29 results on '"Garzón Tiznado, José Antonio"'
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2. Cross pathogenicity of Fusarium oxysporum isolated from peppers
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López-Orona, Carlos Alfonso, Hernández-Verdugo, Sergio, Velarde-Félix, Sixto, Garzón-Tiznado, José Antonio, Sy, Ousmane, and Retes-Manjarrez, Jesús Enrique
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- 2019
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3. Plant characterization of genetically modified maize hybrids MON-89Ø34-3 × MON-88Ø17-3, MON-89Ø34-3 × MON-ØØ6Ø3-6, and MON-ØØ6Ø3-6: alternatives for maize production in Mexico
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Heredia Díaz, Oscar, Aldaba Meza, José Luis, Baltazar, Baltazar M., Bojórquez Bojórquez, Germán, Castro Espinoza, Luciano, Corrales Madrid, José Luis, de la Fuente Martínez, Juan Manuel, Durán Pompa, Héctor Abel, Alonso Escobedo, José, Espinoza Banda, Armando, Garzón Tiznado, José Antonio, González García, Juvencio, Guzmán Rodríguez, José Luis, Madueño Martínez, Jesús Ignacio, Martínez Carrillo, José Luis, Meng, Chen, Quiñones Pando, Francisco Javier, Rosales Robles, Enrique, Ruiz Hernández, Ignacio, Treviño Ramírez, José Elías, Uribe Montes, Hugo Raúl, and Zavala García, Francisco
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- 2017
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4. Functional gluten-free beverage elaborated from whole quinoa and defatted chia extruded flours: antioxidant and antihypertensive potentials
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Sánchez Magaña, Luis Martín, primary, Reyes Moreno, Cuauhtémoc, additional, Garzón Tiznado, José Antonio, additional, Milán Carrillo, Jorge, additional, Gómez Favela, Mario Armando, additional, Cuevas Rodríguez, Edith Oliva, additional, Espinoza Moreno, Ramona Julieta, additional, and Gutiérrez Dorado, Roberto, additional
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- 2022
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5. Resistance to Bemisia tabaci (Hemiptera: Aleyrodidae) Mediterranean (Q Biotype) in Landrace and Wild Tomato Populations from Mexico
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Millán-Chaidez, Reynaldo, primary, Garzón-Tiznado, José Antonio, additional, Linares-Flores, Perla Judith, additional, Velarde-Félix, Sixto, additional, Lugo-García, Gabriel Antonio, additional, and Retes-Manjarrez, Jesús Enrique, additional
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- 2021
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6. Phylogenetic Analyses of the Complex of Endosymbionts in Bemisia tabaci1 in the Valley of Culiacan
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Linares-Flores, Perla Judith, primary, Rosario León-Sicairos, Claudia Del, additional, López-Valenzuela, José Ángel, additional, Pacheco-Arjona, José Ramón, additional, Pineda-Hidalgo, Karen Virginia, additional, Retes-Manjarrez, Jesús Enrique, additional, and Garzón-Tiznado, José Antonio, additional
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- 2021
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7. Antixenosis a Bemisia tabaci1 Mediterranean en Poblaciones Criollas y Silvestres de Tomate de México
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Garzón-Tiznado, José Antonio, primary, Lugo-Lujan, Jonathan Misael, additional, Hernández-Verdugo, Sergio, additional, Medina-López, Raymundo, additional, Velarde-Félix, Sixto, additional, Portillo-Loera, Jesús José, additional, and Retes-Manjarrez, Jesús Enrique, additional
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- 2020
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8. Inheritance of Resistance to Pepper huasteco yellow vein virus in Capsicum annuum L.
