Search

Your search keyword '"Łukasz Wyrozżemski"' showing total 2 results
Start Over Author "Łukasz Wyrozżemski" Publication Year Range Last 10 years
2 results on '"Łukasz Wyrozżemski"'

1. Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.

Author

Ying Yao, Łukasz Wyrozżemski, Knut E A Lundin, Geir Kjetil Sandve, and Shuo-Wang Qiao

Subjects
Medicine, Science
Abstract

Gluten-specific CD4+ T cells drive the pathogenesis of celiac disease and circulating gluten-specific T cells can be identified by staining with HLA-DQ:gluten tetramers. In this first single-cell RNA-seq study of tetramer-sorted T cells from untreated celiac disease patients blood, we found that gluten-specific T cells showed distinct transcriptomic profiles consistent with activated effector memory T cells that shared features with Th1 and follicular helper T cells. Compared to non-specific cells, gluten-specific T cells showed differential expression of several genes involved in T-cell receptor signaling, translational processes, apoptosis, fatty acid transport, and redox potentials. Many of the gluten-specific T cells studied shared T-cell receptor with each other, indicating that circulating gluten-specific T cells belong to a limited number of clones. Moreover, the transcriptional profiles of cells that shared the same clonal origin were transcriptionally more similar compared with between clonally unrelated gluten-specific cells.

Published
2021
Full Text
View/download PDF

2. Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq

Author

Łukasz Wyrozżemski, Geir Kjetil Sandve, Shuo-Wang Qiao, Knut E.A. Lundin, and Ying Yao

Subjects
Cell signaling, Physiology, T-Lymphocytes, Cell, Gene Expression, Signal transduction, Immune Receptors, Biochemistry, Transcriptome, White Blood Cells, Animal Cells, Immune Physiology, Gene expression, Medicine and Health Sciences, RNA-Seq, Receptor, Innate Immune System, Multidisciplinary, Immune System Proteins, TCR signaling cascade, Effector, T Cells, Signaling cascades, Genomics, medicine.anatomical_structure, Medicine, Cytokines, Single-Cell Analysis, Cellular Types, Transcriptome Analysis, Research Article, Cell biology, Glutens, Science, Immune Cells, Immunology, Receptors, Antigen, T-Cell, Gastroenterology and Hepatology, Biology, digestive system, medicine, Genetics, Humans, Cell Lineage, Gene, Blood Cells, Biology and life sciences, Gene Expression Profiling, T-cell receptor, nutritional and metabolic diseases, Proteins, Computational Biology, Th1 Cells, Molecular Development, Genome Analysis, Molecular biology, digestive system diseases, T Cell Receptors, Celiac Disease, Gene Expression Regulation, Apoptosis, Immune System, Gluten, Developmental Biology
Abstract

Gluten-specific CD4+ T cells drive the pathogenesis of celiac disease and circulating gluten-specific T cells can be identified by staining with HLA-DQ:gluten tetramers. In this first single-cell RNA-seq study of tetramer-sorted T cells from untreated celiac disease patients blood, we found that gluten-specific T cells showed distinct transcriptomic profiles consistent with activated effector memory T cells that shared features with Th1 and follicular helper T cells. Compared to non-specific cells, gluten-specific T cells showed differential expression of several genes involved in T-cell receptor signaling, translational processes, apoptosis, fatty acid transport, and redox potentials. Many of the gluten-specific T cells studied shared T-cell receptor with each other, indicating that circulating gluten-specific T cells belong to a limited number of clones. Moreover, the transcriptional profiles of cells that shared the same clonal origin were transcriptionally more similar compared with between clonally unrelated gluten-specific cells.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results