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Low-fertility rate has been a common problem in many industrialized countries. To reverse the declining trend of new births, Chinese government gradually lifted its restrictions on the number of births per family, allowing for a household to have no more than two children. Little is known about the additional births or population increase contributed by the gradual relaxation of birth restrictions. To fill this gap, this quasi-experimental design study including data from 124 regions used the synthetic control method and controlled interrupted time series analysis to evaluate the differences in birth rates and rates of natural population increase between China and its synthetic control following implementation of the two-child policy from 2011 to 2020. A total of 123 regions were included in the control pool. Data collected during 1990–2010 were used to identify the synthetic China for each study outcome. The mean rate differences of birth rates and rates of natural increase between China and synthetic China after two-child policy implementation were 1.16 per 1000 population and 1.02 per 1000, respectively. These rate differences were distinguished from variation due to chance (one-sided pseudo- P -values: P for birth rates = 0.047, P for rates of natural increase = 0.020). However, there were statistically significant annual reductions in the pre–post trend of birth rates and rates of natural increase compared with those of controls of <0.340 per 1000 population per year [ P  = 0.007, 95% CI = (−0.584, −0.096)] and <0.274 per 1000 per year [ P  = 0.028, 95% CI = (−0.518, −0.031)]. The results suggested that lifting birth restrictions had a short-term effect on the increase in birth rates and rates of natural population increase. However, birth policy with lifting birth restrictions alone may not have sustained impact on population growth in the long run. [ABSTRACT FROM AUTHOR]

Background: The importance of the gut microbiota in maintaining bone homeostasis has been increasingly emphasized by recent research. This study aimed to identify whether and how the gut microbiome of postmenopausal women with osteoporosis and osteopenia may differ from that of healthy individuals. Methods: Fecal samples were collected from 27 individuals with osteoporosis (OP), 44 individuals with osteopenia (ON), and 23 normal controls (NC). The composition of the gut microbial community was analyzed by 16S rRNA gene sequencing. Results: No significant difference was found in the microbial composition between the three groups according to alpha and beta diversity. At the phylum level, Proteobacteria and Fusobacteriota were significantly higher and Synergistota was significantly lower in the ON group than in the NC group. At the genus level, Roseburia, Clostridia_UCG.014, Agathobacter, Dialister and Lactobacillus differed between the OP and NC groups as well as between the ON and NC groups (p < 0.05). Linear discriminant effect size (LEfSe) analysis results showed that one phylum community and eighteen genus communities were enriched in the NC, ON and OP groups, respectively. Spearman correlation analysis showed that the abundance of the Dialister genus was positively correlated with BMD and T score at the lumbar spine (p < 0.05). Functional predictions revealed that pathways relevant to amino acid biosynthesis, vitamin biosynthesis, and nucleotide metabolism were enriched in the NC group. On the other hand, pathways relevant to metabolites degradation and carbohydrate metabolism were mainly enriched in the ON and OP groups respectively. Conclusions: Our findings provide new epidemiologic evidence regarding the relationship between the gut microbiota and postmenopausal bone loss, laying a foundation for further exploration of therapeutic targets for the prevention and treatment of postmenopausal osteoporosis (PMO). [ABSTRACT FROM AUTHOR]

Climate change and urbanization intersect with escalating danger. Urban areas significantly contribute to climate change, which, in turn, poses severe threats to urban settings. The frequency and intensity of extreme events, like flooding and heat, are rising, with the need to enhance urban climate resilience (UCR) becoming more immediate. Scholarship tends to underrepresent general climate resilience in favor of specific hazards. This research seeks to contribute to the literature by exploring the case of Shanghai, China, discovering the mechanisms and characteristics of UCR governance, and examining how these outcomes are formed from a comparative gesture. The findings indicate that in Shanghai, 36.8% and 26.8% of climate resilience governance strategies are reflected in regional management and infrastructure construction led by the Water Affairs Bureau and the Meteorological Bureau. Furthermore, 30.6% of the strategies relate to the Water Affairs Bureau, showcasing a robust and integrated flood response. Meanwhile, 15.7% involve the Meteorological Bureau, boosting responses to high temperatures with better monitoring and early warning for increased flexibility and efficiency. Distinct governance processes for floods and extreme heat mirror these hazards' inherent characteristics and societal perceptions. With strong government willingness and support, Shanghai has rapidly enhanced its flood resilience capabilities within a brief timeframe. Conversely, addressing the emerging risk of extreme heat is still in the early stages of evaluation, due to the lack of a clear disaster-bearing system and identified responsible agents. This research suggests that the future of climate resilience governance in Shanghai may emphasize identifying the characteristics of critical climate-related risks, expanding social autonomy through grassroots self-governance, procuring economic backing from the central government, and applying the tool of urban spatial planning. [ABSTRACT FROM AUTHOR]

PSI is a sophisticated photosynthesis protein complex that fuels the light reaction of photosynthesis in algae and vascular plants. While the structure and function of PSI have been studied extensively, the dynamic regulation on PSI oligomerization and high light response is less understood. In this work, we characterized a high light-responsive immunophilin gene FKB20-2 (FK506-binding protein 20-2) required for PSI oligomerization and high light tolerance in Chlamydomonas (Chlamydomonas reinhardtii). Biochemical assays and 77-K fluorescence measurement showed that loss of FKB20-2 led to the reduced accumulation of PSI core subunits and abnormal oligomerization of PSI complexes and, particularly, reduced PSI intermediate complexes in fkb20-2. It is noteworthy that the abnormal PSI oligomerization was observed in fkb20-2 even under dark and dim light growth conditions. Coimmunoprecipitation, MS, and yeast 2-hybrid assay revealed that FKB20-2 directly interacted with the low molecular weight PSI subunit PsaG, which might be involved in the dynamic regulation of PSI-light-harvesting complex I supercomplexes. Moreover, abnormal PSI oligomerization caused accelerated photodamage to PSII in fkb20-2 under high light stress. Together, we demonstrated that immunophilin FKB20-2 affects PSI oligomerization probably by interacting with PsaG and plays pivotal roles during Chlamydomonas tolerance to high light. FKB20-2 interacts with PSI small subunit PsaG in thylakoid lumen, ensuring the accumulation of PSI complexes and influencing the high light tolerance of Chlamydomonas. [ABSTRACT FROM AUTHOR]