1. Humoral immune response and coated or uncoated oxygenators during cardiopulmonary bypass surgery
- Author
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Gokhan Ilhan, Ayşe Gül Kunt, Erol Şener, Hakan Karamustafa, Şaban Ergene, Mustafa F. Sargon, Sedat Ozan Karakişi, Ugur Musabak, İdil Çankaya, Mustafa Kocakulak, Sahin Bozok, Nebiye Tüfekçi, RTEÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Karakişi, Sedat Ozan, Bozok, Şahin, Ergene, Şaban, İlhan, Gökhan, Karamustafa, Hakan, and Tüfekçi, Nebiye
- Subjects
Male ,Turkey ,law.invention ,Coronary artery bypass surgery ,Coated Materials, Biocompatible ,law ,Medicine ,Coronary Artery Bypass ,Cardiopulmonary Bypass ,biology ,Cardiopulmonary bypass ,Complement C4 ,General Medicine ,Equipment Design ,Middle Aged ,Flow Cytometry ,Blood proteins ,oxygenator ,Treatment Outcome ,Anesthesia ,Complement C3c ,Female ,Cardiology and Cardiovascular Medicine ,medicine.medical_specialty ,Oxygenator ,Oxygenators ,Surface Properties ,Phosphorylcholine ,Serum albumin ,Serum Albumin, Human ,Humoral inflammation ,Humans ,Serum Albumin ,Aged ,Oxygenators, Membrane ,business.industry ,Cardiovascular Topics ,Albumin ,humoral inflammation ,Surgery ,Immunity, Humoral ,Microscopy, Electron ,Cross-Sectional Studies ,Immunoglobulin M ,Immunoglobulin G ,biology.protein ,Adsorption ,business ,Biomarkers - Abstract
Bozok, Sahin/0000-0002-1256-5055; Musabak, Ugur/0000-0003-1511-7634; Sargon, Mustafa Fevzi/0000-0001-6360-6008 WOS: 000384800700018 PubMed: 27841912 Aim: To investigate and compare uncoated and phosphoryl-choline-coated oxygenators in terms of induction of humoral immune response during coronary artery bypass surgery. Methods: A total of 20 consecutive patients who underwent coronary artery bypass surgery were randomly distributed into two groups according to the type of oxygenator used during surgery. Group 1 consisted of 10 patients who were operated on using phosphorylcholine-coated oxygenators. Group 2 contained 10 patients who underwent surgery using uncoated oxygenators. Blood and oxygenator fibre samples were obtained and compared in terms of immunoglobulins (IgG, IgM), complements (C3c, C4), serum total protein and albumin levels using electron microscopy and flow cytometry. Results: in group 1, levels of IgM, IgG, total protein and serum albumin were significantly increased at the end of cardiopulmonary bypass (CPB) compared to those at the beginning of CPB. in group 2, C3c and C4 levels at the beginning of CPB were found to be significantly higher than at the end. Electron microscopic examination of oxygenator fibres demonstrated that phosphorylcholine-coated fibres were less likely to be adsorbed by serum proteins and complements than the uncoated fibres. Conclusion: Our results indicate that phosphorylcholine-coated oxygenators seemed to induce humoral immune response to a lesser extent than uncoated oxygenators during coronary artery bypass procedures.
- Published
- 2016