28 results on '"Campos, Fabrício S."'
Search Results
2. Prediction of fermentative parameters from mathematical modeling using thermotolerant probiotic yeast
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Cangussu, Alex Sander R., Cruz, Pedro A., Souza Aguiar, Raimundo W., Santos, Gil R., Campos, Fabricio S., Sobrinho, Eliane M., Brandi, Igor V., Viana, Kelvinson F., Nogueira, Paulo A., Passos, Frederico J.V., Moraes, Celia A., Prazeres, Gilzeane S.S., da Silveira, Wendel B., and Passos, Flávia Maria Lopes
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- 2020
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3. Evaluation of the anti-apoptotic activity of bovine alphaherpesvirus type 5 US3 protein kinase in insect cells using a recombinant baculovirus
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Silva, Alice M., Morgado, Fabrício S., Silva, Leonardo A., Borges, José R. J., Perecmanis, Simone, Ardisson-Araújo, Daniel M. P., Ribeiro, Bergmann M., and Campos, Fabrício S.
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- 2020
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4. Yellow Fever Virus Maintained by Sabethes Mosquitoes during the Dry Season in Cerrado, a Semiarid Region of Brazil, in 2021
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Oliveira, Cirilo H. de, primary, Andrade, Miguel S., additional, Campos, Fabrício S., additional, Cardoso, Jader da C., additional, Gonçalves-dos-Santos, Maria Eduarda, additional, Oliveira, Ramon Silva, additional, Aquino-Teixeira, Sandy Micaele, additional, Campos, Aline AS, additional, Almeida, Marco AB, additional, Simonini-Teixeira, Danilo, additional, Sevá, Anaiá da P., additional, Temponi, Andrea Oliveira Dias, additional, Magalhães, Fernando Maria, additional, Menezes, Agna Soares da Silva, additional, Lopes, Bartolomeu Teixeira, additional, Almeida, Hermes P., additional, Pedroso, Ana Lúcia, additional, Gonçalves, Giovani Pontel, additional, Chaves, Danielle Costa Capistrano, additional, Menezes, Givaldo Gomes de, additional, Bernal-Valle, Sofía, additional, Müller, Nicolas FD, additional, Janssen, Luis, additional, Santos, Edmilson dos, additional, Mares-Guia, Maria A., additional, Albuquerque, George R., additional, Romano, Alessandro PM, additional, Franco, Ana C., additional, Ribeiro, Bergmann M., additional, Roehe, Paulo M., additional, Lourenço-de-Oliveira, Ricardo, additional, and Abreu, Filipe Vieira Santos de, additional
- Published
- 2023
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5. Evaluation of the anti-apoptotic activity of bovine alphaherpesvirus type 5 US3 protein kinase in insect cells using a recombinant baculovirus
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Silva, Alice M., Morgado, Fabrício S., Silva, Leonardo A., Borges, José R. J., Perecmanis, Simone, Ardisson-Araújo, Daniel M. P., Ribeiro, Bergmann M., and Campos, Fabrício S.
- Abstract
Bovine alphaherpesvirustype 5 (BoHV-5) is one of the main agents responsible for meningoencephalitis in cattle in Brazil, causing significant economic losses. It is known that other viruses of the Herpesviridaefamily such as Bovine alphaherpesvirustype 1, Swine alphaherpesvirustype 1, and the Human alphaherpesvirustypes 1 and 2 encode genes homologous to BoHV-5, with recognized action in the control of apoptosis. The objective of this work was to express the BoHV-5 US3 gene in a baculovirus-based expression system for the production of the serine/threonine kinase protein and to evaluate its activity in the control of apoptosis in vitro. A recombinant baculovirus derived from the Autographa californica multiple nucleopolyhedrovirus(AcMNPV) containing the US3 gene and a deletion in the baculovirus anti-apoptotic p35gene was constructed using the Bac-to-Bac™ system. This recombinant baculovirus was used to evaluate the anti-apoptotic activity of the recombinant US3 protein in insect cells comparing with two other AcMNPV recombinants, one containing a functional copy of the AcMNPV anti-apoptotic p35gene and an AcMNPV p35knockout virus with the anti-apoptotic iap-3gene from Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV). We found that the caspase level was higher in insect cells infected with the US3-contanining recombinant virus than in cells infected with the AcMNPV recombinants containing the p35and iap-3genes. These results indicate that the BoHV-5 US3 protein kinase gene is not able to block apoptosis in insect cells induced by the infection of a p35knockout AcMNPV.
