1. Evaluating the pro-survival potential of apoptotic bodies derived from 2D- and 3D- cultured adipose stem cells in ischaemic flaps
- Author
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Gaoxiang Yu, Jian Ding, Ningning Yang, Lu Ge, Nuo Chen, Xuzi Zhang, Qiuchen Wang, Xian Liu, Xuanlong Zhang, Xiaoqiong Jiang, Yibo Geng, Chenxi Zhang, Jiadong Pan, Xiangyang Wang, Weiyang Gao, Zhijie Li, Hongyu Zhang, Wenfei Ni, Jian Xiao, Kailiang Zhou, and Liangliang Yang
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Adipose-derived mesenchymal stem cells ,3D cell culture ,Apoptotic bodies ,MicroRNAs ,Ischaemic flap ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract In the realm of large-area trauma flap transplantation, averting ischaemic necrosis emerges as a pivotal concern. Several key mechanisms, including the promotion of angiogenesis, the inhibition of oxidative stress, the suppression of cell death, and the mitigation of inflammation, are crucial for enhancing skin flap survival. Apoptotic bodies (ABs), arising from cell apoptosis, have recently emerged as significant contributors to these functions. This study engineered three-dimensional (3D)-ABs using tissue-like mouse adipose-derived stem cells (mADSCs) cultured in a 3D environment to compare their superior biological effects against 2D-ABs in bolstering skin flap survival. The findings reveal that 3D-ABs (85.74 ± 4.51) % outperform 2D-ABs (76.48 ± 5.04) % in enhancing the survival rate of ischaemic skin flaps (60.45 ± 8.95) % (all p
- Published
- 2024
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