAD-7) and the self-rating depression scale (PHQ-9). The knowledge of COVID-19 in both groups was investigated. Results: There were no significant differences in the general demographics between the PWE and healthy control groups. The scores of PHQ-9 (P < 0.01) and GAD-7 (P < 0.001) were higher in the PWE group than in the healthy group. There was a significant difference in the proportions of respondents with different severities of depression and anxiety, between the two groups, which revealed significantly higher degree of depression and anxiety in PWE than in healthy people (P = 0, P = 0). Overwhelming awareness and stressful concerns for the pandemic and female patients with epilepsy were key factors that affect the level of anxiety and depression in PWE. Further, the PWE had less accurate knowledge of COVID-19 than healthy people (P < 0.001). There was no statistically significant difference between the two groups in the knowledge of virus transmission route, incubation period, susceptible population, transmission speed, clinical characteristics, and isolation measures on COVID-19 (P > 0.05). PWE knew less about some of the prevention and control measures of COVID-19 than healthy people. Conclusions: During the COVID-19 epidemic, excessive attention to the epidemic and the female sex are factors associated with anxiety and depression in PWE, even in low-risk areas. [ABSTRACT FROM AUTHOR]

The hog deer Axis porcinus formerly occurred in south-west China but has not been recorded there since 1965. To investigate the current status of the species in China, we conducted interviews, and transect and camera-trap surveys during October 2018–June 2020 to search for signs of hog deer across its historical range in the country. We interviewed 50 local inhabitants and surveyed 14 line transects in four counties of Lincang City, Yunnan Province. The camera traps were deployed in Nangunhe Nature Reserve (39.4 km of transects, 82 camera stations, 15,120 camera days) and Daxueshan Nature Reserve (41.1 km of transects, 68 camera stations, 13,554 camera days). We found no hog deer tracks and no hog deer were trapped by cameras. The floodplain grasslands preferred by hog deer along Nanting River have been transformed into agriculture plantations and human settlements. Our findings suggest that hog deer may have been extirpated from China, most likely as a result of habitat loss and overhunting. The conservation priorities for this species in China are the establishment of a protected area in the Nanting River watershed, restoration of habitat and reintroduction of individuals from range countries. [ABSTRACT FROM AUTHOR]

Many ungulates are reported to use natural or artificial licks with seasonal patterns around the world. From December 2016 to August 2017, we used infrared camera to record the use of artificial licks in wild sika deer (Cervus nippon) in Zhejiang Qingliangfeng National Nature Reserve, China. We explored the daily rhythm, seasonal pattern and sex difference in lick utilization. In total, 12,043 videos and 22,901 pictures were collected. Our results showed that: (1) the lick visiting frequency was higher at night than that during daytime; (2) the difference in lick visiting frequency between females and males disappeared after taking into account of sex ratio; (3) the lick duration peaked in April during a year. These findings suggested that there were clear daily rhythm and seasonal pattern of lick use in sika deer. Seasonal change in lick use intensity was consistent with our prediction. These variations in lick use might be driven by both the physiological needs of the mineral elements in different life stages and the seasonal changes in climate and food. The reserve management authority should pay more attention to the supplement of licks in spring and summer to fulfill animal's physiological needs. [ABSTRACT FROM AUTHOR]

Wild sika deer ( Cervus nippon ) are threatened by habitat loss and fragmentation throughout East China. Using 18-year demographic data, we conducted a viability analysis to predict the fate of a wild sika deer population threatened by loss of suitable habitat in the Taohongling National Nature Reserve, Southeast China. We simulated performance of proposed management interventions — population recruitment and habitat restoration — in preventing the sika deer population from declining. In the scenario where 60% or 30% of suitable habitat was lost, the population viability model predicted 99.6% and 85.5% probability of population decline by 40 years, respectively. The modelling demonstrated that carrying capacity, which was affected by vegetation succession and socioeconomic development, would restrict the growth of the sika deer population. The survival rate of adults is the element most affecting population growth, yet supplementation of adult deer was predicted to be less effective than habitat restoration in reducing risk of population decline in all habitat scenarios. Large-area but less-intensive restoration should be considered at initial stage of the intervention if the funding was sufficient to support. Regarding these findings, we recommend future management interventions to target towards increasing suitable habitat as well as preventing habitat loss and fragmentation in Taohongling National Nature Reserve. The zoning scheme and its management need to be regulated with conservation incentives. [ABSTRACT FROM AUTHOR]