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- 2024
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6. Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains
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Alves, Giselly B., Melo, Fernando L., Oliveira, Eugenio E., Haddi, Khalid, Costa, Lara T. M., Dias, Marcelo L., Campos, Fabrício S., Pereira, Eliseu J. G., Corrêa, Roberto F. T., Ascêncio, Sergio D., Santos, Gil R., Smagghe, Guy, Ribeiro, Bergmann M., and Aguiar, Raimundo W. S.
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- 2020
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7. Yellow Fever Virus Maintained by Sabethes Mosquitoes during the Dry Season in Cerrado, a Semiarid Region of Brazil, in 2021.
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de Oliveira, Cirilo H., Andrade, Miguel S., Campos, Fabrício S., da C. Cardoso, Jader, Gonçalves-dos-Santos, Maria Eduarda, Oliveira, Ramon Silva, Aquino-Teixeira, Sandy Micaele, Campos, Aline AS, Almeida, Marco AB, Simonini-Teixeira, Danilo, da P. Sevá, Anaiá, Temponi, Andrea Oliveira Dias, Magalhães, Fernando Maria, da Silva Menezes, Agna Soares, Lopes, Bartolomeu Teixeira, Almeida, Hermes P., Pedroso, Ana Lúcia, Gonçalves, Giovani Pontel, Chaves, Danielle Costa Capistrano, and de Menezes, Givaldo Gomes
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YELLOW fever ,CERRADOS ,PHYTOPLASMAS ,MOSQUITOES ,WEATHER ,PHYLOGEOGRAPHY ,ARID regions - Abstract
In recent decades, waves of yellow fever virus (YFV) from the Amazon Rainforest have spread and caused outbreaks in other regions of Brazil, including the Cerrado, a savannah-like biome through which YFV usually moves before arriving at the Atlantic Forest. To identify the vectors involved in the maintenance of the virus in semiarid environments, an entomological survey was conducted after confirmation of yellow fever (YF) epizootics at the peak of the dry season in the Cerrado areas of the state of Minas Gerais. In total, 917 mosquitoes from 13 taxa were collected and tested for the presence of YFV. Interestingly, mosquitoes of the Sabethes genus represented 95% of the diurnal captured specimens, displaying a peak of biting activity never previously recorded, between 4:30 and 5:30 p.m. Molecular analysis identified three YFV-positive pools, two from Sabethes chloropterus—from which near-complete genomes were generated—and one from Sa. albiprivus, whose low viral load prevented sequencing. Sa. chloropterus was considered the primary vector due to the high number of copies of YFV RNA and the high relative abundance detected. Its bionomic characteristics allow its survival in dry places and dry time periods. For the first time in Brazil, Sa. albiprivus was found to be naturally infected with YFV and may have played a role as a secondary vector. Despite its high relative abundance, fewer copies of viral RNA were found, as well as a lower Minimum Infection Rate (MIR). Genomic and phylogeographic analysis showed that the virus clustered in the sub-lineage YFV
PA-MG , which circulated in Pará in 2017 and then spread into other regions of the country. The results reported here contribute to the understanding of the epidemiology and mechanisms of YFV dispersion and maintenance, especially in adverse weather conditions. The intense viral circulation, even outside the seasonal period, increases the importance of surveillance and YFV vaccination to protect human populations in affected areas. [ABSTRACT FROM AUTHOR]- Published
- 2023
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8. Complete Genome Sequences of Two Bovine Alphaherpesvirus 5 Subtype C Strains from Southeast Brazil
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Paim, Willian P., primary, Campos, Fabrício S., additional, Cibulski, Samuel P., additional, Scheffer, Camila M., additional, Tochetto, Caroline, additional, Varela, Ana P. M., additional, Junqueira, Dennis M., additional, Mayer, Fabiana Q., additional, Romijn, Phyllis C., additional, Pituco, Edviges M., additional, Franco, Ana C., additional, Spilki, Fernando R., additional, and Roehe, Paulo M., additional
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- 2022
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9. Fast surveillance response and genome sequencing reveal the circulation of a new Yellow Fever Virus sublineage in 2021, in Minas Gerais, Brazil
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Andrade, Miguel S., primary, Campos, Fabrício S., additional, de Oliveira, Cirilo H., additional, Oliveira, Ramon Silva, additional, Campos, Aline A. S., additional, Almeida, Marco A. B., additional, Simonini-Teixeira, Danilo, additional, Sevá, Anaiá da P., additional, Temponi, Andrea Oliveira Dias, additional, Magalhães, Fernando Maria, additional, Chaves, Danielle Costa Capistrano, additional, Pereira, Maira Alves, additional, Lamounier, Ludmila Oliveira, additional, de Menezes, Givaldo Gomes, additional, Teixeira, Sandy Micaele Aquino, additional, dos Santos, Maria Eduarda Gonçalves, additional, Bernal-Valle, Sofía, additional, Müller, Nicolas F. D., additional, Cardoso, Jader da C., additional, dos Santos, Edmilson, additional, Mares-Guia, Maria A., additional, Albuquerque, George R., additional, Romano, Alessandro P. M., additional, Franco, Ana C., additional, Ribeiro, Bergmann M., additional, Roehe, Paulo M., additional, and Abreu, Filipe V. S., additional
