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The current work aimed to analyze, morphologically, statistically and molecularly, oocysts shed from plumbeous pigeons Patagioenas plumbea Vieillot, 1818 from a locality at 2197m of altitude near the Agulhas Negras peak, the highest point of the State of Rio de Janeiro, southeastern Brazil. The oocysts were extremely polymorphic, being subspheroidal, ovoidal or ellipsoidal, in addition to having the random presence/absence of characteristic features associated with the oocyst wall, such as micropyle, micropyle cap, lateral micropyle and outer veil/rough wall. Linear regression confirmed the extreme polymorphism of oocysts, showing that if all combinations of taxonomic characters in oocysts (morphotypes) were overestimated, 19 different species could be identified/described. In contrast, the means comparison analysis between oocysts with presence/absence of characteristic features and the histograms showed equivalences and regularity in the distribution in the classes of measures, which indicate the presence of a single species in the measured oocysts. Molecular analyzes was performed from the isolation of individual oocysts of different morphotypes, which had their genetic material extracted, amplified and sequenced in 4 non-overlapping loci in cox1, cox3 genes and fragments of small and large subunit rDNA of mitochondrial DNA. The sequences were 100% identical between the morphotypes, with the exception of a very small divergence observed at the locus that partially covers the cox3 gene. The phylogenetic analysis was inconclusive for the locus within cox1 gene traditionally used for eimeriid coccidians, however the other loci should have a promising future for phylogenetic studies when more sequences for the same genic regions are deposited in GenBank. Finally, the multifactorial analysis of the current work supported that the polymorphic oocysts shed from P. plumbea are a single species, which was named as Eimeria patagioenasae, making this the twenty-second eimerian description from Columbiformes.

Isospora coerebae Berto, Flausino, Luz, Ferreira and Lopes, 2010 Host: Bananaquit Coereba flaveola (Linnaeus, 1758) (Passeriformes: Thraupidae: Coerebinae) Locality: Private Natural Heritage Reserve of Porangaba (22°48’29.83’’S; 43°49’38.77’’W), Municipality of Itaguaí, State of Rio de Janeiro, Southeastern Brazil. Specimens: Photomicrographs are deposited and available (http://r1.ufrrj.br/labicoc/colecao.html) in the Parasitology Collection of the Laboratório de Biologia de Coccídios, at UFRRJ, under repository number 115/2021. Photovouchers of the host specimens are deposited in the same collection. Representative DNA sequence: DNA amplification of the COI1 and COI2 genic regions showed clear bands around ~250 bp and ~650 bp, respectively. Representative sequences were deposited in the GenBank database under the accession numbers: OK194671 (COI1); and OK194672 (COI2). Site of infection: Unknown. Prevalence: 100% (1/1). Sporulation: Exogenous. All oocysts were passed in the feces unsporulated and were fully sporulated by day 7 in K 2 Cr 2 O 7 solution at room temperature (20–25°C). Morphology (Fig 1 A-F): Oocyst (n = 15) sub-spherical, 22−27 × 22−25 (24.4 × 22.9); length/width (L/W) ratio 1.0−1.1 (1.07). Wall bi-layered, smooth outer wall about 2/3 total thickness, 1.5−1.8 (1.7). Micropyle, polar granules and oocyst residuum absent. Sporocyst elongate ovoidal, 16−19 × 10−11 (17.6 × 10.5); L/W ratio 1.6−1.8 (1.67). Stieda body present, prominent and rounded, 1.3−1.5 × 1.9−2.3 (1.4 × 2.1). Sub-Stieda body present, short and wide, 1.0−1.6 × 3.1−3.7 (1.3 × 3.5). Para-Stieda body absent. Crystalloid body sometimes present in the center of the sporocyst. Sporocyst residuum present, composed of many scattered granules of different sizes. Sporozoites vermiform, with a prominent posterior refractile body. Remarks: The oocysts recovered from the bananaquit in the current work are morphologically equivalent to the original description of I. coerebae, which have a typical and easily identifiable morphology, mainly in the Stieda and Sub-Stieda bodies (Berto et al. 2010). Table 1 compares the morphology and morphometry of the oocysts from the current work with those from the original description of I. coerebae. The typical morphology of the Stieda and Sub-Stieda bodies of I. coerebae are the basis of differentiation from another congeneric species, Isospora cagasebi Berto, Flausino, Luz, Ferreira