Despite the increasing contribution of the automotive industry to China's national economy, CO2 emissions have become a challenge. However, the research about its energy consumption and carbon emissions is lacking. The significance of this study is to fill the research gap and provide suggestions for China's automotive industry to reduce its carbon emissions. In this paper, the extended logarithmic Division index (LMDI) method is adopted to decompose the factors affecting carbon emissions and determine the key driving forces. According to provincial statistical data in China in 2017, the annual emissions of six provinces exceeded five million tons, accounting for 55.44% of the total emissions in China. The largest source of emissions in China is in Jilin Province, followed by Jiangsu, Shandong, Shanghai, Hubei and Henan. The decomposition results show that investment intensity effect is the greatest factor for CO2 emissions, while R&D intensity and energy intensity are the two principal factors for emission reduction. After the identification of driving factors, mitigation measures are proposed considering the current state of affairs and real situation, including improving energy structure, accelerating product structure transformation, stimulating sound R&D investment activities, promoting energy conservation and new energy automobile industry development and boosting industrial cluster development. [ABSTRACT FROM AUTHOR]

Background: Indoor environmental quality significantly influences the occurrence of asthma attack. Zunyi District has abundant coal reserves and is regarded as one of the cities that are most severely polluted by high levels of particulate matter in China. This study aimed to examine the correlation of indoor exposure with adult respiratory health, as well as the differences in effect between winter and summer.Methods: A cross-sectional epidemiological study was conducted among 1207 adult residents in Zunyi, Guizhou Province of Southwest China in winter and summer. Data on health variables related to asthma and home environmental factors were collected using a modified European Community Respiratory Health Survey II questionnaire. The following data were obtained: samples of particulate matter 2.5 (PM2.5) inside and outside the households under study (n = 20); lung function status, including peak expiratory flow rate, forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), and FEV1/FVC ratio.Result: The odds ratio (OR) for asthma-like symptoms and asthma in adults using coal stove for cooking or warming, relative to non-users, was 1.73 (95% CI, 1.11-2.69) in winter vs. 1.30 (95% CI, 0.79-2.14) in summer. Adult residents with exposure to cooking oil fumes were at a considerably higher risk of asthma-like symptoms and asthma [OR = 2.65 (95% CI, 1.25 to 5.61) in winter vs. OR = 7.93 (95% CI, 2.54 to 24.75] in summer] than those without such exposure. The prevalence of asthma-like symptoms and asthma was significantly higher in adults with high kitchen risk scores or high sleeping-area risk scores than in those with low scores in both seasons (p < 0.05). The relative kitchen and sleeping area PM2.5 concentrations were higher in winter than in summer (p < 0.05). Lung function was negatively associated with indoor kitchen and sleeping area relative PM2.5 concentration in winter rather than summer (p < 0.001). The effect of exposure to indoor risk factors on lung function among the residents was greater in winter than in summer (p < 0.001).Conclusion: Exposure to indoor risk factors, such as aerocontaminants from coal combustion, causes asthma symptoms and reduces pulmonary function. The effect of indoor risk factors on respiratory health among adults with such exposure was greater in winter than in summer. [ABSTRACT FROM AUTHOR]

The article discusses initiatives taken by China to deal with illegal ivory trade that has become a threat to wildlife. Topics discussed include the ban on import of ivory carvings announced by the State Forestry Administration of China, the Demand-side Strategies for Curbing Illegal Ivory Trade workshop that was organized to understand economy of illegal trade and participation in the law enforcement operation Operation Cobra III that led to several seizures such as elephant ivory.