- Published
- 2021
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10. Identification of potential new mosquito-associated viruses of adult Aedes aegypti mosquitoes from Tocantins state, Brazil
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Duarte, Matheus A., primary, Campos, Fabrício S., additional, Araújo Neto, Osvaldo F., additional, Silva, Leonardo A., additional, Silva, Arthur B., additional, Aguiar, Thalita C., additional, Santos, Raissa N., additional, Souza, Ueric J. B., additional, Alves, Giselly B., additional, Melo, Fernando L., additional, Ardisson-Araujo, Daniel M. P., additional, Aguiar, Raimundo W. S., additional, and Ribeiro, Bergmann M., additional
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- 2021
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11. Real-Time Genomic Surveillance during the 2021 Re-Emergence of the Yellow Fever Virus in Rio Grande do Sul State, Brazil
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Andrade, Miguel de S., primary, Campos, Fabrício S., additional, Campos, Aline A. S., additional, Abreu, Filipe V. S., additional, Melo, Fernando L., additional, Sevá, Anaiá da P., additional, Cardoso, Jader da C., additional, Dos Santos, Edmilson, additional, Born, Lucas C., additional, Silva, Cláudia M. D. da, additional, Müller, Nicolas F. D., additional, Oliveira, Cirilo H. de, additional, Silva, Alex J. J. da, additional, Simonini-Teixeira, Danilo, additional, Bernal-Valle, Sofía, additional, Mares-Guia, Maria A. M. M., additional, Albuquerque, George R., additional, Romano, Alessandro P. M., additional, Franco, Ana C., additional, Ribeiro, Bergmann M., additional, Roehe, Paulo M., additional, and Almeida, Marco A. B. de, additional
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- 2021
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12. Antibacterial activity of Siparuna guianensis essential oil mediated by impairment of membrane permeability and replication of pathogenic bacteria
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de Souza Moura, Wellington, primary, de Souza, Silvania Rosa, additional, Campos, Fabrício S., additional, Sander Rodrigues Cangussu, Alex, additional, Macedo Sobrinho Santos, Eliane, additional, Silva Andrade, Bruno, additional, Borges Gomes, Cesar Henrique, additional, Fernandes Viana, Kelvinson, additional, Haddi, Khalid, additional, Oliveira, Eugenio Eduardo, additional, Nascimento, Vitor L., additional, and de Souza Aguiar, Raimundo Wagner, additional
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- 2020
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13. Complete Sequences of Two Plasmids Found in a Brazilian Bacillus thuringiensis Serovar israelensis Strain
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Campos, Fabrício S., primary, Cerqueira, Fernando B., additional, Santos, Gil R., additional, Pereira, Eliseu J. G., additional, Corrêia, Roberto F. T., additional, Cangussu, Alex S. R., additional, Melo, Fernando L., additional, Ribeiro, Bergmann M., additional, and Aguiar, Raimundo W. S., additional
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- 2019
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14. Genome Sequence of a New Siphoviridae Phage Found in a Brazilian Bacillus thuringiensis Serovar israelensis Strain
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Campos, Fabrício S., primary, Santos, Gil R., additional, Nascimento, Vitor L., additional, Corrêia, Roberto F. T., additional, Cangussu, Alex S. R., additional, Hoffmann, Karen, additional, Oliveira, Eugênio E., additional, Ribeiro, Bergmann M., additional, and Aguiar, Raimundo W. S., additional
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- 2018
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15. Genome Characterization of the First Mimiviruses of Lineage C Isolated in Brazil
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Assis, Felipe L., primary, Franco-Luiz, Ana P. M., additional, Santos, Raíssa N. dos, additional, Campos, Fabrício S., additional, Dornas, Fábio P., additional, Borato, Paulo V. M., additional, Franco, Ana C., additional, Abrahao, Jônatas S., additional, Colson, Philippe, additional, and Scola, Bernard La, additional
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- 2017
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16. Identification of potential new mosquito-associated viruses of adult Aedes aegyptimosquitoes from Tocantins state, Brazil
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Duarte, Matheus A., Campos, Fabrício S., Araújo Neto, Osvaldo F., Silva, Leonardo A., Silva, Arthur B., Aguiar, Thalita C., Santos, Raissa N., Souza, Ueric J. B., Alves, Giselly B., Melo, Fernando L., Ardisson-Araujo, Daniel M. P., Aguiar, Raimundo W. S., and Ribeiro, Bergmann M.
