91 results on '"J., Beneš"'
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2. Dávkování enoxaparinu a výskyt žilního trombembolismu u kriticky nemocných pacientů s covidem-19 – retrospektivní observační studie.
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J., Beneš, M., Kalina, and V., Černý
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VENOUS thrombosis , *COVID-19 , *LOW-molecular-weight heparin , *INTENSIVE care units , *PULMONARY embolism - Abstract
Objective: COVID-19 is associated with a high risk of thromboembolic disease (VTE) and this risk is further increased in critically ill patients. Elevated D-dimer levels are a predictor of severe COVID-19 disease. Data on the incidence of VTE, mortality of critically ill patients with COVID-19, and its association with the dose of prophylactic anticoagulation in Czechia have not been published. We performed a retrospective analysis of patients with COVID-19 admitted to the intensive care unit. The primary endpoint was new-onset deep vein thrombosis during ICU stay. Secondary objectives were incidence of pulmonary embolism, venous thromboembolism (deep vein thrombosis and/or pulmonary embolism), number of episodes of major bleeding, ICU length of stay, ICU lethality, 28-day, 90-day, and 180-day lethality, and duration of artificial pulmonary ventilation. Design: retrospective observational study. Setting: Intensive care units of a large general hospital. Material and methods: We performed a retrospective analysis of medical records of patients hospitalized for COVID-19 in 4 intensive care units of a large general hospital between September 2020 and April 2021. Patients meeting the following inclusion criteria were included for analysis: age at least 18 years, principal diagnosis of COVID-19, length of stay in the ICU at least 72 hours, and absence of VTE on ICU admission. Results: A total of 44 cases (13.3%) of new-onset VTE were identified. A total of 9 patients were diagnosed with pulmonary embolism, 31 patients with deep vein thrombosis, and 4 patients with deep vein thrombosis concomitant with pulmonary embolism. 274 patients received a standard prophylactic dose of enoxaparin, and 56 patients received intermediate or therapeutic doses. ICU, 28-day, 90-day, and 180-day mortality rates were 27%, 32%, 44%, and 47%, respectively. Mortality was not significantly associated with anticoagulant dose. There was a significant association between D-dimer levels and mortality. Conclusion: The incidence of VTE found in a cohort of critically ill patients with COVID-19 was high. Elevated D-dimer levels were associated with 30-day, 90-day, and 180-day mortality. Increased dosing of low-molecular-weight heparin was not associated with a lower incidence of thromboembolism or lower mortality and cannot be recommended. [ABSTRACT FROM AUTHOR] more...
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- 2024
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3. Year 2022 in Review - Intensive care medicine - Cardiovascular and general issues
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J Kletečka, J Zatloukal, O Smékalová, and J Beneš
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Anesthesiology and Pain Medicine ,Critical Care and Intensive Care Medicine - Published
- 2022
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4. Rok 2023 v přehledu - Intenzivní medicína - kardiovaskulární problematika.
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J., Beneš, J., Kletečka, O., Smékalová, and J., Zatloukal
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INFUSION therapy , *CARDIOVASCULAR system , *CRITICAL care medicine , *HEART failure - Abstract
This review article offers a summary of the most essential newly published findings in the field of cardiovascular issues in acute conditions published in 2023. At the same time, we try to place these new findings in the context of previous knowledge to avoid an unnecessary pendulum effect. This publication does not intend to alter our daily approach; instead, it provides readers with an overview of noteworthy information that could contribute to future care considerations. [ABSTRACT FROM AUTHOR] more...
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- 2023
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5. Bartonella endocarditis as a cause of acute heart failure. Importance of routine echocardiographic examination in acute respiratory failure in intensive care units
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J Beneš, J Široký, J Lhotský, and J Zatloukal
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Anesthesiology and Pain Medicine ,Critical Care and Intensive Care Medicine - Abstract
Bartonella quintana byla v sirsim povědomi lekařske veřejnosti předevsim pocatkem minuleho stoleti v souvislosti s výskytem tzv. zakopove horecky. V dnesni době se jedna o ne přilis casteho původce infekcni endokarditidy (IE) předevsim u pacientů se sniženou funkci imunitniho systemu. Zaludnosti teto bakterie je, že ve větsině připadů neni detekovatelna v hemokultuře, nebo až po velmi dlouhe kultivaci. Definitivni diagnozu se větsinou podaři urcit až na zakladě polymerazove řetězove reakce (PCR) z odebraneho chlopenniho materialu. Kazuistika prezentuje připad 69lete ženy přijate do intenzivni pece původně pro bronchopneumonii, u ktere byla diky rutinně provedenemu vstupnimu echokardiografickemu vysetřeni diagnostikovana těžka chlopenni vada a nasledně bartonellova endokarditida. Předkladana kazuistika se snaži vyzdvihnout význam rutinniho echokardiografickeho vysetřeni provaděneho intenzivistou‑nekardiologem a taktež upozornit na ne přilis castý připad kultivacně negativni endokarditidy. more...
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- 2021
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6. Diagnostika COVID-19 pneumonie pomocí výpočetní tomografie, naše zkušenosti
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J Ferda, H Mírka, J Baxa, M Matějovič, and J Beneš
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Anesthesiology and Pain Medicine ,Critical Care and Intensive Care Medicine - Published
- 2020
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7. Use of albumin in perioperative and intensive care medicine
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D Astapenko, J Pouska, J Škola, J Beneš, J Náhlovský, M Mynář, Z Turek, and V Černý
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Anesthesiology and Pain Medicine ,Critical Care and Intensive Care Medicine - Abstract
Albumin je stale siroce studovaným proteinem lidskeho těla. Historicky nejdele je podavan jako objemova nahrada s využitim jeho onkotických vlastnosti. V oblasti tekutinove terapie je v poslednich letech dale srovnavan s ostatnimi typy roztoků v různých klinických indikacich a je sledovana zejmena jeho bezpecnost. Předmětem zajmu výzkumu jsou jeho dalsi biologicke ucinky, ktere jsou využivany ve specifických klinických situacich: jaterni selhani, prevence selhani ledvin, syndrom akutni respiracni tisně dospělých, traumaticke poskozeni mozku, ovarialni hyperstimulacni syndrom. Přehledový clanek shrnuje soucasný stav odborneho poznani a jeho využiti jak u dospělých pacientů v intenzivni peci, tak v neonatologii. Diskutuje vybrane klinicke syndromy a dosud znamou fyziologii albuminu v lidskem těle. Věnuje se tež použiti albuminu v postupech mimotělni podpory organových funkci: mimotělni oběh v kardiochirurgii, dialýza a plazmafereza. more...
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- 2020
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8. Cílená regulace tělesné teploty: konsenzuální stanovisko mezioborové pracovní skupiny
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M Balík, J Bělohlávek, J Beneš, V Černý, K Cvachovec, T Drábek, P Ošťádal, R Škulec, and J Škola
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Anesthesiology and Pain Medicine ,Critical Care and Intensive Care Medicine - Published
- 2020
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9. Pulmonary alveolar proteinosis: repeated bilateral whole lung lavage supported by extracorporeal membrane oxygenation
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J Beneš, P Roleček, and V Černý
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Anesthesiology and Pain Medicine ,business.industry ,medicine.medical_treatment ,medicine ,Extracorporeal membrane oxygenation ,Whole lung lavage ,Critical Care and Intensive Care Medicine ,Pulmonary alveolar proteinosis ,medicine.disease ,Nuclear medicine ,business - Abstract
Celoplicni lavaž velkým množstvim fyziologickeho roztoku je v soucasnosti zlatým standardem lecby plicni alveolarni proteinozy. Toto vzacne onemocněni charakterizovane akumulaci lipoproteinozniho materialu v alveolech vede k respiracnimu selhani různe tiže. V některých připadech neni možne provest celoplicni lavaž vyžadujici jednostrannou plicni ventilaci bez rizika zavažne periproceduralni hypoxie. V těchto vzacných připadech lze výkon provest za podpory mimotělni membranove oxygenace. Předkladana kazuistika popisuje připad 41leteho pacienta uspěsně leceneho opakovanou oboustrannou celoplicni lavaži provedenou za podpory veno‑venozni mimotělni membranove oxygenace. Mimotělni membranova oxygenace je použivana k provedeni celoplicni lavaže u pacientů s rizikem život ohrožujici hypoxie. Zvysujici se dostupnost a bezpecnost metod mimotělni podpory životnich funkci umožňuje rozsiřit indikaci jejich použiti o připady, kdy by provedeni plicni lavaže za použiti jednostranne plicni ventilace bylo sice možne, nicmeně zatižene významným rizikem komplikaci v důsledku zavažne hypoxie. Tato praxe zvysuje nejen bezpecnost, ale i efektivitu procedury, neboť umožnuje provest v jedne době oboustrannou lavaž. more...
