Search

Your search keyword '"Boxall, Sally"' showing total 18 results
Start Over Author "Boxall, Sally" Publication Year Range Last 10 years
18 results on '"Boxall, Sally"'

5. Antenatal counselling for prospective parents whose fetus has a neurological anomaly: part 1, experiences and recommendations for service design

Author

Hart, Anthony R., Vollmer, Brigitte, Howe, David, Boxall, Sally, Foulds, Nicola, De Lacey, Patricia, Vasudevan, Chakra, Griffiths, Paul, and Piercy, Hilary

Abstract

Prospective parents whose fetus is diagnosed with a neurological anomaly go through a complex range of emotions. They describe their discussions of antenatal counselling from health care professionals as focusing too much on the nature of the anomaly involving unintelligible medical terminology, when what they really want is a picture of the best- and worst-case scenarios. Whilst information on the level of risk for their fetus is important, it is not the parents’ primary concern. When statistics for risk are given, they may not be as well understood as the health care professionals think. This review discusses the published evidence on antenatal counselling and recommendations for explaining risk to parents of fetuses with neurological anomalies. From this data we make recommendations for the organization of antenatal counselling services.

Published
2022

9. Upregulated WEE1 protects endothelial cells of colorectal cancer liver metastases

Author

Webster, Peter J., Littlejohns, Anna T., Gaunt, Hannah J., Young, Richard S., Rode, Baptiste, Ritchie, Judith E., Stead, Lucy F., Harrison, Sally, Droop, Alastair, Martin, Heather L., Tomlinson, Darren C., Hyman, Adam J., Appleby, Hollie L., Boxall, Sally, Bruns, Alexander F., Li, Jing, Prasad, Raj K., Lodge, J. Peter A., Burke, Dermot A., and Beech, David J.

Subjects
Neovascularization, Pathologic, Caspase 3, Liver Neoplasms, Endothelial Cells, Nuclear Proteins, colorectal cancer, Apoptosis, Cell Cycle Proteins, Protein-Tyrosine Kinases, Platelet Endothelial Cell Adhesion Molecule-1, angiogenesis, checkpoint kinase, endothelial cell, DNA damage, Humans, DNA Breaks, Double-Stranded, Colorectal Neoplasms, Research Paper
Abstract

Surgical resection of colorectal cancer liver metastases (CLM) can be curative, yet 80% of patients are unsuitable for this treatment. As angiogenesis is a determinant of CLM progression we isolated endothelial cells from CLM and sought a mechanism which is upregulated, essential for angiogenic properties of these cells and relevant to emerging therapeutic options. Matched CLM endothelial cells (CLMECs) and endothelial cells of normal adjacent liver (LiECs) were superficially similar but transcriptome sequencing revealed molecular differences, one of which was unexpected upregulation and functional significance of the checkpoint kinase WEE1. Western blotting confirmed that WEE1 protein was upregulated in CLMECs. Knockdown of WEE1 by targeted short interfering RNA or the WEE1 inhibitor AZD1775 suppressed proliferation and migration of CLMECs. Investigation of the underlying mechanism suggested induction of double-stranded DNA breaks due to nucleotide shortage which then led to caspase 3-dependent apoptosis. The implication for CLMEC tube formation was striking with AZD1775 inhibiting tube branch points by 83%. WEE1 inhibitors might therefore be a therapeutic option for CLM and could be considered more broadly as anti-angiogenic agents in cancer treatment.

Published
2017

10. Antenatal counselling for prospective parents whose fetus has a neurological anomaly: part 2, risks of adverse outcome in common anomalies.

Author

Hart, Anthony R, Vasudevan, Chakra, Griffiths, Paul D, Foulds, Nicola, Piercy, Hilary, de Lacy, Patricia, Boxall, Sally, Howe, David, and Vollmer, Brigitte

Subjects
PARENTS, AGENESIS of corpus callosum, COUNSELING, MEDICAL personnel, FETAL abnormalities
Abstract

After diagnosis of a fetal neurological anomaly, prospective parents want to know the best and worst‐case scenarios and an estimation of the risk to their infant of having an atypical developmental outcome. The literature on developmental outcomes for fetal neurological anomalies is poor: studies are characterized by retrospective design, small sample size, often no standardized assessment of development, and differing definitions of anomalies. This review provides an aide‐memoir on the risks of adverse neurodevelopmental outcome for ventriculomegaly, cortical anomalies, microcephaly, macrocephaly, agenesis of the corpus callosum, posterior fossa anomalies, and myelomeningocele, to assist healthcare professionals in counselling. The data in this review should be used alongside recommendations on counselling and service design described in part 1 to provide antenatal counselling. This invited review is commented by Ferrand and Racine on page 6 of this issue. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

11. Antenatal counselling for prospective parents whose fetus has a neurological anomaly: part 1, experiences and recommendations for service design.

Author

Hart, Anthony R, Vollmer, Brigitte, Howe, David, Boxall, Sally, Foulds, Nicola, de Lacy, Patricia, Vasudevan, Chakra, Griffiths, Paul D, and Piercy, Hilary

Subjects
MEDICAL personnel, SERVICE design, QUALITY of service, COUNSELING, FETUS, MEDICAL terminology
Abstract

Prospective parents whose fetus is diagnosed with a neurological anomaly go through a complex range of emotions. They describe their discussions of antenatal counselling from health care professionals as focusing too much on the nature of the anomaly involving unintelligible medical terminology, when what they really want is a picture of the best- and worst-case scenarios. Whilst information on the level of risk for their fetus is important, it is not the parents' primary concern. When statistics for risk are given, they may not be as well understood as the health care professionals think. This review discusses the published evidence on antenatal counselling and recommendations for explaining risk to parents of fetuses with neurological anomalies. From this data we make recommendations for the organization of antenatal counselling services. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

18. The use of multiparameter flow cytometry and cell sorting to characterize native human bone marrow mesenchymal stem cells (MSC).

Author

Boxall S and Jones E

Subjects
Adapalene, Cell Separation methods, Humans, Leukocyte Common Antigens analysis, Mesenchymal Stem Cells metabolism, Naphthalenes analysis, Phenotype, Flow Cytometry methods, Mesenchymal Stem Cells cytology
Abstract

This chapter describes a method for identification, phenotypic analysis, and cell sorting of rare mesenchymal stem cells (MSCs) from human bone marrow (BM) aspirates. The native BM MSC population is identified based on the CD45(-/low)CD271(+) phenotype. The method consists of three related procedures: Procedure 1 involves a microbead-based pre-enrichment step. Two other procedures describe direct flow cytometric analysis of MSCs following the isolation of the mononuclear cell (MNC) fraction (Procedure 2) or more rapidly, following a simple ammonium chloride-based red cell lysis (Procedure 3). Recently described multi-lineage transcript expression in the CD45(-/low)CD271(+) cells suggests that the native BM MSC fraction could be further subdivided into functionally distinct subpopulations. The present protocols are hoped to help MSC biologists to enter this exciting field of research and to take it forward towards a better understanding of MSC biology in vivo.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results