- Abstract
Medically important arboviruses such as dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) are primarily transmitted by the globally distributed mosquito Aedes aegypti. Increasing evidence suggests that the transmission of some viruses can be influenced by mosquito-specific and mosquito-borne viruses. Advancements in high-throughput sequencing (HTS) and bioinformatics have expanded our knowledge on the richness of viruses harbored by mosquitoes. HTS was used to characterize the presence of virus sequences in wild-caught adult Ae.aegyptifrom Tocantins (TO) state, Brazil. Samples of mosquitoes were collected in four cities of Tocantins state and submitted to RNA isolation, followed by sequencing at an Illumina HiSeq platform. Our results showed initially by Krona the presence of 3% of the sequenced reads belonging to the viral database. After further analysis, the virus sequences were found to have homology to two viral families found in insects Phenuiviridaeand Metaviridae. Three possible viral strains including putative new viruses were detected and named Phasi Charoen-like phasivirus isolate To-1 (PCLV To-1), Aedesaegypti To virus 1 (AAToV1), and Aedesaegypti To virus 2 (AAToV2). The results presented in this work contribute to the growing knowledge about the diversity of viruses in mosquitoes and might be useful for future studies on the interaction between insect-specific viruses and arboviruses.
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- 2021
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17. Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle
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Siedler, Bianca S., primary, Roloff, Bárbara C., additional, de Sá, Gizele L., additional, Neis, Alessandra, additional, Conceição, Fabrício R., additional, Hartwig, Daiane D., additional, Borsuk, Sibele, additional, Dellagostin, Odir A., additional, Campos, Fabrício S., additional, Roehe, Paulo M., additional, Hartleben, Claudia P., additional, and McBride, Alan J.A., additional
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- 2017
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18. Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5
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Dummer, Luana A., primary, Araujo, Itauá L., additional, Campos, Fabrício S., additional, da Rosa, Matheus C., additional, Finger, Paula F., additional, de Oliveira, Patricia D., additional, Conceição, Fabricio R., additional, Fischer, Geferson, additional, Roehe, Paulo M., additional, and Leite, Fábio P. L., additional
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- 2016
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19. Within-Host and Between-Host Evolution in SARS-CoV-2—New Variant's Source.
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Moelling, Karin, Campos, Fabrício S., Arruda, Luciana Barros de, and Vaslin, Maite F.S.
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MEDICAL personnel , *SARS-CoV-2 , *IMMUNOCOMPROMISED patients , *SYNTHETIC antibodies , *VIRAL mutation - Abstract
Some of the newly emerging corona viral variants show high numbers of mutations. This is unexpected for a virus with a low mutation rate due to an inherent proof-reading system. Could such a variant arise under very special conditions occurring in a host where the virus replicates and mutates in a rather unlimited fashion, such as in immune compromised patients? The virus was shown to replicate in an immunosuppressed cancer patient for more than 105 days and might be a source of new variants. These patients are asymptomatic and the virus may therefore escape detection and attention and be high-risk. Similarly, HIV-infected individuals may be immunocompromised and support coronavirus replication with increased mutation rates. The patients may promote "within-host evolution". Some of the viruses present in such a highly mutagenic swarm or quasispecies within one patient may become founders and cause a pandemic by further "between-host evolution". B.1.1.7 with 23 mutations may be such a case. Immunosuppressed patients can be identified and treated by the synthetic antibody cocktails as passive immunization and kept under control. Immunosuppressed patients can be easily identified and supervised by healthcare workers—once they become aware of the risk—to avoid new variants with pandemic potential. [ABSTRACT FROM AUTHOR]
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- 2021
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20. Hepatitis E Virus in People Who Use Crack-Cocaine: A Cross-Sectional Study in a Remote Region of Northern Brazil.