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Retes-Manjarrez, Jesús Enrique, primary, Hernández-Verdugo, Sergio, additional, López-Orona, Carlos Alfonso, additional, Medina-López, Raymundo, additional, Garzón-Tiznado, José Antonio, additional, and Retes-Cázarez, Jesús Enrique, additional
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- 2019
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9. Corrigendum: Iron responsive-like elements in the parasite Entamoeba histolytica
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Soto-Castro, Liliana, primary, Plata-Guzmán, Laura Yuliana, additional, Figueroa-Angulo, Elisa Elvira, additional, Calla-Choque, Jaeson Santos, additional, Reyes-López, Magda, additional, de la Garza, Mireya, additional, León-Sicairos, Nidia, additional, Garzón-Tiznado, José Antonio, additional, Arroyo, Rossana, additional, and León-Sicairos, Claudia, additional
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- 2019
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10. Identificación molecular de Fusarium spp. aislados de maíz en Sinaloa, México
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Velarde Felix, Sixto, primary, Valdez Rubio, Nallely, additional, Zamora Galván, Francisco, additional, López Molina, Ramón, additional, Melgoza Villagómez, Claudia María, additional, and Garzón Tiznado, José Antonio, additional
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- 2018
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11. Presencia de 'Candidatus Liberibacter solanacearum' en 'Bactericera cockerelli' Sulc asociada con enfermedades en tomate, chile y papa
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Melgoza Villagómez, Claudia Maria, León Sicairos, Claudia del Rosario, López Valenzuela, José Angel, Hernández Espinal, Luis Alberto, Velarde Félix, Sixto, Garzón Tiznado, José Antonio, Melgoza Villagómez, Claudia Maria, León Sicairos, Claudia del Rosario, López Valenzuela, José Angel, Hernández Espinal, Luis Alberto, Velarde Félix, Sixto, and Garzón Tiznado, José Antonio
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The “permanent tomato”, “spotted tuber”or “zebra chip”in potato and “chlorotic buds”of chili, are three diseases described in Mexico with coincidental signs of flower abortion, obscuration of vascular tissue at the base of the stem and root of the plants. The association between these diseases and the emerging bacterium Candidatus Liberibactersolanacearum(CLs) as well as the psyllid Bactericera cockerelli as its vector has been mentioned. These diseases, which were initially located in three states of Mexico, have spread to the main Solanaceae producing regions, both in field conditions and in the greenhouse. The objective of the study was to know the presence of CLs associated with diseases that affect the tomato, para and chilicrops in Mexico. The bacterium was identified by PCR of the 16S rDNA gene, cloning and sequencing. The alignment of nucleotide sequences was performed with the Clustal W method and the phylogenetic tree was built with the Neighbor-Joining algorithm from distances calculated with the Tajima-Nei method and a Felsenstein index of 1000 replicas, using the software MEGA version 5.05. A total of 167 samples were analyzed, of which 86 were positive, from 14 states of Mexico. Five nucleotide sequences from Guanajuato, San Luis Potosiand Sinaloa corresponding to tomato, potato and chili, adults and eggs of B. cockerelli were obtained. The analysis of the sequences showed an identity of 99.4%when compared between these and up to 99.8% with GenBank accessions described for CLs in the USA, New Zealand and Canada. Keywords: chlorotic buds, tomato permanent, zebra chip, El “permanente del tomate”, “manchado del tubérculo” o “zebra chip” en papa y “brotes cloróticos” del chile, son tres enfermedades descritas en México con signos coincidentes de aborto de flor, oscurecimiento de tejido vascular en la base del tallo y raíz de las plantas. Se ha mencionado la asociación entre estas enfermedades y la bacteria emergente Candidatus Liberibacter solanacearum (CLs) así como al psílido Bactericera cockerelli como su vector. Estas enfermedades, que en inicio se localizaban en tres estados de México se han diseminado a las principales regiones productoras de solanáceas, tanto en condiciones de campo como en invernadero. El objetivo del estudio fue conocer la presencia de CLs asociado a enfermedades que afectan los cultivos de tomate, para y chile en México. La bacteria se identificó por PCR del gen 16S de ADNr, clonación y secuenciación. La alineación de secuencias nucleotídicas se realizó con el método Clustal W y el árbol filogenético se construyó con el algoritmo de Neighbor-Joining a partir de distancias calculadas con el método de Tajima-Nei y un índice de Felsenstein de 1 000 réplicas, utilizando el software MEGA versión 5.05. En total se analizaron 167 muestras, de las cuales 86 resultaron positivas, provenientes de 14 estados de México. Se obtuvieron cinco secuencias nucleotídicas de Guanajuato, San Luis Potosí y Sinaloa correspondientes al tomate, papa y chile, adultos y huevecillos de B. cockerelli. El análisis de las secuencias mostró una identidad de 99.4% al comparase entre estas y hasta 99.8% con accesiones del GenBank descritas para CLs en EUA, Nueva Zelanda y Canadá. Palabras clave: brotes cloróticos, permanente del tomate, zebra chip
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- 2018
12. Transportability of non-target arthropod field data for the use in environmental risk assessment of genetically modified maize in Northern Mexico
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Corrales Madrid, José Luis, Martínez Carrillo, José Luis, Osuna Martínez, M. B., Durán Pompa, Héctor Abel, Alonso Escobedo, José, Javier Quiñones, F., Garzón Tiznado, José Antonio, Castro Espinoza, Luciano, Zavala García, Francisco, Espinoza Banda, Armando, González García, Juvencio, Jiang, C., Brown, C. R., De la F. Martínez, J. M., Heredia Díaz, Oscar, Whitsel, J. E., Asiimwe, P., Baltazar, Baltazar M., Ahmad, A., Corrales Madrid, José Luis, Martínez Carrillo, José Luis, Osuna Martínez, M. B., Durán Pompa, Héctor Abel, Alonso Escobedo, José, Javier Quiñones, F., Garzón Tiznado, José Antonio, Castro Espinoza, Luciano, Zavala García, Francisco, Espinoza Banda, Armando, González García, Juvencio, Jiang, C., Brown, C. R., De la F. Martínez, J. M., Heredia Díaz, Oscar, Whitsel, J. E., Asiimwe, P., Baltazar, Baltazar M., and Ahmad, A.
