14 results on '"Champagne D"'
Search Results
2. New Insights on Macrophage Function in Pulmonary Hypertension Gained from Comparative Transcriptomics and Metabolomics
- Author
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Li, M., primary, Riddle, S., additional, Kumar, S., additional, D'Alessandro, A., additional, Champagne, D., additional, Zhang, H., additional, Laux, A., additional, McKeon, B.A., additional, Frid, M., additional, Brown, R.D., additional, Hu, C.-J., additional, and Stenmark, K.R., additional
- Published
- 2019
- Full Text
- View/download PDF
3. Updating in working memory predicts greater emotion reactivity to and facilitated recovery from negative emotion-eliciting stimuli
- Author
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Pe, ML, Koval, P, Houben, M, Erbas, Y, Champagne, D, Kuppens, P, Pe, ML, Koval, P, Houben, M, Erbas, Y, Champagne, D, and Kuppens, P
- Abstract
That emotions change in response to emotion-eliciting events is a natural part of human life. However, it is equally important for emotions to return to baseline once the emotion-eliciting events have passed. This suggests that the ability to emotionally react to and recover from emotion-eliciting events is critical for healthy psychological functioning. But why do individuals differ in their emotion reactivity and recovery? The present work postulates that the ability to update emotional information in working memory (WM) may explain individual differences in emotion reactivity and recovery. Two studies are presented, which examined whether updating ability was related to emotion reactivity and recovery. In Study 1, we assessed participants' self-reported affect as they viewed negative and positive films. Our results revealed that better updating ability was related to greater emotion reactivity and facilitated (i.e., quicker) recovery from watching negative films. In Study 2, participants recalled a recent angering event, and were then instructed to either ruminate about or reappraise the event. Results revealed that updating ability was again related to greater emotion reactivity and facilitated (i.e., successful) emotion recovery in response to the angering event, and that this was unrelated to the emotion regulation strategy used. These findings identify the ability to update emotional information in WM as a possible mechanism in emotion responding.
- Published
- 2015
4. comments.
- Author
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Champagne, D., COCKER, GUY, Matlow, R., Hamilton, D., Cummins, M., and Bright, E.
- Subjects
SPARE parts ,MACINTOSH (Computer) ,MICROSOFT software - Abstract
The October issue cover states "Simple two-minute BIOS boosts", but the only mention I could see was on page 25 about updating the BIOS! WE TACKLE TOUGH READER QUESTION ON… > RGB Harmony > BIOS Blues > Time To Upgrade? As Sam said, when setting up your PC, you want to take the time to download the latest version of the BIOS and get it installed to make sure your computer is running at peak performance with the best security. [Extracted from the article]
- Published
- 2022
5. The company landscape for artificial intelligence in large-molecule drug discovery.
- Author
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Nagra NS, van der Veken L, Stanzl E, Champagne D, Devereson A, and Macak M
- Subjects
- Humans, Drug Design, Artificial Intelligence, Drug Discovery
- Published
- 2023
- Full Text
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6. Regional Survey of Diamondback Moth (Lepidoptera: Plutellidae) Response to Maximum Dosages of Insecticides in Georgia and Florida.
- Author
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Riley D, Smith H, Bennett J, Torrance P, Huffman E, Sparks A, Gruver C, Dunn T, and Champagne D
- Subjects
- Animals, Florida, Georgia, Insecticide Resistance, Insecticides pharmacology, Moths
- Abstract
We conducted maximum dose bioassays of insecticide for the control of diamondback moth (DBM), Plutella xylostella (Linnaeus), in cole crops, from 2016 to 2019 at several commercial locations in Georgia and Florida. The nominal maximum dose was defined as the highest labeled rate of an insecticide at the beginning of the survey in the equivalent of 935 liters/ha dilution. The results indicated low insecticide efficacy for high labeled rates of the following insecticides by common name (Insecticide Resistance Action Committee group number in parentheses). Our 4-yr survey identified very low levels of DBM larval control (<47%) by lambda-cyhalothrin (3), methoxyfenozide (18), pyriproxyfen (7C), novaluron (15), bifenthrin (3), chlorantraniliprole (28), indoxacarb (22A), and methomyl (1A). The best products for DBM control (>74%) listed in decreasing average levels of efficacy were naled (1B), cyclaniliprole (28), tolfenpyrad (21A), emamectin benzoate (6), and cyantraniliprole (28). Intermediate levels of control (61-71%) were obtained with Bacillus thuringiensis subspecies aizawai (11A), Bacillus thuringiensis, subsp. kurstaki, strain ABTS-351 (11A), and spinetoram (5). This rapid bioassay provided the grower with a ranking of insecticide efficacy for the control the DBM population for that farm site. These data allowed growers to make an informed decision on control quickly and plan for resistance management rotations for DBM that season., (© The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2020
- Full Text
- View/download PDF
7. Silencing hepatic MCJ attenuates non-alcoholic fatty liver disease (NAFLD) by increasing mitochondrial fatty acid oxidation.