- Author
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do Nascimento, Raquel Silva, Baia, Karen Lorena N., de Souza, Samara Borges, Fontoura, Guilherme Martins G., Nunes, Patrícia Ferreira, Machado, Luiz Fernando A., Kupek, Emil, Fischer, Benedikt, Martins, Luísa Caricio, Oliveira-Filho, Aldemir B., de Arruda, Luciana Barros, Campos, Fabrício S., and da Fonseca, Flavio Guimaraes
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SHARED housing ,CROSS-sectional method ,HEPATITIS E virus ,DIAGNOSIS ,SOCIAL groups ,HOMELESSNESS ,SOCIAL services ,BLOOD sampling - Abstract
People who use crack-cocaine (PWUCC) have numerous vulnerabilities and pose a challenge to health and social assistance services. The exposure to pathogens and risk situations occur differently according to each individual, region and social group. This study identified the presence, genotypes and factors associated with hepatitis E virus (HEV) exposure among a community-recruited cohort of 437 PWUCC in northern Brazil. Epidemiological information was collected through community-based assessments and interviews. Thereafter, blood and fecal samples were collected and tested for HEV using an immunoenzymatic assay, and the genotype was identified by PCR. Logistic regressions were used to identify the risk factors independently associated with exposure to HEV. In total, 79 (18.1%) PWUCC were exposed to HEV: 73 (16.7%) for IgG and six for IgG + IgM. HEV RNA was detected in six fecal samples and in two blood samples from PWUCC with IgM + IgG. Subtype 3c was identified in all of the samples. The factors associated with exposure to HEV were low monthly income, unstable housing (e.g., homelessness), crack-cocaine use ≥40 months, and the shared use of crack-cocaine equipment. The current study provides unique initial insights into HEV status and risk factors among PWUCC in a remote area in Brazil, with diverse implications for urgently improved diagnosis, prevention, and treatment intervention needs. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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21. The Epidemiological Impact of STIs among General and Vulnerable Populations of the Amazon Region of Brazil: 30 years of Surveillance.
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Machado, Luiz Fernando Almeida, Fonseca, Ricardo Roberto de Souza, Queiroz, Maria Alice Freitas, Oliveira-Filho, Aldemir Branco, Cayres-Vallinoto, Izaura Maria Vieira, Vallinoto, Antonio Carlos Rosário, Ishak, Marluísa de Oliveira Guimarães, Ishak, Ricardo, de Arruda, Luciana Barros, Campos, Fabrício S., and da Fonseca, Flavio Guimaraes
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DRUG abuse ,CITY dwellers ,HUMAN Development Index ,MOLECULAR epidemiology ,CHLAMYDIA trachomatis ,TREPONEMA pallidum ,SEXUALLY transmitted diseases - Abstract
Sexually transmitted infections (STIs) represent a worldwide public health problem and, although many of them are curable, they continue to be neglected, especially in areas with a low human development index, such as in the northern region of Brazil. This review describes the results of 30 years of studies at the Virus Laboratory at the Federal University of Pará, including the prevalence and molecular epidemiology of HIV-1, HTLV-1/2, HPV, HBV, Treponema pallidum and Chlamydia trachomatis among urban and non-urban populations, and also in vulnerable groups in the Brazilian Amazon. Control strategies and challenges in preventing STIs are discussed considering this immense geographic region, where essential health services are unable to reach the entire population, especially the most vulnerable, such as female sex workers, people who use illicit drugs, remnants of quilombolos and indigenous communities. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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22. Genetic Characterization and Pathogenesis of Avian Influenza Virus H7N3 Isolated from Spot-Billed Ducks in South Korea, Early 2019.
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Trinh, Thuy-Tien Thi, Tiwari, Indira, Durairaj, Kaliannan, Duong, Bao Tuan, Nguyen, Anh Thi Viet, Tuong, Hien Thi, Hoang, Vui Thi, Than, Duong Duc, Nam, SunJeong, Yeo, Seon-Ju, Park, Hyun, and Campos, Fabrício S.