- Abstract
In country, non-target arthropod (NTA) field evaluations are required to comply with the regulatory process for cultivation of genetically modified (GM) maize in Mexico. Two sets of field trials, Experimental Phase and Pilot Phase, were conducted to identify any potential harm of insect-protected and glyphosate-tolerant maize (MON89Ø34-3 × MON-88Ø17-3 and MON-89Ø34-3 × MON-ØØ6Ø3-6) and glyphosatetolerant maize (MON-ØØ6Ø3-6) to local NTAs compared to conventional maize. NTA abundance data were collected at 32 sites, providing high geographic and environmental diversity within maize production areas from four ecological regions (ecoregions) in northern Mexico. The most abundant herbivorous taxa collected included field crickets, corn flea beetles, rootworm beetles, cornsilk flies, aphids, leafhoppers, plant bugs and thrips while the most abundant beneficial taxa captured were soil mites, spiders, predatory ground beetles, rove beetles, springtails (Collembola), predatory earwigs, ladybird beetles, syrphid flies, tachinid flies, minute pirate bugs, parasitic wasps and lacewings. Across the taxa analysed, no statistically significant differences in abundance were detected between GM maize and the conventional maize control for 69 of the 74 comparisons (93.2%) indicating thatthe single or stacked insect-protected and herbicide-tolerant GM traits generally exert no marked adverse effects on the arthropod populations compared with conventional maize. The distribution of taxa observed in this study provides evidence that irrespective of variations in overall biodiversity of a given ecoregion, important herbivore, predatory and parasitic arthropod taxa within the commercial maize agroecosystem are highly similar indicating that relevant data generated in one ecoregion can be transportable for the risk assessment of the same or similar GM crop in another ecoregion.
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- 2018
13. Resistance to Candidatus Liberibacter solanacearum haplotype B in tomato landraces from Mexico.
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Garzón‐Tiznado, José Antonio, Melgoza‐Villagómez, Claudia María, López‐Orona, Carlos Alfonso, Castro‐Espinoza, Luciano, Velarde‐Félix, Sixto, Figueroa‐Pérez, Marely Graciela, and Retes‐Manjarrez, Jesús Enrique
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CANDIDATUS , *BACTERIAL wilt diseases , *TOMATO diseases & pests , *GERMPLASM , *SYMPTOMS , *TOMATOES - Abstract
Candidatus Liberibacter solanacearum haplotype B (CLsoB) is an economically important pathogen of tomato (Solanum lycopersicum L.) crops in New Zealand and Central and North America. Currently, resistant cultivars of tomato are not available as a management tactic because breeding programmes lack sources of resistance. Therefore, the objective of this study was to identify sources of resistance in tomato to CLsoB. Forty‐six landraces of tomato were collected from several states in Mexico and were inoculated with CLsoB using 20 infested adults of Bactericera cockerelli per plant. Two assays were done over two years under greenhouse conditions. In the first trial, landraces FC22 and FC44 showed a significantly higher proportion of resistant plants, less symptom severity and longer incubation time compared with the other forty‐four landraces and two susceptible cultivars. In the second assay, resistance to CLsoB of the landraces FC22 and FC44 was confirmed because they had again significantly greater numbers of resistant plants, less symptom severity, relative lower CLsoB titers and longer incubation time relative to the other genotypes. All plants considered resistant from both assays had DNA of CLsoB. Results indicate that all resistant plants from these landraces are promising sources for the development of tomato cultivars with resistance to CLsoB. [ABSTRACT FROM AUTHOR]
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- 2020
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14. Antixenosis a Bemisia tabaci Mediterranean en Poblaciones Criollas y Silvestres de Tomate de México.
