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Your search keyword '"Ilaria Sbarsi"' showing total 3 results
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3 results on '"Ilaria Sbarsi"'

1. Cytological, molecular, cytogenetic, and physiological characterization of a novel immortalized human enteric glial cell line

Author

Lisa Zanoletti, Aurora Valdata, Kristina Nehlsen, Pawan Faris, Claudio Casali, Rosalia Cacciatore, Ilaria Sbarsi, Francesca Carriero, Davide Arfini, Lies van Baarle, Veronica De Simone, Giulia Barbieri, Elena Raimondi, Tobias May, Francesco Moccia, Mauro Bozzola, Gianluca Matteoli, Sergio Comincini, and Federico Manai

Subjects
enteric glial cells, enteric nervous system, transgene immortalization, viral transduction, immortalized human cell line, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Enteric glial cells (EGCs), the major components of the enteric nervous system (ENS), are implicated in the maintenance of gut homeostasis, thereby leading to severe pathological conditions when impaired. However, due to technical difficulties associated with EGCs isolation and cell culture maintenance that results in a lack of valuable in vitro models, their roles in physiological and pathological contexts have been poorly investigated so far. To this aim, we developed for the first time, a human immortalized EGC line (referred as ClK clone) through a validated lentiviral transgene protocol. As a result, ClK phenotypic glial features were confirmed by morphological and molecular evaluations, also providing the consensus karyotype and finely mapping the chromosomal rearrangements as well as HLA-related genotypes. Lastly, we investigated the ATP- and acetylcholine, serotonin and glutamate neurotransmitters mediated intracellular Ca2+ signaling activation and the response of EGCs markers (GFAP, SOX10, S100β, PLP1, and CCL2) upon inflammatory stimuli, further confirming the glial nature of the analyzed cells. Overall, this contribution provided a novel potential in vitro tool to finely characterize the EGCs behavior under physiological and pathological conditions in humans.

Published
2023
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2. Luminex® xMAP® technology is an effective strategy for high-definition human leukocyte antigen typing of cord blood units prior to listing

Author

M. Guarene, C. Badulli, Carmine Tinelli, Rosalia Cacciatore, Anna Luisa Cremaschi, Annamaria Pasi, Paola Bergamaschi, Ilaria Sbarsi, and Cesare Perotti

Subjects
Oncology, medicine.medical_specialty, business.industry, Donor selection, Human leukocyte antigen typing, Biomedical Engineering, Medicine (miscellaneous), Bioengineering, General Medicine, Human leukocyte antigen, Luminex xmap, Biomaterials, 03 medical and health sciences, 0302 clinical medicine, 030220 oncology & carcinogenesis, Cord blood, Internal medicine, medicine, High definition, Typing, business, Cord blood transplantation, 030215 immunology
Abstract

Introduction: Allele-level donor–recipient match at HLA-A, HLA-B, HLA-C and HLA-DRB1 loci impacts the outcome after cord blood transplantation for hematologic malignancies and modifies the strategy of donor selection. High definition of both class I and II HLA loci at time of listing is a way to improve the attractiveness of cord blood bank inventories, reducing the time for donor search and procurement and simplifying donor choice, in particular, for patients of non-European heritage. Methods: In 2014, Luminex® xMAP® technology was introduced in our laboratory practice and was applied to cord blood units typing. In this study, we evaluated the impact of this strategy in comparison with the platform in use until 2013, relying on LiPA reverse polymerase chain reaction–sequence-specific oligonucleotide (revPCR-SSO) plus polymerase chain reaction–sequence-specific primer (PCR-SSP). Results: In 2014, the time for testing was shorter (141 vs 181 days on average), the number of test repetitions was lower (in particular for HLA-A locus, p = 0.026), and the cost reduced (240.7 vs 395.6 euros per unit on average) compared to 2013, demonstrating that Luminex xMAP technology is superior to the previous approach. Conclusion: Luminex xMAP platform has useful application in cord blood banking programs, to achieve high-definition HLA typing of cord blood units at the time of banking in a quick, accurate, and cost-effective manner.

Published
2018
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3. Luminex

Author

Marco, Guarene, Carla, Badulli, Anna L, Cremaschi, Ilaria, Sbarsi, Rosalia, Cacciatore, Carmine, Tinelli, Annamaria, Pasi, Paola, Bergamaschi, and Cesare G, Perotti

Subjects
Histocompatibility Testing, Humans, Fetal Blood, Polymerase Chain Reaction
Abstract

Allele-level donor-recipient match at HLA-A, HLA-B, HLA-C and HLA-DRB1 loci impacts the outcome after cord blood transplantation for hematologic malignancies and modifies the strategy of donor selection. High definition of both class I and II HLA loci at time of listing is a way to improve the attractiveness of cord blood bank inventories, reducing the time for donor search and procurement and simplifying donor choice, in particular, for patients of non-European heritage.In 2014, LuminexIn 2014, the time for testing was shorter (141 vs 181 days on average), the number of test repetitions was lower (in particular for HLA-A locus, p = 0.026), and the cost reduced (240.7 vs 395.6 euros per unit on average) compared to 2013, demonstrating that Luminex xMAP technology is superior to the previous approach.Luminex xMAP platform has useful application in cord blood banking programs, to achieve high-definition HLA typing of cord blood units at the time of banking in a quick, accurate, and cost-effective manner.

Published
2018

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