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AVIAN influenza A virus ,AVIAN influenza ,BASIC proteins ,GENETIC mutation ,AMINO acid sequence ,DUCK plague - Abstract
Low-pathogenicity avian influenza viruses (LPAIV) introduced by migratory birds circulate in wild birds and can be transmitted to poultry. These viruses can mutate to become highly pathogenic avian influenza viruses causing severe disease and death in poultry. In March 2019, an H7N3 avian influenza virus—A/Spot-billed duck/South Korea/WKU2019-1/2019 (H7N3)—was isolated from spot-billed ducks in South Korea. This study aimed to evaluate the phylogenetic and mutational analysis of this isolate. Molecular analysis revealed that the genes for HA (hemagglutinin) and NA (neuraminidase) of this strain belonged to the Central Asian lineage, whereas genes for other internal proteins such as polymerase basic protein 1 (PB1), PB2, nucleoprotein, polymerase acidic protein, matrix protein, and non-structural protein belonged to that of the Korean lineage. In addition, a monobasic amino acid (PQIEPR/GLF) at the HA cleavage site, and the non-deletion of the stalk region in the NA gene indicated that this isolate was a typical LPAIV. Nucleotide sequence similarity analysis of HA revealed that the highest homology (99.51%) of this isolate is to that of A/common teal/Shanghai/CM1216/2017 (H7N7), and amino acid sequence of NA (99.48%) was closely related to that of A/teal/Egypt/MB-D-487OP/2016 (H7N3). An in vitro propagation of the A/Spot-billed duck/South Korea/WKU2019-1/2019 (H7N3) virus showed highest (7.38 Log
10 TCID50 /mL) virus titer at 60 h post-infection, and in experimental mouse lungs, the virus was detected at six days' post-infection. Our study characterizes genetic mutations, as well as pathogenesis in both in vitro and in vivo model of a new Korea H7N3 viruses in 2019, carrying multiple potential mutations to become highly pathogenic and develop an ability to infect humans; thus, emphasizing the need for routine surveillance of avian influenza viruses in wild birds. [ABSTRACT FROM AUTHOR]- Published
- 2021
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23. Global Discrepancies between Numbers of Available SARS-CoV-2 Genomes and Human Development Indexes at Country Scales.
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Colson, Philippe, Raoult, Didier, Vaslin, Maite F.S., Campos, Fabrício S., and de Arruda, Luciana Barros
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HUMAN Development Index ,SARS-CoV-2 ,GENOMES ,WESTERN countries ,GROSS domestic product ,COMPARATIVE genomics ,LIFE expectancy - Abstract
It has now been over a year since SARS-CoV-2 first emerged in China, in December 2019, and it has spread rapidly around the world. Some variants are currently considered of great concern. We aimed to analyze the numbers of SARS-CoV-2 genome sequences obtained in different countries worldwide until January 2021. On 28 January 2021, we downloaded the deposited genome sequence origin from the GISAID database, and from the "Our world in data" website we downloaded numbers of SARS-CoV-2-diagnosed cases, numbers of SARS-CoV-2-associated deaths, population size, life expectancy, gross domestic product (GDP) per capita, and human development index per country. Files were merged and data were analyzed using Microsoft Excel software. A total of 450,968 SARS-CoV-2 genomes originating from 135 countries on the 5 continents were available. When considering the 19 countries for which the number of genomes per 100 deaths was >100, six were in Europe, while eight were in Asia, three were in Oceania and two were in Africa. Six (30%) of these countries are beyond rank 75, regarding the human development index and four (20%) are beyond rank 80 regarding GDP per capita. Moreover, the comparisons of the number of genomes sequenced per 100 deaths to the human development index by country show that some Western European countries have released similar or lower numbers of genomes than many African or Asian countries with a lower human development index. Previous data highlight great discrepancies between the numbers of available SARS-CoV-2 genomes per 100 cases and deaths and the ranking of countries regarding wealth and development. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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24. Multiple Early Introductions of SARS-CoV-2 to Cape Town, South Africa.