The production and sales of electric vehicles in China are continuously increasing, and a massive wave of scrap batteries is about to emerge. However, there are many challenges in the power battery recycling, such as a disorganized recycling system, a lack of standards, and low efficiency. In addition, the power battery recycling supply chain faces issues such as a lack of transparency, inadequate data management, low trustworthiness, and counterfeit products. Therefore, it's crucial to establish an efficient and sustainable power battery supply chain recycling system. Blockchain technology can provide solutions in decentralized data management, privacy protection, and anti-counterfeiting traceability. It addresses existing problems and improves the Power Batteries Reverse Supply Chain (PBRSC) ecosystem. Thus, this paper proposes a blockchain-based PBRSC management integration framework. Firstly, a four-layer blockchain power battery recycling platform is designed. Secondly, based on the proposed PBRSC framework, this paper studies three key component technologies: double-chain structure and its storage mechanism, information tracing module, and smart contract enabled battery trading management. Then, the validity and feasibility of the double-chain structure was demonstrated experimentally. Finally, the above architecture and key technologies are implemented in the system, the main functional modules are displayed on the interface, and the effectiveness of the framework is explained in cooperation with related enterprises. [ABSTRACT FROM AUTHOR]

While the concept of remanufacturing is gaining popularity in practice, its effect on environmental protection and energy saving is significant, which has attracted widespread concerns, especially in the automotive components field. Automotive components remanufacturing (ACR), as a prominent part in automotive life-cycle development, can dramatically reduce resources-waste and mitigate energy-crisis, which has become a prominent direction to promote sustainable development of Chinese economy. Thus, influence factors analysis and enterprise operation patterns assessment which primarily contribute to ACR industry development are of paramount importance. This paper elaborates the criteria influencing the ACR industry operation and analyzes the main operation patterns for ACR in China. To overcome the uncertainty of the evaluation process, a new hybrid multi-criterion decision-making (MCDM) model that combines fuzzy AHP (analytical hierarchy process) and fuzzy G-TOPSIS (combining gray relation analysis and technique for order performance by similarity to ideal solution) is proposed to determine weights of influence criteria and evaluate ACR production operation patterns. In addition, sensitivity analysis is applied to monitor the robustness of the method. The study refers to the experience of Chinese end-of-life vehicle (ELV) laws and implementation requirements of extended producer responsibility (EPR). The obtained results provide the new guideline to revise the regulations of Chinese ACR industry. [ABSTRACT FROM AUTHOR]

Wastewater pollution has been considered as a prominent bottleneck restricting global sustainable development. China is one of the largest discharges and eco-environmental damages of wastewater in the world. Through analyzing wastewater discharge data using emergy method in China from 2011 to 2017, the wastewater eco-environmental damage of 31 provinces is calculated with GDP and area to reveal the fundamental origins of inflection point of wastewater discharge in China. Studies results show that, (i) Chinese "12th Five-Year Plan" (2011-2015) is a watershed in wastewater discharge, and the eco-environment damages caused by China's wastewater accounted for more than 1/4 of GDP; (ii) China has the great potential to reduce eco-environment damages of 1.73 trillion $/year; (iii) In 2016 and 2017, wastewater eco-environmental damage has decreased by about 50% compared with that in 2015, and the effect of government policies was remarkable. We conclude that decoupling of China's economic development form eco-environmental damages of wastewater is began to appear, the strict formulation and implementation of China's environmental policies and the green upgrading of industrial structure are main driving forces, and it is little correlation with economic slowdown. This study offers the detailed list of China wastewater pollution and reveals the relationship between wastewater eco-environmental damages and economic development, and shows the experience and achievements of the Chinese government in the treatment of wastewater pollution, which provides a useful reference for the treatment of wastewater pollution in the world., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021. Published by Elsevier B.V.)