and Lopes, 2008, which has the same host- and locality-types: C. flaveola in the Marambaia Island (Berto et al. 2008) (Table 1). However, in the current work no oocyst of I. cagasebi was observed, which, despite being similar in some characteristic features to I. coerebae, their sporocysts have knob-like Stieda body and prominent Sub-Stieda body, while I. coerebae has rounded Stieda body and short and wide Sub-Stieda body (Berto et al. 2010). Phylogenetic analysis: Phylogenetic analysis included sequences from coccidians available in GenBank (Figs. 2; 3). Toxoplasma gondii (Nicolle and Manceaux, 1908) was used as the outgroup. In the phylogenetic analysis based on the COI1 gene (Fig. 2), I. coerebae was recovered in a clade with the highest similarity of 99% with Isospora sepetibensis Berto, Flausino, Luz, Ferreira and Lopes, 2008 from black-goggled tanagers Trichothraupis melanops (Vieillot, 1818) of the Itatiaia National Park, which is located in the mountainous region in the interior of Southeastern Brazil. In the COI2 based phylogenetic analysis (Fig. 3), I. coerebae sat in a large clade with Isospora spp. from passerines of North America, Europe and Asia, with the highest similarity of 98% with Isospora greineri Hafeez, Stasiak, Delnatte, El-Sherry, Smith and Barta, 2014 from superb starlings Lamprotornis superbus Rüppell, 1845 at the Toronto Zoo, Toronto, Canada., Published as part of Ortúzar-Ferreira, Carlos Nei, Andrade, Lucas De Assis S., Genovezoliveira, Jhon Lennon, Oliveira, Mariana S., Mello, Ericson R., Cardozo, Sergian V., Oliveira, Águida A., Lima, Viviane M., Ferreira, Ildemar & Berto, Bruno P., 2022, Molecular identification of Isospora coerebae Berto, Flausino, Luz, Ferreira & Lopes, 2010 (Chromista: Miozoa: Eimeriidae) from the bananaquit Coereba flaveola (Linnaeus, 1758) (Passeriformes: Thraupidae: Coerebinae) from Brazil, pp. 83-91 in Zootaxa 5168 (1) on pages 85-86, DOI: 10.11646/zootaxa.5168.1.7, http://zenodo.org/record/6876923, {"references":["Berto, B. P., Flausino, W., Luz, H. R., Ferreira, I. & Lopes, C. W. G. (2010) Isospora coerebae n. sp. (Apicomplexa: Eimeriidae) from the bananaquit Coereba flaveola (Passeriformes: Coerebidae) in South America. Revista Brasileira de Parasitologia Veterinaria, 20 (1), 22 - 26. https: // doi. org / 10.1590 / S 1984 - 29612011000100005"]}

Ortúzar-Ferreira, Carlos Nei, Andrade, Lucas De Assis S., Genovezoliveira, Jhon Lennon, Oliveira, Mariana S., Mello, Ericson R., Cardozo, Sergian V., Oliveira, Águida A., Lima, Viviane M., Ferreira, Ildemar, Berto, Bruno P. (2022): Molecular identification of Isospora coerebae Berto, Flausino, Luz, Ferreira & Lopes, 2010 (Chromista: Miozoa: Eimeriidae) from the bananaquit Coereba flaveola (Linnaeus, 1758) (Passeriformes: Thraupidae: Coerebinae) from Brazil. Zootaxa 5168 (1): 83-91, DOI: https://doi.org/10.11646/zootaxa.5168.1.7

Isospora coerebae Berto, Flausino, Luz, Ferreira & Lopes, 2010 is a coccidian protozoan described from bananaquits Coereba flaveola (Linnaeus, 1758), on Marambaia Island, which is located on the southeastern Brazilian coast. In this current work, I. coerebae is identified from C. flaveola in a protected area close to Marambaia Island, but on the mainland, establishing a new location of parasitism, in addition to providing a preliminary genotypic characterization via sequencing of two regions of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Its oocysts are sub-spherical, 24.4 × 22.9 μm, with smooth, bilayered wall, ~1.7 μm thick. Micropyle, polar granules and oocyst residuum are absent. Sporocysts are elongate ovoidal, 17.6 × 10.5 μm. Stieda body prominent and rounded and sub-Stieda body short and wide. Sporocyst residuum is composed of scattered granules of different sizes. Sporozoites are vermiform with a prominent posterior refractile body. The oocysts of the current work are morphologically equivalent to the original description of I. coerebae, which have a typical and easily identifiable morphology, mainly in the Stieda and Sub-Stieda bodies. The two sequenced gene regions of the COI gene approximated I. coerebae to Isospora spp. from Southeastern Brazil, but also from Isospora spp. from passerines of North America, Europe and Asia. Although there is a small overlap between the two genic regions sequenced in the current work, it is estimated that the longer COI sequence, which was recently designed and still not widespread, should show better phylogenetic results in the future.