- Author
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Barbier-Torres L, Fortner KA, Iruzubieta P, Delgado TC, Giddings E, Chen Y, Champagne D, Fernández-Ramos D, Mestre D, Gomez-Santos B, Varela-Rey M, de Juan VG, Fernández-Tussy P, Zubiete-Franco I, García-Monzón C, González-Rodríguez Á, Oza D, Valença-Pereira F, Fang Q, Crespo J, Aspichueta P, Tremblay F, Christensen BC, Anguita J, Martínez-Chantar ML, and Rincón M
- Subjects
- Adult, Aged, Animals, Datasets as Topic, Diet, High-Fat adverse effects, Disease Models, Animal, Female, HSP40 Heat-Shock Proteins antagonists & inhibitors, HSP40 Heat-Shock Proteins genetics, Hepatocytes cytology, Hepatocytes drug effects, Hepatocytes metabolism, Humans, Liver cytology, Liver drug effects, Male, Middle Aged, Mitochondria metabolism, Mitochondrial Proteins antagonists & inhibitors, Mitochondrial Proteins genetics, Molecular Chaperones antagonists & inhibitors, Molecular Chaperones genetics, Nanoparticles administration & dosage, Non-alcoholic Fatty Liver Disease etiology, Non-alcoholic Fatty Liver Disease pathology, Oxidation-Reduction drug effects, Primary Cell Culture, RNA, Small Interfering administration & dosage, RNA-Seq, Fatty Acids metabolism, HSP40 Heat-Shock Proteins metabolism, Liver pathology, Mitochondria drug effects, Mitochondrial Proteins metabolism, Molecular Chaperones metabolism, Non-alcoholic Fatty Liver Disease drug therapy
- Abstract
Nonalcoholic fatty liver disease (NAFLD) is considered the next major health epidemic with an estimated 25% worldwide prevalence. No drugs have yet been approved and NAFLD remains a major unmet need. Here, we identify MCJ (Methylation-Controlled J protein) as a target for non-alcoholic steatohepatitis (NASH), an advanced phase of NAFLD. MCJ is an endogenous negative regulator of the respiratory chain Complex I that acts to restrain mitochondrial respiration. We show that therapeutic targeting of MCJ in the liver with nanoparticle- and GalNAc-formulated siRNA efficiently reduces liver lipid accumulation and fibrosis in multiple NASH mouse models. Decreasing MCJ expression enhances the capacity of hepatocytes to mediate β-oxidation of fatty acids and minimizes lipid accumulation, which results in reduced hepatocyte damage and fibrosis. Moreover, MCJ levels in the liver of NAFLD patients are elevated relative to healthy subjects. Thus, inhibition of MCJ emerges as an alternative approach to treat NAFLD.
