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Your search keyword '"Kolodkin-Gal, Ilana"' showing total 224 results
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224 results on '"Kolodkin-Gal, Ilana"'

14. Voices of biotech research

Author

Annabi, Nasim, Baker, Matthew, Boettiger, Alistair, Chakraborty, Debojyoti, Chen, Yvonne, Corbett, Kizzmekia S., Correia, Bruno, Dahlman, James, de Oliveira, Tulio, Ertuerk, Ali, Yanik, Mehmet Fatih, Henaff, Elizabeth, Huch, Meritxell, Iliev, Iliyan D., Jacobs, Thomas, Junca, Howard, Keung, Albert, Kolodkin-Gal, Ilana, Krishnaswamy, Smita, Lancaster, Madeline, Macosko, Evan, Martínez-Núñez, Mario Alberto, Miura, Kyoko, Molloy, Jenny, Cruz, Andrés Ochoa, Platt, Randall J., Posey, Jr., Avery D., Shao, Huilin, Simunovic, Mijo, Slavov, Nikolai, Takebe, Takanori, Vandenberghe, Luk H., Varshney, Rajeev K., and Wang, Jianbin

Published
2021
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15. Toxin‐Antitoxin systems eliminate defective cells and preserve symmetry in Bacillus subtilis biofilms

Author

Bloom‐Ackermann, Zohar, Steinberg, Nitai, Rosenberg, Gili, Oppenheimer‐Shaanan, Yaara, Pollack, Dan, Ely, Shir, Storzi, Nimrod, Levy, Asaf, and Kolodkin‐Gal, Ilana

Subjects
Microbiology, Biological Sciences, Infection, Antitoxins, Bacillus subtilis, Bacterial Proteins, Bacterial Toxins, Biofilms, Evolutionary Biology, Ecology
Abstract

Toxin-antitoxin modules are gene pairs encoding a toxin and its antitoxin, and are found on the chromosomes of many bacteria, including pathogens. Here, we characterize the specific contribution of the TxpA and YqcG toxins in elimination of defective cells from developing Bacillus subtilis biofilms. On nutrient limitation, defective cells accumulated in the biofilm breaking its symmetry. Deletion of the toxins resulted in accumulation of morphologically abnormal cells, and interfered with the proper development of the multicellular community. Dual physiological responses are of significance for TxpA and YqcG activation: nitrogen deprivation enhances the transcription of both TxpA and YqcG toxins, and simultaneously sensitizes the biofilm cells to their activity. Furthermore, we demonstrate that while both toxins when overexpressed affect the morphology of the developing biofilm, the toxin TxpA can act to lyse and dissolve pre-established B. subtilis biofilms.

Published
2016

17. The Utilization of Bacillus subtilis to Design Environmentally Friendly Living Paints with Anti-Mold Properties.

Author

Dorfan, Yuval, Nahami, Avichay, Morris, Yael, Shohat, Benny, and Kolodkin-Gal, Ilana

Subjects
BACILLUS subtilis, MOLDS (Fungi), BACILLUS (Bacteria), HYPERSENSITIVITY pneumonitis, AGRICULTURAL ecology, ASPERGILLUS niger
Abstract

The anti-fungal properties of the probiotic bacterium Bacillus subtilis have been studied extensively in agriculture and ecology, but their applications in the built environment remain to be determined. Our work aims to utilize this biological component to introduce new diverse anti-mold properties into paint. "Mold" refers to the ubiquitous fungal species that generate visible multicellular filaments commonly found in household dust. The development of mold leads to severe health problems for occupants, including allergic response, hypersensitivity pneumonitis, and asthma, which have significant economic and clinical outcomes. We here demonstrate the robust effect of a commercial paint enhanced with Bacillus subtilis cells against the common mold agent, Aspergillus niger, and identify three biosynthetic clusters essential for this effect. Our results lay the foundation for bio-convergence and synthetic biology approaches to introduce renewable and environmentally friendly bio-anti-fungal agents into the built environment. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Editorial: Women in microbial physiology and metabolism: 2023.

Author

Addis, Maria Filippa, Jie Xiao, and Kolodkin-Gal, Ilana

Subjects
MICROBIAL physiology, PHYSIOLOGY of women, MICROBIAL metabolism, QUORUM sensing, MICROBIAL metabolites, HORIZONTAL gene transfer, LACTIC acid bacteria
Abstract

This editorial highlights the significant contributions of female scientists to the field of microbial physiology and metabolism. It discusses historical discoveries made by female scientists, as well as recent studies led by women in the field. The article emphasizes the importance of diversity and inclusion in the scientific community. It also addresses the issue of bias in microbiology research and encourages readers to critically evaluate information they come across. This article provides a valuable perspective for library patrons conducting research on microbiology. [Extracted from the article]

Published
2024
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38. Generating heterokaryotic cells via bacterial cell-cell fusion

Author

Shitut, S.S., Shen, M., Claushuis, B., Derks, R.J.E., Giera, M., Rozen, D.E., Claessen, D., Kros, A., and Kolodkin-Gal Ilana

Subjects
Protocell, Microbiology (medical), Physiology, Cell, heterokaryon, Membrane Fusion, Genetic recombination, Bacterial cell structure, Cell wall, medicine, Genetics, cell membranes, Heterokaryon, Fusion, Cell fusion, cell fusion, Bacteria, General Immunology and Microbiology, Ecology, Chemistry, membrane fluidity, Cell Biology, cell wall deficient, protoplast fusion, lipopeptides, Anti-Bacterial Agents, Cell biology, medicine.anatomical_structure, Infectious Diseases, wall deficiency, coiled-coil peptides, Peptides
Abstract

Cell-cell fusion is instrumental in introducing different sets of genes in the same environment, which subsequently leads to diversity. There is need for new protocols to fuse cells of different types together for biotechnological applications like drug discovery.Fusion of cells is an important and common biological process that leads to the mixing of cellular contents and the formation of multinuclear cells. Cell fusion occurs when distinct membranes are brought into proximity of one another and merge to become one. Fusion holds promise for biotechnological innovations, for instance, for the discovery of urgently needed new antibiotics. Here, we used antibiotic-producing bacteria that can proliferate without their cell wall as a model to investigate cell-cell fusion. We found that fusion between genetically distinct cells yields heterokaryons that are viable, contain multiple selection markers, and show increased antimicrobial activity. The rate of fusion induced using physical and chemical methods was dependent on membrane fluidity, which is related to lipid composition as a function of cellular age. Finally, by using an innovative system of synthetic membrane-associated lipopeptides, we achieved targeted fusion between distinctly marked cells to further enhance fusion efficiency. These results provide a molecular handle to understand and control cell-cell fusion, which can be used in the future for the discovery of new drugs. IMPORTANCE Cell-cell fusion is instrumental in introducing different sets of genes in the same environment, which subsequently leads to diversity. There is need for new protocols to fuse cells of different types together for biotechnological applications like drug discovery. We present here wall-deficient cells as a platform for the same. We identify the fluidity of the membrane as an important characteristic for the process of fusion. We demonstrate a cell-specific approach for fusion using synthetically designed peptides yielding cells with modified antibiotic production profiles. Overall, wall-deficient cells can be a chassis for innovative metabolite production by providing an alternative method for cell-cell fusion.

Published
2022

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