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Garzón-Tiznado, José Antonio, Lugo-Lujan, Jonathan Misael, Hernández-Verdugo, Sergio, Medina-López, Raymundo, Velarde-Félix, Sixto, Portillo-Loera, Jesús José, and Retes-Manjarrez, Jesús Enrique
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SWEETPOTATO whitefly , *TOMATOES , *TOMATO varieties , *TRICHOMES - Abstract
Se estudió bajo condiciones controladas en invernadero el índice de atracción de adultos y preferencia de oviposición de Bemisia tabaci Mediterranean en ocho poblaciones criollas (Solanum lycopersicum) y catorce poblaciones silvestres (S. lycopersicum var. cerasiforme) de tomate, así como la relación de estos parámetros de resistencia tipo antixenosis con la densidad de tricomas no-glandulares que son características que pueden prevenir la infestación de este insecto. Cinco poblaciones silvestres y tres criollas mostraron una resistencia alta, y tres poblaciones criollas y una silvestre tuvieron una resistencia intermedia. El resto de las poblaciones y un cultivar comercial fueron altamente susceptibles porque fueron significativamente más atraídos por los adultos y preferidos para la oviposición de este insecto, indicando que existen diferentes niveles de resistencia tipo antixenosis. El número de tricomas no-glandulares no se correlacionó con ninguno de los parámetros de resistencia, indicando que no existe relación entre estos parámetros y que la resistencia observada en estos genotipos puede ser debida a otros factores como compuestos volátiles que repelen a este insecto. Estas poblaciones de tomates con diferentes niveles de resistencia tipo antixenosis pueden utilizarse para producir cultivares de tomate menos atractivas para la infestación de este insecto y como barreras físicas que rodeen los cultivos de tomate para repeler las infestaciones de B. tabaci Mediterranean. The index of attraction of adults and oviposition preference index of Bemisia tabaci Mediterranean in eight landraces populations (Solanum lycopersicum) and 14 wild populations (S. lycopersicum var. cerasiforme) of tomato was studied under greenhouse conditions, as well as the relationship of these parameters of antixenosis resistance with the density of non-glandular trichomes that are a trait that can prevent infestations of this insect. Five wild and three landraces tomato populations showed high resistance, and three landraces and one wild populations had intermediate resistance. Other populations and a commercial cultivar were very susceptible because they were significantly more attractive for adults and preferred them for oviposition. The results indicate that there are different levels of antixenosis resistance in these tomato populations. The number of non-glandular trichomes did not correlate with any of the resistance parameters, indicating there is no relationship between these parameters and that the resistance observed in these genotypes may be due to other factors such as volatile compounds that repel this insect. These tomato populations with different levels of antixenosis-type resistance can be used to generate less attractive tomato cultivars for this insect and as physical barriers that surround tomato crops to repel B. tabaci Mediterranean infestations. [ABSTRACT FROM AUTHOR]
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- 2020
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15. Presencia de Candidatus Liberibacter solanacearum en Bactericera cockerelli Sulc asociada con enfermedades en tomate, chile y papa
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Melgoza Villagómez, Claudia María, primary, León Sicairos, Claudia del Rosario, additional, López Valenzuela, José Ángel, additional, Hernández Espinal, Luis Alberto, additional, Velarde Félix, Sixto, additional, and Garzón Tiznado, José Antonio, additional
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- 2018
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16. Resistance to Pepper huasteco yellow vein virus and its heritability in wild genotypes of Capsicum annuum
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Retes-Manjarrez, Jesús Enrique, primary, Hernández-Verdugo, Sergio, additional, Pariaud, Benedicte, additional, Hernández-Espinal, Luis Alberto, additional, Parra-Terraza, Saúl, additional, Trejo-Saavedra, Diana Lilia, additional, Rivera-Bustamante, Rafael Francisco, additional, and Garzón-Tiznado, José Antonio, additional
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- 2018
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17. Occurrence of Fusarium oxysporum causing wilt on pepper in Mexico
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Velarde-Félix, Sixto, primary, Garzón-Tiznado, José Antonio, additional, Hernández-Verdugo, Sergio, additional, López-Orona, Carlos Alfonso, additional, and Retes-Manjarrez, Jesús Enrique, additional
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- 2018
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18. Heritability of the resistance to pepper huasteco yellow vein virus in wild genotypes of Capsicum annuum
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Retes-Manjarrez, Jesús Enrique, primary, Hernández-Verdugo, Sergio, additional, Evrard, Alexandre, additional, and Garzón-Tiznado, José Antonio, additional
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- 2017
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19. Ultrasound-assisted extraction of fructans from agave (Agave tequilana Weber var. azul) at different ultrasound powers and solid-liquid ratios
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Sánchez Madrigal, Miguel Ángel, Amaya Guerra, Carlos Abel, Quintero Ramos, Armando, Báez González, Juan Gabriel, Núñez González, María Adriana, Ruiz Gutiérrez, Martha Graciela, Garzón Tiznado, José Antonio, Sánchez Madrigal, Miguel Ángel, Amaya Guerra, Carlos Abel, Quintero Ramos, Armando, Báez González, Juan Gabriel, Núñez González, María Adriana, Ruiz Gutiérrez, Martha Graciela, and Garzón Tiznado, José Antonio
- Abstract
The effects of ultrasound-assisted extraction (UAE) at different ultrasound power densities (UPDs; 40, 80, and 120 mW/mL) and solid:liquid (S:L) ratio (1:2, 1:3, and 1:6) on the extraction of carbohydrates from Agave tequilana plant of different ages were evaluated. Extracts obtained (6- and 7-year-old plant) were analyzed in the yield of carbohydrates (YC), fructan (FRU) content, simple sugars, fructan profile and the average degree of polymerization (DPn). UPD, S:L ratio, and plant age all affected YC, FRU, and DPn. Maximum YC and FRU were obtained from the older agave with UPD and S:L ratio of 120 mW/mL and 1:6, respectively; while glucose, fructose, and sucrose were highly released from the younger plant. Agave of 7-year-old presented the highest DPn. Fructan degradation occurred at high UPD, increasing the simple sugars and decreasing the DPn. Thermal-traditional extraction without sonication caused more fructan degradation; and overall, ultrasound enhanced fructan extraction and minimized fructan damage, representing a technological alternative for fructan extraction from agave. Keywords: agave; fructans; ultrasound; power density; solid:liquid ratio; plant age. Practical Application: Agave tequilana Weber var. azul plants have significant amounts of fructans. Extraction of these components by alternative methods such as ultrasound could represent advantages, improving the extraction and product quality. This research presents an alternative for the extraction of fructans assisted with ultrasound, evaluating different powers and solid-liquid ratios from agave heads of two different ages. Both variables, as well as age of agave showed a strong effect on fructan extraction. Ultrasound enhanced the extraction and minimized fructan damage, representing a technological alternative.
