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Background: Hookworm disease is endemic in China and is widespread globally. The disease burden to humans is great. Methods: The study described the national surveillance of hookworm implemented in 31 provinces/autonomous regions/municipalities (P/A/Ms) of China in 2019. Each P/A/M determined the number and location of surveillance spots (counties). A unified sampling method was employed, and at least 1000 subjects were investigated in each surveillance spot. The modified Kato-Katz thick smear method was employed for stool examination. Fifty samples positive with hookworm eggs were cultured in each surveillance spot to discriminate species between A. duodenale and N. americanus. Twenty-five soil samples were collected from each surveillance spot and examined for hookworm larva. The 2019 surveillance results were analyzed and compared with that of 2016–2018. Results: A total of 424766 subjects were investigated in 31 P/A/Ms of China in 2019, and the overall hookworm infection rate was 0.85% (3580/424766). The weighted infection and standard infection rates were 0.66% (4288357/648063870) and 0.67% (4343844/648063870), respectively. Sichuan province had the highest standard infection rate (4.75%) in 2019, followed by Chongqing (2.54%) and Hainan (2.44%). The standard infection rates of other P/A/Ms were all below 1%, with no hookworm detected in 15 P/A/Ms. The standard hookworm infection rate in the males and the females were 0.61% (2021216/330728900) and 0.71% (2267141/317334970), respectively, with a significant difference between different genders (χ2 = 17.23, P<0.0001). The highest standard hookworm infection rate (1.97%) was among age ≥ 60 years, followed by 45~59 years (0.77%), 15~44 years (0.37%), and 7~14 years (0.20%). The lowest standard infection rate was among the 0~6 years age group (0.12%). A significant difference was observed among different age groups (χ2 = 2 305.17, P<0.0001). The constitute ratio for N. americanus, A. duodenale, and coinfection was 78.70% (1341/1704), 2.03% (346/1704), and 1.00% (17/1704), respectively. The detection rate of hookworm larva from soil was 3.45% (71/2056). Conclusion: The national surveillance showed that the hookworm infection rate has been decreasing annually from 2016 to 2019, and it is now below 1%. China has made significant progress in controlling hookworm. The national surveillance system is an important way to understand the endemic status and provide important information in this process and thus needs to be continually optimized. Author summary: Hookworm disease is endemic in China. The national surveillance system on important parasitic diseases including hookworm infection has been established in China. Stool samples were collected from participants, and the Kato-Katz method was applied to detect helminth eggs while samples with hookworm eggs were further cultured to differentiate the species of the parasite. Additionally, soil samples were collected and examined for hookworm larva. In 2019, the overall infection rate of hookworm in China was 0.85% (3580/424766). High prevalence was demonstrated in Western and Southern China, including Sichuan (4.75%), Chongqing (2.54%) and Hainan (2.44%). The prevalence was high in the females (0.71%) than in the males (0.61%), while it was high in older population especially those age over 60 years. N. americanus dominated the hookworm species. The prevalence of hookworm in soil was 3.45%. Overally, hookworm infection decreased to a low level in China. However, there still exist high endemic areas. Thus, intervention needs to be applied in the high endemic areas and elder population. [ABSTRACT FROM AUTHOR]