- Published
- 2020
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10. Sedation in the intensive care unit - part I
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Pavel Suk, J Kletečka, Vladimír Šrámek, and J Beneš
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Anesthesiology and Pain Medicine ,law ,business.industry ,Anesthesia ,Sedation ,Medicine ,medicine.symptom ,Critical Care and Intensive Care Medicine ,business ,Intensive care unit ,law.invention - Abstract
Sedace je důležitou soucasti pece o ventilovane pacienty, jejimž cilem je zlepsit jejich komfort. V tomto přehledovem clanku je diskutovana adekvatni analgezie, klinicke hodnoceni hloubky sedace, srovnani lehke a hluboke sedace vcetně vlivu na klinicky relevantni výsledky a srovnani nejběžnějsich sedativ. more...
- Published
- 2020
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11. Hyperbaric oxygen enhances collagen III formation in wound of ZDF rat
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J, Růžička, M, Grajciarová, L, Vištejnová, P, Klein, F, Tichánek, Z, Tonar, J, Dejmek, J, Beneš, L, Bolek, R, Bajgar, and J, Kuncová
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Male ,Hyperbaric Oxygenation ,Random Allocation ,Wound Healing ,Collagen Type III ,Animals ,Articles ,Diabetic Foot ,Rats, Zucker - Abstract
Diabetic foot ulcer (DFU) is a serious complication of diabetes and hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. The evidence supporting the use of HBOT in DFU treatment is controversial. The aim of this work was to introduce a DFU model in ZDF rat by creating a wound on the back of an animal and to investigate the effect of HBOT on the defect by macroscopic evaluation, quantitative histological evaluation of collagen (types I and III), evaluation of angiogenesis and determination of interleukin 6 (IL6) levels in the plasma. The study included 10 rats in the control group (CONT) and 10 in the HBOT group, who underwent HBOT in standard clinical regimen. Histological evaluation was performed on the 18th day after induction of defect. The results show that HBOT did not affect the macroscopic size of the defect nor IL6 plasma levels. A volume fraction of type I collagen was slightly increased by HBOT without reaching statistical significance (1.35+/-0.49 and 1.94+/-0.67 %, CONT and HBOT, respectively). In contrast, the collagen type III volume fraction was ~120 % higher in HBOT wounds (1.41+/-0.81 %) than in CONT ones (0.63+/-0.37 %; p=0.046). In addition, the ratio of the volume fraction of both collagens in the wound ((I+III)w) to the volume fraction of both collagens in the adjacent healthy skin ((I+III)h) was ~65 % higher in rats subjected to HBOT (8.9+/-3.07 vs. 5.38+/-1.86 %, HBOT and CONT, respectively; p=0.028). Vessels density (number per 1 mm2) was found to be higher in CONT vs. HBOT (206.5+/-41.8 and 124+/-28.2, respectively, p0.001). Our study suggests that HBOT promotes collagen III formation and decreases the number of newly formed vessels at the early phases of healing. more...
- Published
- 2021
12. Negative pressure pulmonary edema
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J. Kletečka, K. Hadrabová, and J. Beneš
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medicine.medical_specialty ,Anesthesiology and Pain Medicine ,business.industry ,Negative pressure pulmonary edema ,Internal medicine ,Cardiology ,medicine ,Critical Care and Intensive Care Medicine ,business - Published
- 2019
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13. Online resources in intensive care medicine
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J. Beneš and J. Kletečka
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Anesthesiology and Pain Medicine ,business.industry ,medicine ,Medical emergency ,Critical Care and Intensive Care Medicine ,medicine.disease ,business - Published
- 2019
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14. Repeatedly negative PCR results in patients with COVID-19 symptoms: Do they have SARS-CoV-2 infection or not?
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J, Beneš, O, Džupová, A, Poláková, and N, Sojková
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Adult ,Aged, 80 and over ,Male ,Young Adult ,SARS-CoV-2 ,COVID-19 ,Humans ,Female ,Middle Aged ,Coronavirus Infections ,Pandemics ,Polymerase Chain Reaction ,Aged - Abstract
To point out possible infection with SARS-CoV-2 in symptomatic patients despite repeated negative nasopharyngeal swab tests for SARS-CoV-2.A retrospective observational study carried out at the Na Bulovce Hospital from the beginning of the pandemic until November 2020 included patients (1) who had symptoms compatible with COVID-19; (2) whose nasopharyngeal swab PCR tests in the presence of acute respiratory infection symptoms yielded two consecutive negative results; (3) in whom SARS-CoV-2 infection was subsequently confirmed by serology. Basic demographic and epidemiological data, symptoms, laboratory test results, X-ray findings and timing of virological tests were analysed for these patients.Seventeen patients met the inclusion criteria, 14 men and three women, aged 19-84 years with a median of 59 years, of whom 14 were hospitalized and three were treated as outpatients. Only seven patients were aware of the previous contact with an infected person. The main symptoms were fever, cough, headache, weakness, fatigue and shortness of breath. Pneumonia was found in 12 patients, four of whom developed respiratory insufficiency requiring ventilatory support. Most patients showed a uniform combination of haematological, biochemical and radio-logical findings: absence of eosinophils and increased polymorphonuclear/lymphocyte ratio; elevation of serum lactate dehydrogenase; elevation of CRP without rise of procalcitonin; typical chest CT or X-ray findings. All patients recovered. Coronavirus antigen test was performed in six patients, with all of them testing negative. SARS-CoV-2 infection was confirmed serologically by the detection of specific IgG and IgA in all 17 patients and also IgM in six patients, not before day 8 of the onset of symptoms.Our study showed that some patients with acute COVID-19 may test repeatedly negative by nasopharyngeal swab PCR. These cases should be interpreted as a low viral load in the upper respiratory tract rather than false negativity of PCR. Such alternative is not envisaged in the algorithms used. Considering our results, the following recommendation can be made: If, despite negative PCR tests, COVID-19 is still suspected based on clinical symptoms and epidemiological evidence, preliminary diagnosis can be made on the basis of comprehensive assessment of the laboratory test and X-ray findings. Final confirmation of the aetiology relies on serological tests performed two weeks after the onset of symptoms. more...
- Published
- 2021
15. Air flight barotrauma and meningitis: causality seems real
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O Džupová and J Beneš
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medicine.medical_specialty ,business.industry ,General Medicine ,medicine.disease ,Causality ,Acute bacterial meningitis ,Otitis ,Paranasal sinuses ,medicine.anatomical_structure ,Otorhinolaryngology ,medicine ,Bacterial meningitis ,medicine.symptom ,Sinusitis ,Intensive care medicine ,business ,Meningitis ,Air travel - Abstract
BackgroundBarotrauma to the middle-ear cavity and paranasal sinuses is a relatively common flight-related health problem. Occasionally, it may result in severe mechanical or infectious intracranial complications; these have been rarely reported to date.ObjectiveFour cases of acute bacterial meningitis following air travel are presented, and its pathogenesis is briefly described.ConclusionNeurological symptoms occurring after air flight should prompt proper investigation. Otitis media and sinusitis are common primary focuses of bacterial meningitis. Severe complications of air flight barotrauma may be underreported. more...