Neste artigo pretende-se mostrar uma investigação que vem sendo feita em escolas de formação profissional brasileiras com o uso de Células de Inovação (CI). As CI's são grupos de estudantes envolvidos com a tarefa de solucionar um problema concreto da de sua escola ou bairro utilizando-se de conhecimentos técnico-científicos. Procurou-se investigar, através de uma metodologia etnográfica, como esses alunos aprendem os conteúdos de ciência ao solucionar seu problema e se a inovação é algo que possa ser aprendido. As situações trabalhados são problematizadas numa perspectiva CTS e, em seguida, são discutidas soluções. A implementação das melhores soluções gera nova discussão CTS sobre seu impacto. O trabalho aqui descrito cobre apenas uma célula. Pretende-se que a partir de 2010 que a investigação estenda-se para várias células trabalhando em rede.

Background: Osteoarthritis (OA) is considered the third most prevalent chronic non-communicable disease in the Brazilian population, being responsible for a high rate of physical disability and reduced quality of life. Little has been discussed about the social factors associated with this health condition. This study aimed to analyze the social factors associated with OA in the Brazilian population. Methods: This is a cross-sectional study based on data from the 2013 National Health Survey in Brazil with OA as its main outcome. Gender, age, body weight, usual activities, physical activity, self-perceived health and diagnosis of depression were analyzed as independent variables. Descriptive and inferential statistics were used. Poisson multiple regression was performed, and the prevalence ratio (PR) and confidence interval (CI) of 95% were calculated using a significance level of 5% (p≤0.05). Results: A total of 60,202 individuals of both genders took part in this study and the OA prevalence was 6.4%. The individual factors associated with a higher prevalence of OA were female gender (PR = 2.09; CI = 1.95-2.25), age over 35 years (PR = 2.88; CI = 2.57-3.24) and excess body weight (PR = 1.61; CI = 1.25-2.07). The presence of OA showed an association with lower performance of usual activities (PR = 1.61; CI = 1.50-1.73) and self-perceived health as very poor (PR = 3.96; CI = 3.31-4.72). In addition, it was associated with a higher prevalence of mental illnesses such as depression (PR = 1.77; CI = 1.64-1.90). Conclusion: Social and modifiable factors which are associated with a higher prevalence of osteoarthritis can be controlled through incentive measures such as social participation and physical activity.

Isospora brasilsatoae Oliveira and Berto n. sp. Type host: Yellow-legged thrush Turdus flavipes (Vieillot, 1818) (Aves: Passeriformes: Turdidae). Other hosts: Pale-breasted thrush Turdus leucomelas Vieillot, 1818 (Aves: Passeriformes: Turdidae); creamybellied thrush Turdus amaurochalinus Cabanis, 1850 (Aves: Passeriformes: Turdidae). Type locality: Parque Nacional do Itatiaia (22°26'57"S, 44°36'25"W), Southeastern Brazil. Other localities: Cacaria (22°42'51"S, 43°50'38"W) and Guapimirim (22°31'10"S, 43°00'36"W), also from Southeastern Brazil. Type-material: Photosyntypes, line drawing and oocysts recovered from T. flavipes in 2.5% K 2 Cr 2 O 7 solution (Williams et al. 2010) are deposited at the Museu de Zoologia at the Universidade Federal Rural do Rio de Janeiro, Brazil, under accession number MZURPTZ2018007. Photomicrographs are also deposited and available (http:// r1.ufrrj.br/labicoc/colecao.html) in the Parasitology Collection of the Laboratório de Biologia de Coccídios, at UFRRJ, under repository number P-90/2018. Photographs of the type-host specimen (symbiotype) are deposited in the same collection. ZooBank registration: To comply with the regulations set out in article 8.5 of the amended 2012 version of the International Code of Zoological Nomenclature (ICZN, 2012), details of the new species have been submitted to ZooBank. The Life Science Identifier (LSID) for Isospora brasilsatoae is urn:lsid:zoobank.org:act: 6C3FEE82- FC9C-4A58-8C17-1713532F03F3. Site of infection: Unknown. Prevalence: Four of 10 (40%). Sporulation: Exogenous. All oocysts were passed in the feces unsporulated and were fully sporulated by day 7 in K 2 Cr 2 O 7 solution at room temperature (20–25°C). Etymology: The specific name is derived from the family name of a Brazilian parasitologist Dr