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This work investigated non-polar solvent hexane and polar solvents methanol and ethanol as inducers besides a well-known inducer, copper, for laccase production with and without mesoporous silica-covered plastic packing under sterilised and unsterilised conditions. The potential of waste-hexane water, which is generated during the mesoporous silica production process, was also investigated as a laccase inducer. During the study, the free and immobilised laccase activity on the packing was measured. The results showed that the highest total laccase activity, approximately 10,000 Units, was obtained under sterilised conditions with 0.5 mM copper concentration. However, no immobilised laccase activity was detected except in the copper and ethanol sets under unsterilised conditions. The maximum immobilised laccase activity of the sets that used waste hexane as an inducer was 1.25 U/mg packing. According to its significant performance, waste hexane can be an alternative inducer under sterilised conditions. Concomitant immobilised packing showed satisfactory laccase activities and could be a promising method to reduce operation costs and improve the cost-efficiency of enzymatic processes in wastewater treatment plants. [ABSTRACT FROM AUTHOR]

Global warming needs immediate attention to reduce major greenhouse gas emissions such as methane (CH4). Bio-oxidation of dilute CH4 emissions in packed-bed bioreactors such as biofilters has been carried out over recent years at laboratory and large scales. However, a big challenge is to keep CH4 biofilters running for a long period. In this study, a packed-bed lab-scale bioreactor with a specialized inorganic-based filter bed was successfully operated over four years for CH4 elimination. The inoculation of the bioreactor was the active leachate of another CH4 biofilter which resulted in a fast acclimation and removal efficiency (RE) reached 80% after seven weeks of operation for CH4 inlet concentrations ranging from 700 to 800 ppmv and an empty bed residence time (EBRT) of 6 min. During four years of operation, the bioreactor often recorded REs higher than 65% for inlet concentrations in the range of 1900–2200 ppmv and an EBRT of 6 min. The rate and interval of the nutrient supply played an important role in maintaining the bioreactor's high performance over the long operation. Forced shutdowns were unavoidable during the 4-year operation and the bioreactor fully tolerated them with a partial recovery within one week and a progressive recovery over time. In the end, the bioreactor's filter bed started to deteriorate due to a long shutdown of twelve weeks and the extended operation of four years when the RE dropped to below 8% with no sign of returning to its earlier performance. [ABSTRACT FROM AUTHOR]

Hazardous airborne pollutants are frequently emitted to the atmosphere in the form of a gaseous mixture. Air biofilters as the primary biotechnological choice for waste gas treatment (low inlet concentration and high gas flow rate) should run properly when the feed contains multiple pollutants. Simultaneous removal of pollutants in biofilters has been extensively studied over the last 10 years. In this review, the results and findings of the mentioned studies including different groups of pollutants, such as methane (CH4) and volatile organic compounds (VOCs) are discussed. As the number of pollutants in a mixture increases, their elimination might become more complicated due to interactions between the pollutants. Parallel batch studies might be helpful to better understand these interaction effects in the absence of mass transfer limitations. Setting optimum operating conditions for removal of mixtures in biofilters is challenging because of opposing properties of pollutants. In biofilters, concerns, such as inlet gas composition variation and stability while dealing with abrupt inlet load and concentration changes, must be managed especially at industrial scales. Biofilters designed with multi-layer beds, allow tracking the fate of each pollutant as well as analyzing the diversity of microbial culture across the filter bed. Certain strategies are recommended to improve the performance of biofilters treating mixtures. For example, addition of (bio)surfactants as well as a second liquid phase in biotrickling filters might be considered for the elimination of multiple pollutants especially when hydrophobic pollutants are involved. [ABSTRACT FROM AUTHOR]

Sewage from residents and industries is collected and transported to wastewater treatment plants (WWTPs) with sewer networks. The operation of WWTPs results in emissions of greenhouse gases, such as methane (CH4), mostly due to sludge anaerobic digestion. Amounts of emissions depend on the source of influent, i.e. municipal and industrial wastewater as well as sewer systems (gravity and rising). Wastewater is the fifth-largest source of anthropogenic CH4 emissions in the world and represents 7–9% of total global CH4 emissions into the atmosphere. Global wastewater CH4 emission grew by approximately 20% from 2005 to 2020 and is expected to grow by 8% between 2020 and 2030, which makes wastewater an important CH4 emitter worldwide. This review initially considers the emission of CH4 from WWTPs and sewer networks. In the second part, biotechniques available for biodegradation of low CH4 concentrations (<5% v/v) encountered in WWTPs have been studied. The paper reviews major bioreactor configurations for the treatment of polluted air, i.e. biotrickling filters, bioscrubbers, two-liquid phase bioreactors, biofilters, and hybrid reactor configurations, after which it focuses on CH4 biofiltration systems. Biofiltration represents a simple and efficient approach to bio-oxidize CH4 in waste gases from WWTPs. Major factors influencing a biofilter's performance along with knowledge gaps in relation to its application for treating gaseous emissions from WWTPs are discussed. [ABSTRACT FROM AUTHOR]