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1. Thiamphenicol and florfenicol combination in Nile tilapia: Simultaneous detection and quantification in plasma and muscle plus skin samples, and pharmacokinetics following single oral administration

Author

Assane, Inácio Mateus, Damaceno, Marina Alves, Campanharo, Sarah Chagas, da Silva, Agnaldo Fernando Baldo, de Sousa, Elielma Lima, do Vale Oliveira, André, de Abreu Reis Ferreira, Daniel, Kotzent, Suzana, de Jesus, Raphael Barbetta, da Paz, Deborah Jacob Freire, Paschoal, Jonas Augusto Rizzato, and Pilarski, Fabiana

Published
2023
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9. Residue Depletion Profile and Estimation of Withdrawal Period for Sulfadimethoxine and Ormetoprim in Edible Tissues of Nile Tilapia (Oreochromis sp.) on Medicated Feed

Author

de Freitas, Lucas Victor Pereira, primary, da Mota Júnior, Carlos Augusto Alvarenga, additional, Damaceno, Marina Alves, additional, Silveira, Juliana Grell Fernandes, additional, Portela, Ana Carolina Vellosa, additional, Campanharo, Sarah Chagas, additional, da Silva, Agnaldo Fernando Baldo, additional, Assane, Inácio Mateus, additional, Pilarski, Fabiana, additional, Sasanya, James Jacob, additional, and Paschoal, Jonas Augusto Rizzato, additional

Published
2023
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13. Enterogyrus spp. (Monogenea: Ancyrocephalinae) and Aeromonas jandaei co‐infection associated with high mortality following transport stress in cultured Nile tilapia.

Author

Assane, Inácio Mateus, Prada‐Mejia, Karen Dayana, Gallani, Sílvia Umeda, Weiser, Natasha Fernandes, Valladão, Gustavo Moraes Ramos, and Pilarski, Fabiana

Subjects
*NILE tilapia, *MONOGENEA, *MIXED infections, *AEROMONAS, *GASTRIC mucosa, *FISH stocking
Abstract

Monogenean infection of the internal organs is extremely rare when compared to external infections. This study describes mass mortality of Nile tilapia (Oreochromis niloticus L.) originating from co‐infection with Enterogyrus spp. and Aeromonas jandaei following transport stress. The first fish deaths occurred on day 1 post‐transport, while cumulative mortality reached approximately 90% by day 10 post‐stocking. An atypical amount of pale (whitish) faeces floating on the surface of the water as well as typical clinical signs of motile Aeromonas septicemia, were reported. Adult monogeneans and countless eggs of monogeneans were found in the stomachs and the intestines of both moribund and dead fish, respectively. Two strains of A. jandaei were isolated from the kidneys. Scanning electron microscope microphotographs of the stomach revealed the presence of numerous monogeneans penetrating deep into the gastric tissue, and diffuse lesions filled with bacilliform bacteria. Histopathological examination showed multifocal eosinophilic infiltrate, gastric gland and epithelial necrosis with sloughed necrotic debris in the lumen. This is the first report of co‐infection by Enterogyrus spp. and A. jandaei in Nile tilapia and the first report of Enterogyrus coronatus, Enterogyrus foratus, and Enterogyrus malbergi parasitizing tilapia in Brazil. These findings indicate that synergic co‐infection by Monogenean stomach parasites (E. coronatus, E. foratus, and E. malbergi) and A. jandaei may induce high mortalities in tilapia following transport stress. [ABSTRACT FROM AUTHOR]

Published
2022
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17. Atividade antimicrobiana do tianfenicol sobre bactérias patogênicas de peixes

Author

Assane, Inácio Mateus, Universidade Estadual Paulista (Unesp), and Pilarski, Fabiana [UNESP]

Subjects
tianfenicol, sinergismo, thiamphenicol, bactérias, florfenicol, Nile tilapia, synergism, Antimicrobial, Aeromonas, bacteria, tilápia-do-Nilo, Antimicrobiano
Abstract