Marília de Carvalho Brasil Sato, given in her honor for her contribution to the study of taxonomy and ecology of parasites. Description (Figs 1; 2 A–C). Oocyst (n = 15) ovoidal to pyriform, 25-30 × 19-23 (26.8 × 21.1); length/width (L/W) ratio 1.2-1.4 (1.27). Wall bi-layered, delicate, 1.4-1.8 (1.6) thick, outer layer smooth, c. 2/3 of total thickness. Micropyle present, without micropyle cap, wrinkles or invagination of the in inner layer perceptible. Oocyst residuum absent, but 1 to 3 rounded polar granules are present. Sporocyst elongate ovoidal with tapered anterior end (where it is Stieda/sub- Stieda complex), 17-19 × 10-11 (18.1 × 10.5); L/W ratio 1.6-1.9 (1.72). Stieda body present, knob-like, 2.0 high × 2.0 wide. Sub-Stieda body present, trapezoidal, 1.5 high × 3.5 wide. Para-Stieda body absent. Sporocyst residuum present, as a distinctly sub-spherical body consisting of numerous small granules that appear to be membranebounded, 6–9 (7.5). Sporozoites vermiform, with anterior and posterior refractile bodies and striations. Remarks: Isospora brasilsatoae is different from other coccidian species of Turdidae of the New World on several aspects (Table 1). It is the only one to have a pyriform shape of the oocysts. It has a micropyle, which is a characteristic present only in the oocysts of Isospora albicollis Lainson and Shaw, 1989, Isospora sabiai Pinho, Rodrigues, Silva, Lopes, Oliveira, Ferreira, Cardozo, Luz, Ferreira, Lopes and Berto, 2017 and Isospora machadoae Pinho, Silva, Rodrigues, Lopes, Oliveira, Luz, Ferreira, Lopes and Berto, 2018 (Lainson & Shaw 1989; Pinho et al. 2017a; 2017b; 2018). From these three, but also from the others, I. brasilsatoae can be easily differentiated by having up to three rounded polar granules, sporocysts elongate ovoidal (L/W ratio> 1.6), sub- Stieda body trapezoidal, and compact residuum.

Made available in DSpace on 2019-09-05T00:54:56Z (GMT). No. of bitstreams: 1 MadalenaPostharvest.pdf: 719026 bytes, checksum: 0ddb3a2beb00d030e14be9f9bf1ec046 (MD5) Previous issue date: 2019

Submitted by Nadir Basilio (nadirsb@uninove.br) on 2017-03-31T14:29:31Z No. of bitstreams: 1 Diego Nogueira Rafael.pdf: 940524 bytes, checksum: 7b50bcecd94daa3d3e48a730485ad712 (MD5) Made available in DSpace on 2017-03-31T14:29:31Z (GMT). No. of bitstreams: 1 Diego Nogueira Rafael.pdf: 940524 bytes, checksum: 7b50bcecd94daa3d3e48a730485ad712 (MD5) Previous issue date: 2016-12-15 Currently the Brazilian health system is complex, the constitution guarantee of care to the health care of all individuals within the national territory, and the State through the Unified Health System (SUS) is responsible for this difficult task. In this context, where the state is unable to serve the entire population, private organizations have taken part in this market, offering health plans and private assistance, which is called supplementary health. In this context, the themes studied are the psychological reactance that means the negative emotional reaction generated by the total or partial lack of freedom or the expectation of freedom of an individual who once got it and the health plan user???s satisfaction. User satisfaction and psychological reactance are topics exhaustively studied by the organizations, being one of the preferred themes in the study of consumer behavior, evidently being relevant because these studies allow the strategic analysis of the companies with more accurate data, resulting in greater profitability and success of these institutions. The study was conducted by means of a quantitative method, based on the satisfaction and psychological reactance of the users of the supplementary health system with the modeling of structural equations using the SmartPLS system, in order to test the hypotheses constructed based on the theories studied. The results obtained are in agreement with the theory of reactance and satisfaction, confirming the hypotheses proposed, there being a negative relation between both for the studied sample, the lower the reactance level observed, the higher the satisfaction level declared by the users. The conclusion of the study suggests as a contribution to the practice that managers approach the relationship between health plans and their users, creating programs to prevent diseases, combat sedentarism, chronic diseases and improve the quality of life of users, obtaining a