- Published
- 2020
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16. Pelvic actinomycosis and IUD
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B, Sehnal, J, Beneš, Z, Kolářová, M, Mojhová, and M, Zikán
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Vaginal Smears ,Carrier State ,Actinomyces ,Humans ,Female ,Cervix Uteri ,Actinomycosis ,Intrauterine Devices - Abstract
To summarize knowledge about the mana-gement in women with proven actinomyces in uterine cervix and inserted intrauterine device (IUD).An overall review.Actinomycosis is an uncommon but important subacute or chronic infection caused by anaerobic or microaerophilic bacteria, mainly within the Actinomyces genus. Actinomycosis can affect various organs and tissues in the human body, often manifesting draining sinuses, abscess formation and fibrosis. The pelvic form in women is the most common in the developed countries. Long-duration treatment with antibiotics can be completely effective even in cases of heavy disease. Although pelvic actinomycosis is predominantly associated with the longstanding use of intrauterine device, the risk of future symptomatic infection is extremly low even in women with a cervical Pap smear positive for actinomyces-like organisms (ALO). Therefore the identification of actinomycetes by cytology after cervical Pap smears is not diagnostic nor predictive of any disease because the actinomycetes normally reside in the female genital tract. In the absence of symptoms, patients with ALO on a Pap test do not need antimicrobial treatment or IUD removal. Nevertheless, women choosing an IUD for contraception should know that there is very low risk of developing the infection in later years after insertion.The sources of literature conclude that removal of the intrauterine device in a patients with a positive ALO in the uterine cervix is not necessary and antibiotics treatment is not required. However, IUD must be changed at least every five years in order to limit the risk of the development of pelvic actinomycosis. more...
- Published
- 2019
17. Effect of O-methyl-β-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of l-glutamate in brain nerve terminals
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Daniel Horák, Miroslava Trchová, Konstantin Paliienko, Zuzana Procházková, Tatiana Borisova, Arsenii Borysov, Milan J. Beneš, and Artem Pastukhov
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Male ,0301 basic medicine ,Stereochemistry ,Presynaptic Terminals ,Glutamic Acid ,Ferric Compounds ,Membrane Potentials ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Colloid and Surface Chemistry ,Extracellular ,Animals ,Carbon Radioisotopes ,Rats, Wistar ,Physical and Theoretical Chemistry ,Magnetite Nanoparticles ,HEPES ,chemistry.chemical_classification ,Cyclodextrin ,Cell Membrane ,beta-Cyclodextrins ,Glutamate receptor ,Brain ,nutritional and metabolic diseases ,Biological Transport ,Surfaces and Interfaces ,General Medicine ,Silanes ,eye diseases ,Rats ,Kinetics ,Cholesterol ,030104 developmental biology ,Membrane ,chemistry ,Silanization ,Biophysics ,Magnetic nanoparticles ,030217 neurology & neurosurgery ,Synaptosomes ,Biotechnology ,Superparamagnetism - Abstract
Changes in cholesterol concentration in the plasma membrane of presynaptic nerve terminals nonspecifically modulate glutamate transport and homeostasis in the central nervous system. Reduction of the cholesterol content in isolated rat brain nerve terminals (synaptosomes) using cholesterol-depleting agents decreases the glutamate uptake and increases the extracellular level of glutamate in nerve terminals. Extraction of cholesterol from the plasma membrane and its further removal from the synaptosomes by external magnetic field can be achieved by means of magnetic nanoparticles with immobilized cholesterol-depleting agent such as O -methyl-β-cyclodextrin (MCD). A simple approach is developed for preparation of maghemite (γ-Fe 2 O 3 ) nanoparticles containing chemically bonded MCD. The method is based on preparation of a silanization agent containing MCD. It is synthesized by the reaction of triethoxy(3-isocyanatopropyl)silane with MCD. Base-catalyzed silanization of superparamagnetic γ-Fe 2 O 3 provides a relatively stable colloid product containing 48 μmol of MCD g −1 . MCD-modified γ-Fe 2 O 3 nanoparticles decrease the initial rate of the uptake and accumulation of l -[ 14 C]glutamate and increase the extracellular l -[ 14 C]glutamate level in the preparation of nerve terminals. The effect of MCD-immobilized nanoparticles is the same as that of MCD solution; moreover, magnetic manipulation of the nanoparticles enables removal of bonded cholesterol. more...
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- 2017
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18. První roky Fondu mobility SMAI ČSARIM.
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T., Bönischová, M., Klincová, P., Štourač, and J., Beneš
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- 2024
19. Na ZOO nebo na ARO? aneb aprílové jazykovědné okénko českých obrozenců.
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M., Kovář, O., Smékalová, V., Sochorová, J., Máca, and J., Beneš
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ZOOS - Published
- 2024
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20. Point of care vyšetření krevní srážlivosti - současné možnosti.
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J., Zatloukal, M., Horáková, L., Bultasová, J., Pouska, J., Kletečka, and J., Beneš
- Abstract
Copyright of Anaesthesiology & Intensive Medicine / Anesteziologie a Intenzivní Medicína is the property of Czech Medical Association of JE Purkyne and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2021
21. [Antibiotic treatment of clostridial colitis]
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J, Beneš and S, Polívková
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Aminoglycosides ,Clostridioides difficile ,Recurrence ,Vancomycin ,Metronidazole ,Drug Resistance, Bacterial ,Humans ,Enterocolitis, Pseudomembranous ,Fidaxomicin ,Anti-Bacterial Agents - Abstract
The advantages and disadvantages of various antibiotics used in the treatment of Clostridium difficile infection (CDI) are compared with respect to their pharmacokinetic and pharmacodynamic properties. Recommendations are made for their optimal use in clinical practice. Metronidazole is suitable for the treatment of mild forms of CDI which are essentially self-limiting. Vancomycin kills clostridia reliably but the treatment is encumbered with considerable risk of recurrence. This can be decreased by shortening the treatment to seven days and then switching to a (pulse, taper, chaser) regimen to prevent recurrence or by active restoration of the intestinal ecosystem (fecal transplant). Fidaxomicin works faster than vancomycin and is associated with a lower risk of recurrence. Thus, it can be profitably used in patients with impending ileus and also in those whose medical condition does not allow prolonged treatment. The duration of fidaxomicin treatment could be reduced to as few as five days. Rifaximin does not have a clear place in the treatment of CDI because no compelling data are available on its efficacy in this disease. The risk of resistance is also important. Tigecycline is a promising antibiotic for parenteral use. According to the available data, it should be more effective than intravenous metronidazole which has been considered the drug of choice.Clostridial colitis is associated with intestinal dysmicrobia which is the major cause of recurrence. Severe dysmicrobia cannot be treated by antibiotics but only by gut flora restoration; stool transplant from a healthy donor is the only proven therapy for this condition. more...
- Published
- 2016
22. [Pyoderma Gangrenosum Mimicking Necrotising Fasciitis after Rotator Cuff Reconstruction]
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J, Včelák, R, Šuman, and J, Beneš
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Diagnosis, Differential ,Male ,Treatment Outcome ,Adrenal Cortex Hormones ,Humans ,Fasciitis, Necrotizing ,Plastic Surgery Procedures ,Pyoderma Gangrenosum ,Aged ,Rotator Cuff Injuries - Abstract
Gangrenous pyoderma is a rare complication of surgical treatment mimicking bacterial necrotizing fasciitis. The characteristic symptoms include a pre-existing immune disorder, negative results of the relevant microbiological examinations, no response to any antibiotic therapy, and deteriorating of the condition after radical debridement. Another typical feature is a discrepancy between an ominous appearance of the wound accompanied by leucocytosis and an elevated CRP level, and the patient s good general condition with low-grade fever and no signs of sepsis. The case of a 68-year-old man who underwent an elective open reconstruction of rotator cuff tears of the left shoulder is presented. The surgery was complicated with pyoderma gangrenosum that was first treated unsuccessfully by repeated revision surgery and broad-spectrum antibiotic therapy. The patient was ultimately cured with corticosteroids. The sequellae included limited motion of the shoulder joint and extensive destruction and scarring of the adjacent soft tissue.gangrenous pyoderma, necrotizing fasciitis, corticosteroids. more...