- Author
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Engelbrecht, Susan, Delaney, Kayla, Kleinhans, Bronwyn, Wilkinson, Eduan, Tegally, Houriiyah, Stander, Tania, van Zyl, Gert, Preiser, Wolfgang, de Oliveira, Tulio, de Arruda, Luciana Barros, Campos, Fabrício S., and da Fonseca, Flavio Guimaraes
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COVID-19 ,SARS-CoV-2 ,PANDEMICS ,MAXIMUM likelihood statistics ,MOLECULAR clock - Abstract
Cape Town was the first city in South Africa to experience the full impact of the coronavirus disease 2019 (COVID-19) pandemic. We acquired samples from all suspected cases and their contacts during the first month of the pandemic from Tygerberg Hospital. Nanopore sequencing generated SARS-CoV-2 whole genomes. Phylogenetic inference with maximum likelihood and Bayesian methods were used to determine lineages that seeded the local epidemic. Three patients were known to have travelled internationally and an outbreak was detected in a nearby supermarket. Sequencing of 50 samples produced 46 high-quality genomes. The sequences were classified as lineages: B, B.1, B.1.1.1, B.1.1.161, B.1.1.29, B.1.8, B.39, and B.40. All the sequences from persons under investigation (PUIs) in the supermarket outbreak (lineage B.1.8) fall within a clade from the Netherlands with good support (p > 0.9). In addition, a new mutation, 5209A>G, emerged within the Cape Town cluster. The molecular clock analysis suggests that this occurred around 13 March 2020 (95% confidence interval: 9–17 March). The phylogenetic reconstruction suggests at least nine early introductions of SARS-CoV-2 into Cape Town and an early localized transmission in a shopping environment. Genomic surveillance was successfully used to investigate and track the spread of early introductions of SARS-CoV-2 in Cape Town. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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25. In Vitro and In Vivo Models for Studying SARS-CoV-2, the Etiological Agent Responsible for COVID-19 Pandemic.
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Rosa, Rafael B., Dantas, Willyenne M., do Nascimento, Jessica C. F., da Silva, Murilo V., de Oliveira, Ronaldo N., Pena, Lindomar J., de Arruda, Luciana Barros, Campos, Fabrício S., and da Fonseca, Flavio Guimaraes
- Subjects
COVID-19 pandemic ,SARS-CoV-2 ,HAMSTERS ,COVID-19 ,PANDEMICS ,CELL culture - Abstract
The emergence and rapid worldwide spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has prompted the scientific community to rapidly develop in vitro and in vivo models that could be applied in COVID-19 research. In vitro models include two-dimensional (2D) cultures of immortalized cell lines or primary cells and three-dimensional (3D) cultures derived from lung, alveoli, bronchi, and other organs. Although cell-based systems are economic and allow strict control of experimental variables, they do not always resemble physiological conditions. Thus, several in vivo models are being developed, including different strains of mice, hamsters, ferrets, dogs, cats, and non-human primates. In this review, we summarize the main models of SARS-CoV-2 infection developed so far and discuss their advantages, drawbacks and main uses. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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26. Human Respiratory Syncytial Virus Infection in a Human T Cell Line Is Hampered at Multiple Steps.
- Author
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de Souza Cardoso, Ricardo, Viana, Rosa Maria Mendes, Vitti, Brenda Cristina, Coelho, Ana Carolina Lunardello, de Jesus, Bruna Laís Santos, de Paula Souza, Juliano, Pontelli, Marjorie Cornejo, Murakami, Tomoyuki, Ventura, Armando Morais, Ono, Akira, Arruda, Eurico, de Arruda, Luciana Barros, Campos, Fabrício S., and da Fonseca, Flavio Guimaraes
- Subjects
RESPIRATORY syncytial virus infections ,T cells ,CELL lines ,PEDIATRIC respiratory diseases ,FLUORESCENCE in situ hybridization ,GOLGI apparatus ,RESPIRATORY infections - Abstract
Human respiratory syncytial virus (HRSV) is the most frequent cause of severe respiratory disease in children. The main targets of HRSV infection are epithelial cells of the respiratory tract, and the great majority of the studies regarding HRSV infection are done in respiratory cells. Recently, the interest on respiratory virus infection of lymphoid cells has been growing, but details of the interaction of HRSV with lymphoid cells remain unknown. Therefore, this study was done to assess the relationship of HRSV with A3.01 cells, a human CD4
+ T cell line. Using flow cytometry and fluorescent focus assay, we found that A3.01 cells are susceptible but virtually not permissive to HRSV infection. Dequenching experiments revealed that the fusion process of HRSV in A3.01 cells was nearly abolished in comparison to HEp-2 cells, an epithelial cell lineage. Quantification of viral RNA by RT-qPCR showed that the replication of HRSV in A3.01 cells was considerably reduced. Western blot and quantitative flow cytometry analyses demonstrated that the production of HRSV proteins in A3.01 was significantly lower than in HEp-2 cells. Additionally, using fluorescence in situ hybridization, we found that the inclusion body-associated granules (IBAGs) were almost absent in HRSV inclusion bodies in A3.01 cells. We also assessed the intracellular trafficking of HRSV proteins and found that HRSV proteins colocalized partially with the secretory pathway in A3.01 cells, but these HRSV proteins and viral filaments were present only scarcely at the plasma membrane. HRSV infection of A3.01 CD4+ T cells is virtually unproductive as compared to HEp-2 cells, as a result of defects at several steps of the viral cycle: Fusion, genome replication, formation of inclusion bodies, recruitment of cellular proteins, virus assembly, and budding. [ABSTRACT FROM AUTHOR]- Published
- 2021
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27. Markers Associated with COVID-19 Susceptibility, Resistance, and Severity.