Kashmir musk deer Moschus cupreus (KMD) are the least studied species of musk deer. We compiled genetically validated occurrence records of KMD to construct species distribution models using Maximum Entropy. We show that the distribution of KMD is limited between central Nepal on the east and north-east Afghanistan on the west and is primarily determined by precipitation of driest quarter, annual mean temperature, water vapor, and precipitation during the coldest quarter. Precipitation being the most influential determinant of distribution suggests the importance of pre-monsoon moisture for growth of the dominant vegetation, Himalayan birch Betula utilis and Himalayan fir Abies spectabilis, in KMD's preferred forests. All four Representative Concentration Pathway Scenarios result an expansion of suitable habitat in Uttarakhand, India, west Nepal and their associated areas in China in 2050s and 2070s but a dramatic loss of suitable habitat elsewhere (Kashmir region and Pakistan-Afghanistan border). About 1/4 th of the current habitat will remain as climate refugia in future. Since the existing network of protected areas will only include a tiny fraction (4%) of the climatic refugia of KMD, the fate of the species will be determined by the interplay of more urgent short-term forces of poaching and habitat degradation and long-term forces of climate change.

Using the Yellow-River-Source National Park (YRSNP) as a study site, an unmanned aerial vehicle (UAV) remote sensing and line transect method was used to investigate the number of wild herbivorous animals and livestock, including the kiang ( Equus kiang ) and Tibetan gazelle ( Procapra picticaudata ). A downscaling algorithm was used to generate the forage yield data in YRSNP based on a 30-m spatial resolution. On this basis, we estimated the forage-livestock balance, which included both wild animals and livestock, and analyzed the effects of functional zone planning in national parks on the forage-livestock balance in YRSNP. The results showed that the estimates of large herbivore population numbers in YRSNP based on population density in the aerial sample strips, which were compared and validated with official statistics and warm season survey results, indicated that the numbers of kiangs and Tibetan gazelles in the 2017 cold season were 12,900 and 12,100, respectively. The numbers of domestic yaks, Tibetan sheep, and horses were 53,400, 76,800, and 800, respectively, and the total number of sheep units was 353,200. The ratio of large wild herbivores and livestock sheep units was 1:5. Large wild herbivores have different preferences for functional zones, preferring ecological restoration areas consisting mainly of sparse grassland. The grazing pressure indices of the core reserve areas and ecological restoration areas were 0.168 and 0.276, respectively, indicating that these two regions still have high grazing potential. However, the grazing pressure index of the traditional utilization areas was 1.754, indicating that these grasslands are severely overloaded. After the planning and implementation of functional zones, the grazing pressure index of YRSNP was 1.967. Under this measure, the number of livestock was not reduced and the grazing pressure nearly doubled, indicating that forage-livestock conflict has become more severe in YRSNP.

Background: Tetralogy of Fallot (TOF) accounts for ∼10% of congenital heart disease cases. The blood vessel epicardial substance ( BVES ) gene has been reported to play a role in the function of adult hearts. However, whether allelic variants in BVES contribute to the risk of TOF and its possible mechanism remains unknown. Methods: The open reading frame of the BVES gene was sequenced using samples from 146 TOF patients and 100 unrelated healthy controls. qRT-PCR and western blot assays were used to confirm the expression of mutated BVES variants in the TOF samples. The online software Polyphen2 and SIFT were used to predict the deleterious effects of the observed allelic variants. The effects of these allelic variants on the transcriptional activities of genes were examined using dual-fluorescence reporter assays. Results: We genotyped four single nucleotide polymorphisms (SNPs) in the BVES gene from each of the 146 TOF patients. Among them, the minor allelic frequencies of c.385C>T (p.R129W) were 0.035% in TOF, but ∼0.025% in 100 controls and the Chinese Millionome Database. This allelic variant was predicted to be a potentially harmful alteration by the Polyphen2 and SIFT softwares. qRT-PCR and western blot analyses indicated that the expression of BVES in the six right ventricular outflow tract samples with the c.385C>T allelic variant was significantly downregulated. A dual-fluorescence reporter system showed that the c.385C>T allelic variant significantly decreased the transcriptional activity of the BVES gene and also decreased transcription from the GATA4 and NKX2.5 promoters. Conclusions: c.385C>T (p.R129W) is a functional SNP of the BVES gene that reduces the transcriptional activity of BVES in vitro and in vivo in TOF tissues. This subsequently affects the transcriptional activities of GATA4 and NKX2.5 related to TOF. These findings suggest that c.385C>T may be associated with the risk of TOF in the Han Chinese population.