participation collaborative of both. O sistema de sa??de brasileiro ?? complexo e a constitui????o garante o atendimento e assist??ncia ?? sa??de de todo o indiv??duo dentro do territ??rio nacional, devendo o Estado, por meio do Sistema ??nico de Sa??de (SUS), ser o respons??vel por essa dif??cil tarefa. Em um cen??rio no qual o Estado n??o consegue atender toda a popula????o, as organiza????es privadas tomaram para si parte desse mercado, oferecendo planos de sa??de e assist??ncia privada, os quais constituem o que ?? chamado de sistema de sa??de suplementar. Diante desse contexto, o tema deste trabalho ?? o estudo da reat??ncia psicol??gica, que ?? a rea????o emocional negativa gerada pela falta total ou parcial da liberdade ou da expectativa de liberdade de um indiv??duo que outrora a obtinha, e a satisfa????o dos usu??rios de planos de sa??de. A satisfa????o dos usu??rios e a reat??ncia psicol??gica s??o temas exaustivamente estudados pelas organiza????es, sendo um dos preferidos no estudo do comportamento do consumidor, sendo esse movimento relevante pelo fato de tais estudos possibilitarem a an??lise estrat??gica das empresas, trazendo dados mais precisos, resultando em maior lucratividade e sucesso dessas institui????es. O estudo foi conduzido por meio de m??todo quantitativo, fundamentado para analisar a satisfa????o e a reat??ncia psicol??gica dos usu??rios do sistema de sa??de suplementar por meio da Modelagem de Equa????es Estruturais, usando-se o software SmartPLS, com objetivo de testar as hip??teses constru??das com base nas teorias estudadas. Os resultados obtidos v??o ao encontro da teoria da reat??ncia e satisfa????o, confirmando as hip??teses propostas, havendo uma rela????o negativa entre ambas para a amostra estudada. Assim, quanto menor o n??vel da reat??ncia observada, maior o n??vel de satisfa????o declarada pelos usu??rios. A conclus??o do estudo sugere, como contribui????o para a pr??tica, que os gestores aproximem a rela????o dos planos de sa??de com seus usu??rios, criando programas para a preven????o de doen??as, combate ao sedentarismo, doen??as cr??nicas e melhoraria da qualidade de vida dos usu??rios, obtendo uma participa????o colaborativa de ambos.

Submitted by Nadir Basilio (nadirsb@uninove.br) on 2017-01-27T12:50:48Z No. of bitstreams: 1 Luis Renato Rotta Messias.pdf: 317202 bytes, checksum: 67360bd315784a5271d1c8027f6e9183 (MD5) Made available in DSpace on 2017-01-27T12:50:48Z (GMT). No. of bitstreams: 1 Luis Renato Rotta Messias.pdf: 317202 bytes, checksum: 67360bd315784a5271d1c8027f6e9183 (MD5) Previous issue date: 2016-02-12 Chemotherapy is still a main way to fight cancer and consequently, the demand for its treatment accompanies increased incidence and prevalence of disease. Provided most recommended type of therapy, chemotherapy is responsible for greatest impact on treatment cost. In this sense, this research proposes a method that helps management of chemotherapy schedule, improving issues related to logistics characteristics and time constraints so that treatment becomes more effective. From January 2012 to December 2013, data were collected relating production, costs of major inputs, loss of prepared medicines and chemotherapies relationship conducted with inpatients and outpatients, all related to proposed change in the management model of Oncology sector from a public hospital located at S??o Paulo state. As usual flow consisted of patient medical examination, with treatment prescribed by doctor, the new proposed protocol determined that the schedule no longer take place by the medical action, but by pharmacist, in the sense that he is professional manager, capable to accurately assess variables such as time, cost, competition for resources and optimization of infusion bags. As a result, the study showed increased number of outpatients and decrease number of inpatients, which generates cost savings. There was fourfold increase in total number of patients seen per year, with financial resources invested remaining stable. This study showed that new approaches to follow the flow model proposed here, would optimize resources, serving a larger number of patients, maintaining quality of processes, confidentiality of information, and saving costs and inputs. Furthermore, it was shown to be more effective multi scheduling, in which the physician says the protocol that will be