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PDF file - 65K, Treatment with cisplatin and radiation therapy increases IAP gene expression in additional medulloblastoma cell lines

PDF file - 257K, Treatment with irradiation and LBW242 leads to apoptosis in medulloblastoma cell lines

PDF file - 47K, Supplementary Table 1 The combinatorial index of medulloblastoma cell lines treated with a combination of 1uM LBW-242 and 2 Gy (Daoy, D283) or 4Gy (UW228) irradiation in a colony formation assay. A CI of < 1 denotes a synergistic interaction, a CI of 1 +/-0.2 denotes an additive interaction, and a CI of > 1 indicates an antagonistic interaction. Supplementary Table 2: Clinical characteristics of patients from which short term primary tumor cultures were derived.Supplementary Table 3: The ID50 of medulloblastoma cell lines and primary cultures treated with LBW-242 and Cisplatin, calculated from synergy assay curves using interpolated x values (Graph Pad Prism 5). (IA = Inactive as single agent; N/A = not applicable.)

Neuroblastoma is a frequently lethal childhood tumor in which MYC gene deregulation, commonly as MYCN amplification, portends poor outcome. Identifying the requisite biopathways downstream of MYC may provide therapeutic opportunities. We used transcriptome analyses to show that MYCN-amplified neuroblastomas have coordinately deregulated myriad polyamine enzymes (including ODC1, SRM, SMS, AMD1, OAZ2, and SMOX) to enhance polyamine biosynthesis. High-risk tumors without MYCN amplification also overexpress ODC1, the rate-limiting enzyme in polyamine biosynthesis, when compared with lower-risk tumors, suggesting that this pathway may be pivotal. Indeed, elevated ODC1 (independent of MYCN amplification) was associated with reduced survival in a large independent neuroblastoma cohort. As polyamines are essential for cell survival and linked to cancer progression, we studied polyamine antagonism to test for metabolic dependence on this pathway in neuroblastoma. The Odc inhibitor α-difluoromethylornithine (DFMO) inhibited neuroblast proliferation in vitro and suppressed oncogenesis in vivo. DFMO treatment of neuroblastoma-prone genetically engineered mice (TH-MYCN) extended tumor latency and survival in homozygous mice and prevented oncogenesis in hemizygous mice. In the latter, transient Odc ablation permanently prevented tumor onset consistent with a time-limited window for embryonal tumor initiation. Importantly, we show that DFMO augments antitumor efficacy of conventional cytotoxics in vivo. This work implicates polyamine biosynthesis as an arbiter of MYCN oncogenesis and shows initial efficacy for polyamine depletion strategies in neuroblastoma, a strategy that may have utility for this and other MYC-driven embryonal tumors. [Cancer Res 2008;68(23):9735–45]

Supplementary Figure 3 from ODC1 Is a Critical Determinant of MYCN Oncogenesis and a Therapeutic Target in Neuroblastoma

Supplementary Figure 1 from ODC1 Is a Critical Determinant of MYCN Oncogenesis and a Therapeutic Target in Neuroblastoma

Supplementary Figure Legends 1-3 from ODC1 Is a Critical Determinant of MYCN Oncogenesis and a Therapeutic Target in Neuroblastoma