We analyzed Chinese Miao population samples (n = 268) from Guizhou province, Southwest China, with 19 X-chromosomal short tandem repeats (STRs) included in the AGCU X19 amplification kit. The combined PE is 0.999999922 and the combined PDs in males and females are 0.9999999999999999999994 and 0.9999999999998, respectively. The mean paternity exclusion change values are larger than 0.99999996. The HDs in seven linkage groups vary from 0.9324 to 0.9968. Subsequently, comparison among different Chinese populations shows that the substructures of Chinese were significantly influenced by ethno-linguistic rather than geographical boundaries, including most prominently Turkic-speaking and Tibeto-Burman-speaking populations.

The gastrointestinal tract of primates harbors a complex microbial community, playing an essential role in the degradation of otherwise indigestible structural carbohydrates. The phylogenetic and functional diversity of the bacterial community in the feces as a surrogate for the gastrointestinal tract of wild Sichuan snub-nosed monkeys (Rhinopithecus roxellana, N = 6) was characterized based on sequence analysis of 16S rRNA genes. A sex comparison was conducted, with a prior hypothesis that the abundances of the bacterial taxa and/or functional categories associated with energy and nutrient metabolism would be higher in adult females (N = 3) due to the higher reproductive costs compared to adult males (N = 3). Ten phyla were identified in all samples, among which Bacteroidetes and Firmicutes were the predominant. Included in the above two phyla, the members of Prevotellaceae (Prevotella in particular) and Ruminococcaceae were highly abundant, which are common bacteria in the gastrointestinal tract of primates and can degrade various structural carbohydrates such as cellulose, hemicellulose, and pectin. This functionality was in line with the high abundances of the metagenomes associated with carbohydrate metabolism. Consistent with our hypothesis, the abundances of the metagenomes associated with energy metabolism, folding/sorting and degradation, glycan biosynthesis and metabolism, and metabolism of amino acids were higher in adult females relative to adult males. Sex differences were also detected in the bacterial community structure, although no sex differences in the proportions of any bacterial taxa were found likely due to the small sample size. These results suggested that gastrointestinal bacterial communities may aid adult females in increasing energy and nutrition utilization efficiencies compared to adult males. Fecal bacterial communities were found to be more similar between individuals in adult females than in adult males. Our study presented the first examination of the fecal bacterial diversity of a little-studied, endangered foregut fermenter., (© 2018 Wiley Periodicals, Inc.)

The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) has been counted on for conserving threatened marine fish since it regulates the commercial international trade of these species. Implementation of the international treaty for Mantas included on CITES Appendix II is challenging due to insufficient information on species identification and markets management. To fill the gap in such aspects, we identified five species of Mobulid rays (Mobula spps. and Manta spp) by using COI and NADH2 mtDNA markers in dried ray gill rakers from Chinese markets, namely, Mobula japonica (representing 54.8% of the sample set), M. tarapacana (14.4%), M. kuhlii (13.3%), M. thurstoni (6.4%), along with Manta birostris (11.2%; CITES Appendix II). The utilization and conservation statuses of these species were discussed. Based on combination of DNA barcodes and key morphological characters, we developed a three-step process for identifying the gill rakers of Mobulid rays which has been adopted by frontline enforcement in China. We hope that our work can serve as a foundation and basis to reinforce objectives of international treaties, regulation of consumer-driven markets, regional cooperation, and national fishery management on endangered elasmobranchs in China as well as related countries.