- Published
- 2016
23. Kognitivní poruchy v perioperační a intenzivní péči.
- Author
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J., Kletečka, K., Soumar, and J., Beneš
- Abstract
Copyright of Anaesthesiology & Intensive Medicine / Anesteziologie a Intenzivní Medicína is the property of Czech Medical Association of JE Purkyne and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2017
24. Endoteliální glykokalyx a tekutinová terapie v intenzivní a perioperační medicíně.
- Author
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D., Astapenko, J., Pouska, V., Černý, and J., Beneš
- Abstract
Copyright of Anaesthesiology & Intensive Medicine / Anesteziologie a Intenzivní Medicína is the property of Czech Medical Association of JE Purkyne and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2017
25. Predikce odpovědi na podání tekutiny - tekutinová reaktivita.
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J., Beneš
- Abstract
Assessment of fluid responsiveness is a very important component of fluid therapy in the anaesthetized and critically ill patients. Using a broad spectrum of different tests allows the assessment of the impact of preload change on the central hemodynamics prior to the actual fluid infusion, which may help deliver individiual patient-centered fluid therapy and decrease the risks associated with fluid overload. This review article is a printed supplement and explanation of the electronic Smart Card issued for better understanding of the issue of fluid responsiveness. [ABSTRACT FROM AUTHOR] more...
- Published
- 2017
26. Tekutinová terapie v intenzivní péči.
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J., Beneš
- Abstract
Fluid therapy is an everyday issue in the daily routine of every anaesthesiologist and intensive care physician. Like with any other drug, administration of intravenous fluids is coupled with effects wanted and unwanted. This review article is a printed supplement and explanation of the electronic Smart Card issued for better understanding of how and when fluids should be indicated and administered. [ABSTRACT FROM AUTHOR] more...
- Published
- 2016
27. Data v oboru - k čemu a komu mají sloužit?
- Author
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V., Černý and J., Beneš
- Published
- 2023
28. Intraperitoneal versus intravenous administration of Flamma®-conjugated PEG-alendronate-coated upconversion nanoparticles in a mouse pancreatic cancer model.
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Vasylyshyn T, Patsula V, Větvička D, Shapoval O, Pankrác J, Kabešová M, Beneš J, and Horák D
- Abstract
Pancreatic cancer is one of the most common forms of malignant disease with a poor survival prognosis. Currently, nanomedicine holds great promise for targeted diagnosis and treatment of this cancer, which also reduces toxic side effects. In this work, we prepared PEG-coated monodisperse upconversion nanoparticles (UCNPs) with a conjugated Flamma® fluorescent dye for imaging and detection of particle distribution in vivo . We performed a thorough physicochemical characterization of the particles and determined their colloidal and chemical stability in several aqueous media such as water, PBS, Dulbecco's modified Eagle's medium and artificial lysosomal fluid. Luminescence resonance energy transfer from the emission of UCNPs as a donor to the Flamma® as an acceptor was confirmed. Intraperitoneal versus intravenous administration was then compared in terms of biodistribution of particles in various organs in the orthotopic mice pancreatic cancer model. The intraperitoneal route was preferred over the intravenous one, because it significantly increased the accumulation of particles in the tumor tissue. These new UCNP@Ale-PEG-Flamma® nanoparticles are thus promising for new treatment avenues for pancreatic cancer., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.) more...
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- 2024
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29. Photon-counting CT using multi-material decomposition algorithm enables fat quantification in the presence of iron deposits.
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Hollý S, Chmelík M, Suchá S, Suchý T, Beneš J, Pátrovič L, and Juskanič D
- Subjects
- Magnetic Resonance Imaging methods, Phantoms, Imaging, Algorithms, Iron, Tomography, X-Ray Computed methods
- Abstract
Purpose: A new generation of CT detectors were recently developed with the ability to measure individual photon's energy and thus provide spectral information. The aim of this work was to assess the performance of simultaneous fat and iron quantification using a clinical photon-counting CT (PCCT) and its comparison to dual-energy CT (DECT), MRS and MRI at 3 T., Methods: Two 3D printed cylindrical phantoms with 32 samples (n = 12 fat fractions between 0 % and 100 %, n = 20 with mixtures of fat and iron) were scanned with PCCT and DECT scanners for comparison. A three-material decomposition approach was used to estimate the volume fractions of fat (FF), iron and soft tissue. The same phantoms were examined by MRI (6-echo DIXON, a.k.a. Q-DIXON) and MRS (multi-echo STEAM, a.k.a. HISTO) at 3 T for comparison., Results: PCCT, DECT, MRI and MRS computed FFs showed correlation with reference fat fraction values in samples with no iron (r > 0.98). PCCT decomposition showed slightly weaker correlation with FFref in samples with added iron (r = 0.586) compared to MRI (r = 0.673) and MRS (r = 0.716) methods. On the other hand, it showed no systematic over- or underestimation. Surprisingly, DECT decomposition-derived FF showed strongest correlation (r = 0.758) in these samples, however systematic overestimation was observed. FF values computed by three-material PCCT decomposition, DECT decomposition, MRI and MRS were unaffected by iron concentration., Conclusions: This in-vitro study shows for the first time that photon-counting computed tomography may be used for quantification of fat content in the presence of iron deposits., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Associazione Italiana di Fisica Medica e Sanitaria. Published by Elsevier Ltd. All rights reserved.) more...
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- 2024
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30. Intraoperative Measurement of Endotracheal Tube Cuff Pressure and Its Change During Surgery in Correlation With Recurrent Laryngeal Nerve Palsies, Hoarseness, and Dysphagia After Anterior Cervical Discectomy and Fusion: A Prospective Randomized Controlled Trial.
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Sejkorová A, Bolcha M, Beneš J, Kalhous J, Sameš M, and Vachata P
- Abstract
Study Design: Prospective randomized controlled trial., Objectives: Adjustment of endotracheal tube cuff pressure (ETCP) in anterior cervical discectomy and fusion (ACDF) may influence the incidence of complications such as recurrent laryngeal nerve palsy (RLNP), hoarseness, and dysphagia., Methods: The prospective randomized controlled trial was designed to investigate the influence of ETCP on the incidence of postoperative complications. All eligible patients underwent vocal cord examination before and after ACDF and were randomized into a control group (CG) and intervention group (IG). Endotracheal tube cuff pressure was passively monitored in CG, and in IG, it was maintained at 20 mmHg. Outcomes were evaluated during hospitalization and during follow-up., Results: A total of 98 patients were randomized, each group consisted of 49 patients. Statistical analysis showed that gender and age did not influence the incidence of complications. In CG, duration of retractor placement and extent of approach significantly impacted the occurrence of complications. The incidence of postoperative RLNP was 8.2% in IG and 12.2% in CG, hoarseness and dysphonia were present in 18.4% in IG and in 37.5% in CG, and dysphagia in 20.8% in IG and in 22.5% in CG. Hoarseness was significantly present more in CG ( P = .018). Only one patient from CG presented with RLNP after 1 year, the remaining nine patients spontaneously recovered., Conclusions: Unregulated ETCP can lead to a significantly higher incidence of hoarseness; however, its improvement rate is 100%. The early postoperative complication rate was higher in CG, and after one year, 1 patient had RLNP and 1 patient had dysphagia. more...
