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Natural rubber is an important national strategic and industrial raw material. The leaf anthracnose of rubber trees caused by the Colletotrichum species is one of the important factors restricting the yields of natural rubber. In this study, we isolated and identified strain Bacillus velezensis SF334, which exhibited significant antagonistic activity against both C. australisinense and C. siamense, the dominant species of Colletotrichum causing rubber tree leaf anthracnose in the Hainan province of China, from a pool of 223 bacterial strains. The cell suspensions of SF334 had a significant prevention effect for the leaf anthracnose of rubber trees, with an efficacy of 79.67% against C. siamense and 71.8% against C. australisinense. We demonstrated that SF334 can lead to the lysis of C. australisinense and C. siamense mycelia by causing mycelial expansion, resulting in mycelial rupture and subsequent death. B. velezensis SF334 also harbors some plant probiotic traits, such as secreting siderophore, protease, cellulase, pectinase, and the auxin of indole-3-acetic acid (IAA), and it has broad-spectrum antifungal activity against some important plant pathogenic fungi. The genome combined with comparative genomic analyses indicated that SF334 possesses most genes of the central metabolic and gene clusters of secondary metabolites in B. velezensis strains. To our knowledge, this is the first time a Bacillus velezensis strain has been reported as a promising biocontrol agent against the leaf anthracnose of rubber trees caused by C. siamense and C. australisinense. The results suggest that B. velezensis could be a potential candidate agent for the leaf anthracnose of rubber trees. [ABSTRACT FROM AUTHOR]

Background: Robinia pseudoacacia is a widely planted pioneer tree species in reforestations on barren mountains in northern China. Because of its nitrogen-fixing ability, it can play a positive role in soil and forest restoration. After clear-cutting of planted stands, R. pseudoacacia stands become coppice plantations. The impacts of shifting from seedling to coppice stands on soil bacterial community and soil properties have not been well described. This study aims to quantify how soil properties and bacterial community composition vary between planted seedling versus coppice stands. Methods: Nine 20 m × 20 m plots were randomly selected in seedling and coppice stands. The bulk soil and rhizosphere soil were sampled in summer 2017. Bulk soil was sampled at 10 cm from the soil surface using a soil auger. Rhizosphere soil samples were collected using a brush. The soil samples were transported to the laboratory for chemical analysis, and bacterial community composition and diversity was obtained through DNA extraction, 16S rRNA gene amplification and high-throughput sequencing. Results: The results showed that, compared to seedling plantations, soil quality decreased significantly in coppice stands, but without affecting soil exchangeable Mg2+ and K+. Total carbon (C) and nitrogen (N) were lower in the rhizosphere than in bulk soil, whereas nutrient availability showed an opposite trend. The conversion from seedling to coppice plantations was also related to significant differences in soil bacterial community structure and to the reduction of soil bacterial α-diversity. Principal component analysis (PCA) showed that bacterial community composition was similar in both bulk and rhizosphere soils in second-generation coppice plantations. Specially, the conversion from seedling to coppice stands increased the relative abundance of Proteobacteria and Rhizobium, but reduced that of Actinobacteria, which may result in a decline of soil nutrient availability. Mantel tests revealed that C, N, soil organic matter (SOM), nitrate nitrogen (NO3−-N) and available phosphorus positively correlated with bacterial community composition, while a variation partition analysis (VPA) showed that NO3−-N explained a relatively greater proportion of bacterial distribution (15.12%), compared with C and SOM. Surprisingly, N showed no relationship with bacterial community composition, which may be related to nitrogen transportation. Conclusions: The conversion from seedling to coppice stands reduced soil quality and led to spatial-temporal homogenization of the soil bacterial community structure in both the rhizosphere and bulk soils. Such imbalance in microbial structure can accelerate the decline of R. pseudoacacia. This may affect the role of R. pseudoacacia coppice stands in soil and forest restoration of barren lands in mountain areas. [ABSTRACT FROM AUTHOR]

• R. pseudoacacia increased the fine particles and reduced coarse particles content. • R. pseudoacacia significantly reduced D 0 , D 1 , D 2 but increased Δα and Δf. • R. pseudoacacia suppress the coarsening and deterioration of the surface. The soil particle size distribution (PSD) is a fundamental physical property that can affect soil nutrients, soil structure characterization and soil hydraulic properties. However, the effect of plant expansion on soil PSD is not clear. Therefore, in this study, fractal theory was applied to quantitatively describe the PSD. On Mount Tai, artificial afforestation with Robinia pseudoacacia is helpful in restoring ecosystems and improving soil quality. However, clonal spread allows R. pseudoacacia to easily escape cultivation, leading to the formation of mixed forests. Therefore, exploring the effect of R. pseudoacacia expansion on soil PSD is important. The results show that 1) R. pseudoacacia expansion increased the clay and silt contents and reduced the sand content; 2) R. pseudoacacia expansion significantly reduced the capacity dimension, entropy dimension, and correlation dimension and increased the width and asymmetry of the singularity spectra but was unrelated to the entropy dimension/capacity dimension; and 3) the capacity dimension was significantly positively correlated with the sand content and significantly negatively correlated with the silt and clay contents, but the asymmetry of the soil PSD was inconsistent with the capacity dimension. In summary, R. pseudoacacia can reduce the heterogeneity of the PSD by increasing the distribution range and density of the PSD. Our results provide important insight into the effect of plant expansion on soil PSD. [ABSTRACT FROM AUTHOR]