Submitted by Inácio Mateus Assane null (inaciomateusassane@gmail.com) on 2018-03-13T16:40:15Z No. of bitstreams: 1 Versão Final da Dissertação_Inácio Mateus Assane.pdf: 2500612 bytes, checksum: e7b1dcaa29bfec4105b34a055b4a403d (MD5) Approved for entry into archive by Neli Silvia Pereira null (nelisps@fcav.unesp.br) on 2018-03-16T17:46:12Z (GMT) No. of bitstreams: 1 assane_im_me_jabo.pdf: 2500612 bytes, checksum: e7b1dcaa29bfec4105b34a055b4a403d (MD5) Made available in DSpace on 2018-03-16T17:46:12Z (GMT). No. of bitstreams: 1 assane_im_me_jabo.pdf: 2500612 bytes, checksum: e7b1dcaa29bfec4105b34a055b4a403d (MD5) Previous issue date: 2018-02-28 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Os surtos de bacterioses na piscicultura são imprevisíveis, por essa razão é necessário dispor de opções terapêuticas eficazes para reduzir a morbidade e mortalidade. Para que a terapia seja eficaz, a escolha do antimicrobiano deve se basear nas informações farmacocinéticas do fármaco no hospedeiro, nas condições de produção e na sensibilidade do patógeno. No Brasil, atualmente só dois antimicrobianos são legalizados para uso na aquicultura, a oxitetraciclina (OTC) e o florfenicol (FFC), os quais muitas vezes são ineficazes. Destes, somente o FFC é autorizado para tratar bacterioses em tilápia-do-Nilo, a espécie mais produzida no país. Este cenário nos levou a investigar a atividade antimicrobiana do tianfenicol (TAF) contra as principais bactérias patogênicas de peixes cultivados no Brasil. Foi determinada a concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM) do TAF para 49 cepas isoladas de surtos de bacteriose ocorridos no período 2011 a 2017 em três estados. Adicionalmente, avaliou-se a atividade antimicrobiana do TAF (G1: 10 mg/kg), do FFC (G2: 10 mg/kg) e da combinação do TAF com o FFC (G3: TAF+FFC: 5 + 2,5 mg/kg e G4: 2,5 + 1,25 mg/kg) no tratamento de aeromonose em tilápia-do-Nilo experimentalmente infectadas com Aeromonas hydrophila. Como resultado do estudo in vitro, mais da metade das cepas testadas foram sensíveis ao TAF, sendo que os gêneros Aeromonas, Lactococcus e Vibrio foram os mais sensíveis. No estudo in vivo, o TAF foi eficaz no tratamento da aeromonose em tilápia-do-Nilo, tanto isolado como combinado. Os grupos G1, G2, G3, G4 e o controle apresentaram uma taxa de sobrevivência de 88,33 ± 11,55%; 70 ± 10%; 73,33 ± 11,55%; 85,93 ± 5,13% e 3,33 ± 5,77%, respectivamente, sendo todos diferentes do controle (p < 0,001). Os resultados do estudo sugerem que o TAF pode ser uma boa opção de tratamento de aeromonose, lactococose e vibriose em peixes. Além disso, a combinação do TAF com o FFC apresentou um efeito sinérgico, mostrando que é possível reduzir a quantidade de antimicrobiano e obter o mesmo efeito terapêutico. Outbreaks of bacterial diseases in fish farm are unpredictable; therefore, it is necessary to have effective therapeutic options to reduce the morbidity and mortality. For effective therapy, the anticipated pharmacokinetics and antibacterial activity of the antimicrobial in the target fish species under the given conditions must be considered before decide which antimicrobial to use. In Brazil, only two antimicrobials are approved for use in aquaculture, oxytetracycline (OTC) and florfenicol (FFC), which are often innefective. Moreover, only FFC is approved for use to treat bacterial diseases in Nile tilapia, the main specie in Brazil. This scenario led us to investigate the antibacterial activity o thiamphenicol (TAP) against the main bacteria infecting fishes in Brazil. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of TAP were determined for 49 strains isolated from diseases outbreaks registered during 2011 – 2017 in three States. Additionally the antibacterial activity of TAP (10 mg kg-1), FFC (10 mg kg-1) and TAF combined with FFC (G3: TAF+FFC: 5 + 2.5 mg kg-1 e G4: 2.5 + 1.25 mg kg-1) against Aeromonas hydrophila in Nile tilapia aeromoniosi models were evaluated. More than half of the tested strains were in vitro sensitive to TAP, being TAP highly potent against Aeromonas, Lactococcus, and Vibrio. The in vivo therapy with TAP alone and combined with FFC was effective to treat aeromoniosis in Nile tilapia. Groups G1, G2, G3, G4 and control had survival rate of 88.33 ± 11.55%; 70 ± 10%; 73.33 ± 11.55%; 85.93 ± 5.13% e 3.33 ± 5.77%, respectively, all being different from the control (p < 0,001). These findings suggest the potential of TAP as a treatment option for aeromoniosis, lactococcosis and vibriosis. The combination of TAF and FFC has a synergy effect, showing that it is possible to reduce the amount of antimicrobial while maintaining the therapeutic effectiveness. 190049/2015-4

Published
2018
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21. Detection and Analysis of Florfenicol Residues and Metabolites in Nile Tilapia (Oreochromis niloticus) Tissues Post‐Oral Administration in Tropical Waters.

Author

Queiroga, Anna Paula R., Souza, Gabriela F. P., Paschoal, Jonas Augusto R., Salles, Airton Gonçalves Jr, Schloter, Michael, Assane, Inácio Mateus, Pilarski, Fabiana, Gotardo, André Tadeu, Górniak, Silvana Lima, and Rath, Susanne

Subjects
*NILE tilapia, *ORAL drug administration, *FISH fillets, *WATER temperature, *TILAPIA
Abstract

ABSTRACT Water temperature is a critical environmental parameter that significantly influences fish metabolism. This study assessed the metabolism of florfenicol (FF) in tilapia (Oreochromis niloticus) at water temperatures typical of tropical and subtropical regions. Fish were treated with FF by oral administration of a dose of 10 mg kg−1 bw for 10 consecutive days. Fish fillet, liver, and kidney were sampled during the treatment phase (1, 5, and 10 days) and posttreatment (1, 2, 3, and 5 days after the last FF administration). FF, florfenicol amine (FFA), monochloro florfenicol (FFCl), and florfenicol alcohol (FFOH) were determined in the sampled tissues using a validated LC–LC–MS/MS method. The highest FF, FFA, and FFOH concentrations were determined on day 5 during the treatment phase. For FF, the concentration order is kidney > liver > fillet, while for the metabolites FFOH and FFA, the order is liver > kidney > fillet. In fillet and liver, the concentrations of FFOH were higher than the FFA concentrations, indicating that FFOH was the primary metabolite in these tissues. FFCl was only quantified at concentrations lower than 90 μg kg−1 in all tissues. The results indicated that FF can be readily absorbed and rapidly eliminated in tilapia cultivated in warm water environments. This study revealed FFOH as the primary and most persistent metabolite in tilapia farmed in warm water, followed by FFA. [ABSTRACT FROM AUTHOR]

Published
2024
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