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- 2023
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31. A word on the microbiome: considerations about the history, current state, and terminology of an emerging discipline.
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Černý V, Hrdý J, Beneš J, and Tláskalová H
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- Humans, Microbiota genetics
- Abstract
Since the beginning of the 21st century, a new discipline, microbiome science, has emerged as a key part of microbiology and related biomedical and ecological sciences. Microbiome science uses highly advanced molecular genetic and bioinformatic methods to study complex microbial communities. Unlike isolated microbes, microbial communities shaped by the environment, referred to as microbial consortia or microbiomes, follow their own laws that allow for significant functional specialization. The synthesis of multimethodology and multidisciplinary data enables microbiome science to move towards a holistic picture of the microbiome in an exceptionally effective way, but on the other hand, it burdens the field with terminological ambiguity of the key terms, which consequently need to be clearly codified in accordance with the international trends in the use of technical nomenclature. To this end, we present in our article the official position of the Czech Microbiome Society of the J. E. Purkyně Czech Medical Society on the use of appropriate Czech terms in both professional and general communication. more...
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- 2023
32. The way to data: opinions and recommendations for the provision of health data for secondary use.
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Tichopád A, Augustynek M, Beneš J, Dlouhý M, Doležal T, Horáková D, Kršek M, Lhotska L, Panzner P, Penhaker M, Petr M, Piťha J, Popesko B, Rožánek M, Táborský M, and Vrablík M
- Subjects
- Humans, Privacy, Data Anonymization, Confidentiality
- Abstract
Healthcare data held by state-run organisations is a valuable intangible asset for society. Its use should be a priority for its administrators and the state. A completely paternalistic approach by administrators and the state is undesirable, however much it aims to protect the privacy rights of persons registered in databases. In line with European policies and the global trend, these measures should not outweigh the social benefit that arises from the analysis of these data if the technical possibilities exist to sufficiently protect the privacy rights of individuals. Czech society is having an intense discussion on the topic, but according to the authors, it is insufficiently based on facts and lacks clearly articulated opinions of the expert public. The aim of this article is to fill these gaps. Data anonymization techniques provide a solution to protect individuals' privacy rights while preserving the scientific value of the data. The risk of identifying individuals in anonymised data sets is scalable and can be minimised depending on the type and content of the data and its use by the specific applicant. Finding the optimal form and scope of deidentified data requires competence and knowledge on the part of both the applicant and the administrator. It is in the interest of the applicant, the administrator, as well as the protected persons in the databases that both parties show willingness and have the ability and expertise to communicate during the application and its processing. more...
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- 2023
33. Shock Index for Early Detection of Low Plasma Fibrinogen in Trauma: A Prospective Observational Cohort Pilot Study.
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Škola J, Bílská M, Horáková M, Tégl V, Beneš J, Škulec R, and Černý V
- Abstract
Shock index (a ratio between heart rate and systolic blood pressure) predicts transfusion requirements and the need for haemostatic resuscitation in severe trauma patients. In the present study, we aimed to determine whether prehospital and on-admission shock index values can be used to predict low plasma fibrinogen in trauma patients. Between January 2016 and February 2017, trauma patients admitted from the helicopter emergency medical service into two large trauma centres in the Czech Republic were prospectively assessed for demographic, laboratory and trauma-associated variables and shock index at scene, during transport and at admission to the emergency department. Hypofibrinogenemia defined as fibrinogen plasma level of 1.5 g·L
-l was deemed as a cut-off for further analysis. Three hundred and twenty-two patients were screened for eligibility. Of these, 264 (83%) were included for further analysis. The hypofibrinogenemia was predicted by the worst prehospital shock index with the area under the receiver operating characteristics curve (AUROC) of 0.79 (95% CI 0.64-0.91) and by the admission shock index with AUROC of 0.79 (95% CI 0.66-0.91). For predicting hypofibrinogenemia, the prehospital shock index ≥ 1 has 0.5 sensitivity (95% CI 0.19-0.81), 0.88 specificity (95% CI 0.83-0.92) and a negative predictive value of 0.98 (0.96-0.99). The shock index may help to identify trauma patients at risk of hypofibrinogenemia early in the prehospital course. more...- Published
- 2023
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34. Pathogenesis of infections caused by SARS-CoV-2.
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Beneš J
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- Humans, Post-Acute COVID-19 Syndrome, SARS-CoV-2, COVID-19
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SARS-CoV-2 primarily causes mucosal infections of the respiratory or intestinal tract. This virus, unlike other viruses responsible for similar mucosal infections, is characterized by an extraordinary ability to modify the immune response at several levels and thus cause a range of clinical complications. These manipulations create a false picture of pyogenic bacterial infection. The course of the disease is mainly determined by the natural mucosal immunity which can stop the virus from multiplying in the early stages of infection before it can exert its influence. COVID-19 has two main clinical forms: mucosal infection (respiratory or intestinal) and pneumonia. Pneumonia is associated with activation of the vascular endothelium and a procoagulant state. Viremia does not belong to the standard course of the disease. Affecting organs other than the lungs - whether during an active infection or later (long covid) - is usually caused by immunopathological reactions or hormonal regulation disorders. more...
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- 2023
35. [Role of chloramphenicol in current clinical practice].
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Džupová O and Beneš J
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- Adult, Humans, Chloramphenicol adverse effects, Anti-Bacterial Agents adverse effects, Bacterial Infections drug therapy, Anti-Infective Agents therapeutic use, Communicable Diseases
- Abstract
Introduction: Chloramphenicol is an antibiotic with a broad spectrum of action and excellent tissue penetration. It had been widely used in clinical practice until the 1970s, but due to its potential myelotoxicity, it was gradually replaced by newly introduced antibiotics in the following years. The aim of the study was to find out to what extent and with what experience it is currently used in the Czech Republic., Methods: A questionnaire survey was conducted in July and August 2022. The heads of all inpatient infectious diseases departments, hospital infectious diseases specialists and consulting microbiologists from antibiotic centers in large teaching hospitals without an infectious diseases department were asked to fill out the questionnaire., Results: Thirty-five out of 39 hospitals contacted took part in the study, a response rate of 90 %. Chloramphenicol is used in 37 % of participating hospitals, with a frequency of up to 10 patients treated per year. The most common indications are brain abscesses, purulent meningitis, intra-abdominal, pelvic and lung abscesses, and polymicrobial infections with anaerobes. Chloramphenicol is almost always administered as an alternative antibiotic because of polyvalent allergy, bacterial resistance, and failure of previous treatment. Sixty-six percent of respondents described the effect as reliable or partially reliable, 34 % did not rate the effect. Fifty-two percent of respondents considered a dose of 8-9 g to be the maximum dose for an adult patient. In practice, 60 % of respondents did not encounter or could not assess the myelotoxic effects of chloramphenicol, 37 % observed reversible bone marrow suppression at least once, and only one respondent encountered aplastic anemia once., Conclusion: Unfortunately, chloramphenicol is currently used in less than half of hospitals in the Czech Republic. Because of its unique properties, it still has a place in today's anti-infective treatment. When properly indicated and after weighing the benefits and risks, it can be a suitable and sometimes life-saving alternative. more...