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- 2017
20. Iron responsive-like elements in the parasite Entamoeba histolytica
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Soto-Castro, Liliana, primary, Plata-Guzmán, Laura Yuliana, additional, Figueroa-Angulo, Elisa Elvira, additional, Calla-Choque, Jaeson Santos, additional, Reyes-López, Magda, additional, de la Garza, Mireya, additional, León-Sicairos, Nidia, additional, Garzón-Tiznado, José Antonio, additional, Arroyo, Rossana, additional, and León-Sicairos, Claudia, additional
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- 2017
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21. Ultrasound-assisted extraction of fructans from agave (Agave tequilana Weber var. azul) at different ultrasound powers and solid-liquid ratios
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SÁNCHEZ-MADRIGAL, Miguel Ángel, primary, AMAYA-GUERRA, Carlos Abel, additional, QUINTERO-RAMOS, Armando, additional, BÁEZ-GONZÁLEZ, Juan Gabriel, additional, NÚÑEZ-GONZÁLEZ, María Adriana, additional, RUIZ-GUTIÉRREZ, Martha Graciela, additional, and GARZÓN-TIZNADO, José Antonio, additional
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- 2017
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22. Molecular identification of Fusarium spp. isolated maize in Sinaloa, Mexico.
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Velarde Félix, Sixto, Valdez Rubio, Nallely, Galván, Francisco Zamora, López Molina, Ramón, Melgoza Villagómez, Claudia María, and Garzón Tiznado, José Antonio
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CORN disease & pest resistance ,FUSARIUM diseases of plants ,FORAGE plants ,PLANT species ,PHYTOPATHOGENIC microorganisms - Abstract
Fusarium species cause macular diseases in the maize that induce rotting of stems, roots and grains of corn and finally the death of the plant. In the present study, the presence and diversity of Fusarium species associated with these damages in Sinaloa was molecularly identified. During the sowing periods in the years 2013-2014, stem and root tissue from young and adult plants, as well as diseased pod kernels, was collected in the municipalities of Ahome, Culiacan and Elota in the state of Sinaloa. In this investigation, species F. verticillioides, F. oxysporum, F. subglutinans, F. equiseti, F. nygamai, F. cf. bullatum and F. andiyazi, were identified by enzymatic sequencing analysis, where F. verticilloides was the most predominant species. Likewise, the presence in Mexico of Fusarium cf. bullatum causing damage to corn. [ABSTRACT FROM AUTHOR]
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- 2018
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23. Plant characterization of genetically modified maize hybrids MON-89Ø34-3 × MON-88Ø17-3, MON-89Ø34-3 × MON-ØØ6Ø3-6, and MON-ØØ6Ø3-6: alternatives for maize production in Mexico
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Heredia Díaz, Oscar, Aldaba Meza, José Luis, Baltazar, Baltazar M., Bojórquez Bojórquez, Germán, Castro Espinoza, Luciano, Corrales Madrid, José Luis, De la Fuente Martínez, Juan Manuel, Durán Pompa, Héctor Abel, Alonso Escobedo, José, Espinoza Banda, Armando, Garzón Tiznado, José Antonio, González García, Juvencio, Guzmán Rodríguez, José Luis, Madueño Martínez, Jesús Ignacio, Martínez Carrillo, José Luis, Meng, Chen, Quiñones Pando, Francisco Javier, Rosales Robles, Enrique, Ruiz Hernández, Ignacio, Treviño Ramírez, José Elías, Uribe Montes, Hugo Raúl, Zavala García, Francisco, Heredia Díaz, Oscar, Aldaba Meza, José Luis, Baltazar, Baltazar M., Bojórquez Bojórquez, Germán, Castro Espinoza, Luciano, Corrales Madrid, José Luis, De la Fuente Martínez, Juan Manuel, Durán Pompa, Héctor Abel, Alonso Escobedo, José, Espinoza Banda, Armando, Garzón Tiznado, José Antonio, González García, Juvencio, Guzmán Rodríguez, José Luis, Madueño Martínez, Jesús Ignacio, Martínez Carrillo, José Luis, Meng, Chen, Quiñones Pando, Francisco Javier, Rosales Robles, Enrique, Ruiz Hernández, Ignacio, Treviño Ramírez, José Elías, Uribe Montes, Hugo Raúl, and Zavala García, Francisco
- Abstract
