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212 results on '"Amoh, Yasuyuki"'

3. Hair-follicle-associated pluripotent stem cells derived from cryopreserved intact human hair follicles sustain multilineage differentiation potential

Author

Obara, Koya, Tohgi, Natsuko, Mii, Sumiyuki, Hamada, Yuko, Arakawa, Nobuko, Aki, Ryoichi, Singh, Shree Ram, Hoffman, Robert M, and Amoh, Yasuyuki

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Engineering, Biomedical Engineering, Regenerative Medicine, Stem Cell Research, Adult, Aged, Cell Culture Techniques, Cell Differentiation, Cell Lineage, Cryopreservation, Gene Expression Regulation, Hair Follicle, Humans, Middle Aged, Pluripotent Stem Cells
Abstract

The bulge area of the hair follicle contains hair-follicle-associated pluripotent (HAP) stem cells. Here, we present effective cryopreservation procedures of the human hair follicle that preserve the differentiation potential of HAP stem cells. Whole hair follicles isolated from human scalp were cryopreserved by a slow-rate cooling medium and stored in liquid nitrogen. A careful thawing method was used to collect the upper parts of the human hair follicles which were cultured for four weeks in a Dulbecco's Modified Eagle's Medium with fetal bovine serum (FBS). Proliferating hair follicle cells were then shifted to DMEM/Ham's Nutrient Mixture F-12 medium without FBS and allowed to grow for one week. These proliferating cells were able to produce HAP stem cell colonies with multilineage differentiation capacity. They produced keratinocytes, smooth muscle cells, cardiac muscle cells, neurons and glial cells. Interestingly, these cryopreserved hair follicles produced pluripotent HAP stem cell colonies similar to fresh follicles. These findings suggest that the cryopreserved whole human hair follicle preserves the ability to produce HAP stem cells, which will enable any individual to preserve a bank of these stem cells for personalized regenerative medicine.

Published
2019

4. Hair-follicle associated pluripotent (HAP)-cell-sheet implantation enhanced wound healing in diabetic db/db mice.

Author

Hasegawa-Haruki, Ayami, Obara, Koya, Takaoka, Nanako, Shirai, Kyoumi, Hamada, Yuko, Arakawa, Nobuko, Aki, Ryoichi, Hoffman, Robert M., and Amoh, Yasuyuki

Subjects
KERATINOCYTE differentiation, WOUND healing, SKIN regeneration, GRANULATION tissue, VASCULAR endothelial cells, TISSUE wounds, MUSCLE cells, TYPE 2 diabetes
Abstract

Diabetes often results in chronic ulcers that fail to heal. Effective treatment for diabetic wounds has not been achieved, although stem-cell-treatment has shown promise. Hair-follicle-associated-pluripotent (HAP)-stem-cells from bulge area of mouse hair follicle have been shown to differentiate into keratinocytes, vascular endothelial cells, smooth muscle cells, and some other types of cells. In the present study, we developed HAP-cell-sheets to determine their effects on wound healing in type-2 diabetes mellitus (db/db) C57BL/6 mouse model. Flow cytometry analysis showed cytokeratin 15 expression in 64% of cells and macrophage expression in 3.6% of cells in HAP-cell-sheets. A scratch cell migration assay in vitro showed the ability of fibroblasts to migrate and proliferate was enhanced when co-cultured with HAP-cell-sheets. To investigate in vivo effects of the HAP-cell-sheets, they were implanted into 10 mm circular full-thickness resection wounds made on the back of db/db mice. Wound closure was facilitated in the implanted group until day 16. The thickness of epithelium and granulation tissue volume at day 7 were significantly increased by the implantation. CD68 positive area and TGF-β1 positive area were significantly increased; meanwhile, iNOS positive area was reduced at day 7 in the HAP-cell-sheets implanted group. After 21 days, CD68 positive areas in the implanted group were reduced to under the control group level, and TGF-β1 positive area had no difference between the two groups. These observations strongly suggest that the HAP-cell-sheets implantation is efficient to facilitate early macrophage activity and to suppress inflammation level. Using immuno-double-staining against CD34 and α-SMA, we found more vigorous angiogenesis in the implanted wound tissue. The present results suggest autologous HAP-cell-sheets can be used to heal refractory diabetic ulcers and have clinical promise. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Rat hair-follicle-associated pluripotent (HAP) stem cells can differentiate into atrial or ventricular cardiomyocytes in culture controlled by specific supplementation