Background: The psychological status of Chinese postgraduate students majoring in stomatology after the COVID-19 restrictions still remains unclear. The objective of this study is to evaluate the mental status through a cross-sectional survey and gather related theoretical evidence for psychological intervention on postgraduate students majoring in stomatology., Methods: An online survey was administered, and subjective well-being, anxiety, stress and depression symptoms were assessed using the 5-item World Health Organization Well-Being Index (WHO-5), item Generalized Anxiety Disorder Scale (GAD-7), 10-item Perceived Stress Scale (PSS-10), and Patient Health Questionnaire-9 (PHQ-9), respectively, wherein suicidal ideation and sleep-related problems were measured with PHQ-9 and Insomnia Severity Index (ISI)., Results: A total of 208 participants who completed one questionnaire were considered as valid. It was found that female respondents generally exhibited significantly higher levels of PSS-10, PHQ-9, and GAD-7 scores and shorter physical activity hours than male students. Students from rural areas demonstrated significantly higher levels of PHQ-9, suicidal ideation, and less portion of good or fair family economic support. Additionally, individuals from only-child families reported increased levels of activity hours (1.78 ± 2.07, p  = 0.045) and a higher portion (55.10%, p  = 0.007) of having clear future plan as compared with multiple-child families. The risk factors for anxiety symptoms (GAD-7 score) were higher scores of PSS-10 ( OR  = 1.15, 95% CI = 1.09-1.22), PHQ-9 ( OR  = 1.35, 95% CI = 1.22-1.49), and ISI-7 ( OR  = 1.14, 95% CI = 1.06-1.23), while owning a clear graduation plan was the protective factor ( OR  = 0.55, 95% CI = 0.31-0.98). Moreover, the risk factors for depressive symptoms (PHQ-9) included PSS-10 ( OR  = 1.10, 95% CI = 1.04-1.16), GAD-7 ( OR  = 1.38, 95% CI = 1.25-1.52), suicidal ideation ( OR  = 5.66, 95% CI = 3.37-9.51), and ISI-7 ( OR  = 1.17, 95% CI = 1.09-1.25). Approximately 98.08% of Chinese postgraduates studying stomatology reported experiencing at least moderate stress after the COVID-19 restrictions., Conclusion: Within the limitations of this study, senior students were more inclined to stress, while anxiety symptoms were related to severer levels of stress, depression, and insomnia. Depressive symptoms were associated with higher levels of stress, anxiety, insomnia, suicidal ideation, and lower levels of self-reported well-being. Thus, psychological interventions for postgraduates should be timely and appropriately implemented by strengthening well-being, reasonably planning for the future, and good physique, thereby mitigating the psychological issues after COVID-19 restrictions., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhang, Jia, MuLaTiHaJi, Mi, Mei, Sun, Shi, Zhang, Zhang, Zou, Niu and Dong.)

To study the fractal dimensions of the soil particle size distributions (PSDs) within different plantations (of Pinus densiflora, Quercus acutissima, Robinia pseudoacacia, and Larix kaempferi) and evaluate PSDs as an indicator of the likelihood of Robinia pseudoacacia invasion, the soil porosity of 0-20 cm soil layers was measured at different plantations in the Yaoxiang Forest Farm, Shandong Province, China. The results showed that the fractal dimension (D m ) values varied from 2.59 to 2.70 among the different plantations and were significantly negatively correlated to sand content and positively correlated to silt content and clay content. Significant negative correlations were observed between D m and both soil organic matter (SOM) (P < 0.05) and available phosphorus (P < 0.01). The multifractal entropy dimension (D 1 ) and entropy dimension/capacity dimension (D 1 /D 0 ) parameters were not significantly correlated with SOM, although significant correlations were found between SOM and each of D 0 , Δα, and Δf(α). Compared with the other plantations, the Robinia pseudoacacia plantation had higher nutrient contents, higher D 0 and D 1 values and lower D m values. Based on principal component analysis (PCA) ordination, we concluded that Robinia pseudoacacia and Pinus densiflora shared a similar habitat and that Robinia pseudoacacia is more likely to invade Pinus densiflora plantations for soil.