Environmental risk assessment (ERA) of genetically modified (GM) crops is a process to evaluate whether the biotechnology trait(s) in a GM crop may result in increased pest potential or harm to the environment. In this analysis, two GM insect-resistant (IR) herbicide-tolerant maize hybrids (MON-89Ø34-3 9 MON-88Ø17-3 and MON-89Ø34-3 9 MON-ØØ6Ø3-6) and one herbicide-tolerant GM hybrid (MON-ØØ6Ø3-6) were compared with conventional maize hybrids of similar genetic backgrounds. Two sets of studies, Experimental Phase and Pilot Phase, were conducted across five ecological regions (ecoregions) in Mexico during 2009–2013, and data were subject to meta-analysis. Results from the Experimental Phase studies, which were used for ERA, indicated that the three GM hybrids were not different from conventional maize for early stand count, days-tosilking, days-to-anthesis, root lodging, stalk lodging, or final stand count. Statistically significant differences were observed for seedling vigor, ear height, plant height, grain moisture, and grain yield, particularly in the IR hybrids; however, none of these phenotypic differences are expected to contribute to a biological or ecological change that would result in an increased pest potential or ecological risk when cultivating these GM hybrids. Overall, results from the Experimental Phase studies are consistent with those from other world regions, confirming that there are no additional risks compared to conventional maize. Results from Pilot Phase studies indicated that, compared to conventional maize hybrids, no differences were detected for the agronomic and phenotypic characteristics measured on the three GM maize hybrids, with the exception of grain moisture and grain yield in the IR hybrids. Since MON-89Ø343 9 MON-88Ø17-3 and MON-89Ø34-3 9 MONØØ6Ø3-6 confer resistance to target insect pests, they are an alternative for farmers in Mexico to protect the crop from insect damage. Additionally, the herbicide tolerance conferred by
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- 2016
24. Plant characterization of genetically modified maize hybrids MON-89Ø34-3 × MON-88Ø17-3, MON-89Ø34-3 × MON-ØØ6Ø3-6, and MON-ØØ6Ø3-6: alternatives for maize production in Mexico
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Heredia Díaz, Oscar, primary, Aldaba Meza, José Luis, additional, Baltazar, Baltazar M., additional, Bojórquez Bojórquez, Germán, additional, Castro Espinoza, Luciano, additional, Corrales Madrid, José Luis, additional, de la Fuente Martínez, Juan Manuel, additional, Durán Pompa, Héctor Abel, additional, Alonso Escobedo, José, additional, Espinoza Banda, Armando, additional, Garzón Tiznado, José Antonio, additional, González García, Juvencio, additional, Guzmán Rodríguez, José Luis, additional, Madueño Martínez, Jesús Ignacio, additional, Martínez Carrillo, José Luis, additional, Meng, Chen, additional, Quiñones Pando, Francisco Javier, additional, Rosales Robles, Enrique, additional, Ruiz Hernández, Ignacio, additional, Treviño Ramírez, José Elías, additional, Uribe Montes, Hugo Raúl, additional, and Zavala García, Francisco, additional
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- 2016
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25. Identificación molecular y biológica de las razas 0 y 5 de 'Fusarium oxysporum' Schlechtend.: Fr f. sp. 'ciceris' (Padwick) Matuo & K. Sato del garbanzo en el noroeste de México
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Velarde Félix, Sixto, Ortega Murrieta, Pedro F, Fierros Leyva, Gustavo A., Padilla Valenzuela, Isidoro, Gutierres Pérez, Erasmo, Rodríguez Cota, Franklin Gerardo, López Valenzuela, José Angel, Acosta Gallegos, Jorge Alberto, Garzón Tiznado, José Antonio, Velarde Félix, Sixto, Ortega Murrieta, Pedro F, Fierros Leyva, Gustavo A., Padilla Valenzuela, Isidoro, Gutierres Pérez, Erasmo, Rodríguez Cota, Franklin Gerardo, López Valenzuela, José Angel, Acosta Gallegos, Jorge Alberto, and Garzón Tiznado, José Antonio
- Abstract
Fusarium oxysporum f. sp. ciceris (Foc) is a phytopathogenic fungus that causes the disease known as Fusarium wilt in chickpea cultivation. In Mexico, chickpea export only grown in the Northwest (Sinaloa, Sonora and Baja California Sur). Considering that Foc limits the production of this crop and no information concerning the identification of physiological races of this fungus in Mexico, the goal was to isolate and identify by molecular and biological tests physiological races of the fungus present in the growing areas chickpea in the northwest region of Mexico. During the period 2010-2014 were collected chickpea plants with symptoms of wilting and yellowing at different locations in the States of Sinaloa, Sonora and Baja California Sur. The fungus was isolated from small portions of the plant, which were seeded in medium potato-dextrose agar culture medium