- Published
- 2020
- Full Text
- View/download PDF
8. Sonoporation as an Approach for siRNA delivery into T cells.
- Author
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Karki A, Giddings E, Carreras A, Champagne D, Fortner K, Rincon M, and Wu J
- Subjects
- Animals, Blotting, Western, Flow Cytometry, Humans, In Vitro Techniques, Mice, Models, Animal, RNA, Small Interfering administration & dosage, Sonication methods, T-Lymphocytes
- Abstract
Delivery of small interfering RNAs (siRNAs) into primary T cells is quite challenging because they are non-proliferating cells and are difficult to transfect with non-viral approaches. Because sonoporation is independent of the proliferation status of cells and siRNA acts in the cell cytoplasm, we investigated whether sonoporation could be used to deliver siRNA into mouse and human T cells. Cells mixed with Definity microbubbles and siRNA were sonicated with a non-focused transducer of center frequency 2.20 MHz producing ultrasound at a 10% duty cycle, pulse repetition frequency of 2.20 kHz and spatial average temporal average ultrasound intensity of 1.29 W/cm
2 for 5 s and then examined for siRNA fluorescence by flow cytometry analysis. These sonoporation conditions resulted in high-efficiency transfection of siRNA in mouse and human T cells. Further, the efficacy of siRNA delivery by sonoporation was illustrated by the successful visualization of decreased methylation-controlled J protein expression in mouse and human CD8 T cells via Western blot analysis. The results provide the first evidence that sonoporation is a novel approach to delivery of siRNA into fresh isolated mouse and human T cells in vitro, and might be used for in vivo studies in the future., (Copyright © 2019. Published by Elsevier Inc.)- Published
- 2019
- Full Text
- View/download PDF
9. miR-873-5p targets mitochondrial GNMT-Complex II interface contributing to non-alcoholic fatty liver disease.
- Author
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Fernández-Tussy P, Fernández-Ramos D, Lopitz-Otsoa F, Simón J, Barbier-Torres L, Gomez-Santos B, Nuñez-Garcia M, Azkargorta M, Gutiérrez-de Juan V, Serrano-Macia M, Rodríguez-Agudo R, Iruzubieta P, Anguita J, Castro RE, Champagne D, Rincón M, Elortza F, Arslanow A, Krawczyk M, Lammert F, Kirchmeyer M, Behrmann I, Crespo J, Lu SC, Mato JM, Varela-Rey M, Aspichueta P, Delgado TC, and Martínez-Chantar ML
- Subjects
- Adult, Animals, Antagomirs metabolism, Antagomirs therapeutic use, Disease Models, Animal, Electron Transport Complex II genetics, Female, Glycine N-Methyltransferase deficiency, Glycine N-Methyltransferase genetics, Hepatocytes cytology, Hepatocytes metabolism, Humans, Lipid Peroxidation, Liver metabolism, Male, Mice, Mice, Inbred C57BL, MicroRNAs antagonists & inhibitors, MicroRNAs genetics, Middle Aged, Mitochondria metabolism, Non-alcoholic Fatty Liver Disease drug therapy, Non-alcoholic Fatty Liver Disease metabolism, Up-Regulation, Electron Transport Complex II metabolism, Glycine N-Methyltransferase metabolism, MicroRNAs metabolism, Non-alcoholic Fatty Liver Disease pathology
- Abstract
Objective: Non-alcoholic fatty liver disease (NAFLD) is a complex pathology in which several dysfunctions, including alterations in metabolic pathways, mitochondrial functionality and unbalanced lipid import/export, lead to lipid accumulation and progression to inflammation and fibrosis. The enzyme glycine N-methyltransferase (GNMT), the most important enzyme implicated in S-adenosylmethionine catabolism in the liver, is downregulated during NAFLD progression. We have studied the mechanism involved in GNMT downregulation by its repressor microRNA miR-873-5p and the metabolic pathways affected in NAFLD as well as the benefit of recovery GNMT expression., Methods: miR-873-5p and GNMT expression were evaluated in liver biopsies of NAFLD/NASH patients. Different in vitro and in vivo NAFLD murine models were used to assess miR-873-5p/GNMT involvement in fatty liver progression through targeting of the miR-873-5p as NAFLD therapy., Results: We describe a new function of GNMT as an essential regulator of Complex II activity in the electron transport chain in the mitochondria. In NAFLD, GNMT expression is controlled by miR-873-5p in the hepatocytes, leading to disruptions in mitochondrial functionality in a preclinical murine non-alcoholic steatohepatitis (NASH) model. Upregulation of miR-873-5p is shown in the liver of NAFLD/NASH patients, correlating with hepatic GNMT depletion. Importantly, NASH therapies based on anti-miR-873-5p resolve lipid accumulation, inflammation and fibrosis by enhancing fatty acid β-oxidation in the mitochondria. Therefore, miR-873-5p inhibitor emerges as a potential tool for NASH treatment., Conclusion: GNMT participates in the regulation of metabolic pathways and mitochondrial functionality through the regulation of Complex II activity in the electron transport chain. In NAFLD, GNMT is repressed by miR-873-5p and its targeting arises as a valuable therapeutic option for treatment., (Copyright © 2019 The Authors. Published by Elsevier GmbH.. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