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- 2022
36. SARS-CoV-2 Specific Humoral Immune Responses after BNT162b2 Vaccination in Hospital Healthcare Workers.
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Golshani M, Svobodová LM, Štěpánek L, Zeman J, Nytrová P, Posová H, Petrásková P, Novotná O, Nováková M, Černý V, Beneš J, Kolářová L, Vokurka M, and Hrdý J
- Abstract
Background: COVID-19 pandemic has led to a loss of human life in millions and devastating socio-economic consequences worldwide. So far, vaccination is the most effective long-term strategy to control and prevent severe COVID-19 disease. The aim of the current study was to evaluate the humoral immune responses raised against the BNT162b2 vaccine in hospital healthcare workers., Methods: Total number of 173 healthcare workers enrolled in the study. Their blood samples were collected in three different time intervals after the second SARS-CoV-2 vaccination and evaluated by the ELISA method to detect anti-spike protein IgM and IgG antibodies. The baseline characteristics of all participants were collected using questionnaires and were evaluated for finding any significant data., Results: Our results demonstrated that the levels of antibodies were higher in the young group (21-30 years old) and also among male participants. Moreover, the highest levels of antibodies were detected from the group that received the third shot vaccination., Conclusions: Our results indicate that age, gender and third-dose vaccination can affect the levels of humoral immune responses against the BNT162b2 vaccine in healthcare workers. more...
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- 2022
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37. Extensive archaeobotanical data estimate carrying capacity, duration, and land use of the Late Bronze Age settlement site Březnice (Czech Republic).
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Šálková T, Vobejda L, Chvojka O, Beneš J, Vondrovský V, Kuna M, Křivánek R, Menšík P, and Novák J
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- Humans, Czech Republic, Forests, Ecosystem, Conservation of Natural Resources, Trees
- Abstract
The reconstruction of the settlement´s hinterland and acquisition of plant resources is one of the crucial questions in the field of environmental archaeology. Our study is focused on the reconstruction of the settlement's structure and character of the environment from which the site drew resources. These research questions were addressed by the interpretation of plant macroremains, charcoals, and the results of the spatial model. We have focused on the maximum size of the settlement that the surrounding countryside was able to withstand. Our results clearly demonstrated significant deforestation and intensive land use in the vicinity of the Late Bronze Age study site. As the weed taxa showed, a wide range of crops was grown in rather dry or less often in damp fields. Based on our archaeobotanical results, we were able to reconstruct several types of grasslands: dry pastures and fallow fields on plateaus and slopes, wet pastures or meadows in the floodplain. Acidophilous oak forests, alluvial forests, and shrubs were reconstructed as the most common forest habitats in the vicinity of the study site. Based on the archaeological knowledge of the region, we assume relatively low population density during the Late Bronze Age, and thus only a small part of the more or less forested landscape was significantly affected by human activities., (© 2022. The Author(s).) more...
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- 2022
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38. Pharmacokinetics of Intramuscularly Administered Thermoresponsive Polymers.
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Groborz O, Kolouchová K, Pankrác J, Keša P, Kadlec J, Krunclová T, Pierzynová A, Šrámek J, Hovořáková M, Dalecká L, Pavlíková Z, Matouš P, Páral P, Loukotová L, Švec P, Beneš H, Štěpánek L, Dunlop D, Melo CV, Šefc L, Slanina T, Beneš J, Van Vlierberghe S, Hoogenboom R, and Hrubý M more...
- Subjects
- Mice, Animals, Tissue Distribution, Temperature, Drug Liberation, Polymers, Water
- Abstract
Aqueous solutions of some polymers exhibit a lower critical solution temperature (LCST); that is, they form phase-separated aggregates when heated above a threshold temperature. Such polymers found many promising (bio)medical applications, including in situ thermogelling with controlled drug release, polymer-supported radiotherapy (brachytherapy), immunotherapy, and wound dressing, among others. Yet, despite the extensive research on medicinal applications of thermoresponsive polymers, their biodistribution and fate after administration remained unknown. Thus, herein, they studied the pharmacokinetics of four different thermoresponsive polyacrylamides after intramuscular administration in mice. In vivo, these thermoresponsive polymers formed depots that subsequently dissolved with a two-phase kinetics (depot maturation, slow redissolution) with half-lives 2 weeks to 5 months, as depot vitrification prolonged their half-lives. Additionally, the decrease of T
CP of a polymer solution increased the density of the intramuscular depot. Moreover, they detected secondary polymer depots in the kidneys and liver; these secondary depots also followed two-phase kinetics (depot maturation and slow dissolution), with half-lives 8 to 38 days (kidneys) and 15 to 22 days (liver). Overall, these findings may be used to tailor the properties of thermoresponsive polymers to meet the demands of their medicinal applications. Their methods may become a benchmark for future studies of polymer biodistribution., (© 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.) more...- Published
- 2022
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39. [Updated Czech guidelines for the treatment of patients with colitis due to Clostridioides difficile].
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Beneš J, Stebel R, Musil V, Krůtová M, Vejmelka J, and Kohout P
- Subjects
- Humans, Vancomycin therapeutic use, Fidaxomicin therapeutic use, Clostridioides, Tigecycline therapeutic use, Czech Republic, Aminoglycosides adverse effects, Anti-Bacterial Agents therapeutic use, Clostridioides difficile, Clostridium Infections drug therapy, Colitis chemically induced, Colitis drug therapy
- Abstract
The updated Czech guidelines differ in some aspects from the 2021 guidelines issued by the ESCMID Study Group for Clostridium difficile. The key points of these Czech recommendations may be summarized as follows: • The drug of choice for hospitalized patients is orally administered fidaxomicin or vancomycin. In outpatients with a mild first episode of C. difficile infection, metronidazole can also be used. • If the patient's response to treatment is good and there are no complications, the duration of antibiotic treatment can be reduced (e.g. to 5 days in case of fidaxomicin or to 6-7 days in case of vancomycin). • If oral therapy is impossible, the drug of choice is tigecycline, 100 mg i.v., b.i.d., with initial shortening of the interval between the first and second doses for faster saturation. If the severity of the disease progresses during this antibiotic treatment, it is necessary to access the ileum or cecum, i.e. to perform double ileostomy or percutaneous endoscopic cecostomy, and to instill vancomycin or fidaxomicin lavages. • Fulminant C. difficile colitis should be treated with oral fidaxomicin ± tigecycline i.v. If peristalsis ceases, fidaxomicin should be administered into the ileum or cecum as described above. If sepsis develops, a broad-spectrum beta-lactam antibiotic (piperacillin/tazobactam, carbapenem) i.v. is added to topically administered fidaxomicin instead of tigecycline i.v.; at the same time, colectomy should be considered as the last resort. • To treat first recurrence, fidaxomicin or vancomycin is administered with a subsequent fecal microbiota transplant (FMT) from a healthy donor. For second or subsequent recurrence, administration of fidaxomicin is of little benefit; the therapy of choice is oral vancomycin and subsequent FMT. Prolonged vancomycin or fidaxomicin taper and pulse treatment is appropriate only when FMT cannot be performed. more...
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- 2022
40. Author Correction: Ecological and historical factors behind the spatial structure of the historical field patterns in the Czech Republic.
- Author
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Fanta V, Beneš J, Zouhar J, Rakava V, Šitnerová I, Janečková Molnárová K, Šmejda L, and Sklenicka P
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- 2022
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41. Determination of stress and displacement on human composite pelvis under static and dynamic loading.
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Beneš J, Kunz L, Liška J, Šrám J, Krbec M, Džupa V, Křivohlávek M, and Návrat T
- Subjects
- Biomechanical Phenomena, Humans, Pelvic Bones, Pelvis
- Abstract
This case study describes the experimental determination of displacements and stresses on a composite model of a pelvis that was modified to represent a healthy intact pelvic ring. The modified model was stressed statically up to 1750 N to simulate standing on one leg and also cyclically to model walking. For two different model settings in the loading machine the values of displacements and stresses at the pelvic ring were determined. The two different settings correspond to two different loading vectors applied on the pelvic ring, boundary conditions and degrees of freedom. The experimentally determined values of displacements in both settings are very similar and in accordance with the knowledge on the behaviour of a real human pelvis. The modified model is thus suitable for testing of newly developed implants for pelvis treatment and experimental determination of displacements and stresses in pelvic ring which are caused by application of implants. more...
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- 2022
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42. Epithelioid sarcoma with retained INI1 expression as a cause of a chronic leg ulcer.