supplemented with pentanitroclorobenceno (PCNB) and chloramphenicol. We isolated and purified spore cultures, DNA was extracted for identification of physiological races by PCR and enzymatic sequencing. These strains were inoculated into chickpea in differential lines, confirming Mexico for the first time the identification of physiological races Foc 0 and 5., Fusarium oxysporum f. sp. ciceris (Foc) es un hongo fitopatógeno que causa la enfermedad conocida como fusariosis vascular en el cultivo del garbanzo. En México, el garbanzo para exportación sólo se cultiva en el Noroeste (Sinaloa, Sonora y Baja California Sur). Considerando que Foc limita la producción de este cultivo y que en México no existe información referente a la identificación de las razas fisiológicas de este hongo, el objetivo fue aislar e identificar mediante pruebas moleculares y biológicas las razas fisiológicas del hongo presentes en las zonas de cultivo de garbanzo de la región del noroeste de México. Durante el periodo 2010-2014 se colectaron plantas de garbanzo con síntomas de marchitez y amarillez en diferentes localidades de los estados de Sinaloa, Sonora y Baja California Sur. El hongo se aisló a partir de pequeñas porciones de la planta, los cuales fueron sembradas en medio de cultivo papa-dextrosa-agar suplementado con pentanitroclorobenceno (PCNB) y cloranfenicol. Se aislaron y purificaron cultivos monospóricos, a los cuales se les extrajo el ADN para la identificación de razas fisiológicas mediante PCR y secuenciación enzimática. Estas cepas se inocularon en líneas diferenciales de garbanzo, confirmándose por primera vez en México la identificación de las razas fisiológicas 0 y 5 de Foc.
- Published
- 2015
26. Pollen-Mediated Gene Flow in Maize: Implications for Isolation Requirements and Coexistence in Mexico, the Center of Origin of Maize
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Peña, Leandro, Baltazar, Baltazar M., Castro Espinoza, Luciano, Espinoza Banda, Armando, De la Fuente Martínez, Juan Manuel, Garzón Tiznado, José Antonio, González García, Juvencio, Gutiérrez, Marco Antonio, Guzmán Rodríguez, José Luis, Heredia Díaz, Oscar, Horak, Michael J., Madueño Martínez, Jesús Ignacio, Schapaugh, Adam W., Stojšin, Duška, Uribe Montes, Hugo Raúl, Zavala García, Francisco, Peña, Leandro, Baltazar, Baltazar M., Castro Espinoza, Luciano, Espinoza Banda, Armando, De la Fuente Martínez, Juan Manuel, Garzón Tiznado, José Antonio, González García, Juvencio, Gutiérrez, Marco Antonio, Guzmán Rodríguez, José Luis, Heredia Díaz, Oscar, Horak, Michael J., Madueño Martínez, Jesús Ignacio, Schapaugh, Adam W., Stojšin, Duška, Uribe Montes, Hugo Raúl, and Zavala García, Francisco
- Abstract
Mexico, the center of origin of maize (Zea mays L.), has taken actions to preserve the identity and diversity of maize landraces and wild relatives. Historically, spatial isolation has been used in seed production to maintain seed purity. Spatial isolation can also be a key component for a strategy to minimize pollen-mediated gene flow in Mexico between transgenic maize and sexually compatible plants of maize conventional hybrids, landraces, and wild relatives. The objective of this research was to generate field maize-to-maize outcrossing data to help guide coexistence discussions in Mexico. In this study, outcrossing rates were determined and modeled from eight locations in six northern states, which represent the most economically important areas for the cultivation of hybrid maize in Mexico. At each site, pollen source plots were planted with a yellow-kernel maize hybrid and surrounded by plots with a white-kernel conventional maize hybrid (pollen recipient) of the same maturity. Outcrossing rates were then quantified by assessing the number of yellow kernels harvested from white-kernel hybrid plots. The highest outcrossing values were observed near the pollen source (12.9% at 1 m distance). The outcrossing levels declined sharply to 4.6, 2.7, 1.4, 1.0, 0.9, 0.5, and 0.5% as the distance from the pollen source increased to 2, 4, 8, 12, 16, 20, and 25 m, respectively. At distances beyond 20 m outcrossing values at all locations were below 1%. These trends are consistent with studies conducted in other world regions. The results suggest that coexistence measures that have been implemented in other geographies, such as spatial isolation, would be successful in Mexico to minimize transgenic maize pollen flow to conventional maize hybrids, landraces and wild relatives.