10. Reducing Dental Plaque Scores in Long-term Care Facilities Using a Checklist and Random Inspections: A Pilot Study.
- Author
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Laurence B, Rogers I, Grant-Mills D, Smith D, Tefera E, Barbarinde A, Champagne D, and Thomas M
- Subjects
- Aged, Checklist, Humans, Long-Term Care, Pilot Projects, Prospective Studies, Dental Plaque
- Abstract
The development and use of tailored interventions in overcoming barriers to optimum health in long-term care facility residents is of the highest importance. One successful approach to improving health outcomes is the use of a checklist by health care providers. Despite the evidence of the success of such checklists in nursing and medicine, there is little evidence on their use in improving dental outcomes. This study investigated whether an intervention comprising the daily use of a checklist for oral care by nursing staff supplemented by random inspections by a charge nurse resulted in lower dental plaque scores in patient participants at a long-term care facility (n=19) as compared with in those at another long-term care facility that did not receive the intervention (n=13). All participants received a dental cleaning at baseline. At a follow-up examination at 6 to 8 weeks post-baseline, significant differences were observed in the plaque scores between the participants at each location, with the median plaque scores in those undergoing the intervention being less than half of those in the patients that did not (20.8% vs. 52.8%, p<0.001). After adjusting for age in a linear regression model, this difference remained significant. The use of a daily checklist for oral care supplemented by random inspections by a charge nurse was associated with lower plaque scores (p<0.001). These results warrant further research, including prospective studies aimed at establishing how use of both clinical supervision and a checklist for oral health may influence plaque scores in geriatric patients in long-term care facilities over time.
- Published
- 2019
- Full Text
- View/download PDF
11. Marine Leech Anticoagulant Diversity and Evolution.
- Author
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Tessler M, Marancik D, Champagne D, Dove A, Camus A, Siddall ME, and Kvist S
- Subjects
- Animals, Anticoagulants chemistry, Anticoagulants classification, Biodiversity, Biological Evolution, DNA, Complementary chemistry, DNA, Complementary metabolism, Fishes parasitology, High-Throughput Nucleotide Sequencing, Host-Parasite Interactions, Leeches classification, Leeches enzymology, Leeches genetics, Open Reading Frames, Phylogeny, Salivary Glands anatomy & histology, Salivary Glands enzymology, Salivary Glands metabolism, Turtles parasitology, Exome Sequencing, Anticoagulants metabolism, Leeches metabolism, Transcriptome
- Abstract
Leeches (Annelida: Hirudinea) possess powerful salivary anticoagulants and, accordingly, are frequently employed in modern, authoritative medicine. Members of the almost exclusively marine family Piscicolidae account for 20% of leech species diversity, and they feed on host groups (e.g., sharks) not encountered by their freshwater and terrestrial counterparts. Moreover, some species of Ozobranchidae feed on endangered marine turtles and have been implicated as potential vectors for the tumor-associated turtle herpesvirus. In spite of their ecological importance and unique host associations, there is a distinct paucity of data regarding the salivary transcriptomes of either of these families. Using next-generation sequencing, we profiled transcribed, putative anticoagulants and other salivary bioactive compounds that have previously been linked to blood feeding from 7 piscicolid species (3 elasmobranch feeders; 4 non-cartilaginous fish feeders) and 1 ozobranchid species (2 samples). In total, 149 putative anticoagulants and bioactive loci were discovered in varying constellations throughout the different samples. The putative anticoagulants showed a broad spectrum of described antagonistic pathways, such as inhibition of factor Xa and platelet aggregation, which likely have similar bioactive roles in marine fish and turtles. A transcript with homology to ohanin, originally isolated from king cobras, was found in Cystobranchus vividus but is otherwise unknown from leeches. Estimation of selection pressures for the putative anticoagulants recovered evidence for both positive and purifying selection along several isolated branches in the gene trees, and positive selection was also estimated for a few select codons in a variety of marine species. Similarly, phylogenetic analyses of the amino acid sequences for several anticoagulants indicated divergent evolution.