- Author
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Fakhroo, Aisha D., Al Thani, Asmaa A., Yassine, Hadi M., de Arruda, Luciana Barros, Campos, Fabrício S., and da Fonseca, Flavio Guimaraes
- Subjects
COVID-19 ,ADULT respiratory distress syndrome ,PANDEMICS ,SARS-CoV-2 ,CYTOKINE release syndrome ,BIOMARKERS - Abstract
In December 2019, the latest member of the coronavirus family, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in Wuhan, China, leading to the outbreak of an unusual viral pneumonia known as coronavirus disease 2019 (COVID-19). COVID-19 was then declared as a pandemic in March 2020 by the World Health Organization (WHO). The initial mortality rate of COVID-19 declared by WHO was 2%; however, this rate has increased to 3.4% as of 3 March 2020. People of all ages can be infected with SARS-CoV-2, but those aged 60 or above and those with underlying medical conditions are more prone to develop severe symptoms that may lead to death. Patients with severe infection usually experience a hyper pro-inflammatory immune reaction (i.e., cytokine storm) causing acute respiratory distress syndrome (ARDS), which has been shown to be the leading cause of death in COVID-19 patients. However, the factors associated with COVID-19 susceptibility, resistance and severity remain poorly understood. In this review, we thoroughly explore the correlation between various host, viral and environmental markers, and SARS-CoV-2 in terms of susceptibility and severity. [ABSTRACT FROM AUTHOR]
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- 2021
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28. Inactivation of Human Coronavirus by Titania Nanoparticle Coatings and UVC Radiation: Throwing Light on SARS-CoV-2.
- Author
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Khaiboullina, Svetlana, Uppal, Timsy, Dhabarde, Nikhil, Subramanian, Vaidyanathan Ravi, Verma, Subhash C., Arruda, Luciana Barros de, Campos, Fabrício S., and Fonseca, Flavio Guimaraes da
- Subjects
SARS-CoV-2 ,COVID-19 ,VIRAL transmission ,PUBLIC spaces ,NANOPARTICLES ,TITANIUM ,ULTRAVIOLET radiation - Abstract
The newly identified pathogenic human coronavirus, SARS-CoV-2, led to an atypical pneumonia-like severe acute respiratory syndrome (SARS) outbreak called coronavirus disease 2019 (abbreviated as COVID-19). Currently, nearly 77 million cases have been confirmed worldwide with the highest numbers of COVID-19 cases in the United States. Individuals are getting vaccinated with recently approved vaccines, which are highly protective in suppressing COVID-19 symptoms but there will be a long way before the majority of individuals get vaccinated. In the meantime, safety precautions and effective disease control strategies appear to be vital for preventing the virus spread in public places. Due to the longevity of the virus on smooth surfaces, photocatalytic properties of "self-disinfecting/cleaning" surfaces appear to be a promising tool to help guide disinfection policies for controlling SARS-CoV-2 spread in high-traffic areas such as hospitals, grocery stores, airports, schools, and stadiums. Here, we explored the photocatalytic properties of nanosized TiO
2 (TNPs) as induced by the UV radiation, towards virus deactivation. Our preliminary results using a close genetic relative of SAR-CoV-2, HCoV-NL63, showed the virucidal efficacy of photoactive TNPs deposited on glass coverslips, as examined by quantitative RT-qPCR and virus infectivity assays. Efforts to extrapolate the underlying concepts described in this study to SARS-CoV-2 are currently underway. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
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