used, the time will be used, and whether the patient is able to use, but with regard to the management of competing facilities as beds, interaction, armchair, chemotherapy or surgical room, a professional liability being with a logistics vision of the whole, in which case, a nurse or pharmacist A quimioterapia ainda ?? a principal forma de combate ao c??ncer e, consequentemente, a demanda por este tipo de tratamento acompanha o aumento da incid??ncia e preval??ncia da doen??a. Na condi????o de modalidade terap??utica mais preconizada, a quimioterapia ?? respons??vel pelo maior impacto no custo do tratamento. Nesse sentido, este trabalho prop??e um m??todo que auxilie a gest??o da agenda de tratamento quimioter??pico, melhorando quest??es relacionadas ??s caracter??sticas log??sticas e ??s limita????es de tempo para que o tratamento se torne mais efetivo. No per??odo de janeiro de 2012 a dezembro de 2013 foram coletados dados relacionados com a produ????o, custo dos insumos mais importantes, perdas de medicamentos preparados e rela????o de quimioterapias realizadas junto a pacientes internados e ambulatoriais, todos relacionados ?? mudan??a no modelo de gest??o do setor de Oncologia de um hospital p??blico do Estado de S??o Paulo. Enquanto o fluxo habitual consistia em o paciente passar em consulta m??dica, cabendo a este profissional a prescri????o do tratamento, o protocolo proposto neste estudo determinou que o agendamento n??o mais ocorreria pela a????o do m??dico, e sim do farmac??utico, pois este ?? gestor, profissional capaz de avaliar com precis??o vari??veis como tempo, custo, competi????o pelos recursos e otimiza????o da gest??o das bolsas de infus??o. Como resultados, o estudo demonstrou aumento do n??mero de pacientes ambulatoriais e diminui????o no n??mero de pacientes internados, o que gera redu????o de custos. Houve aumento de quatro vezes no total de pacientes atendidos por ano, com o valor investido mantendo-se est??vel. O presente estudo demonstrou que novas abordagens que sigam o modelo de fluxo aqui proposto otimizar??o os recursos, atendendo uma maior parcela de pacientes, mantendo a qualidade dos processos, o sigilo das informa????es, e poupando custos e insumos. Al??m disso, evidenciou-se como sendo mais eficaz o agendamento multiprofissional, no qual o m??dico diz o protocolo que vai ser utilizado, o tempo que vai ser utilizado, e se o paciente tem condi????es de utilizar, mas com o que se refere ao gerenciamento dos recursos concorrentes como leitos, intera????o, poltrona, quimioterapia ou sala cir??rgica, sendo de responsabilidade de um profissional com uma vis??o log??stica do todo, sendo, neste caso, um enfermeiro ou farmac??utico

DNA sequence accounts for the majority of disease heritability, including cancer. Yet, not all familial cancer cases can be explained by genetic factors. It is becoming clear that environmentally induced epigenetic inheritance occurs and that the progeny's traits can be shaped by parental environmental experiences. In humans, epidemiological studies have implicated environmental toxicants, such as the pesticide DDT, in intergenerational cancer development, including breast and childhood tumors. Here, we show that the female progeny of males exposed to DDT in the pre-conception period have higher susceptibility to developing aggressive tumors in mouse models of breast cancer. Sperm of DDT-exposed males exhibited distinct patterns of small non-coding RNAs, with an increase in miRNAs and a specific surge in miRNA-10b levels. Remarkably, embryonic injection of the entire sperm RNA load of DDT-exposed males, or synthetic miRNA-10b, recapitulated the tumor phenotypes observed in DDT offspring. Mechanistically, miR-10b injection altered the transcriptional profile in early embryos with enrichment of genes associated with cell differentiation, tissue and immune system development. In adult DDT-derived progeny, transcriptional and protein analysis of mammary tumors revealed alterations in stromal and in immune system compartments. Our findings reveal a causal role for sperm RNAs in environmentally induced inheritance of cancer predisposition and, if confirmed in humans, this could help partially explain some of the "missing heritability" of breast, and other, malignancies., Competing Interests: The authors declare they have no actual or potential competing financial interests