- Published
- 2018
- Full Text
- View/download PDF
12. Effect of Hippotherapy on Motor Proficiency and Function in Children with Cerebral Palsy Who Walk.
- Author
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Champagne D, Corriveau H, and Dugas C
- Subjects
- Child, Child, Preschool, Female, Humans, Male, Prospective Studies, Time Factors, Walking physiology, Cerebral Palsy physiopathology, Cerebral Palsy rehabilitation, Equine-Assisted Therapy, Motor Skills, Muscle Strength, Postural Balance
- Abstract
Aims: To evaluate the effects of hippotherapy on physical capacities of children with cerebral palsy., Methods: Thirteen children (4-12 years old) with cerebral palsy classified in Gross Motor Function Classification System Level I or II were included in this prospective quasi-experimental ABA design study. Participants received 10 weeks of hippotherapy (30 min per week). Gross motor function and proficiency were measured with the Bruininks-Oseretski Motor Proficiency short form [BOT2-SF]) and the Gross Motor Function Measure-88 [GMFM-88] (Dimension D and E) twice before the program (T1 and T1'), immediately after (T2), and 10 weeks following the end of the program (T3)., Results: Mean scores for dimensions D and E of the GMFM-88 Dimension scores (p = .005) and three out of the eight items of the BOT2-SF (fine motor precision (p = .013), balance (p = .025), and strength (p = .012) improved between baseline and immediately after intervention; mean scores immediately following and 10 weeks following intervention did not differ., Conclusions: Hippotherapy provided by a trained therapist who applies an intense and graded session for 10 weeks can improve body functions and performance of gross motor and fine motor activities in children with cerebral palsy.
- Published
- 2017
- Full Text
- View/download PDF
13. Emotional inertia and external events: The roles of exposure, reactivity, and recovery.
- Author
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Koval P, Brose A, Pe ML, Houben M, Erbas Y, Champagne D, and Kuppens P
- Subjects
- Adolescent, Affect, Depression psychology, Female, Humans, Individuality, Male, Models, Psychological, Young Adult, Emotional Adjustment, Emotions
- Abstract
Increased moment-to-moment predictability, or inertia, of negative affect has been identified as an important dynamic marker of psychological maladjustment, and increased vulnerability to depression in particular. However, little is known about the processes underlying emotional inertia. The current article examines how the emotional context, and people's responses to it, are related to emotional inertia. We investigated how individual differences in the inertia of negative affect (NA) are related to individual differences in exposure, reactivity, and recovery from emotional events, in daily life (assessed using experience sampling) as well as in the lab (assessed using an emotional film-clip task), among 200 participants commencing their first year of tertiary education. This dual-method approach allowed us to assess affective responding on different timescales, and in response to standardized as well as idiographic emotional stimuli. Our most consistent finding, across both methods, was that heightened NA inertia is related to decreased NA recovery following negative stimuli, suggesting that higher levels of inertia may be mostly driven by impairments in affect repair following negative events., ((c) 2015 APA, all rights reserved).)
- Published
- 2015
- Full Text
- View/download PDF
14. Updating in working memory predicts greater emotion reactivity to and facilitated recovery from negative emotion-eliciting stimuli.
- Author
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Pe ML, Koval P, Houben M, Erbas Y, Champagne D, and Kuppens P
- Abstract
That emotions change in response to emotion-eliciting events is a natural part of human life. However, it is equally important for emotions to return to baseline once the emotion-eliciting events have passed. This suggests that the ability to emotionally react to and recover from emotion-eliciting events is critical for healthy psychological functioning. But why do individuals differ in their emotion reactivity and recovery? The present work postulates that the ability to update emotional information in working memory (WM) may explain individual differences in emotion reactivity and recovery. Two studies are presented, which examined whether updating ability was related to emotion reactivity and recovery. In Study 1, we assessed participants' self-reported affect as they viewed negative and positive films. Our results revealed that better updating ability was related to greater emotion reactivity and facilitated (i.e., quicker) recovery from watching negative films. In Study 2, participants recalled a recent angering event, and were then instructed to either ruminate about or reappraise the event. Results revealed that updating ability was again related to greater emotion reactivity and facilitated (i.e., successful) emotion recovery in response to the angering event, and that this was unrelated to the emotion regulation strategy used. These findings identify the ability to update emotional information in WM as a possible mechanism in emotion responding.
- Published
- 2015
- Full Text
- View/download PDF
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