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Your search keyword '"Germanò, M. P."' showing total 2 results
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2 results on '"Germanò, M. P."'

1. Chemical Composition of Juniperus phoenicea and J. drupacea Essential Oils and their Biological Effects in the Choriallantoic Membrane (CAM) Assay

Author

Aikaterini Koutsaviti, Tzakou, O., Galati, E. M., Certo, G., and Germanò, M. P.

Subjects
Plant Leaves, Biological Products, Juniperus, Juniperus phoenicea, Juniperus drupacea, Essential oil, GC analysis, Angiogenesis, CAM assay, Oils, Volatile, Animals, Biological Assay, Chick Embryo, Chorioallantoic Membrane
Abstract

The aim of the present study was the chemical analysis of the essential oils from Juniperus phoenicea and J. drupacea female cones and evaluation of their biological effects. Fresh samples, collected in Greece, were subjected separately to hydrodistillation and the oils obtained analyzed by GC-FID and GC-MS. The oils Were assessed using the CAM (chorioallantoic membrane) assay to evaluate their anti-angiogenic potential and the lack of irritant effects in topical application. GC analysis showed that mainly quantitative differences among the samples were observed: limonene was the most abundant compound in J. drupacea (27.0%) compared with J. phoenicea oil (1.6%); the content of a-pinene was high in both essential oils (J. phoenicea 22.1%, J. drpacea 26.1%) followed by germacrene D (J. phoenicea 7.4%, J. drpacea 7.1%, respectively). Nevertheless, qualitative differences were also detected as the diterpene 4-epi-abietal was present in a considerable amount (13.2%) in J. phoenicea essential oil, but was not detected in J. drupacea oil. In the CAM assay, only J. phoenicea. essential oil evidenced a rather weak anti-angiogenic activity compared with the standard retinoic acid, but no irritant effect was observed for either essential oil suggesting their safety for topical application.

Published
2018

2. Polyphenolic profile and biological activities of black carrot crude extract (Daucus carota L. ssp. sativus var. atrorubens Alef.).

Author

Smeriglio A, Denaro M, Barreca D, D'Angelo V, Germanò MP, and Trombetta D

Subjects
Angiogenesis Inhibitors analysis, Animals, Cells, Cultured, Chick Embryo, Flavonoids analysis, Free Radical Scavengers analysis, Humans, Proanthocyanidins analysis, Daucus carota chemistry, Plant Extracts chemistry, Polyphenols analysis
Abstract

Black carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) is a valuable source of carbohydrates, minerals and vitamins and contains also high amounts of anthocyanins giving the characteristic deep-purple color. These latter compounds are known as natural dyes used in the food and pharmaceutical industry that have recently attracted much attention for their healthful properties. The aim of this work was to investigate for the first time the polyphenolic profile and biological properties of a black carrot crude extract (BCCE) through an in-depth analysis of the main polyphenolic classes evaluating its antioxidant, cytoprotective and anti-angiogenic properties. Twenty five polyphenols were quantified by LC-DAD-FLD-MS/MS analysis (anthocyanins 78.06%, phenolic acids 17.89% and other flavonoids 4.06%) with polyglycosylated cyanidins as major components. In addition, BCCE showed a strong antioxidant and free radical scavenging activity particularly in the hydrogen transfer-based assays (ORAC and β-carotene bleaching) and a significant increase in the cell viability. Furthermore, BCCE exhibited a strong anti-angiogenic activity at the highest concentration assayed on the chick chorioallantoic membrane (50μg/egg). In conclusion, the obtained results demonstrated the antioxidant, cytoprotective and anti-angiogenic properties of BCCE, which highlight that the higher biological activity of BCCE is probably due to the synergic effects exerted by various polyphenolic classes., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Published
2018
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