- Author
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Maňáková T, Hojný J, Sedlář M, Vočka M, Chrz K, Mitáš P, Beneš J, and Holaj R
- Abstract
Epithelioid sarcoma is a rare soft-tissue sarcoma typically presenting itself as a subcutaneous or deep dermal mass in distal portions of the extremities of adolescents and young adults. They are frequently mistaken for ulcers, abscesses, or infected warts resistant to standard medical treatment. Patients often develop multiple local recurrences with subsequent metastases. We report a case of a 66-year-old patient with chronic leg ulcer who died of generalization of an epithelioid sarcoma., Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2022.) more...
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- 2022
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43. Ecological and historical factors behind the spatial structure of the historical field patterns in the Czech Republic.
- Author
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Fanta V, Beneš J, Zouhar J, Rakava V, Šitnerová I, Janečková Molnárová K, Šmejda L, and Sklenicka P
- Subjects
- Conservation of Natural Resources, Czech Republic, Ecosystem, Farms, Geography, Humans, Agriculture, Archaeology
- Abstract
Historical field systems are an essential part of the traditional cultural landscape of societies with primarily agricultural subsistence. They embody many functions and values, as they affect the productional, ecological and hydrological functioning of the landscape, its cultural values, the way people perceive the landscape, and their impact on present-day farming. As an aspect of the historical landscape, field systems are a topic investigated in landscape archaeology, environmental studies, historical geography, landscape ecology, and related disciplines. Historical field systems can form many complex spatial structures, shapes and patterns. This paper focuses on identifying environmental and historical/cultural driving forces during the formation and the historical development of various field pattern types. We worked with 523 settlements established in the medieval to the early modern period (approx. 900-1600 AD) in the present-day Czech Republic. We have determined the proportions of different field pattern types in the examined cadastres and have statistically compared them with a variety of environmental and geographical predictors. Our results indicate a strong influence of environmental predictors (terrain undulation, cadastre size), the impact of specific historical events and associated social changes (e.g. land confiscations by the state in the seventeenth century), and a significant relationship between field pattern types and settlement layout types. Furthermore, we have observed the different adaptations of field pattern types to similar environmental conditions, as well as the impact of social and political factors on the processes of landscape formation. Our paper provides the first detailed analysis of the geographical distribution of traditional field systems on the scale of an entire modern state, and emphasizes the importance of transdisciplinary research on cultural landscapes., (© 2022. The Author(s).) more...
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- 2022
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44. Hyperbaric oxygen influences chronic wound healing - a cellular level review.
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Růžička J, Dejmek J, Bolek L, Beneš J, and Kuncová J
- Subjects
- Animals, Heat-Shock Proteins metabolism, Humans, Hypoxia-Inducible Factor 1 metabolism, Reactive Nitrogen Species metabolism, Reactive Oxygen Species metabolism, Treatment Outcome, Wounds and Injuries metabolism, Wounds and Injuries pathology, Hyperbaric Oxygenation, Wound Healing, Wounds and Injuries therapy
- Abstract
Chronic wound is a serious medical issue due to its high prevalence and complications; hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. Clinical trials, including large meta-analyses bring inconsistent results about HBOT efficacy. This review is summarizing the possible effect of HBOT on the healing of chronic wound models at the cellular level. HBOT undoubtedly escalates the production of reactive oxygen and nitrogen radicals (ROS and RNS), which underlie both the therapeutic and toxic effects of HBOT on certain tissues. HBOT paradoxically elevates the concentration of Hypoxia inducible factor (HIF) 1 by diverting the HIF-1 degradation to pathways that are independent of the oxygen concentration. Elevated HIF-1 stimulates the production of different growth factors, boosting the healing process. HBOT supports synthesis of Heat shock proteins (HSP), which are serving as chaperones of HIF-1. HBOT has antimicrobial effect, increases the effectiveness of some antibiotics, stimulates fibroblasts growth, collagen synthesis and suppresses the activity of proteolytic enzymes like matrix metalloproteinases. All effects of HBOT were investigated on cell cultures and animal models, the limitation of their translation is discussed at the end of this review. more...
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- 2021
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45. Hyperbaric oxygen enhances collagen III formation in wound of ZDF rat.
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Růžička J, Grajciarová M, Vištejnová L, Klein P, Tichánek F, Tonar Z, Dejmek J, Beneš J, Bolek L, Bajgar R, and Kuncová J
- Subjects
- Animals, Diabetic Foot metabolism, Male, Random Allocation, Rats, Zucker, Rats, Collagen Type III metabolism, Diabetic Foot therapy, Hyperbaric Oxygenation, Wound Healing
- Abstract
Diabetic foot ulcer (DFU) is a serious complication of diabetes and hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. The evidence supporting the use of HBOT in DFU treatment is controversial. The aim of this work was to introduce a DFU model in ZDF rat by creating a wound on the back of an animal and to investigate the effect of HBOT on the defect by macroscopic evaluation, quantitative histological evaluation of collagen (types I and III), evaluation of angiogenesis and determination of interleukin 6 (IL6) levels in the plasma. The study included 10 rats in the control group (CONT) and 10 in the HBOT group, who underwent HBOT in standard clinical regimen. Histological evaluation was performed on the 18th day after induction of defect. The results show that HBOT did not affect the macroscopic size of the defect nor IL6 plasma levels. A volume fraction of type I collagen was slightly increased by HBOT without reaching statistical significance (1.35+/-0.49 and 1.94+/-0.67 %, CONT and HBOT, respectively). In contrast, the collagen type III volume fraction was ~120 % higher in HBOT wounds (1.41+/-0.81 %) than in CONT ones (0.63+/-0.37 %; p=0.046). In addition, the ratio of the volume fraction of both collagens in the wound ((I+III)w) to the volume fraction of both collagens in the adjacent healthy skin ((I+III)h) was ~65 % higher in rats subjected to HBOT (8.9+/-3.07 vs. 5.38+/-1.86 %, HBOT and CONT, respectively; p=0.028). Vessels density (number per 1 mm2) was found to be higher in CONT vs. HBOT (206.5+/-41.8 and 124+/-28.2, respectively, p<0.001). Our study suggests that HBOT promotes collagen III formation and decreases the number of newly formed vessels at the early phases of healing. more...
- Published
- 2021
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46. What about Dinner? Chemical and Microresidue Analysis Reveals the Function of Late Neolithic Ceramic Pans.
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Beneš J, Todoroska V, Budilová K, Kovárník J, Pavelka J, Atanasoska N, Bumerl J, Florenzano A, Majerovičová T, Vondrovský V, Ptáková M, Bednář P, Richtera L, and Kučera L
- Subjects
- Animals, Archaeology, Ceramics history, Cooking history, Gas Chromatography-Mass Spectrometry, History, Ancient, Plant Extracts history, Proteins history, Republic of North Macedonia, Swine, Ceramics analysis, Food history, Plant Extracts analysis, Proteins analysis
- Abstract
The Late Neolithic palafitte site, Ustie na Drim, in the northern part of Lake Ohrid (North Macedonia), excavated in 1962, offered ceramic fragments of large, flat, elongated pans. These artifacts could be dated by relative chronology to roughly around 5200-5000 BC. According to their shape and technological traits, the ceramic pans were probably used for baking. The attached materials on the surface of studied pan fragments were sampled for consequent chemical and microscopical analyses (i.e., analyses of starch, phytoliths, and microscopic animal remains). An immunological method revealed the presence of pork proteins in samples. The presence of organic residues of animal origin was, moreover, confirmed by the detection of cholesterol using gas chromatography coupled to mass spectrometry. Analysis of detected microscopic botanical objects revealed starch grains of several plants (i.e., oak, cattail, and grasses). An interesting find was the hair of a beetle larva, which could be interpreted contextually as the khapra beetle, a pest of grain and flour. Based on our data, we suppose that the ceramic pans from Ustie na Drim were used for the preparation of meals containing meat from common livestock in combination with cereals and wild plants. more...
