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Your search keyword '"Bible, Amber N."' showing total 25 results
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25 results on '"Bible, Amber N."'

13. Integrated Proteomics and Lipidomics Reveal That the Swarming Motility of Paenibacillus polymyxa Is Characterized by Phospholipid Modification, Surfactant Deployment, and Flagellar Specialization Relative to Swimming Motility

Author

Poudel, Suresh, primary, Giannone, Richard J., additional, Farmer, Abigail T., additional, Campagna, Shawn R., additional, Bible, Amber N., additional, Morrell-Falvey, Jennifer L., additional, Elkins, James G., additional, and Hettich, Robert L., additional

Published
2019
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18. Stochastic Assembly of Bacteria in Microwell Arrays Reveals the Importance of Confinement in Community Development

Author

Hansen, Ryan H., Timm, Collin M., Timm, Andrea C., Bible, Amber N., and Morrell-Falvey, Jennifer L.

Abstract

The structure and function of microbial communities is deeply influenced by the physical and chemical architecture of the local microenvironment and the abundance of its community members. The complexity of this natural parameter space has made characterization of the key drivers of community development difficult. In order to facilitate these characterizations, we have developed a microwell platform designed to screen microbial growth and interactions across a wide variety of physical and initial conditions. Assembly of microbial communities into microwells was achieved using a novel biofabrication method that exploits well feature sizes for control of innoculum levels. Wells with incrementally smaller size features created populations with increasingly larger variations in inoculum levels. This allowed for reproducible growth measurement in large wells, and screening for favorable growth conditions in small wells. We demonstrate the utility of this approach for screening and discovery using 5 ë_m wells to assemble P. aeruginosa colonies across a broad distribution of innoculum levels, and identify those conditions that promote the highest probability of survivial and growth under spatial confinement. Multi-member community assembly was also characterized to demonstrate the broad potential of this platform for studying the role of member abundance on microbial competition, mutualism and community succession.

Published
2016

21. A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization

Author

Bible, Amber N., primary, Fletcher, Sarah J., additional, Pelletier, Dale A., additional, Schadt, Christopher W., additional, Jawdy, Sara S., additional, Weston, David J., additional, Engle, Nancy L., additional, Tschaplinski, Timothy, additional, Masyuko, Rachel, additional, Polisetti, Sneha, additional, Bohn, Paul W., additional, Coutinho, Teresa A., additional, Doktycz, Mitchel J., additional, and Morrell-Falvey, Jennifer L., additional

Published
2016
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25. Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoeasp. YR343

Author

Estenson, Kasey, Hurst, Gregory B., Standaert, Robert F., Bible, Amber N., Garcia, David, Chourey, Karuna, Doktycz, Mitchel J., and Morrell-Falvey, Jennifer L.

Abstract

Indole-3-acetic acid (IAA) plays a central role in plant growth and development, and many plant-associated microbes produce IAA using tryptophan as the precursor. Using genomic analyses, we predicted that Pantoeasp. YR343, a microbe isolated from Populus deltoides, synthesizes IAA using the indole-3-pyruvate (IPA) pathway. To better understand IAA biosynthesis and the effects of IAA exposure on cell physiology, we characterized proteomes of Pantoeasp. YR343 grown in the presence of tryptophan or IAA. Exposure to IAA resulted in upregulation of proteins predicted to function in carbohydrate and amino acid transport and exopolysaccharide (EPS) biosynthesis. Metabolite profiles of wild-type cells showed the production of IPA, IAA, and tryptophol, consistent with an active IPA pathway. Finally, we constructed an ΔipdCmutant that showed the elimination of tryptophol, consistent with a loss of IpdC activity, but was still able to produce IAA (20% of wild-type levels). Although we failed to detect intermediates from other known IAA biosynthetic pathways, this result suggests the possibility of an alternate pathway or the production of IAA by a nonenzymatic route in Pantoeasp. YR343. The ΔipdCmutant was able to efficiently colonize poplar, suggesting that an active IPA pathway is not required for plant association.

Published
2018
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