- Published
- 2015
27. Occurrence of <italic>Fusarium oxysporum</italic> causing wilt on pepper in Mexico.
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Velarde-Félix, Sixto, Garzón-Tiznado, José Antonio, Hernández-Verdugo, Sergio, López-Orona, Carlos Alfonso, and Retes-Manjarrez, Jesús Enrique
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FUSARIUM oxysporum , *ROOT rots , *CHLOROSIS (Plants) , *PEPPERS , *CAPSICUM annuum - Abstract
A new disease on greenhouse pepper (
Capsicum annuum L.) plants in Culiacan, Mexico with symptoms of chlorosis, wilting and vascular necrosis was observed in 2014. Sixty diseased pepper plants were collected from three different areas of the Culiacan Valley to determine the causal agent of the disease and analyse the potential risk presented by this disease in this region.Fusarium oxysporum was recovered consistently (88% of samples collected) and identified based on cultural, morphological and pathogenicity tests, as well as by PCR amplification and DNA sequencing of the TEF-1α gene. Two pathogenicity tests with 10 monoconidial isolates ofF. oxysporum , which were selected randomly from diseased tissues, produced disease symptoms similar to those observed on pepper plants in the greenhouses. Symptoms were different from the typical root rot, wilting and necrosis caused byF. solani andF. oxysporum f. sp.radicis-capsici and instead matched those reported forF. oxysporum f. sp.capsici causing pepper wilt. The results suggest that the causal agent of pepper wilt in the samples analysed wasF. oxysporum . This is the first report of pepper wilt caused by this fungus in Mexico, and this disease is a potential threat for pepper production in this region. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
28. Pollen-Mediated Gene Flow in Maize: Implications for Isolation Requirements and Coexistence in Mexico, the Center of Origin of Maize
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Baltazar, Baltazar M., primary, Castro Espinoza, Luciano, additional, Espinoza Banda, Armando, additional, de la Fuente Martínez, Juan Manuel, additional, Garzón Tiznado, José Antonio, additional, González García, Juvencio, additional, Gutiérrez, Marco Antonio, additional, Guzmán Rodríguez, José Luis, additional, Heredia Díaz, Oscar, additional, Horak, Michael J., additional, Madueño Martínez, Jesús Ignacio, additional, Schapaugh, Adam W., additional, Stojšin, Duška, additional, Uribe Montes, Hugo Raúl, additional, and Zavala García, Francisco, additional
- Published
- 2015
- Full Text
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29. Phylogenetic Analyses of the Complex of Endosymbionts in Bemisia tabaci in the Valley of Culiacan.
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Linares-Flores, Perla Judith, Rosario León-Sicairos, Claudia Del, López-Valenzuela, José Ángel, Pacheco-Arjona, José Ramón, Pineda-Hidalgo, Karen Virginia, Retes-Manjarrez, Jesús Enrique, and Garzón-Tiznado, José Antonio
- Subjects
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PEST control , *CYTOCHROME oxidase , *SWEETPOTATO whitefly , *INSECTICIDE resistance , *TOMATOES , *TOMATO diseases & pests , *MOLECULAR phylogeny - Abstract
Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a major pest of the tomato (Solanum lycopersicum L.) crop worldwide, mostly because of resistance to insecticide that has been associated with endosymbionts. Although knowledge of biotypes in the B. tabaci complex and its endosymbionts is critical for developing pest management strategies, limited information is available on the pest in the Culiacan Province of Mexico. In total, 249 samples of whiteflies were collected from tomato plants of four growers in the Culiacan Valley to analyze the genetic identity of the B. tabaci complex and its endosymbionts. PCR and phylogenetic analyses on the mitochondrial cytochrome oxidase I gene (mtCO1) revealed MEAM1, MED, and indigenous genetic groups in 53, 19, and 28%, respectively, of B. tabaci on tomatoes. Individual whiteflies were examined for endosymbionts through specific primers and phylogenetic analyses on the 16S rRNA and the 23S rRNA genes. Incidence of Portiera, Hamiltonella, Rickettsia, Wolbachia, and Cardinium was 81, 46, 28, 9, and 13%, respectively, in the B. tabaci samples analyzed. A new bacterium-endosymbiont in the genus Orientia was found and described in 63% of the samples of B. tabaci. A mixture of the endosymbionts Rickettsia+UAS_511+Cardinium was observed in 10% of samples of B. tabaci MED, while in the MEAM1 biotype, a combination of Hamiltonella+UAS_511 was in 32% of the samples analyzed. The study highlights the diversity and distribution of B. tabaci and its endosymbionts throughout the Culiacan Province and provides evidence for control of the insect pest. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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