- Published
- 2021
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47. [COVID-19: diagnosis and treatment].
- Author
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Štefan M, Chrdle A, Husa P, Beneš J, and Dlouhý P
- Subjects
- Antiviral Agents therapeutic use, Humans, Lung diagnostic imaging, SARS-CoV-2, Ultrasonography, COVID-19
- Abstract
The guidelines provide evidence-based recommendations for the management of COVID-19. The clinical manifestations of the disease are described and indication criteria for hospital admission of patients with COVID-19 are listed. Polymerase chain reaction and antigen testing are used in direct diagnostics. Indirect detection of infection by antibodies is currently of limited value. There are a number of hematological and biochemical laboratory test used to diagnose COVID-19. Pathological values of some laboratory parameters are associated with severity of COVID-19. Of the imaging studies, chest X-ray, chest computer tomography and lung ultrasound are used. COVID-19 therapy includes symptomatic and specific therapy (antivirals, immunotherapeutics and anticoagulants) and intensive care in the severe and critical forms of the disease. Remdesivir and favipiravir are available as antiviral agents. Immunotherapeutics include monoclonal antibodies (casirivimab/imdevimab, bamlanivimab/etesevimab), dexamethas one, baricitinib and tocilizumab. Low-molecular-weight heparin is a dominant form of anticoagulant therapy. The guidelines provide specific therapeutic recommendations for each stage of the disease. Antibiotics are recommended only if bacterial superinfection is suspected or demonstrated, which is not common in the early stages of the disease. more...
- Published
- 2021
48. Restoring a butterfly hot spot by large ungulates refaunation: the case of the Milovice military training range, Czech Republic.
- Author
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Konvička M, Ričl D, Vodičková V, Beneš J, and Jirků M
- Subjects
- Animals, Biodiversity, Cattle, Czech Republic, Horses, Humans, Plants, Butterflies, Military Personnel
- Abstract
Background: Refaunation/rewilding by large ungulates represents a cost-efficient approach to managing natural biotopes and may be particularly useful for areas whose biodiversity depends on disturbance dynamics and is imperilled by successional changes. To study impacts of refaunation on invertebrates, we focused on butterflies inhabiting the former military training range Milovice, Czech Republic, refaunated since 2015 by a combination of Exmoor pony ("wild" horse), Tauros cattle ("aurochs"), and European wisent., Methods: We analysed butterfly presence-absence patterns immediately after the military use termination (early 1990s), prior to the refaunation (2009), and after it (2016-19); and current abundance data gained by monitoring butterflies at refaunated and neglected plots. We used correspondence analysis for the presence-absence comparison and canonical correspondence analysis for the current monitoring, and related results of both ordination methods to the life history and climatic traits, and conservation-related attributes, of recorded butterflies., Results: Following the termination of military use, several poorly mobile species inclining towards oceanic climates were lost. Newly gained are mobile species preferring warmer continental conditions. The refaunated plots hosted higher butterfly species richness and abundances. Larger-bodied butterflies developing on coarse grasses and shrubs inclined towards neglected plots, whereas refaunated plots supported smaller species developing on small forbs., Conclusion: The changes in species composition following the cessation of military use were attributable to successional change, coupled with changes in species pool operating at larger scales. By blocking succession, large ungulates support butterflies depending on competitively poor plants. Restoring large ungulates populations represents a great hope for conserving specialised insects, provided that settings of the projects, and locally adapted ungulate densities, do not deplete resources for species with often contrasting requirements. more...
- Published
- 2021
- Full Text
- View/download PDF
49. Mesenchymal Stromal Cell Therapy in Novel Porcine Model of Diffuse Liver Damage Induced by Repeated Biliary Obstruction.
- Author
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Vištejnová L, Liška V, Kumar A, Křečková J, Vyčítal O, Brůha J, Beneš J, Kolinko Y, Blassová T, Tonar Z, Brychtová M, Karlíková M, Racek J, Mírka H, Hošek P, Lysák D, and Králíčková M
- Subjects
- Animals, Liver Diseases etiology, Liver Diseases pathology, Mesenchymal Stem Cells, Swine, Cholestasis complications, Disease Models, Animal, Liver Diseases therapy, Mesenchymal Stem Cell Transplantation methods
- Abstract
In liver surgery, biliary obstruction can lead to secondary biliary cirrhosis, a life-threatening disease with liver transplantation as the only curative treatment option. Mesenchymal stromal cells (MSC) have been shown to improve liver function in both acute and chronic liver disease models. This study evaluated the effect of allogenic MSC transplantation in a large animal model of repeated biliary obstruction followed by partial hepatectomy. MSC transplantation supported the growth of regenerated liver tissue after 14 days (MSC group, n = 10: from 1087 ± 108 (0 h) to 1243 ± 92 mL (14 days); control group, n = 11: from 1080 ± 95 (0 h) to 1100 ± 105 mL (14 days), p = 0.016), with a lower volume fraction of hepatocytes in regenerated liver tissue compared to resected liver tissue (59.5 ± 10.2% vs. 70.2 ± 5.6%, p < 0.05). Volume fraction of connective tissue, blood vessels and bile vessels in regenerated liver tissue, serum levels of liver enzymes (AST, ALT, ALP and GGT) and liver metabolites (albumin, bilirubin, urea and creatinine), as well as plasma levels of IL-6, IL-8, TNF-α and TGF-β, were not affected by MSC transplantation. In our novel, large animal (pig) model of repeated biliary obstruction followed by partial hepatectomy, MSC transplantation promoted growth of liver tissue without any effect on liver function. This study underscores the importance of translating results between small and large animal models as well as the careful translation of results from animal model into human medicine. more...
- Published
- 2021
- Full Text
- View/download PDF
50. COVID-19 severe pneumonia: Prospective multicentre study on demands on intensive care capacities.
- Author
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Džupová O, Moravec M, Bartoš H, Brestovanský P, Tencer T, Hyánek T, Beroušek J, Krupková Z, Mošna F, Vymazal T, and Beneš J
- Subjects
- Adult, Aged, Critical Care, Female, Hospital Mortality, Humans, Male, Prospective Studies, SARS-CoV-2, COVID-19
- Abstract
Objectives: The aim of the study was to obtain data on demands on the intensive care capacities to treat COVID-19 patients, and to identify predictors for in-hospital mortality., Methods: The prospective observational multicentre study carried out from 1 March till 30 June 2020 included adult patients with confirmed SARS-CoV-2 infection with respiratory failure requiring ventilatory support or high-flow nasal oxygen therapy (HFNO)., Results: Seventy-four patients, 46 males and 28 females, median age 67.5 (Q1-Q3: 56-75) years, were included. Sixty-four patients (86.5%) had comorbidity. Sixty-six patients (89.2%) were mechanically ventilated, four of them received extracorporeal membrane oxygenation therapy. Eight patients (10.8%) were treated with non-invasive ventilation and HFNO only. The median of intensive care unit (ICU) stay was 22.5 days. Eighteen patients (24.3%) needed continuous renal replacement therapy. Thirty patients (40.5%) died. Age and acute kidney injury were identified as independent predictors of in-hospital death, and chronic kidney disease showed trend towards statistical significance for poor outcome., Conclusions: Sufficient number of intensive care beds, organ support equipment and well-trained staff is a decisive factor in managing the COVID-19 epidemic. The study focused on the needs of intensive care in the COVID-19 patients. Advanced age and acute kidney injury were identified as independent predictors for in-hospital mortality. When compared to clinical course and ICU management of patients with severe community-acquired pneumonia caused by other pathogens, we observed prolonged need for ventilatory support, high rate of progression to acute respiratory distress syndrome and significant mortality in studied population. more...
- Published
- 2021
- Full Text
- View/download PDF
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