Author

Takaoka, Nanako, primary, Yamane, Michiko, additional, Hasegawa, Ayami, additional, Obara, Koya, additional, Shirai, Kyoumi, additional, Aki, Ryoichi, additional, Hatakeyama, Hiroyasu, additional, Hamada, Yuko, additional, Arakawa, Nobuko, additional, Tanaka, Manabu, additional, Hoffman, Robert M., additional, and Amoh, Yasuyuki, additional

Published
2024
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16. Implanted hair-follicle-associated pluripotent (HAP) stem cells differentiating to keratinocytes, macrophages and endothelial cells accelerated cutaneous wound closure and suppressed scar formation in a mouse model

Author

Obara, Koya, primary, Shirai, Kyoumi, additional, Hamada, Yuko, additional, Arakawa, Nobuko, additional, Hasegawa, Ayami, additional, Takaoka, Nanao, additional, Aki, Ryoichi, additional, Hoffman, Robert M., additional, and Amoh, Yasuyuki, additional

Published
2022
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17. Chronic spinal cord injury functionally repaired by direct implantation of encapsulated hair-follicle-associated pluripotent (HAP) stem cells in a mouse model: Potential for clinical regenerative medicine

Author

Obara, Koya, primary, Shirai, Kyoumi, additional, Hamada, Yuko, additional, Arakawa, Nobuko, additional, Yamane, Michiko, additional, Takaoka, Nanako, additional, Aki, Ryoichi, additional, Hoffman, Robert M., additional, and Amoh, Yasuyuki, additional

Published
2022
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35. Gray‐edged line sign of scabies burrow.

Author

Ueda, Takashi, Katsura, Yuri, Sasaki, Azusa, Minagawa, Daisuke, Amoh, Yasuyuki, and Shirai, Kyoumi

Abstract

A scabies burrow is created by a mature female mite laying eggs through the stratum corneum, representing a kind of scabies eruption. We have noticed that the edges of the scabies burrow sometime appear as blackish‐gray lines. We named these lines the "gray‐edged line" sign, as a new feature of scabies burrows. The gray‐edged line sign has the following two tendencies: (i) it is rarely seen on the palm or sole; and (ii) when the burrow follows a curved course, the gray‐edged line often forms on the outer wall. Explaining the formation of this sign from clinical findings was difficult, so the aim of the present study was to elucidate the mechanisms underlying the gray‐edged line sign. This retrospective study involved collection of data from electronic medical records of patients treated for scabies in our department between April 2015 and February 2020. We treated 32 scabies patients, including 4 patients with the gray‐edged line sign. We analyzed clinical features, dermoscopy, histopathology and special stains. Fontana‐Masson staining showed melanin staining in three parts: feces; some keratinocytes around the scabies burrows; and the mouth and legs of the scabies mite. The gray‐edged line sign appears to represent mite feces containing melanin. [ABSTRACT FROM AUTHOR]

Published
2021
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36. A potential significance of circ_0024169 down regulation in angiosarcoma tissue

Author

Nakashima, Satoko, primary, Jinnin, Masatoshi, additional, Ide, Maho, additional, Kajihara, Ikko, additional, Igata, Toshikatsu, additional, Harada, Miho, additional, Masuguchi, Shinichi, additional, Fukushima, Satoshi, additional, Masuzawa, Mikio, additional, Masuzawa, Mamiko, additional, Amoh, Yasuyuki, additional, and Ihn, Hironobu, additional

Published
2019
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43. Implanted hair-follicle-associated pluripotent (HAP) stem cells encapsulated in polyvinylidene fluoride membrane cylinders promote effective recovery of peripheral nerve injury

Author

Yamazaki, Aiko, primary, Obara, Kohya, additional, Tohgi, Natsuko, additional, Shirai, Kyoumi, additional, Mii, Sumiyuki, additional, Hamada, Yuko, additional, Arakawa, Nobuko, additional, Aki, Ryoichi, additional, Hoffman, Robert M., additional, and Amoh, Yasuyuki, additional

Published
2017
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